Akhtar Shekafandeh

Shiraz University · Department of Horticulture

The possibility of micropropagation of five selected superior almond genotypes for rootstock purposes were investigated. Shoot tips were subjected to surface disinfection and cultured on Murashige and Skoog (MS) medium containing benzyl adenine (BA) and gibberellic acid (GA3) (2, 3 and 4 mgl⁻¹), indole-3-butyric acid (IBA) 0.1 mgl⁻¹ and thidiazuron...

Wallflower (Erysimum cheiri (L.) Crantz is one of the most important bedding flowers and potted plants in the floriculture industry. Its widespread cultivation is hindered due to self-incompatibility and limited breeding. The present study was conducted to investigate the effects of polyploidy induction on different characteristics of wallflower. S...

Different methods employed in plant tissue culture can provide a valuable source of plants for the horticultural industry and novel germplasm for breeding programs, but the genetic fidelity and lack of somaclonal variation of regenerated plants needs to be verified. In this study, the genetic fidelity of blackberry (Rubus hirtus Waldst. & Kit.) pla...

In this research, the production of superior genotypes via the polyploidy induction method in two citrus species involved the tissue culture of stem nodal segments of Mexican lime and sour orange. The variables included various concentrations of colchicine (0, 125 and 250 mg L⁻¹), which were added to the MS medium, and different durations of exposu...

The fig tree is a well-adapted and promising fruit tree for sustainable production in arid and semi-arid areas worldwide. Recently, Iran's dryland fig orchards have been severely damaged due to prolonged severe and consecutive drought periods. As emphasized in many studies, ploidy manipulated plants have a significantly enhanced drought tolerance....

Grafted fruit trees are made up of two parts, the scion and the rootstock. The rootstocks affect the growth and physiological and biochemical treats of scion. The use of grafting technique in vitro produces healthy plants and allows careful study of rootstock and scion interactions. The objective of this research was to achieve a successful protoco...

Fig tree (Ficus carica L.) is a precious fruit tree in semi-arid and arid areas worldwide which has difficulties in its conventional breeding programs. This study was carried out to make new genotypes with superior features based on the ploidy induction method. Thus, in vitro tetraploidization in two fig cultivars, namely ‘Sabz’ and ‘Torsh’, was su...

The fig (Ficus carica L.), is known as a precious fruit tree for its nutrition and medicinal values, economic importance and for sustainable production in the semi-arid and arid areas. Expanding the cultivation of fig in new vulnerable areas and the breeding programs in fig need a reliable high-efficient system for in vitro morphogenesis to meet fu...

Mexican lime (Citrus aurantifolia Swingle) is the common prevailing rootstock in citriculture industry of southern regions of Iran. This research was planned to evaluate the responding mechanisms of this rootstock under drought stress. So, seedling rootstocks of Mexican lime, sour orange (Citrus aurantium L.), volkameriana (Citrus volkameriana Ten...

In order to evaluate the indices representing the severity of oxidative damage to photosynthesis apparatus under drought, this research was conducted by a greenhouse experiment for four citrus seedling rootstocks. Rootstocks of Mexican lime (Citrus aurantifolia Swingle), sour orange (Citrus aurantium L.), volkameriana (Citrus volkameriana Ten & Pas...

In this study, the physiological response of two cultivars of grapevine (Vitis vinifera cv. Rotabi, and Thompson seedless) was investigated under manganese treatment (manganese sulfate) and water stress conditions. In this regard, foliar application of manganese sulfate (MnSO4) treatment such as: 0, 2 and 4 g/l and water stress (WS) treatment inclu...

The purpose of this study was to evaluate the cell membrane stability and the tolerance of Washington Novell (WN) and Kino Mandarin (KM) grafted onto Mexican Lime (ML) under water stress condition and recovery. A factorial experiment was conducted in the completely randomized design with 4 replications in a plastic greenhouse. Two-years-old graft c...

