Akhil Srivastava

Akhil Srivastava
University of Missouri | Mizzou · Pathology & Anatomical Sciences

Ph.D.

About

43
Publications
6,234
Reads
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965
Citations
Introduction
Akhil Srivastava currently works at the Stephenson Cancer Center, University of Oklahoma Health Sciences Center. Their current projects are nanomedicine and Immuno-oncology
Additional affiliations
January 2019 - February 2019
University of Oklahoma Health Sciences Center
Position
  • Professor (Assistant)
January 2019 - February 2019
University of Oklahoma Health Sciences Center
Position
  • Professor (Assistant)
January 2009 - September 2013
University of Hawaiʻi at Mānoa
Position
  • Research Assistant

Publications

Publications (43)
Article
Full-text available
Extracellular vesicles (EVs), is the umbrella term used for different types of vesicles produced by the cells, among which exosomes form the largest group. Exosomes perform intercellular communication by carrying several biologics from donor or parental cells and delivering them to recipient cells. Their unique cargo-carrying capacity has recently...
Article
Diabetes mellitus (DM), is the most common metabolic disease and is characterized by sustained hyperglycemia. Accumulating evidences supports a strong association between DM and numerous lung diseases including chronic obstructive pulmonary disease (COPD), fibrosis, and lung cancer (LC). The global incidence of DM-associated lung disorders is risin...
Article
Full-text available
Exosomes are involved in cell-to-cell communication and play a crucial role in cellular physiology. The role of exosomes in cancer has been widely explored. Tumor cells have evolved and adapted to evade the immune response. The study of the immune system’s modulations in favor of rogue tumor cells led to the development of a novel immunotherapeutic...
Article
Full-text available
Background: Treatment of metastatic melanoma possesses challenges due to drug resistance and metastases. Recent advances in targeted therapy and immunotherapy have shown clinical benefits in melanoma patients with increased survival. However, a subset of patients who initially respond to targeted therapy relapse and succumb to the disease. Therefo...
Article
Exosomes are small membranous vesicles implicated in intercellular signalling. Through their uncanny ability to carry and deliver donor cellular cargo (biomolecules) to target cells, they exert a profound effect on the regular functioning of healthy cells and play a significant role in pathogenesis and progression of several diseases, including can...
Article
Full-text available
Under the broader category of extracellular vesicles (EVs), exosomes are now well recognized for their contribution and potential for biomedical research. During the last ten years, numerous technologies for purification and characterization of EVs have been developed. This enhanced knowledge has resulted in the development of novel applications of...
Article
Full-text available
Aberrant expression of GLI1 is responsible for aggressive tumor behavior and survival due to its effects on the DNA damage response (DDR). We investigated whether interleukin (IL)-24, a tumor suppressor, inhibits GLI1 and the associated DDR pathway in human NSCLCs. IL-24 treatment reduces mRNA and protein expression of GLI1 in lung tumor cells, but...
Article
Exosomes have great potential to serve as a source of diagnostic and prognostic biomarkers for endometrial cancer (EC). Urine-derived exosomes from patients with EC and patients with symptoms of EC, but without established EC, were used to evaluate a unique miRNA expression profile. Of the 84 miRNA studied, 57 were amplified in qPCR, suggesting the...
Article
Full-text available
Extensive research in genetics and genomics has revealed that lung cancer is a physiologically complex and genetically heterogeneous disease. Although molecular targets that can yield favorable response have been identified, those targets cannot be exploited due to the lack of suitable drug carriers. Furthermore, lung cancer often is diagnosed at a...
Article
Co-administration of functionally distinct anti-cancer agents has emerged as an efficient strategy in lung cancer treatment. However, a specially designed drug delivery system is required to co-encapsulate functionally different agents, such as a combination of siRNA and chemotherapy, for targeted delivery. We developed a folic acid (FA) -conjugate...
Article
