Akhtar Nadhman

Akhtar Nadhman
International Islamic University, Islamabad | IIUI · Sulaiman Bin Abdullah Aba Al Khail Centre for Interdisciplinary Research in Basic Sciences

PhD

About

57
Publications
26,849
Reads
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1,792
Citations
Additional affiliations
March 2016 - May 2016
International Islamic University, Islamabad
Position
  • Professor (Assistant)
November 2013 - April 2014
Harvard University
Position
  • Pre-doctoral Research Fellow
October 2013 - May 2014
Massachusetts Institute of Technology
Position
  • Pre-doctoral Research Fellow

Publications

Publications (57)
Article
Full-text available
This study is aimed at the chemical synthesis of light-activated cobalt-doped zinc oxide and its further doping on reduced graphene oxide (RGO) and assessment of its antibacterial activity on antibiotic-resistant waterborne pathogens including Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumonia, and Pseudomonas aeruginosa. The synthe...
Article
Full-text available
The callus cultures of Fagonia indica could prove as factories for the production of important phytochemicals when triggered through different types of stress. In this study, we initiated callus cultures from healthy stem explants in the presence of iron-doped zinc oxide nanoparticles (Fe-ZnO-NPs). We performed experiments with the callus cultures...
Preprint
Full-text available
Fagonia indica is an important medicinal plant species used traditionally against a variety of diseases. In this study, we initiated callus cultures from healthy stem explants. We observed maximum callus induction frequency (88%) on MS media supplemented with Thidiazuron (1.0 mg/mL). We also examined the callus cultures to determine the impact of i...
Article
There are numerous trials underway to find treatment for the COVID-19 through testing vaccines as well as existing drugs. Apart from the many synthetic chemical compounds, plant-based compounds could provide an array of important suitable candidates for testing against the virus. Studies have confirmed the role of many plants against respiratory vi...
Article
Full-text available
Physicochemical parameters include pH, temperature, the concentration of the AgNO3, ratio of reactants, agitation and incubation period that act synergistically and provide a steering force to modulate the biogenesis of nanoparticles by influencing the molecular dynamics, reaction kinetics, protein conformations, and catalysis. The current study in...
Article
Flower like zinc oxide nanoparticles have been synthesized and the effects of their aqueous suspensions at 200–1000 mg/L on seed germination and seedlings of Triticum aestivum have been investigated. When treated with suspensions of zinc oxide nanoparticles at concentrations of 200–1000 mg/L, germination was reduced to 45% and photosynthetic pigmen...
Preprint
There are numerous trials underway to find treatment for the COVID-19 through testing vaccines as well as existing drugs. Apart from the many synthetic chemical compounds, plant-based compounds could provide an array of important suitable candidates for testing against the virus. Studies have confirmed the role of many plants against respiratory vi...
Article
Full-text available
The present study involves the Phyto-synthesis of the colloidal silver nanoparticles (AgNPs) and their applications to biologically control plant bacterial pathogens. The synthesis of AgNPs was monitored by measuring the absorbance and a characteristic surface plasmon resonance (SPR) band was observed at 450 nm. The different reaction conditions su...
Article
A facile, green synthesis of selenium doped zinc oxide nano-antibiotic (Se-ZnO-NAB) using the Curcuma longa extract is reported to combat the increased emergence of methicillin-resistant Staphylococcus aureus (MRSA). The developed Se-nO-NAB were characterized for their physicochemical parameters and extensively evaluated for their toxicological pot...
Article
Full-text available
The present investigation was aimed to phytosynthesize silver nanoparticles (AgNPs) by utilizing aqueous extract of Mentha longifolia branches to reduce mass Ag into Ag° and evaluation of their potential to treat HCT116 colon cancer, Leishmanial, and bacterial cells. A characteristic Surface Plasmon Resonance (SPR) band to confirm synthesis was obs...
Article
Full-text available
The present study deals with the ecofriendly one-pot synthesis and stabilization of silver nanoparticles (AgNPs) by using aqueous extract of Mentha longifolia branches. Spectrophotometric analysis of different ratios of reactants revealed that a 1 to 9 ratio of plant extract and silver salt solution respectively is the most suitable proportion for...
Article
Full-text available
