Akon Higuchi

Akon Higuchi
National Central University | NCU · Department of Chemical & Materials Engineering

PhD

About

318
Publications
58,001
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8,869
Citations
Additional affiliations
April 2007 - present
National Central University
Position
  • Chair

Publications

Publications (318)
Article
The transplantation of human mesenchymal stem cells (hMSCs), such as bone marrow stem cells (BMSCs) and adipose-derived stem cells (ADSCs), has shown beneficial effects in protecting transplanted tissues and cells...
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Cancer stem cells (CSCs) or cancer-initiating cells (CICs) are key factors for tumor generation and metastasis. We investigated a filtration method to enhance CSCs (CICs) from colon carcinoma HT-29 cells and primary colon carcinoma cells derived from patient colon tumors using poly(lactide-co-glycolic acid)/silk screen (PLGA/SK) filters. The colon...
Article
Human pluripotent stem cells (hPSCs) are typically cultivated on extracellular matrix (ECM) protein-coated dishes in xeno-free culture conditions. We supplemented mixed ECM proteins (laminin-511 and recombinant vitronectin, rVT) in culture medium for hPSC culture on conventional polystyrene dishes. Three hPSC cell lines were successfully cultivated...
Article
We developed poly(vinyl alcohol-co-itaconic acid) (PV) hydrogels grafted with laminin-derived peptides that had different joint segments and several specific designs, including dual chain motifs. PV hydrogels grafted with a peptide derived from laminin-β4 (PMQKMRGDVFSP) containing a joint segment, dual chain motif and cationic amino acid insertion...
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Extensive clinical efforts have been made to control the severity of dengue diseases, however, the dengue morbidity and mortality have not declined. Dengue virus can infect and cause systemic damage in many organs, resulting in organ failure. Here, we present a novel report showing a tailored stem cell-based therapy which can aid in viral clearance...
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Due to the limitations in autogenous nerve grafting or Schwann cell transplantation, large gap peripheral nerve injuries require a bridging strategy supported by nerve conduit. Cell based therapies provide a novel treatment for peripheral nerve injuries. In this study, we first experimented an optimal scaffold material synthesis protocol, from wher...
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Introduction It is important to prepare ‘hypoimmunogenic’ or ‘universal’ human pluripotent stem cells (hPSCs) with gene‐editing technology by knocking out or in immune‐related genes, because only a few hypoimmunogenic or universal hPSC lines would be sufficient to store for their off‐the‐shelf use. However, these hypoimmunogenic or universal hPSCs...
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There is a need to store very large numbers of conventional human pluripotent stem cell (hPSC) lines for their off‐the‐shelf usage in stem cell therapy. Therefore, it is valuable to generate “universal” or “hypoimmunogenic” hPSCs with gene‐editing technology by knocking out or in immune‐related genes. A few universal or hypoimmunogenic hPSC lines s...
Article
Cancer-initiating cells (CICs) or cancer stem cells (CSCs) are primarily responsible for tumor initiation, growth, and metastasis and represent a few percent of the total tumor cell population. We designed a membrane filtration protocol to enrich CICs (CSCs) from the LoVo colon cancer cell line via nylon mesh filter membranes with 11 and 20 μm pore...
Article
Thermoresponsive surfaces enable the detachment of cells or cell sheets by decreasing the temperature of the surface when harvesting the cells. However, human pluripotent stem cells (hPSCs), such as embryonic stem cells and induced pluripotent stem cells, cannot be directly cultured on a thermoresponsive surface; hPSCs need a specific extracellular...
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Avascular necrosis (AVN) of the bones remains a major clinical challenge. Fractures in the talus, the scaphoid, and the neck of the femur are especially challenging to heal due to the low blood vessel network and the lack of collateral blood supply. These fractures are associated with high rates of nonunion and increased infections that require rep...
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Human adipose-derived stem cells (hASCs) cultured for 5 passages were filtered through nylon (NY) mesh filter membranes coated with and without extracellular matrix proteins to obtain the permeation solution. Subsequently, the culture media were filtered via the membranes to obtain the recovery solution. Then, the membranes were cultured in cell cu...
Article
Human mesenchymal stem cells, such as human adipose-derived stem cells (hASCs), are typically cultured on a two-dimensional (2D) monolayer material surface, on which 2D culturing methods are easily performed and time-saving. However, hASCs usually suffer from decreased pluripotency and differentiation ability when cultured with a 2D monolayer cultu...