Full-text available
The Hippo pathway is an evolutionarily conserved signaling pathway that regulates proliferation and apoptosis to control organ size during developmental growth. Yes-associated protein 1 (YAP1), the terminal effector of the Hippo pathway, is a transcriptional co-activator and a potent growth promoter that has emerged as a critical oncogene. Overexpr...
Article
Full-text available
Selective downregulation of the human antigen R (HuR) protein by siRNA may provide a powerful approach for treating lung cancer. To this end, we investigated the efficacy of transferrin receptor-targeted liposomal nanoparticle-based HuR siRNA (HuR-TfNP) therapy and compared to control siRNA (C)-TfNP therapy both, in vitro and in vivo using lung can...
Chapter
Effective and safe delivery of anticancer agents is among the major challenges in cancer therapy. The majority of anticancer agents are toxic to normal cells, have poor bioavailability, and lack in vivo stability. Recent advancements in nanotechnology provide safe and efficient drug delivery systems for successful delivery of anticancer agents via...
Article
Full-text available
Successful chemotherapeutic intervention for management of lung cancer requires an efficient drug delivery system. Gold nanoparticles (GNPs) can incorporate various therapeutics; however, GNPs have limitations as drug carriers. Nano-sized cellular vesicles like exosomes (Exo) can ferry GNP-therapeutic complexes without causing any particle aggregat...
Article
Background: High mobility group A1 (HMGA1), a member of the non-histone chromosomal proteins and commonly referred to as architectural transcription factor, regulates transcription of various genes involved in cell growth and survival. Overexpression of HMGA1 has been shown to be associated with tumor progression and metastasis in several cancers,...
Article
The bottleneck in current vector-based cancer therapy is the targeted and controlled release of therapeutics in tumors. Exosomes are submicron-sized vesicles that are secreted by all cell types and are involved in communication and transportation of materials between cells. Analogous in size and function to synthetic nanoparticles, exosomes offer m...
Article
The bottleneck in current vector-based cancer therapy is the targeted and controlled release of therapeutics in tumors. Exosomes are submicron-sized vesicles that are secreted by all cell types and are involved in communication and transportation of materials between cells. Analogous in size and function to synthetic nanoparticles, exosomes offer m...
Article
Full-text available
Alternaria brassicicola causes black spot disease of Brassica species. The functional importance of pectin digestion enzymes and unidentified phytotoxins in fungal pathogenesis has been suspected but not verified in A. brassicicola. The fungal transcription factor AbPf2 is essential for pathogenicity and induces 106 genes during early pathogenesis,...
Article
Predatory behavior of a nematode is usually determined through gut content observation or prey delimitation counts. In this experiment, Mononchus and Neoactinolaimus predation of Rotylenchulus reniformis or Meloidogyne incognita was determined using a PCR-based nematode gut content analysis. Soil samples naturally infested with Mononchus were place...
Article
Full-text available
Exosomes are 30 - 100 nm bodies secreted from almost all types of cells into the extracellular spaces. They enclose in their lumen active genetic information in the form of messenger RNA (mRNA), micro RNA (miRNA), DNA and active peptides that are representative of the parental cell and can be isolated from different body fluids. Exosomes can partic...
Article
Full-text available
Interleukin (IL)-24, a novel tumor suppressor/cytokine exhibits antitumor activity against a broad-spectrum of human cancer cells. In a recent study, we showed that IL-24 inhibited AKT in lung cancer cells. However, the molecular mechanism of AKT inhibition by IL-24 remains elusive. The high mobility group (HMG) A1 a member of the non-histone chrom...
Patent
Full-text available
A computer-implemented method for analyzing malware may include: 1) identifying a set of malware samples, 2) identifying, for each malware sample in the set of malware samples, a set of static strings present in the malware sample, and then 3) clustering the set of malware samples based on the set of static strings present in each malware sample. V...
Chapter
