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Ajay Narayan Sharma

Ajay Narayan Sharma
Nepal Mediciti Hospital · Molecular and Genomic Pathology

M.Sc.

About

27
Publications
6,188
Reads
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136
Citations
Introduction
Biotechnology graduate | Bioinformatics aspirant | interested in Viruses | interested in Vaccines
Additional affiliations
February 2017 - March 2017
University of California, Davis
Position
  • Research Intern
November 2012 - present
Center for Molecular Dynamics Nepal (CMDN)
Center for Molecular Dynamics Nepal (CMDN)
Position
  • Research Associate
July 2009 - December 2009
Wildlife Institute of India
Position
  • Volunteer

Publications

Publications (27)
Article
Full-text available
In an effort to strengthen global capacity to prevent, detect, and control infectious diseases in animals and people, the United States Agency for International Development’s (USAID) Emerging Pandemic Threats (EPT) PREDICT project funded development of regional, national, and local One Health capacities for early disease detection, rapid response,...
Article
Full-text available
In Nepal, rapid urbanization and rural-to-urban migration especially due to internal civil conflict have catalyzed the development of temporary settlements, often along rivers on undeveloped land. This study conducted surveillance for viruses in small mammals and assessed potential risks for virus transmission to people in urban settlements along r...
Article
Full-text available
Bengal tigers (Panthera tigris tigris) serve a pivotal role as an apex predator in forest ecosystems. To increase our knowledge on factors impacting the viability and health of this endangered species, we studied the gut microbiota in 32 individual Bengal tigers from three geographically separated areas (Chitwan National Park (CNP), Bardia National...
Article
Full-text available
Recent molecular systematic studies of arvicoline voles of the genera Neodon, Lasiopodomys, Phaiomys, and Microtus from Central Asia suggest the inclusion of Phaiomys leucurus, Microtus clarkei, and Lasiopodomys fuscus into Neodon and moving Neodon juldaschi into Microtus (Blanfordimys). In addition, three new species of Neodon (N. linzhiensis, N....
Research
Full-text available
The phylogenetic clustering of the identified H9 segment to that of other prevalent AIV isolated from wild and domestic birds of mixed geographical origin suggest a possible role of migratory birds in worldwide viral spread.
Research
Full-text available
The phylogenetic clustering of the identified H9 segment to that of other prevalent AIV isolated from wild and domestic birds of mixed geographical origin suggest a possible role of migratory birds in worldwide viral spread
Article
Full-text available
Tiger (Panthera tigris) populations are in danger across their entire range due to habitat loss, poaching and the demand for tiger parts. The Bengal tiger (Panthera tigris tigris) is an endangered apex predator with a population size estimated to be less than 200 in Nepal. In spite of strict wildlife protection laws, illegal trade of tiger parts is...
Article
Full-text available
Tiger (Panthera tigris) populations are in danger across their entire range due to habitat loss, poaching and the demand for tiger parts. The Bengal tiger (Panthera tigris tigris) is an endangered apex predator with a population size estimated to be less than 200 in Nepal. In spite of strict wildlife protection laws, illegal trade of tiger parts is...
Data
A1.5% agarose gel electrophoresis image of tiger species identification PCR targeting cytochrome-b region of the mitochondrial DNA. (DOCX)
Data
Delta K plot of STRUCTUREHARVESTER result demonstrating the optimal value (K = 3) under LOCPRIOR model. (DOCX)
Data
Values of mean log-likelihood and Delta K for each assumed K (K = 1 to 6) of both supervised (with LOCPRIOR) and unsupervised (without LOCPRIOR) models. (DOCX)
Data
Seized tiger skin sample (F-NP-0011) provided by Central Investigation Bureau(CIB) of Nepal. (DOCX)
Data
A 3% agarose gel electrophoresis image of sex identification PCR of tiger forensic samples targeting Amelogenin gene. Females have asingle band at 214 bp and males havetwo bands at 194 bp and 214 bp. (DOCX)
Data
Forensic samples provided by the Central Investigation Bureau(CIB) of Nepal. (DOCX)
Data
Summary of PCR amplification success, genotyping accuracy and genotyping error rates for 8 microsatellite loci for all processed tiger samples (n = 401) collected in three protected areas (Chitwan National Park, Bardia National Park, and Shuklaphanta Wildlife Reserve) to build baseline tiger genetic database. (DOCX)
Data
Phylogenetic tree (UPGMA) generated from Nei’s genetic distance using 8 nuclear DNA microsatellite loci for 120 reference tiger samples and 14 forensic samples. Samples are colored based on sampling locations. Green represents CNP, blue represents BNP, and red represents SWR samples. Forensic samples are in black. Clusters or clades are labeled acc...
Data
Genotype data on 8 microsatellite loci of the identified tiger forensic samples. (DOCX)
Data
Delta K plot of STRUCTUREHARVESTER result demonstrating the optimal value (K = 4) under the model without LOCPRIOR information. (DOCX)
Data
Proportions of scat sampled and tigers identified from those scats across each study sites from NTGP. (DOCX)
Data
Methodology and results of distance-based phylogenetic analysis. (DOC)
Article
Full-text available
The Bengal tiger (Panthera tigris tigris) and the snow leopard (Panthera uncia) are highly endangered apex predators. These charismatic animals are categorized as flagship and umbrella species, and hence are the focus of many conservation programs. Protecting tiger and snow leopard also safeguards the entire habitat in which they reside, including...
Article
Full-text available
Nepal boarders India and China and all three countries lie within the Central Asian Flyway for migratory birds. Novel influenza A H7N9 caused human fatalities in China in 2013. Subclinical infections of influenza A H7N9 in birds and the potential for virus dispersal by migratory birds prompted this study to assess avian H7N9 viral intrusion into Ne...
Article
Full-text available
Nepal boarders India and China and all three countries lie within the Central Asian Flyway for migratory birds. Novel influenza A H7N9 caused human fatalities in China in 2013. Subclinical infections of influenza A H7N9 in birds and the potential for virus dispersal by migratory birds prompted this study to assess avian H7N9 viral intrusion into Ne...
Article
Full-text available
Oxidant/antioxidant balance has been suggested as an important factor for initiation and progression of cancer. The objective of this study was to determine changes in the levels of malondialdehyde (MDA), nitric oxide (NO), total cholesterol, triglycerides, LDL-cholesterol, HDL-cholesterol, total antioxidant capacity (TAC), glutathione peroxidase (...

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Projects

Projects (2)
Project
Typhoid Environmental Surveillance
Project
PREDICT project, part of USAID’s Emerging Pandemic Threats program (EPT), is developing a global early warning system to detect, track, and predict the emergence of new zoonotic pathogens from wildlife that could pose a threat to human health.