Akanksha Roberts

National Institute of Animal Biotechnology · Department of Diagnostics

Fluorine doped tin oxide (FTO) electrochemical immunosensor has been developed for rapid detection of urokinase type plasminogen activator receptor (uPAR) - a biomarker for cancer. uPAR is a GPI-anchored cell membrane receptor that shows increased expression in many types of human cancers which include breast, prostate, colorectal, and non-small ce...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, also known as 2019-nCov or nCovid-19) outbreak has become a huge public health issue due to its rapid transmission making it a global pandemic. Currently, there are no vaccines or drugs available for nCovid-19, hence early detection is crucial to help and manage the outbreak. Here, we rep...

Graphene, a two-dimensional nanomaterial, has gained immense interest in biosensing applications due to its large surface-to-volume ratio, and excellent electrical properties. Herein, a compact and user-friendly graphene field effect transistor (GraFET) based ultrasensitive biosensor has been developed for detecting Japanese Encephalitis Virus (JEV...

One of the greatest challenges faced during surgical procedures is closing and healing of wounds, which are essential in the field of orthopaedics, trauma, intensive care and general surgery. One of the main causes of death has been linked to chronic wounds, especially in immunosuppressant or diabetic patients. Due to increasing chronic wound fatal...

β-Secretase1 (BACE1) catalyzes the rate-limiting step in the generation of amyloid-β peptides, that is, the principal component involved in the pathology of Alzheimer’s disease (AD). Recent research studies show correlation between blood and cerebrospinal fluid (CSF) levels of BACE1 with the pathophysiology of AD. In this study, we report one-step...

Advanced biosensor technology research is imperative for the management of infectious disease outbreaks such as Japanese Encephalitis (JE), a zoonotic disease caused by the flavivirus JE virus (JEV) which is transmitted to humans (dead-end hosts) from the amplification host, pigs, via mosquitoes. To avoid future pandemic scenarios, proactive resear...

Lateral flow assays (LFAs) are one of the most economical, point-of-care (PoC) diagnostic assays that exploit the colorimetric properties of gold nanoparticles (AuNPs). Up to the best of our knowledge, no rapid antigen-based LFA exists for Japanese Encephalitis Virus (JEV) detection. Herein, we have reported a novel portable sandwich-type LFA for o...

The unregulated usage of Cephalexin (CFX) in animal source food products lead to antimicrobial resistance (AMR) in humans. Graphene quantum dots (GQD) are zero-dimensional nanomaterials possessing both unique optical and electrical properties based on their tuneable size that serves as an excellent sensor. The fluorescence quenching and conductive...

The impact of uncontrolled antibiotic use in animals subsequently led to emergence of antibiotic-resistant bacteria among humans due to consumption of animal products and its by-products. Hence, to investigate antibiotic contamination in animal origin food products, we have developed a reduced graphene oxide (rGO) based immunosensor using Fluorine-...

Advanced diagnostic technologies for early-stage detection of cancer are essential for proper management and guided therapeutics to increase chances of survival for patients. Presently, most forms of cancer get diagnosed post metastasis throughout the body resulting in late-stage treatment leading to fatality which may have otherwise been avoided....

Japanese Encephalitis Virus (JEV), a zoonotic infectious disease is a major concern in East-Asian and Western Pacific regions with no available therapy or fool-proof vaccines. Hence it is imperative to develop diagnostic assays for rapid mass screening for JEV biomarkers in human as well as pigs for better management of this recurring epidemic and...

Salmonellosis is a symptomatic infection, a foodborne disease, caused by Salmonella that enters the body through the ingestion of contaminated food. In this study, a novel electrochemical biosensor integrated with gold nanorods (GNRs) was used to explore the interaction between in-house generated antibodies with Salmonella serovars. Under optimal c...

Coronavirus Disease 2019 (COVID-19) pandemic has shown the need for early diagnosis to manage infectious disease outbreaks. Here, we report a label free electrochemical Fluorine-Doped Tin Oxide (FTO) Immunosensor coupled with gold nanorods (GNRs) as an electron carrier for ultrasensitive detection of the Receptor Binding Domain (RBD) of SARS CoV-2...

Compared to other nanomaterial, surface-modified iron oxide nanoparticles (IONPs) have gained attraction for cancer therapy applications due to its low toxicity, and long retention time. An innocuous targeting strategy was developed by generation of Fluorescein isothiocyanate (FITC) labeled peptide (Growth factor domain (GFD) and somatomedin B doma...

Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2, also referred to as 2019-nCov or COVID-19) outbreak has become a huge public health issue due to its rapid transmission, making it a global pandemic. To manage the outbreak, it is the need of the hour to develop biosensing techniques for rapid, sensitive and efficient detection of the vir...

Advancement in lab-on-a-chip (LOC) and microfluidics technologies has led to the development of microscale cell culture platforms leading to physiological organ biomimetic systems built on microfluidic chips known as organ-on-chip (OOC). This integration of both electronic and fluidic components onto a single chip using a combination of bioengineer...

Coronavirus disease 2019 (COVID-19), which started out as an outbreak of pneumonia, has now turned into a pandemic due to its rapid transmission. Besides developing a vaccine, rapid, accurate, and cost-effective diagnosis is essential for monitoring and combating the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its rel...

Fluorine Doped Tin Oxide (FTO) electrode was fabricated with reduced Graphene Oxide (rGO) for sensitive detection of Japanese encephalitis virus (JEV) non-structural 1 (NS1) protein. Beforehand, in-silico 3D structure, stability, and docking of recombinant JEV NS1 antigen (NS1–Ag) and antibody (Ab) was evaluated. The recombinant NS1 Ag of 42 kDa wa...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, also known as 2019-nCov or COVID-19) outbreak has become a huge public health issue due to its rapid transmission making it a global pandemic. Here, we report fabricated fluorine doped tin oxide (FTO) electrodes/gold nanoparticles (AuNPs) complex coupled with in-house developed SARS-CoV-2...

Immobilization techniques involve the confining/anchoring of a molecule (enzyme or cells) in or on an inert support/substrate matrix for stability, increased efficiency and easy recoverability of the molecule. The immobilization of molecules to a substrate is carried out on the basis of five major principle methods, i.e. adsorption, entrapment, mem...

Graphene, a two-dimensional nanomaterial, has gained immense interest in biosensing applications due to its large surface-to-volume ratio, and excellent electrical properties. Herein, a compact and user-friendly graphene field effect transistor (GraFET) based ultrasensitive biosensor has been developed for detecting Japanese Encephalitis Virus (JEV...

Japanese Encephalitis Virus (JEV) is the most common Flavivirus based mosquito borne viral encephalitis in the world, especially in countries of South-East Asia. The conventional methods such as Enzyme-Linked Immunosorbent Assays (ELISA), Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), Plaque Reduction Neutralization Test and virus isolat...

Human immunodeficiency virus (HIV), a type of lentivirus (a subgroup of retrovirus), causes acquired immunodeficiency syndrome (AIDS). This pathophysiologic state destroys the immune system allowing opportunistic infections, cancer and other life-threatening diseases to thrive. Although many analytic tools including enzyme-linked immunoassay (ELISA...

The two dimensional, honeycomb structured, single carbon layered graphene has extensively been used in the field of sensor detection due to its unique physicochemical properties. These properties such as excellent electrical conductivity, high electron mobility, tunable optical properties, room temperature quantum Hall effect, large surface to volu...