Alternaria brassicicola is a filamentous fungus that causes black spot ­disease on most plants in the Brassicaceae, including cultivated Brassica species and weedy Arabidopsis. Since the concept of transformation constructs of linear minimal elements was developed [1], we have optimized protoplast production by reducing melanin accumulation in fung...
Article
Full-text available
Brassinin is an antifungal compound induced in Brassica plants after microbial infection. Molecular evidence is incomplete, however, in supporting the importance of brassinin in plant resistance to pathogens. To test the importance of brassinin in plant defense, we studied the functions of the gene Bdtf1 in the necrotrophic fungus, Alternaria brass...
Article
Alternaria brassicicola is a successful saprophyte and necrotrophic plant pathogen. To identify molecular determinants of pathogenicity we created nonpathogenic mutants of a transcription factor-coding gene, AbPf2. Frequency and timing of germination and appressorium formation on host plants were similar between the nonpathogenic ∆abpf2 mutants and...
Data
Pigment secreted by Δamr1 mutants and its effect on pathogenesis and host plants. A. Mycelia of the Δamr1 mutant at the bottom of the flask (left) is melanin deficient compared to the dark-colored wild-type mycelia (right). Both were cultured in 1% glucose and 0.5% yeast extract broth (GYEB) for three days, in the dark, with continuous agitation at...
Data
Verification of targeted gene disruption by polymerase chain reaction (PCR). PCR amplification was performed with two pairs of target gene specific and HygB resistance gene primers. Relative primer binding locations are marked below the schematic diagram of the disrupted target gene on the left side of the gel images at the top row of page one. The...
Data
List of genes differentially expressed in the Δ amr1 mutants compared to the wild type. (XLS)
Data
Statistically over-represented functional annotation terms among 101 up-regulated genes in the Δamr1 mutant compared to the wild type. The 101 genes were selected based on the statistical analysis included in Cufflink with at least a two-fold-difference. (DOC)
Data
Creation of Δabvf8 deletion mutants and pathogenicity assays. A. Schematic diagram of the wild-type locus of the AbVf8 gene, a replacement construct, and a mutant locus. The mutant locus depicts incorporation of the replacement construct into a wild-type locus by double homologous recombination resulting in gene replacement. B. Replacement of the A...
Data
Comparison of phenotypes between abpks7 mutants and wild-type Alternaria brassicicola. A. Stereoscopic view of an abpks7 mutant colony showing melanin-deficient aerial hyphae and conidia. B. Stereoscopic view of a wild-type colony showing melanized aerial hyphae and conidia. C. Hyaline conidia of an abpks7 mutant. Mutant conidia were mixed with a s...
Data
List of transcription factor genes whose mutants were screened in this study. (XLS)
Data
PCR verification primers (5′ to 3′ direction). (DOC)
Data
Disruption Primers (5′ to 3′ direction). (DOC)
Data
List of primers used for qRT-PCR and transformation constructs. (DOC)
Article
Full-text available
Alternaria brassicicola is a successful saprophyte and necrotrophic plant pathogen. Several A. brassicicola genes have been characterized as affecting pathogenesis of Brassica species. To study regulatory mechanisms of pathogenesis, we mined 421 genes in silico encoding putative transcription factors in a machine-annotated, draft genome sequence of...
Article
Full-text available
Alternaria brassicicola is a successful saprophyte and necrotrophic plant pathogen with a broad host range within the family Brassicaceae. It produces secondary metabolites that marginally affect virulence. Cell wall-degrading enzymes (CDWE) have been considered important for pathogenesis but none of them individually have been identified as signif...

Questions

Question (1)
Question
We ran western blots for low molecular weight proteins from exosomes. While running in gel we observe some retardation and wavy movement of samples which also shows up in the blots while samples from cell lysates run normally in the same gel. Any thought of the possible reasons and measures to troubleshoot? Exosomes were isolate by ultracentrifugation method re-suspended in RIPA buffer, sonicated and boiled before loading on gel 

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Projects (2)
Project
How exosomes are regulating immune system to promote cancer growth