Aidan Charles

Aidan Charles
Weill Cornell Medicine | Cornell · Brain and Mind Research Institute

Questions

Questions (2)
Question
We are doing survival surgery on the spinal cords of Sprague-Dawley rats but our animals are not surviving after surgery. We are using isoflurane for 5 minutes in the beginning to induce and then using an I.P injection of a ketamine/xylazine cocktail at 90mg/kg ketamine and 4mg/kg xylazine. We then move them to a cage with a heat lamp. We never had this issue before but now a majority of our rats are dying after the surgery is complete and we don't what we can adjust to keep them alive. 
Question
I am thinking of doing in utero injections of mice to study the effects of a drug on the embryos. However, we hope to use the female mice again after it gives birth to the injected pups. Is it possible for the female mouse to become pregnant again and if so, will the previous injection be a significant confounder to any possible results?