Article
The current differentiation process of human pluripotent stem cells (hPSCs) into cardiomyocytes to enhance the purity of hPSC-derived cardiomyocytes requires some purification processes, which are laborious processes. We developed cell sorting plates, which are prepared from coating thermoresponsive poly(N-isopropylacrylamide) and extracellular mat...
Article
Blindness and vision impairment are caused by irremediable retinal degeneration in affected individuals worldwide. Cell therapy for a retinal replacement can potentially rescue their vision, specifically for those who lost the light sensing photoreceptors in the eye. As such, well-characterized retinal cells are required for the replacement purpose...
Article
Current xeno-free and chemically defined methods for the differentiation of hPSCs (human pluripotent stem cells) into cardiomyocytes are not efficient and are sometimes not reproducible. Therefore, it is necessary to develop reliable and efficient methods for the differentiation of hPSCs into cardiomyocytes for future use in cardiovascular research...
Article
Human mesenchymal stem cells (hMSCs), such as human adipose-derived stem cells (hADSCs), present heterogeneous characteristics, including varying differentiation abilities and genotypes. hADSCs isolated under different conditions exhibit differences in stemness. We isolated hADSCs from human fat tissues via culture on different cell culture biomate...
Article
Recombinant vitronectin-grafted hydrogels were developed by adjusting surface charge of the hydrogels with grafting of poly-L-lysine for optimal culture of human embryonic stem cells (hESCs) in xeno- and feeder-free culture conditions, with elasticity regulated by crosslinking time (10-30 kPa), in contrast to conventional recombinant vitronectin co...
Article
Commonly, stem cell culture is based on batch-type culture, which is laborious and expensive. We continuously cultured human pluripotent stem cells (hPSCs) on thermoresponsive dish surfaces, where hPSCs were partially detached on the same thermoresponsive dish by decreasing the temperature of the thermoresponsive dish to be below the lower critical...
Article
Spinal cord injury (SCI) is a common, severe damage to the central nervous system. Here, we discuss the use of biomaterials for stem cell transplantation in preclinical and clinical studies for the treatment of patients with SCI, because cell culture materials could influence the differentiation fate of stem cells, and not act only as carriers or s...
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It is widely accepted that stem cells directly sense the elasticity of two-dimensional (2-D) substrates and differentiate into a distinct cell type dependent on the substrate elasticity ( direct-sensing differentiation : soft and hard substrates promote differentiation into soft and hard tissue lineage, respectively). Biologically, native extracell...
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The immobilization of proteins on nano-colloidal substrates recently received increasing attention as it may improve the polymer properties and extend their application fields. The present investigation outlined that silkworm silk can represent a potential biopolymer useful to produce nanogold bioconjugates and gold nanoparticles. The gold nanopart...
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Introduction Anti-tuberculosis agent rifampicin is extensively used for its effectiveness. Possible complications of tuberculosis and prolonged rifampicin treatment include kidney damage; these conditions can lead to reduced efficiency of the affected kidney and consequently to other diseases. Bone marrow-derived mesenchymal stem cells (BMMSCs) can...
Chapter
The fate of stem cell differentiation is guided by several different factors of the stem cell microenvironment, such as cell culture biomaterial elasticity (physical cues) and cell–biomaterial interactions (biological cues). Mimicking the stem cell microenvironment using polymer hydrogels with optimal elasticities is an excellent strategy for stem...
Article
To determine the effect of DENV (serotype 2) repeated infections on lung cells is the main goal of this study. From the result, lung histology of control BALB/c mice showed normal alveolar morphology, while vehicle control BALB/c mice highlighted a slight thickening of the alveolar septum. Lung histopathology of BALB/c mice infected twice by DENV-2...
Article
This study sheds light on the effect of the zwitterionization degree (ZD) of alternate copolymers on the antifouling properties of coated polyvinylidene fluoride (PVDF) membranes. A series of copolymers was formed from poly (maleic anhydride-alt-1-octadecene) which underwent a ring opening reaction leading to zwitterionization, and used as coating...
Article
BACKGROUND: Cartilage tissue engineering (CTE) aims to obtain a structure mimicking native cartilage tissue through the combination of relevant cells, three-dimensional scaffolds, and extraneous signals. Implantation of ‘matured’ constructs is thus expected to provide solution for treating large injury of articular cartilage. Type I collagen is wid...
