Ajit Kumar Shasany

Ajit Kumar Shasany
Central Institute of Medicinal and Aromatic Plants | CIMAP · Department of Plant Biotechnology (CIMAP)

PhD Molecular Biiology and Biotechnology

About

275
Publications
74,691
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4,316
Citations
Citations since 2016
107 Research Items
2431 Citations
20162017201820192020202120220100200300400
20162017201820192020202120220100200300400
20162017201820192020202120220100200300400
20162017201820192020202120220100200300400

Publications

Publications (275)
Article
Full-text available
Main conclusion Several cis-elements including Myb-binding motifs together confer glandular trichome specificity as revealed from heterologous expression and analysis of menthol biosynthesis pathway gene promoters. Abstract Glandular Trichomes (GTs) are result of division of epidermal cells that produce diverse metabolites. Species of mint family...
Preprint
A two-step greener catalytic process has been developed for semi-synthesis of (-)-menthol from citronellal-rich essential oils such as Cymbopogon winterianus and Corymbia citriodora by using novel bi-acidic composites. These novel composites (Al-B-NaYZE or Sn-B-NaYZE) were prepared by impregnation of Sn-B and Al-B over NaYZE framework to increase t...
Article
Full-text available
Management of pod borer, Helicoverpa armigera in pigeonpea (Cajanus cajan L.), an important legume crop, has been a pertinent endeavor globally. As with other crops, wild relatives of pigeonpea are bestowed with various resistance traits that include the ability to deter the H. armigera. Understanding the molecular basis of pod borer resistance cou...
Article
Terpene synthases (TPSs) have diverse biological functions in plants. Though the roles of TPSs in herbivore defense are well established in many plant species, their role in bacterial defense has been scarce and is emerging. Through functional genomics, here we report the in planta role of potato (Solanum tuberosum) terpene synthase (StTPS18) in ba...
Article
Full-text available
A high yielding variety of lemongrass (Cymbopogon khasianus) CIMAP Suwarna suitable for water stress/rainfed/marginal land conditions.2010. J Med. Aromat. Plant Sci. 32 (1): 61-63. Lemongrass, family-Poaceae an important aromatic grass commonly known as "Nibughas" is vegetatively/ seeds propagated perennial, multicut crops in the tropics/subtropic...
Article
Full-text available
The medicinal properties of Ashwagandha (Withania somnifera) are attributed to triterpenoid steroidal lactones, withanolides, which are proposed to be derived from phytosterol pathway, through the action of cytochrome P450 (CYP450) enzymes. Here, we report the characterization of three transcriptome-mined CYP450 genes (WsCYP749B1, WsCYP76 and WsCYP...
Chapter
Catharanthus roseus represents a reservoir of over 130 medicinally important alkaloids, many of which are currently in therapeutic use. It is one of the most extensively investigated medicinal plants and has acquired the status of a model non-model plant due to many decades of active research conducted upon it by various groups across the globe. Ne...
Chapter
Catharanthus roseus is a highly valuable plant often used for its medicinal applications. This plant synthesizes potent antitumor drugs known as vinblastine and vincristine which are produced via monoterpene indole alkaloids (MIAs) biosynthesis pathway. MIA biosynthesis is a complicated pathway that involves about 30 coordinated steps and produces...
Article
Full-text available
Emerging infectious diseases (EID) are serious problems caused by fungi in humans and plants and are examined as a warning to world food security. Plant origin antifungal peptides are biologically active peptides. They play a vital role in the primary host defense against microbial invasion. The Fusarium oxysporumspecies complex includes phytopatho...
Article
Full-text available
Emerging infectious diseases (EID) are serious problems caused by fungi in humans and plant species. They are a severe threat to food security worldwide. In our current work, we have developed a support vector machine (SVM)-based model that attempts to design and predict therapeutic plant-derived antifungal peptides (PhytoAFP). The residue composit...
Article
Full-text available
Long non-coding RNAs (lncRNAs) are an emerging class of non-coding RNAs and potent regulatory elements in the living cells. High throughput RNA sequencing analyses have generated a tremendous amount of transcript sequence data. A large proportion of these transcript sequences does not code for proteins and are known as non-coding RNAs. Among them,...
Article
Full-text available
Catharanthus roseus is known for its scarce and expensive anticancer bisindole alkaloids, vincristine and vinblastine, which are derived from the coupling of monomeric terpenoid indole alkaloids, catharanthine and vindoline. Vindoline represents the bottleneck in bisindole semi-synthesis as unlike catharanthine, its biosynthesis is restricted to th...
Chapter
Full-text available
Andrographis paniculata (Burm.f) Nees (Family: Acanthaceae) is one of the most popular and valuable medicinal plants with high trade value. It is out mentioned in various systems of medicine since ancient times in Indian, Malay, Chinese and Thai. Andrographis paniculata is native to Southeast Asian countries i.e., India, China, Taiwan and Sri Lanka...
Article
Full-text available
