Aiko Sada

Aiko Sada
Kumamoto University | Kumadai · International Research Center for Medical Sciences

Ph.D

About

22
Publications
1,762
Reads
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851
Citations
Introduction
My research focuses on elucidating the cellular dynamics and regulatory mechanisms of tissue-resident stem cells. We identified heterogeneous stem cell populations in the mouse skin epidermis (Nat Cell Biol 2016) and established the genetic tools and molecular markers to analyze these cells in vivo. I am currently studying epithelial stem cells in regeneration and aging by integrating interdisciplinary techniques, such as genetic engineering of mice, imaging, omics analysis, glycobiology and bioengineering.
Additional affiliations
October 2019 - present
Kumamoto University
Position
  • Professor (Associate)
April 2016 - September 2019
University of Tsukuba
Position
  • Professor (Assistant)
April 2011 - March 2016
Cornell University
Position
  • PostDoc Position
Description
  • Dr. Tudorita Tumbar’s laboratory
Education
April 2006 - March 2011
The Graduate University for Advanced Studies
Field of study
  • Germ Cell Biology
April 2002 - March 2006
Shizuoka University
Field of study
  • Biology

Publications

Publications (22)
Article
Full-text available
The interfollicular epidermis regenerates from heterogeneous basal skin cell populations that divide at different rates. It has previously been presumed that infrequently dividing basal cells known as label-retaining cells (LRCs) are stem cells, whereas non-LRCs are short-lived progenitors. Here we employ the H2B-GFP pulse-chase system in adult mou...
Article
Full-text available
Delayed wound healing and reduced barrier function with an increased risk of cancer are characteristics of aged skin and one possible mechanism is misregulation or dysfunction of epidermal stem cells during aging. Recent studies have identified heterogeneous stem cell populations within the mouse interfollicular epidermis that are defined by territ...
Article
Full-text available
Aging in the epidermis is marked by a gradual decline in barrier function, impaired wound healing, hair loss, and an increased risk of cancer. This could be due to age‐related changes in the properties of epidermal stem cells and defective interactions with their microenvironment. Currently, no biochemical tools are available to detect and evaluate...
Article
Full-text available
Adult tissues contain label-retaining cells (LRCs) which are relatively slow-cycling and considered to represent a property of tissue stem cells (SCs). In the ocular surface epithelium, LRCs are present in the limbus and conjunctival fornix; however, the character of these LRCs remains unclear due to lack of appropriate molecular markers. Using thr...
Preprint
Tissue stem cells divide infrequently as a protective mechanism against internal and external stresses associated with aging. Here, we demonstrate that slow- and fast-cycling interfollicular epidermal stem cells in mouse skin undergo distinct aging processes. Two years of lineage tracing reveals that Dlx1+ slow-cycling clones expanded into the fast...
Article
Full-text available
The maladaptive remodeling of vessel walls with neointima formation is a common feature of proliferative vascular diseases. It has been proposed that neointima formation is caused by the dedifferentiation of mature smooth muscle cells (SMCs). Recent evidence suggests that adventitial cells also participate in neointima formation; however, their cel...
Article
Full-text available
Stem cell (SC) proliferation and differentiation organize tissue homeostasis. However, how SCs regulate coordinate tissue scaling in dynamic organs remain unknown. Here, we delineate SC regulations in dynamic skin. We found that interfollicular epidermal SCs (IFESCs) shape basal epidermal proliferating clusters (EPCs) in expanding abdominal epiderm...
Preprint
Adult tissues contain label-retaining cell (LRC)s, which are relatively slow-cycling and considered to represent a unique property of tissue stem cell (SC)s. In the ocular surface epithelium, LRCs are detected in the limbus, a boundary between cornea and conjunctiva, and the fornix of the conjunctiva; however, the character of LRCs and identity of...
Article
Full-text available
Epigenetic mechanisms controlling adult mammalian stem cell (SC) dynamics might be critical for tissue regeneration but are poorly understood. Mouse skin and hair follicle SCs (HFSCs) display reduced histone H3 K4me3, K9me3, and K27me3 methylation levels (hypomethylation) preceding hair growth. Chemical inhibition of relevant histone demethylases i...
Poster
Aging in the skin epidermis is marked by a gradual decline in barrier function, impaired wound healing and an increased risk of cancer. This could be due to an age-related change in the property of epidermal stem cells or defective interactions with its microenvironment and/or other cell types in the skin. There have been conflicting reports for ag...
Article
Ectopic calcification occurs during development of chronic kidney disease and has a negative impact on long-term prognosis. The precise molecular mechanism and prevention strategies, however, are not established. Fibulin-7 (Fbln7) is a matricellular protein structurally similar to elastogenic short fibulins, shown to bind dental mesenchymal cells a...
Article
In many adult tissues, homeostasis relies on self-renewing stem cells that are primed for differentiation. The reconciliation mechanisms of these characteristics remain a fundamental question in stem cell biology. We propose that regulation at the post-transcriptional level is essential for homeostasis in murine spermatogonial stem cells (SSCs). He...
Article
Stem cells can self-renew and differentiate over extended periods of time. Understanding how stem cells acquire their fates is a central question in stem cell biology. Early work in Drosophila germ line and neuroblast showed that fate choice is achieved by strict asymmetric divisions that can generate each time one stem and one differentiated cell....
Article
Stem cells are maintained by both stem cell-extrinsic niche signals and stem cell-intrinsic factors. During murine spermatogenesis, glial cell line-derived neurotrophic factor (GDNF) signal emanated from Sertoli cells and germ cell-intrinsic factor NANOS2 represent key regulators for the maintenance of spermatogonial stem cells. However, it remains...
Article
Full-text available
The regulation of gene expression via a 3' untranslated region (UTR) plays essential roles in the discrimination of the germ cell lineage from somatic cells during embryogenesis. This is fundamental to the continuation of a species. Mouse NANOS3 is an essential protein required for the germ cell maintenance and is specifically expressed in these ce...
Article
Stem cells give rise to differentiated cell types but also preserve their undifferentiated state through cell self-renewal. With the use of transgenic mice, we found that the RNA-binding protein NANOS2 is essential for maintaining spermatogonial stem cells. Lineage-tracing analyses revealed that undifferentiated spermatogonia expressing Nanos2 self...
Article
Spermatogonial stem cells (SSCs) reside in undifferentiated type-A spermatogonia and contribute to continuous spermatogenesis by maintaining the balance between self-renewal and differentiation, thereby meeting the biological demand in the testis. Spermatogonia have to date been characterized principally through their morphology, but we herein repo...
Article
The expression of C/EBPalpha, which may govern transcription of mature hepatocyte marker genes, was suppressed in periportal hepatoblasts in mouse liver development, leading to biliary cell differentiation. This study was undertaken to analyze how inactivation of the Cebpa gene affects biliary cell differentiation and gene expression of the regulat...

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Projects

Projects (2)
Project
Comprehensive glycan analysis of epidermal stem cells during aging
Project
We aim to understand the cellular and molecular basis of stem cell aging in three epithelial tissues, skin, oral and eyes, with implications for future treatments of age-related disorders.