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Poly(ethylene terephtalate) (PET)-based materials face general biofouling issues that we addressed by grafting a copolymer of glycidyl methacrylate and sulfobetaine methacrylate, poly(GMA-r-SBMA). The grafting procedure involved a dip-coating step followed by UV-exposure and led to successful grafting of the copolymer as evidenced by XPS and zeta-p...
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Amphiphilic zwitterionic copolymers have a severe problem of solubility, which restricts the feasibility and development of antifouling coatings. In this work, zwitterionic polymers were grafted via a novel modification method, namely, in situ self-assembling coating (ISC), which consists of synthesizing and simultaneously coating a copolymer onto...
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The rapid spread of highly aggressive arboviruses, parasites, and bacteria along with the development of resistance in the pathogens and parasites, as well as in their arthropod vectors, represents a huge challenge in modern parasitology and tropical medicine. Eco-friendly vector control programs are crucial to fight, besides malaria, the spread of...
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The development of novel mosquito control tools is a key prerequisite to build effective and reliable Integrated Vector Management strategies. Here, we proposed a novel method using cigarette butts for the synthesis of Ag nanostructures toxic to young instars of the malaria vector Anopheles stephensi, chloroquine (CQ)-resistant malaria parasites Pl...
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Anopheles stephensi acts as vector of Plasmodium parasites, which are responsible for malaria in tropical and subtropical areas worldwide. Currently, malaria management is a big challenge due to the presence of insecticide-resistant strains as well as to the development of Plasmodium species highly resistant to major antimalarial drugs. Therefore,...
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The control of filariasis vectors has been enhanced in several areas, but there are main challenges, including increasing resistance to insecticides and lack of cheap and eco-friendly products. The toxicity of iron (Fe⁰) and iron oxide (Fe2O3) nanoparticles has been scarcely investigated yet. We studied the larvicidal and pupicidal activity of Fe⁰...
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Anopheles stephensi is a mosquito vector of malaria, which is still considered a relevant public health problem due to increasing outdoor transmission, growing resistance to insecticides used to target vectors, and antiplasmodial drugs as well. Thus, there is a vital need to explore novel sources of effective compounds. In this study, the hydrother...
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Ocular microbial infection has emerged as a major public health crisis during the past two decades. A variety of causative agents can cause ocular microbial infections; which are characterized by persistent and destructive inflammation of the ocular tissue; progressive visual disturbance; and may result in loss of visual function in patients if ear...
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Physiological environment is a pivotal environment factor in biomedical systems and very easily organized in both in vitro and in vivo. The pH has emerged as a powerful strategy in cancer therapy because it has a profound effect on the interaction of polymeric based drug delivery system with tumor cells. In this regards, the enhancement of pH respo...
Article
The effect of physical cues, such as the stiffness of biomaterials on the proliferation and differentiation of stem cells, has been investigated by several researchers. However, most of these investigators have used polyacrylamide hydrogels for stem cell culture in their studies. Therefore, their results are controversial because those results migh...
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Green fabricated nanoparticles often need to be encapsulated and stabilized, to ensure uniform dispersion in the aquatic environment and relevant larvicidal activity over time. However, recent research showed that nanoencapsulation processes led to a reduction of nanoparticle larvicidal efficacy. We used an extract of Argemone mexicana to reduce Ti...
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Gold nanoparticles (AuNPs) have been extensively studied for applications in biomedical sciences. AuNPs have exceptional advantages in cells studies include good biocompatibility and very low cytotoxicity. The eco-friendly synthesis of AuNPs with various physical characteristics is simple, and led to the fabrication of nanomaterials showing differe...
Chapter
Nowadays, the timely and effective management of mosquito-borne diseases is a crucial public health challenge to deal with. Dengue is an emerging mosquito-borne infectious disease transmitted by Aedes species. Around 3.9 billion people from more than 128 countries were affected by dengue fever. Recent research outlined that dengue can damage the pl...
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Camptothecin (CPT) is an anti-cancer drug that effectively treats various cancers, including colon cancer. However, poor solubility and other drawbacks have restricted its chemotherapeutic potential. To overcome these restrictions, CPT was encapsulated in CEF (cyclodextrin-EDTA-FE3O4), a composite nanoparticle of magnetic iron oxide (Fe3O4), and β-...
