Akarin Boonsombuti

Chalmers University of Technology

I used novozym50013 as cellulase enzyme to digest corncobs from acid pretreatment but the bacterial cannot grow in that obtained medium. Firstly I looked into the enzyme datasheet provided data about it contained potassium sorbate as preservative that may inhibit my culture but after looking through the literature there are someone use to grow culture in the obtained medium like me (not same strain but it is also bacteria).

Should I purchase new enzyme that previously report with bacteria I used because the medium obtained from acid pretreatment is working well so I think the problem is from the enzyme. Is there anyone who used to use this enzyme?