Effects of water stress deficit were studied on xylem anatomical features and some growth parameters among six olive grafting combinations; Amygdalifolia/Arbequina (Am/Ar), Amygdalifolia/Koroneiki (Am/Ko), Amygdalifolia/Zard (Am/Z), Conservallia/Koroneiki (Co/Ko), Conservallia/Zard (Co/Z) and Conservallia/Arbequina (Co/Ar) of about three-year-old o...

The present study is the first to report somatic embryogenesis (SE) based on a plant regeneration protocol for blackberry. It uses transverse thin cell layer technique (tTCL). Two blackberry genotypes, ‘High prickle’ (Rubus sanctus) and ‘Low prickle’ (Rubus hirtus) were used as explants. The explants were soaked in ascorbic and citric acids (60 mg...

The purpose of this study was to evaluate the biochemical responses to water stress tolerance of two pomegranate cultivars, ‘Rabbab’ and ‘Shishehgap’. After the establishment of rooted stem cuttings of both cultivars under greenhouse conditions, they were treated with four levels of deficit irrigations (100%, 75%, 50% and 25% of field capacity) in...

در اين پژوهش، پرآوري شاخساره از قطعه هاي گرهي تمشک پرخار و تمشک کم‌خار مورد بررسي قرار گرفت. ريزنمونه ها پس از گندزدايي سطحي روي محيط كشت MS محتوی غلظت‌های متفاوت BA و NAA قرار گرفت. در هردو گونه بدون توجه به تغییر میزانNAA، با افزایش میزانBA تعداد شاخه‌های بیشتری در هر جوانه ایجاد شد. بهترین تیمار پرآوری در هر دو گونه 5/0 میلی‌گرم برلیتر NAA و 4 م...

TCLs have served as a simple, but important biotechnological tool in plant science, with several dozen crop species having had tissue culture regeneration protocols developed using TCLs generated from multiple explant sources. The former is the most common, ranging from 100 µm to 1–2 mm in thickness (da Silva, 2004).

Blackberry, an aggregate fruit of Rubus species subgenus Rubus in the Rosaceae family, has long been a favorite wild fruit. The success of Micropropagation and regeneration is dependent on genotype, the composition of the culture medium and the presence of appropriate combinations and concentrations of hormones in the culture media.

TCLs have served as a simple, but important biotechnological tool in plant science, with several dozen crop species having had tissue culture regeneration protocols developed using TCLs generated from multiple explant sources. The former is the most common, ranging from 100 µm to 1–2 mm in thickness (da Silva, 2004).

Adventitious shoot regeneration from mature cotyledons of GF677 rootstock (Prunus persica × Prunus amygdalus) was achieved in vitro. Thidiazuron (N-phenyl-N-1,2,3-thidiazol-5-yl-urea; TDZ) at 32 µM gave the highest percentage of cotyledons forming adventitious shoots (68.8%) and the highest number of shoots per cotyledon (4.8) on Quoirin and Lepoiv...

The improvement of loquat seed germination, seedling growth was investigated using seed priming technique.

The improvement of loquat seed germination, seedling growth was investigated using seed priming technique.

تنظیم کننده های رشد موثر در پرآوری دو گونه بومی پرخار و کم خار تمشک ایران بررسی شده است.

In this research, in vitro propagation of three endangered and endemic fig genotypes named ‘Bargchenari’, ‘Dehdez’ and ‘Runu’ were investigated. For shoots proliferation, nodal explants on Murashige and Skoog (MS) medium were treated with different concentrations of growth regulators. Rooting of shoots was initiated by the use of MS and MS/2 media...

Callus production, somatic embryogenesis and plant regeneration were achieved from juice vesicles of Mexican lime. First, three types of culture media, Murashige and Skoog (MS), Murashige and Tucker (MT) and Grosser and Gmitter (H+H) and different levels of 2,4-Dicholorophenoxy acetic acid (2,4-D) were used to study their effects on production and...

