Akalpita Arvindekar

Akalpita Arvindekar
Shivaji University, Kolhapur · Department of Bio-Chemistry

M.Sc. Ph.D

About

34
Publications
19,394
Reads
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905
Citations
Citations since 2017
10 Research Items
607 Citations
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
Introduction
We are working on Ayurvedic medicines in treatment of diabetes. Currently two major research projects are ongoing - UGC (14.97 lakhs) on protein glycation inhibition and other from RGSTC (100.90 lakhs) for establishing a resource centre for ayurvedic medicine for diabetes treatment, advanced studies, human trials and technology transfer. Current thrust area is insulin mimetics and protein glycation studies.
Additional affiliations
June 2016 - February 2019
Shivaji University, Kolhapur
Position
  • Head of Faculty
January 2002 - December 2013
Shivaji University, Kolhapur

Publications

Publications (34)
Article
Type 1 diabetes mellitus is an autoimmune disorder leading to loss of beta cells. There is a dire need to inhibit apoptosis and induce regeneration of new beta cells. There are plants in the Indian medicine system having the potential for rejuvenation. In the present study, we have attempted to evaluate the capacity of aqueous extract of Tinospora...
Article
Full-text available
Background Type 1 diabetes mellitus is characterized by loss of beta cell mass and insulin insufficiency. Beta cell apoptosis and destruction by cytotoxic T lymphocytes and pro-inflammatory cytokines like IL-1β, TNF-α and IFN-γ appears to represent a key event in pathogenesis of type 1 diabetes mellitus. Purpose The aim of present study was to eva...
Article
Curd and yogurt is rich in protein, calcium, magnesium and other vitamins and considered as beneficial for diabetics. The present study delves into another aspect of curd and yogurt; viz. its role in protein glycation and thereby initiation of inflammation. Curd contains glyoxal and methylglyoxal and thereby capable of glycating proteins. The prese...
Article
Diabetes and obesity is associated with change in the gut microbiota, however, the reason for such transition is still unknown. The secondary complications in diabetes mainly stem from protein glycation, oxidative stress and in ammatory response. It is intended to study the correlation between gut proteins glycation and microbial dysbiosis and ther...
Article
Introduction Development of therapy options for treatment of type 1 diabetes mellitus is hampered by non-availability of appropriate experimental models that can exactly mimic the in vivo situation. Apoptosis of beta cells by T cells and cytokine action leads to loss of beta cells. We propose a simple and elegant model using cytokine cocktail of TN...
Article
Objective: To isolate and identify the bioactive component from Cymbopogon martinii having GLUT2 transporter inhibitory activity - towards development of a novel strategy for treatment of diabetes mellitus. Method: Isolation of bioactive component was carried out using differential solvent extraction, HPTLC and HPLC, and identification was done...
Article
Full-text available
Hyperglycemia in diabetes causes protein glycation that leads to oxidative stress, release of cytokines, and establishment of secondary complications such as neuropathy, retinopathy, and nephropathy. Several other metabolic disorders, stress, and inflammation generate free radicals and oxidative stress. It is essential to study whether oxidative st...
Article
Aims Prediabetes manifests several years earlier, before it progresses to diabetes. It is essential to track the earliest metabolic changes occurring in the prediabetic state and to understand the precise mechanism of how diabetes is initiated. Main methods Alpha glucosidase was isolated from rat intestine and assayed using maltose as substrate....
Article
Full-text available
Objective: To evaluate the use of protein glycation inhibitors and probiotics to ameliorate secondary complications in diabetes and to improve gut microbiota respectively in high fructose fed Wistar rat. Methods: The study was conducted on male Wistar rats for 7 d. Blood glucose levels in oral glucose tolerance test (OGTT) were measured using gluco...
Article
Full-text available
Rheum emodi is used as a culinary plant across the world and finds an eminent role in the traditional Ayurvedic and Chinese system of medicine. The plant is known to principally contain 1,8-dihydroxyanthraquinones (DHAQs) like rhein, aloe emodin, emodin, chrysophanol and physcion that possess diverse pharmacological and therapeutic actions. The pre...
Article
Full-text available
Objective: The present study deals with identification of bioactive component from the distillate of Cymbopogon citratus plant and to evaluate its aldose reductase inhibitory and antioxidant activity. Methods: The aldose reductase enzyme was semi purified by ammonium sulphate precipitation from chicken eye lens. Differential solvent extraction of C...
Article
Full-text available
Maesa indica belonging to family Myrsinaceae, commonly known as Atki is a large shrub. Ethnobotanical studies of Kolhapur district revealed that stem bark of M. indica is used in the treatment a diabetes. The literature survey revealed that there is no experimental evidence of antidiabetic effect of the plant. Therefore the present study was carrie...
Article
Full-text available
Objective: Oxidative stress and Advanced Glycation End-products have been associated with diabetic complications. Therefore, natural compounds or extracts that possess both antioxidant and anti-glycation activities might have great therapeutic potential for treating diabetic complications. Methods: The main purpose of this study was to evaluate the...
Article
Full-text available
Objective: To demonstrate the efficacy of a protein glycation inhibitor (limonene) and antihyperglycemic plant component (linalool) in combination towards better management of diabetes. Methods: Antihyperglycemic activity was assessed by OGTT and glucose uptake studies on diaphragm tissue. Protein glycation inhibition was studied using BSA as a mod...
Article
Context: Kalanchoe pinnata Lam. (Crassulaceae) is used as a traditional medicine worldwide to treat several ailments, including diabetes. However, the mechanism for the antihyperglycemic action is unknown. Objective: The present study evaluates the antihyperglycemic and insulin secretagogue potential of Kalanchoe pinnata and assessment of the pr...
Article
