Akhmadzhan Makhsumkhanov

• Doctor of Philosophy
• Senior Researcher at Uzbekistan Academy of Sciences

This article provides a study of the molecular, physiological, biochemical and morphological features of R-, S- and M-dissociants of Rhodococcus ruber - 8/4/1. The sequence similarity of the genome fragments of R- and S-dissociant forms encoded by 16S rRNA was demonstrated. MALDI spectra were obtained for the R. ruber-8/4/1 strain and its dissocian...

In this review, the data on the use of bacteria of the genus Rhodococcus in the biocatalytic synthesis of amides and other compounds by biotransformation of the corresponding precursors have been presented. An adaptation ability of these bacteria to various extreme environmental conditions, the synthesis of different enzymes and the participation i...

This paper describes an express method, which has been developed for the primary screening of the acid-forming mycelial fungi belonged to Aspergillus and Penicillium genus on selective solid nutrient (SSN) medium. It was established that eight strains of mycelial fungi had the highest acid-forming ability on SSN medium among the isolated 63 natural...

For isolation and purification of nitrile hydratase from biomass of Rhodococcus ruber- 8/4/1 strain, cells grown in peptone containing (PS) medium were centrifuged,collected, disintegrated by ultrasound and cell lysate have been obtained. Cell lysate was applied for gel-filtration on TSK Gel HW 55F and Sephacryl S-100 HR column. Gel filtration on S...

https://doi.org/10.5281/zenodo.8368209 Abstract. In order to select bacteria, producing thermostable α-amylase, 62 isolates isolated from soil samples of Kashkadarya, Samarkand and Tashkent regions have been screened for starch hydrolysis properties. As a result, 8 bacterial strains belonging to the genus Bacillus were selected as potential α-amy...

https://doi.org/10.5281/zenodo.8372639 Аbstract.The wide application and development of effective technologies for the production of industrial enzymes with the help of microorganisms attracts a growing number of researchers in this field. The aim of the present work was to screen bacteria of the genus Bacillus from the local collection of the Ins...

This article presents the results of a study on the possibility of intensifying the process of destruction of oil and oil products by a balanced methanogenic association of microorganisms (obtained after fermentation under thermophilic conditions) (BTMAM) by introducing into them a strain of the bacterium Rhodococcus sp. HN4. The amount of inoculum...

21 века. Их значение на мировом рынке растет: сегодня рынок биоПАВ оценивается в 2374 млн долл. США, и по прогнозам в течение 2022-2027 гг. среднегодовой темп роста составит более 5,5 % [1, 2]. В настоящее время проводятся много-численные исследования по выделению биоПАВ из различных микроорганиз-мов, однако бактерии рода Acinetobacter заслуживают...

In the experiment, bacteria were isolated from soil contaminated with oil residues and identified in a MALDI TOF mass spectrometer. The characteristics of the formation of biological surfactants were studied in the separated samples according to the emulsification index. The growth of the samples in nutrient media with different carbohydrate source...

The research on studies of the various gamma-irradiation doses effect for survival and ability of mutant strains to synthesize biosurfactants has been conducted. It was shown that gamma-irradiation with inducing dose in 2-3 kGr for bacteria of the genus Rhodococcus, more than 1.4 times increasing of biosurfactant biosynthesis and surviving of cells...

In order to isolate active strains of microorganisms, producers of biosurfactants, oil sludge and smeared wastewater samples of the Bukhara refinery plant and oil-gas wells of Kashkadarya region were used. As a result, 29 strains of bacteria belonging to the genus Bacillus have been isolated in pure culture. The totality of data obtained during the...

Partially purified enzyme preparation with specific activities of 153.7 U/mg for α-amylase and 0.15 U/mg for protease was produced by selective adsorption on starch. Enzymes were purified until homogeneous electrophoretically by gel-filtration over HW-55 TSK-gel with specific activities of 245 U/mg for α-amylase and 1.44 U/mg for protease. The opti...

Wild-type cytochrome P450 monooxygenase from Bacillus megaterium (P450 BM-3) has a low hydroxylation activity for beta-ionone (<1 min(-1)). Substitution of phenylalanine by valine at position 87 led to a more than 100-fold increase in beta-ionone hydroxylation activity (115 min(-1)). Enzyme activity could be further increased by both site-directed...

Cultivation of the fungus Penicillium melinii UzLM-4 on a Raistrick's medium of our own modification made it possible to increase the biosynthesis of lipases three to four times. The following conditions ensured a high rate of synthesis of the extracellular lipase: age of the inoculum, 15 days; concentration of the inoculum, 15 x 10(6) conidia per...

Cultivation of the fungus Penicillium melinii UzLM-4 on a Raistrick's medium of our own modification made it possible to increase the biosynthesis of lipases by three to four times. The following conditions ensured a high rate of synthesis of the extracellular lipase: age of the inoculum, 15 days; concentration of the inoculum, 15 106 conidia per 1...