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We portray a novel way to deal with the synthesis of iron oxide magnetic nanoparticle incorporated β-cyclodextrin (β-CD) nanocarrier stabilized by ethylenediamine tetra acetic acid (EDTA), which obtained remarkable biocompatibility and biodegradability. The functionality, crystalline nature, morphology and thermal decomposition nature of Fe3O4, β-C...
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An introduction from our Editor-in-Chief, Prof. Akon Higuchi launching Progress in Bioscience and Bioengineering
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An introduction from our Editor-in-Chief, Prof. Akon Higuchi launching Progress in Bioscience and Bioengineering
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Leptospirosis is a neglected zoonosis currently known as reemerging disease worldwide.
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Ticks serve as vectors of a wide range of infectious agents deleterious to humans and animals. Tick bite prevention is based to a large extent on the use of chemical repellents and acaricides. However, development of resistance in targeted ticks, environmental pollution, and contamination of livestock meat and milk are major concerns. Recently, met...
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Cardiovascular disease remains the leading cause of death and disability in advanced countries. Stem cell transplantation has emerged as a promising therapeutic strategy for acute and chronic ischemic cardiomyopathy. The current status of stem cell therapies for patients with myocardial infarction is discussed from a bioengineering and biomaterial...
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Leptospirosis is a widespread zoonotic disease caused by pathogenic Leptospira species (Leptospiraceae). LipL32 is a highly abundant lipoprotein from the outer membrane proteins (OMPs) group, which highly conserved among pathogenic and intermediate Leptospira species. Several studies used LipL32 as a specific gene to identify the presence of leptos...
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The employ of nano-formulated biopesticides for mosquito control represents a promising strategy. Recently, it has been outlined that differences in the green reducing extracts used in nanosynthesis led to the production of nanoparticles with distinct bio-physical properities. In the present investigation, Hedychium coronarium-synthesized silver na...
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Current clinical trials that evaluate human pluripotent stem cell (hPSC)-based therapies predominantly target treating macular degeneration of the eyes because the eye is an isolated tissue that is naturally weakly immunogenic. Here, we discuss current bioengineering approaches and biomaterial usage in combination with stem cell therapy for macular...
Article
Human amniotic fluid-derived stem cells (hAFCs) are pluripotent fetal cells capable of differentiating into multiple lineages, including cell types of each of the three embryonic germ layers. Proper differentiation and maintenance of pluripotency, the defining characteristics of stem cells, are regulated not only by the cells themselves but also by...
Article
This work proposes a new molecular insight of interfacial design in the control of antifouling performance for the versatile biofoulants, including proteins, blood cells, tissue cells, and bacteria. A self-assembled bioinert interface with universal fouling resistance to general biofoulants via hydrophobic-driven surface PEGylation is presented. Th...
Article
Although PEGylated polyplexes for gene delivery are widespread, there is a need for in-depth investigation on the role of the PEGylation degree on the delivery efficiency of the systems. For this, a low-toxicity series of polymers for gene delivery was designed via Michael addition of poly(ethylene glycol) methyl ether methacrylate (PEGMA) onto bra...
Article
Dengue virus (DENV) has emerged as a major economic concern in developing countries, with 2.5 billion people believed to be at risk. Vascular endothelial cells (ECs) lining the circulatory system from heart to end vessels perform crucial functions in the human body, by aiding gas exchange in lungs, gaseous, nutritional and its waste exchange in all...
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Establishing cultures of human embryonic (ES) and induced pluripotent (iPS) stem cells in xeno-free conditions is essential for producing clinical-grade cells. Development of cell culture biomaterials for human ES and iPS cells is critical for this purpose. We designed several structures of oligopeptide-grafted poly (vinyl alcohol-co-itaconic acid)...
Article
A surface deposition approach enveloping the use of biocompatible trace components and strengthening materials will affect the physicochemical and osseointegration properties of nanocomposite deposited implants. The current work is aimed at the development of functionalized carbon nanotubes (f-CNT)/Pectin (P)/mineralized hydroxyapatite (M-HA) ((f-C...
Article
Helicoverpa armigera Hubner is an important polyphagous agricultural insect pest, highly mobile, with a worldwide distribution. In India, it causes substantial losses to cotton, legume, pulses, cereal oil seed and vegetable crops. Malaria is caused by parasites from the genus Plasmodium that are transmitted by female anopheline mosquitoes. Anophele...