Inhibition of protein glycation is known to ameliorate secondary complications in diabetes. In the present study antiglycative properties of limonene, a natural product, were evaluated using BSA as a model protein. AMG (aminoguanidine) was used as a positive control. Measurement of total AGEs (Advanced Glycation End-products) and specific AGEs reve...
Article
To study the antiglycating, antidiabetic and antioxidant properties of Aegle marmelos Correa leaf extract and identify the bioactive constituent. The effect of the chloroform extract of Aegle marmelos Correa was studied in streptozotocin-induced diabetic rats through evaluation of biochemical parameters. Antiglycation activity was assessed in vitro...
Article
Cuminum cyminum, a commonly used spice, is known to have anti-diabetic action. The present study aims towards the isolation of bioactive components from C. cyminum and the evaluation of their insulin secretagogue potential with the probable mechanism and β-cell protective action. The anti-diabetic activity was detected in the petroleum ether (pet e...
Article
Protein glycation inhibition is important to prevent secondary complications in diabetes mellitus. p-Cymene, a monoterpene commonly found in Cuminum cyminum was investigated in vivo and in vitro using aminoguanidine as a positive control. Streptozotocin induced diabetic rats were treated with 20 mg cymene kg−1 body weight for 60 days. Cymene treatm...
Article
Foeniculum vulgare fruits are routinely consumed for their carminative and mouth freshening effect. The plant was evaluated for aldose reductase inhibition and anti diabetic action. Bioguided fractionation using silica gel column chromatography, HPLC, and GC-MS analysis revealed trans-anethole as the bioactive constituent possessing potent aldose r...
Article
Full-text available
Inhibition of intestinal alpha glucosidase plays a major role in preventing rise in postprandial glucose level in diabetics. Cymbopogon martinii (CM) (family Poaceae) is used in traditional Indian medicine in treatment of diabetes mellitus. The alpha glucosidase inhibitory action of the plant is studied. The active component was separated using hot...
Article
Full-text available
Santalum album Linn (Santalaceae), commonly known as Sandalwood is used traditionally for its antihyperlipidemic and diuretic activity. This study investigated the antihyperglycemic and antihyperlipidemic effect of long-term oral administration of the Santalum album pet ether fraction in streptozotocin-induced diabetic rats. Diabetes was induced by...
Article
Optimization of ethanol production through addition of substratum and protein-lipid additives was studied. Oilseed meal extract was used as protein lipid supplement, while rice husk was used as substratum. The effect of oil seed meal extract and rice husk was observed at varying concentration of medium sugar from 8% to 20%. Of the three oil seed me...
Article
Spices are extensively used to enhance the taste and flavor of foods and are known to possess several medicinal properties. Myristica fragrans, Parmelia perlata, Illicium verum, Trachyspermum copticum and Myristica malabarica, the commonly used spices in India were assessed for antidiabetic activity in streptozotocin induced diabetic rats. In the i...
Article
Glycogen in Saccharomyces cerevisiae is present in two pools as cell wall bound and intracellular glycogen. The content of the cell wall bound glycogen was found to be almost three times higher than the intracellular glycogen. The content varied with the sugar concentration in the medium and an optimum value of 22.11 mg glycogen/g yeast was observe...
Article
Full-text available
Inhibitors of alpha glucosidase have potential use in the treatment of diabetes mellitus. The stem extract of Tinospora cordifolia was evaluated for inhibition of the enzyme. The extract was also found to inhibit the salivary and pancreatic amylase and therefore can effectively reduce an increase in postprandial glucose level. The crude ethyl aceta...
Article
J. Inst. Brew. 114(3), 205–208, 2008 Glycogen is an important storage reserve in yeast. In Saccharo-myces cerevisiae glycogen is present in two pools, an intracellu-lar soluble pool and a cell wall bound, insoluble extra-cellular pool. The present method uses a 20% KOH treatment to separate the two pools, which are then estimated using amyloglucosi...
Article
Novel additives that act as substratum for attachment of the yeast cells, increased ethanol production in Saccharomyces cerevisiae. The addition of 2 g rice husk, straw, wood shavings, plastic pieces or silica gel to 100 ml medium enhanced ethanol production by 30-40 (v/v). Six distillery strains showed an average enhancement of 34 from 4.1 (v/v) i...
Article
Glycogen in Saccharomyces cerevisiae is present in two pools, one soluble and intracellular, the other present in the cell wall and rendered water-insoluble owing to its covalent linkage to cell wall beta-glucan. The insoluble glycogen fraction was solubilized using beta-1,3-glucanase. The alpha beta-glucan complex obtained showed intense red stain...

Questions

Questions (6)
Question
We want to study some antioxidants and assess their relative effectiveness.  However we wish to induce oxidative stress preferably using dietary additives.
Question
We have been working on gluconeogenesis in the liver. We were finding varying results for pyruvate uptake studies. Then we realized that some of the lobes show pyruvate uptake while other do not, hence the variation. Why should this happen?
Question
Ascorbate is an antioxidant and hence should be able to inhibit protein glycation but at the same time ascorbate itself can glycate proteins and further some have reported to be a pro-oxidant
Question
Diet is known to alter the gut microbiota. Is it likely that processed foods and high glycemic index foods may bring about such a change which in turn may lead to inflammatory and other changes progressing to diabetes?
Question
There is plenty of literature available on insulin resistance, however, it only adds to the confusion. I would like to know what the most appropriate method to quantify insulin resistance is.
Question
Are there any findings or reports on changes that occur in the body owing to establishment of Type II diabetes that cannot revert back once they occur?

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Projects

Projects (2)
Project
To prevent progression from pre diabetes to diabetes
Project
To assess nutritional status of wild edible plants. To evaluate antioxidant potential.