Akshay Garg

Background: Mycobacteroides chelonae M77 isolated in a targeted Mycobacterial isolation study from bovine raw milk was whole genome sequenced (CP041150.1), followed by comparative genomics study for better understanding of the organism with special focus on its virulence and antibiotic resistance pattern. This is probably the first whole genome seq...

Mycobacterium fortuitum belongs to the group of Nontuberculous Mycobacteria (NTM) which falls under the genus Mycobacterium. Ubiquitous in nature, most of the NTM are known to overcome the barrier of disinfectants and cause opportunistic infections. In this study, 35 water samples were collected from seven districts of Punjab and Chandigarh which w...

Owing to the fact that raw milk intake is still practiced in selected communities globally, milk borne diseases need to be addressed as some mycobacterial species use milk as a ploy for disseminating the disease. An approach was made in the present study for screening the mycobacterial species in raw cow milk. For mycobacterial isolation, a total o...

Initially diagnosed with cervical lymphadenitis, a 15-year-old boy was started with category I anti-tuberculosis (TB) drugs. Follow-up investigations led to isolation and identification of Mycobacterium lentiflavum by multiple diagnostic and identification approaches. Observation of this rare pathogen from human origin urges cautious diagnosis whil...

Fibronectin attachment protein (FAP) also known as ModD gene of 987 bp size was identified in the Mycobacterium avium subsp. paratuberculosis (MAP) genome. This gene was successfully amplified by PCR using specific primers and MAP genomic DNA template, in the presence of 10% DMSO and glycerol. The PCR product was cloned in pGEMT easy cloning vector...

Electromagnetic waves are used in today lives and spreading the environmental factor. We need visible light to see, radio waves for telecommunication and x-ray for medical purpose. Electromagnetic radiations are used for its bactericidal effect. It affects the cellular mechanism, cell membrane and might increase or decrease the activity of protein....

Background Frequently isolated in clinical settings and environmental sources, members of the Mycobacterium avium-intracellulare complex (MAIC) comprising genetically distinct species and subtypes holds significant agents responsible for opportunistic infections. The work is focused on molecular identification of a novel Mycobacterium speci...

Garlic (Allium sativum) has important dietary and medicinal role since history. The present study focused on the significant antibacterial activity of Garlic extract on various pathogenic and non-pathogenic bacteria (gram positive as well as gram negative):Staphylococcus aureus, Escherichia coli,Bacillus megaterium, and Pseudomonas aeruginosa. The...

Purified protein derivative (PPD) tuberculin was prepared from Mycobacterium bovis 3/86 and 4/86 strains isolated from Indian cattle. These M. bovis strains were harvested in bulk by growing them in Dorset Henley's liquid medium. PPD of M. bovis AN5 from Veterinary Laboratory Agency, Surrey, UK, was used as control. The PPD produced from M. bovis (...