Background: Class III plant peroxidases play important role in a number of physiological processes in plant such as lignin biosynthesis, suberization, cell wall biosynthesis, reactive oxygen species metabolism and plant defense against pathogens. Peroxidases are also of significance in several industrial applications. In view of this, the producti...
Article
Full-text available
Parthenium hysterophorus L. is a notorious weed, which significantly reduce yield and quality of crops and causes several problems to human health. In, present study an attempt was made to understand the economic value, and survival nature of Parthenium through deep transcriptome analysis. Transcriptome analysis of leaf and root tissue of P. hyster...
Article
Full-text available
Catharanthus roseus produces many terpenoid indole alkaloids (TIAs) but is famous for its scarce and costly anticancer bisindole alkaloids, vinblastine and vincristine that are formed by coupling of monomeric TIAs, catharanthine and vindoline. Biosynthetic potential of the plant for bisindoles is very low due to their high cytotoxicity. The best op...
Article
Full-text available
Ocimum is one of the most revered medicinally useful plants which have various species. Each of the species is distinct in terms of metabolite composition as well as the medicinal property. Some basil types are used more often as an aromatic and flavoring ingredient. It would be informative to know relatedness among the species which though belong...
Article
Full-text available
Ocimum tenuiflorum is a widely used medicinal plant since ancient times and still continues to be irreplaceable due to its properties. The plant has been explored chemically and pharmacologically, however, the molecular studies have been started lately. In an attempt to get a comprehensive overview of the abiotic stress response in O. tenuiflorum,...
Preprint
According to WHO, the current Coronavirus disease situation is 8,506,107 confirmed and 455,231 death cases in approx 216 Countries, areas, or territories. For the treatment of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) drug repurposing seems to be an effective strategy as it could shorten the time and reduce the cost compared to d...
Preprint
The aim of this study was to determine the antibacterial property of extract derived from a part of the Jackfruit called rag, that is generally considered as fruit waste. Morpho-physical characterization of the Jackfruit rag extract (JFRE) was performed using gas-chromatography, where peaks indicative of furfural; pentanoic acid; and hexadecanoic a...
Article
Full-text available
Emerging infectious diseases (EIDs) are a severe problem caused by fungi in human and plant species across the world. They pose a worldwide threat to food security as well as human health. Fungal infections are increasing now day by day worldwide, and the current antimycotic drugs are not effective due to the emergence of resistant strains. Therefo...
Article
Full-text available
Azospirillum brasilense is used worldwide as a plant growth-promoting inoculant for agricultural crops. To understand how the genomes of Indian strains of A. brasilense compare with their South American counterparts, we determined the whole-genome sequences of four strains of A. brasilense isolated from the rhizosphere of grasses from India.
Article
Full-text available
Key message A class III peroxidase from Artemisia annua has been shown to indicate the possibility of cellular localization-based role diversity, which may have implications in artemisinin catabolism as well as lignification. Abstract Artemisia annua derives its importance from the antimalarial artemisinin. The –O–O– linkage in artemisinin makes...
Article
Catharanthus roseus is known for its anticancer bisindole alkaloids, vinblastine and vincristine that are derived from the condensation of monomeric terpenoid indole alkaloids (TIAs), catharanthine and vindoline. The in planta content of TIAs varies with seasons and ontogeny making it difficult for growers to define the optimal parameters for harve...
Article
Full-text available
Ocimum tenuiflorum is a widely used medicinal plant since ancient times and still continues to be irreplaceable due to its properties. The plant has been explored chemically and pharmacologically, however, the molecular studies have been started lately. In an attempt to get a comprehensive overview of the abiotic stress response in O. tenuiflorum,...
Data
Summary of unigene and CDS statistics. (DOCX)
Data
Transcript annotation file. (XLSX)
Data
DGE with annotation Control Vs. Drought. (XLSX)
Data
Statistical summary of RNA sequencing and assembly. (DOCX)
Data
Commonly up-regulated and down-regulated transcripts in all the four abiotic stresses (Cold, Flood, Drought and Salt). (DOCX)
Data
GO distribution for CDS. (DOCX)
Data
DGE with annotation Control Vs. Cold. (XLSX)
Data
List of primers used in the study. (DOCX)
Data
Sequences of the CDS discussed in the manuscript. (DOCX)
Data
Summary of coding sequences (CDS) predicted from unigenes and their annotations. (DOCX)
Data
qRT- PCR for determining the optimal stress conditions for selection of samples for transcriptome sequencing. (PPTX)
Data
GO enrichment analyses of Molecular Function (MF) terms using REVIGO. (A) Significantly enriched transcripts in MF GO terms related to cold treatment. (B) Significantly enriched transcripts in MF GO terms related to flood treatment. (C) Significantly enriched transcripts in MF GO terms related to drought treatment. (D) Significantly enriched transc...