The current study aims to assess the aqueous extract of Pinus wallichiana stem for the synthesis of small spherical-shaped (10–30 nm) silver nanoparticles (AgNPs) and their in-vitro and in-vivo biomedical applications. The biosynthesized AgNPs were nonmutagenic and safe at all test doses as per Ames and acute toxicity assay (20, 40, 60, and 80 mg/k...
Article
Full-text available
The present study was aimed to use an aqueous extract of Mentha arvensis L. to bio-fabricate silver nanoparticles (AgNPs) and evaluation of the potential of non-oxidative nanoparticles in photodynamic treatment of pathogens. Spectrophotometric analysis of colloidal AgNPs represented a characteristic SPR band at 485 nm of the light wavelength. Nanop...
Article
Full-text available
Aim: To investigate the photodynamic therapeutic potential of ferromagnetic iron oxide nanorods (FIONs), using Trigonella foenum-graecum as a reducing agent, against Leishmania tropica. Materials & methods: FIONs were characterized using ultraviolet visible spectroscopy, x-ray diffraction and scanning electron microscopy. Results: FIONs showed exce...
Article
Background Liver cancer, a devastating cancer with increasing incidence and mortality rates worldwide. Plants possess numerous therapeutic properties, therefore the search for novel, naturally occurring cytotoxic compounds is urgently needed. Methods The anticancer activity of plant extracts and isolated compounds from Anchusa arvensis (A. arvensi...
Preprint
The current study was carried out to compare the antileishmanial potential of the characterized chemically and plant mediated (Trigonella foenum-graecum) FeO-NPs (FeO-NPs) against L.tropica KWH23. The promastigotes and amastigotes mortality, ROS generation, and biocompatibility assessment were estimated in time-dose dependent manner by PDT. LED exp...
Article
The worldwide focus on research in the field of green nanotechnology has resulted in the environmentally and biologically safe applications of a diversity of nanomaterials. Nanotechnology, in general, implies the production of nanoparticles having different but regular shapes, sizes, and properties. A lot of studies have been conducted on the synth...
Article
Full-text available
This study was aimed to develop a self‐nanoemulsifying drug delivery system (SNEDDS) for amphotericin B (AmB) potential use in leishmaniasis through topical and oral routes. Two formulations, formulation A and formulation B (FA and FB) of AmB loaded SNEDDS were developed by mixing their excipients through vortex and sonication. The SNEDDS formulati...
Chapter
Nanotechnology is a multipurpose field that has diverse applications in almost every field of science. Nanotechnology provides substantial opportunities for the scientists working in Plant sciences and Agricultural biotechnology to develop and adopt new methods and tools for improvement of commercially important plants and crops. Nanotechnology mig...
Article
The current study was aimed to evaluate the anti-leishmanial potentials of β-sitosterol isolated from Ifloga spicata. The anti-leishmanial potential of β-sitosterol is well documented against Leishmania donovani and Leishmania amazonensis but unexplored against Leishmania tropica. Structure of the compound was elucidated by FT-IR, mass spectrometry...
Article
Full-text available
This study aimed to appraise the anti-leishmanial potentials of benzoic acid derivatives, including methyl 3,4-dihydroxybenzoate (compound 1) and octadecyl benzoate (compound 2), isolated from the ethnomedicinally important plant Ifloga spicata (I. spicata). Chemical structures were elucidated via FT-IR, mass spectrometry, and multinuclear (1H and...
Article
Background: Leishmaniasis, which is classified by the World Health Organization (WHO) as one of the neglected tropical diseases (NTDS) faces several challenges in terms of successful chemotherapy and novel drug developments. Objective: The aim of the present study was to develop a Self-Emulsifying Drug Delivery System (SEDDS) for the hydrophobic...
Article
Full-text available
Despite of the remarkable cytotoxic and imaging potential of ultra-small metal nanoclusters, their toxicity-free and targeted delivery to cancerous cells remains a substantial challenge that hinders their clinical applications. In this study, a polymeric scaffold was first synthesized by grafting folic acid and thiol groups to chitosan (CS) for can...
Article
The current study is based on bio-inspired synthesis of visible light responsive ferromagnetic iron oxide nanoparticles (FIONs) using Trigonella foenum-graecum plant and its efficacy as anti-leishmanial agents. Preliminary findings about time- and dose-dependent effectiveness of FIONs on different biological parameters of Leishmania tropica have be...
Article
Various types of nanoparticles (NPs) have been used in delivering anticancer drugs to the site of action. This area has become more attractive in recent years due to optimal size and negligible undesirable side effects caused by the NPs. The focus of this review is to explore various types of NPs and their surface/chemical modifications as well as...