In order to evaluate the influence of drought stress on two fig cultivars ('Siah' and 'Sabz'), uniform rooted cuttings of both cultivars were potted in 7 L pots filled with soil mixture. The treatments consisted of 4 drought intervals of 4, 8, 12 and 16 days were applied to attain osmotic potentials of-0.6,-1.15,-1.5 and-1.75 bars, respectively. Th...

A major portion of the Iranian fig (Ficus carica) industry is located in high-salinity regions, and salinity is an important limiting factor in the production of this fruit. The present study was conducted to investigate the changes of leaf antioxidant-enzyme activity, proline and total protein content in two fig cultivars with two leaf shapes: 'An...

A major portion of the Iranian fig (Ficus carica) industry is located in high-salinity regions, and salinity is an important limiting factor in the production of this fruit. The present study was conducted to investigate the changes of leaf antioxidant-enzyme activity, proline and total protein content in two fig cultivars with two leaf shapes: 'An...

In this research, the effects of different salt concentration (0, 80, 120 and 180 mM NaCl) on physiological and biochemical changes of two fig cultivars ‘Anjir Sabz’ and ‘Shah Anjir’ were evaluated via in vitro culture. A factorial experiment of 4(levels of salt) ×2 (cultivars) in a completely randomized design with 4 replicates and 3 seedlings in...

In this research, the changes in plant growth, lipid peroxidation, protein content and activities of antioxidant enzymes in loquat seedlings subjected to NaCl stress and 24-epibrassinolide (24-EBL) application were investigated. Plants were treated with 5 levels of salt (0.5, 2, 4, 6 and 8 dS m-1) and 4 levels of 24-epibrassinolide solution (0, 0.2...

Summery This study was carried out to investigate the role of 24-epibrassinolide (24-EBL) in inducing loquat plant salt tolerance. Plants were treated with five levels of salt, so that the electrical conductivity (EC) of 0.5 (control), 2, 4, 6 and 8 dS m -1 was established in pots. Then, the plants were sprayed with different concentrations of 24-E...

Two grafted cultivars of Z. spina-christi ('Danehgird' and 'Danehboland' were grafted on wild seedlings) and non-grafted seedlings were grown in soil and perlite mixture (1:1 v/v) and treated with 0 (0 dS m(-1)), 3.2 (5 dS m(-1)), 6.4 (10 dS m(-1)) and 12.8 (20 dS m(-1)) g l(-1) NaCl. After 16 weeks, salt stress resulted in a substantial decrease i...

This study was conducted to evaluate the effect of different types and concentrations of priming solution on seed germination and seedling performance of spina-christi. The seeds were primed in solutions of sodium chloride of 0, 4, 8, 12 and 16 dS/m, zinc sulfate of 0, 4, 8, 12 and 16 dS/m (osmopriming), distilled water (D.W.) and distilled water a...

Narcissus pseudonarcissus cv. Dutch Master bulbs treated with soaking in solution of NPK, 20:20:20 before planting and then planted in pots containing soil mixture. Soaking was done in concentrations 2, 4, 6, 8, 10 gl-1 and in times 20, 40 and 60 min. Small Narcissus bulbs (10 -11 g of weight and 9-10 cm of circumference) was used for this experime...

This study was carried out on olive trees cv. Amygdalifolia, to investigate the effect of spraying potassium nitrate (KNO3) and zinc sulfate (ZnSO4) on the fruit weight, dimension and volume. The plants were sprayed with 0, 0.5, 1.0 and 1.5% KNO3 and 0, 0.25, 0.50 and 0.75% ZnSO4 and their combinations in August about halfway through the fruit grow...

This study was conducted on ten-year-old ‘Shengeh’ olive trees at the Kazerun Olive Research Station, Iran to investigate the effect of gibberellic acid (GA3) and zinc sulfate (ZnSO4) sprays on fruit yield and oil production. A factorial experiment (4 × 4 × 4) was arranged in a randomized complete block design. The plants were sprayed with 0, 15, 3...