Data
DGE with annotation Control Vs. Flood. (XLSX)
Data
DGE with annotation Control Vs. Salt. (XLSX)
Data
KEGG pathway analysis data. (XLSX)
Data
Annotations of the transcripts used in Heatmap generation. (XLSX)
Data
Distribution number of coding sequences (CDS) and unigene according to their length. (A) Cold, (B) Flood, (C) Drought, (D) Salt, and (E) Control. Blue bar = Unigene, Red bar = CDS. (PPTX)
Data
KEGG pathway distribution of CDSs in all the four abiotic stress samples and control. (PPTX)
Article
The medicinal properties of Ashwagandha (Withania somnifera) are accredited to a group of compounds called as withanolides. 24-methylene cholesterol is the intermediate for sterol biosynthesis and a proposed precursor of withanolides biogenesis. But, conversion of 24-methylene cholesterol to withaferin A and other withanolides has not yet been bioc...
Poster
Ashwagandha (Withania somnifera) is a medicinal plant of high repute, extensively used in most Ayurvedic formulations and a potent candidate of therapeutic potential. Medicinal properties of this plant are attributed to a class of specialized metabolites called withanolides. Withanolides have been extensively studied and reported to have anti-cance...
Data
Representation of O. sanctum OsaTPS partial genes and pseudogenes with subfamily classification. (XLSX)
Data
Multiple sequence alignment was performed and a phylogenetic tree was generated using neighbor joining method with 1,000 replicates for bootstrap values. Complete phylogenetic tree of 14 species terpene synthase genes, including newly sequenced O. sanctum and discovered putative functional 47 OsaTPS was generated. 437 TPS genes were included to gen...
Data
Representation of O. sanctum OsaTPS genes with information of, genome localization, TPS subfamily clusters and gene length. (XLSX)
Data
The combined phylogenetic tree was generated using the Neighbor-joining method with 1,000 bootstrap replicates values. TPS-c, -e and TPS-f subfamily were joined together. OsaTPS1 is used as an outgroup. Terpene synthase genes of O. sanctum was shown in red branch color. (TIF)
Data
Cluster id of all TPS genes from different species included in phylogenetic tree analysis. (XLSX)
Data
All terpene synthase sequences retrieved from different plants species used for phylogenetic analysis. (DOCX)
Data
Phylogenetic rooted tree of all TPS-a subfamily of different species was generated with the Neighbor-joining method, where 1,000 bootstraps replicated were considered and compared with O. sanctum TPS genes which were shown in pink color branches. The bootstrap values ≥80% were designated in red color triangles and values supporting ≥95% were design...
Data
TPS-b and TPS-g subfamily phylogenetic tree were constructed using the Neighbor-joining method with 1,000 bootstrap replicates. O. sanctum TPS genes were shown in pink color branches. TPS-b was subdivided into TPS-b1 and TPS-b2, where TPS-b1 shows only S. lycopersicum species and TPS-b2 show multiple species with 16 TPS genes from O. sanctum. TPS-g...
Data
Representation of O. sanctum OsaTPS genes with annotation, Gene ontology information, sequence alignment distribution and KEGG enzyme mapping. (XLSX)
Article
Full-text available
Holy basil (Ocimum sanctum L.) and sweet basil (Ocimum basilicum L.) are the most commonly grown basil species in India for essential oil production and biosynthesis of potentially volatile and non-volatile phytomolecules with commercial significance. The aroma, flavor and pharmaceutical value of Ocimum species is a significance of its essential oi...
Chapter
The genus Ocimum belongs to the family Lamiaceae, and most popular herb/shrub known for its immense value belongs to their medicinal properties and aroma profile. The presence of vast morphological as well as chemical variability within the genus, its taxonomy, and phylogenetic relationships is still debatable. Adding to this, chemotypes within the...
Article
Full-text available
Papaver somniferum L. (2n=22) belonging to the family Papaveraceae, is an annual herb cultivated extensively in many countries. P. somniferum is the only species of poppy used to produce opium, and it is believed to have evolved through centuries of breeding and cultivation from the wild Mediterranean strain P. setigerum. The dried juice (latex) fr...
Chapter
Artemisia annua (family Asteraceae) is an important medicinal plant. It produces an array of secondary metabolites, but it is mainly known for the antimalarial phytomolecule, artemisinin, which is an endoperoxide sesquiterpene lactone. Artemisinin and artemisinin-based combination therapies (ACTs) are globally used as antimalarials. Presently, the...
Article
Full-text available
Artemisia annua is well-known for producing the antimalarial phytomolecule, artemisinin. The role of peroxidases has been hypothesized in artemisinin metabolism owing to the presence of an –O–O– linkage in this sesquiterpene lactone. Earlier, using a microarray, we identified differentially expressed genes, including peroxidases, in plant growth st...
Article
Full-text available
Artemisia annua is known to be the source of artemisinin worldwide which is an antimalarial compound but is synthesised in very limited amount in the plant. Most research laid emphasis on the methods of enhancing artemisinin but our study has been planned in a way that it may simultaneously address two problems encountered by the plant. Firstly, to...