Article
Full-text available
The aim of this study was to evaluate mannose-anchored thiolated chitosan (MTC) based nanocarriers (NCs) for enhanced permeability, improved oral bioavailability and anti-parasitic potential of amphotericin B (AmB). Transgenic Leishmania donovani parasites expressing red fluorescent protein DsRed2 and imaging-flow cytometry was used to investigate...
Article
Full-text available
Polymeric nanomaterials, hybridized with lipid components e.g. phosphocholine or fatty acids, are currently being explored for efficient nano-platforms for hydrophobic drugs. However, their toxicology and toxicokinetics needs to be established before enabling their clinical potential. The aim of this study was to investigate the toxicological profi...
Article
Intramacrophage parasite 'Leishmania' has developed various mechanisms for proficient uptake into macrophages and phagosome regulation to avoid macrophage's oxidative burst induced by peroxide, hydroxyl radical, hypochlorous acid and peroxynitrite production. One major barrier for impairing the accession of old fashioned anti-Leishmanial drugs is i...
Article
Full-text available
Essential oils isolated from leaves, barriers and from their mixture of Juniper (Juniperus excelsa M.Bieb) growing in Ziarat district. Essential oils were obtained by Clevenger-type apparatus, and components identified by gas chromatography mass spectroscopy (GC-MS). A total 27 components were identified which were inconsistent in their composition...
Article
Full-text available
Human’s quest for innovation, finding solutions of problems, and upgrading the industrial yield with energy efficient and cost-effective materials has opened the avenues of nanotechnology. Among a variety of nanoparticles, zinc oxide nanoparticles (ZnO) have advantages because of the extraordinary physical and chemical properties. It is one of the...
Article
Folate grafted and thiolated chitosan was synthesized and wrapped on the surface of mixed phosphatidylcholine based nanoliposomes (NLs) to improve the oral absorption and targeted pharmacological activity of anti-cancer drugs against breast cancer. In this study, chitosan derived thiomer, having intrinsic property of P-glycoprotein (P-gp) efflux pu...
Article
In pursuit of safe drug candidates for the treatment of parasitic diseases like leishmaniasis, a series of heteroleptic pentavalent antimonials of the type [R3Sb(O2CR′)2] (1–9) have been synthesized and characterized using elemental analysis (CHN), Fourier transform infrared spectroscopy and multinuclear (1H and 13C) NMR spectroscopy. The carboxyla...
Article
Full-text available
Leishmaniasis is a clinical manifestation caused by the parasites of the genus Leishmania. Plants are reservoirs of bioactive compounds, which are known to be chemically balanced, effective and least injurious as compared with synthetic medicines. The current resistance and the toxic effects of the available drugs have brought the trend to assess t...
Article
Hirudin is clinically an important drug used for the treatment of cardiac diseases, but has never been elucidated for anti-leishmanial potential. The present study was designed to determine the therapeutic utility of hirudin against leishmaniasis. Binding affinities of 28 potent proteinase inhibitors were screened computationally against leishmanol...
Article
There is a growing interest to develop microfluidic bioreactors and organ-on-chip platforms with integrated sensors to monitor their physicochemical properties and to maintain a well-controlled microenvironment for cultured organoids. Conventional sensing devices cannot be easily integrated with microfluidic organ-on-chip systems with low-volume bi...
Article
In the quest for new drug candidates for the safe treatment of parasitic diseases like leishmaniasis, a series of heteroleptic pentavalent antimonials of the type [SbR3(OOCR′)2] were synthesized and characterized using elemental analysis, Fourier transform infrared spectroscopy and multinuclear (1H and 13C) NMR spectroscopy. The carboxylate moietie...
Article
Full-text available
Lipid and protein oxidation are well-known manifestations of free radical activity and oxidative stress. The current study investigated extermination of Leishmania tropica promastigotes induced by lipid and protein oxidation with reactive oxygen species produced by PEGylated metal-based nanoparticles. The synthesized photodynamic therapy-based dope...
Article
Full-text available
Background: Enterococci have emerged as more virulent and multidrug-resistant in community and hospital settings. The emergence of vancomycin resistant enterococci (VRE) in hospitals has posed a serious threat to public health. The widespread use of antibiotics to treat VRE infections has resulted in the development of resistant forms of these org...
Article
Full-text available