Olive trees (Olea europaea L. cv. Shengeh) were treated with gibberellic acid (GA 3) at 0, 15, 30 and 45 ppm and zinc sulphate at 0, 0.25, 0.5 and 0.75% when fruit were at 70% of their final size in experiments carried out in 2007 season. The effect of GA 3 and ZnSO 4 treatments on yield and some variables related to fruit characteristics (fruit, p...

In this research, a factorial 4×3×4 with 4 replications in Completely Randomized design was used to study the effects of different auxin treatments (4 levels), pH(2 levels) and cultivars (3 levels) on regeneration and rooting of in vitro shoot tips excised from shoots cultured on media supplemented with 1.5-2 mg/l BA. The results showed both auxins...

An effective procedure of pomegranate (P. granatum L. cv. Rabbab) micropropagation is reported. Nodal segments were used as explants. The results showed that a combination of alcohol, warm water and sodium hypochlorite was needed to control fungal and bacterial contaminations. A comparison between three types of cytokinin (BA, TDZ and kin) on shoot...

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In this study, hot-water and cold treatments were used for eradication of explant contamination, and also the effect of plant growth regulators on shoot proliferation was evaluated. The explants were nodal segments of a late flowering almond cultivar 'Sharood 7'. Experiments were carried out in a complete randomized design with 25 replications. All...

In order to investigation of effective optimum condition effecting callus induction, close floral bud of olive conservolea cultivar harvested. After disinfect ion of anther explants cultured on the full MS and modified MS medium with different concentrations of TDZ, NA A, 2,4-D and 2ip. Cultured anthers in the full M S medium a little swallowed and...

Caper (Capparis spinosa L.) is an adaptable plant to rangelands which is also planted for use as a condiment, for forage and for prevention of soil erosion. Seeds of this species have poor germination in natural conditions. We subjected the seeds to different dormancy breaking treatments (sulfuric acid for 20, 30 and 40 min., soaking in warm water,...

In this research, the rate of shoot proliferation from different nodes in different positions on shoot [uppermost node (N<SUB>1</SUB>), the second node (N<SUB>2</SUB>) and the third node (N<SUB>3</SUB>)] from two mature guava cultivars, white fleshed Local-1 and red fleshed Local-2 on different culture media (MS and WPM) with different BA concentra...

Nodal segments of wild Iranian myrtle was used to study the effects of two carbohydrate sources on shoot proliferation and to determine residual effect of different growth regulators and carbohydrate sources on in and ex vitro rooting. Half strength Murashige and Skoog (MS) medium was used for both shoot proliferation and rooting. The highest shoot...

Salt tolerance is a very needed potentiality in cereal crops, since it would allow to extend cultures to dryer areas. The biotechnological approach and more precisely, in vitro tissue culture, has been experimented, using gametophytic (either male or female) tissues as source of variability. In cereals, albinism is frequently associated to androgen...

Salt tolerance is a very needed potentiality in cereal crops, since it would allow to extend cultures to dryer areas. The biotechnological approach and more precisely, in vitro tissue culture, has been experimented, using gametophytic (either male or female) tissues as source of variability. In cereals, albinism is frequently associated to androgen...

In Triticum durum Defs., gynogenesis was investigated on six genotypes, Cocorit, Isly, Jori, Oued Zénati, Sarif, issued from Morocco,and Cham1 from Syria. The experiments described here were made during two seasons (spring and summer) in which batches of 1,036 and 3,750 unpollinated ovaries were used. A 4 °C pretreatment was applied, during 7, 11 o...

Rapid clonal propagation of a local cultivar of myrtle (Myrtus communis L.) was achieved in vitro using tips excised from vegetative shoots of outdoor-grown plants. Optimum shoot proliferation was obtained on a medium containing half-strength Murashige and Skoog (1962) (MS) salts plus (in mg l−1): nicotinic acid, 0.5; pyridoxine HCl, 0.5; thiamin H...