A series of ferrocenyl pentavalent antimonials (1-8) were synthesized and characterized by elemental analysis, FT-IR, and multinuclear ((1) H and (13) C) NMR spectroscopy. These antimonials were evaluated for their antileishmanial potential against Leishmania tropica KWH23, and by biocompatibility and membrane permeability assays. Moreover, mechani...
Article
Full-text available
Synthesis of silver nanoparticles by plants and plant extracts (green synthesis) has been developed into an important innovative biotechnology, especially in the application of such particles in the control of pathogenic bacteria. This is a safer technology, biologically and environmentally, than synthesis of silver nanoparticles by chemical or phy...
Article
Full-text available
Human beings suffer from several infectious agents such as viruses, bacteria, and protozoans. Recently, there has been a great interest in developing biocompatible nanostruc-tures to deal with infectious agents. This study investigated benign ZnCuO nanostructures that were visible-light-responsive due to the resident copper in the lattice. The nano...
Article
Full-text available
Recently, the authors reported newly synthesised polyethylene glycol (PEG)ylated silver (9%)-doped zinc oxide nanoparticle (doped semiconductor nanoparticle (DSN)) which has high potency for killing Leishmania tropica by producing reactive oxygen species on exposure to sunlight. The current report is focused on Leishmania DNA interaction and damage...
Article
Full-text available
In the present study, the efficacy of antibiotic-nanoparticle conjugates against the clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible Staphylococcus aureus (MSSA) were invesitgated. MRSA and MSSA were isolated from ICU patients and successfully confirmed by molecular methods. Antibiotic disc diffus...
Article
Full-text available
We have designed and fabricated a miniature microscope from off-the-shelf components and a webcam, with built-in fluorescence capability for biomedical applications. The mini-microscope was able to detect both biochemical parameters, such as cell/tissue viability (e.g. live/dead assay), and biophysical properties of the microenvironment such as oxy...
Article
Full-text available
The degradation ability of Aspergillus sp. strain NA-25 was determined against poly (3-hydroxybutyrate) (PHB), since it was previously tested against co-polymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate). PHB depolymerase enzymes were produced from strain NA-25 at 45 o C and pH 7.0 after 96h of incubation. The enzymes were purified to homogeneity...
Article
Full-text available
The use of photoactive nanoparticles (NPs) such as zinc oxide (ZnO) and its nanocomposites has become a promising anticancer strategy. However, ZnO has a low photocatalytic decomposition rate and the incorporation of metal ions such as silver (Ag) improves their activity. Here different formulations of ZnO:Ag (1, 3, 5, 10, 20 and 30% Ag) were synth...
Article
Full-text available
We describe daylight responsive silver (Ag) doped semiconductor nanoparticles of zinc oxide (DSNs) for the Photodynamic Therapy (PDT) against Leishmania. The developed materials were characterized by X-Ray Diffraction analysis (XRD), Rutherford Back Scattering (RBS), Diffused Reflectance Spectroscopy (DRS) and band-gap analysis. The Ag doped semico...
Article
Full-text available
Leishmaniasis is an important parasitic problem and is in focus for development of new drugs all over the world. Objective of the present study was to evaluate phytochemical, toxicity, and antileishmanial potential of Jurinea dolomiaea, Asparagus gracilis, Sida cordata, and Stellaria media collected from different areas of Pakistan. Dry powder of p...
Article
Full-text available
A poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) degrading bacterial strain designated as AF-111 was isolated from sewage sludge sample. The bacterium was identified by 16S rRNA gene sequencing. The results revealed that strain AF-111 showed 99 % similarity with Streptomyces althioticus strain NRRL B-3981 and designated as Streptomyces sp. str...
Article
Full-text available
Leishmaniasis is a complex of diseases with numerous clinical manifestations for instance harshness from skin lesions to severe disfigurement and chronic systemic infection in the liver and spleen. So far, the most classical leishmaniasis therapy, despite its documented toxicities, remains pentavalent antimonial compounds. The arvailable therapeuti...
Article
Full-text available
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) degrading thermophilic fungus was isolated from soil sample collected from waste disposal site, Islamabad, Pakistan. It was able to grow efficiently on a medium containing PHBV as a sole source of carbon and has been identified as Aspergillus sp. NA-25 by 18S rRNA. Using 9% of inoculum maximum pro...

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