Aida Martinez-Hernandez

Colegio de Postgraduados | CP · Campus Campeche

El género Opuntia spp. es el más grande de la familia Cactaceae, la planta produce un fruto llamado tuna, que tiene importancia económica en diversas regiones del país.

Objective: To increase cold tolerance in floral stems of Lilium sp. 'Indian Summerset' stored for two and four weeks using pre- and postharvest treatments. Design/Methodology/Approach: In the first phase, ascorbic acid (AA; 4 mM), glycine betaine (GB; 100 mM), and distilled water (T) were applied, and pre-conditioning was carried out at 0 ºC for 24...

Background The Calakmul Biosphere Reserve (CBR) is known for its rich animal and plant biodiversity, yet its microbial communities remain largely unknown. The reserve does not possess permanent bodies of water; nevertheless, seasonal depressions associated with fractures create wetlands, known locally as aguadas. Given the recent construction of th...

La enfermedad del Huanglongbing (HLB) de los cítricos se ha considerado como la más destructivas en el mundo, asociada con cuantiosas pérdidas económicas debido a la reducción en la producción y calidad de la fruta, así como a la pérdida de empleos directos e indirectos

Los cítricos son cultivos de gran importancia económica en países con clima tropical y subtropical, y son una fuente de ingresos para millones de personas a lo largo de toda la cadena productiva.

La garrapata Rhipicephalus microplus es uno de los más importantes ectoparásitos de bovinos en nuestro país

Huanglongbing (HLB) is considered the most destructive citrus disease in the world, caused by Candidatus Liberibacter asiaticus (CLas) and spread by the vector Diaphorina citri. HLB has generated economic losses in the citrus industry worldwide. All commercial citrus varieties are susceptible to this disease. The intracellular nature of CLas, a str...

Huanglongbing (HLB) is considered the most destructive citrus disease in the world, caused by Candidatus Liberibacter asiaticus (CLas)and spread by the vector Diaphorina citri. HLB has generated economic losses in the citrus industry worldwide. All commercial citrus varieties are susceptible to this disease. The intracellular nature of CLas, a stri...

Background The mite Tetranychus merganser is considered to be an emerging pest of various crops in tropical countries. It is one of the most detrimental pests in the papaya orchards of some regions of México. The current field control of Tetranychus spp. involves the extensive use of chemicals that have some degree of toxicity to humans or the envi...

Objective. To describe the relevance and some tools of genome mining to explore genetic and molecular determinants encoded in bacterial genomes to address agronomic problems. Design/Methodology/Approach. Literature review of the importance of bacteria as a reservoir of biosynthetic gene clusters (BGC), involved in the production of metabolites with...

La limitante principal por el uso de insecticidas químicos para el control de plagas insectiles sigue siendo la resistencia generada por su uso continuo y desmesurado de los agroquímicos.

Conocimiento previo/especie: Agave tequilana Weber var. azul es un importante cultivo en México, utilizado para la producción de tequila. Muchas especies de Agave son tolerantes a condiciones áridas. Sin embargo, las bases moleculares de los mecanismos seleccionados en los agaves para confrontar el estrés abiótico, no han sido descritas. Hipótesis...

Agaves resist extreme heat and drought. In A. tequilana var. azul, the central spike of the rosette -containing the shoot apical meristem and folded leaves in early stages of development- is remarkably heat tolerant. We found that the most abundant protein in this organ is a 27 kDa protein. This protein was named mayahuelin to honor Mayáhuel, the a...

SUMMARY Furostanol glycoside 26-O-β-glucosidase (F26G) participates in the last step of steroidal saponin biosynthesis, cleaving the glucose bound to the C26 of the hydroxylin furostanol glucoside to allow the formation of spirostane. Despite the existence of numerous studies showing the structural diversity and biological activity of plant saponin...

La furostanol glicósido 26-O-β-glucosidasa (F26G) participa en el último paso de la biosíntesis de saponinas esteroidales, lo que escinde la glucosa unida al hidroxilo del C26 del furostanol glucósido para permitir la formación del espirostano. A pesar de la existencia de numerosos estudios que muestran la diversidad estructural y actividad biológi...

La furostanol glicósido 26-O-β-glucosidasa (F26G) participa en el último paso de la biosíntesis de saponinas esteroidales, lo que escinde la glucosa unida al hidroxilo del C26 del furostanol glucósido para permitir la formación del espirostano. A pesar de la existencia de numerosos estudios que muestran la diversidad estructural y actividad biológi...

El principal problema con el uso insecticidas químicos para el control de plagas insectiles sigue siendo la resistencia generada por el uso continuo y desmesurado de los agroquímicos

Mexico has a wealth of Capsicum species, which has led to the development of a large number of chili pepper landraces. A great wealth of Capsicum germplasm can be found in southern Mexico in the Yucatan Peninsula, an important area of diversification of Capsicum annuum. Specifically, in the western Yucatan Peninsula, three of the five domesticated...

The chemical and biological characterization of peptide and protein components of the paralyzing venom from three Pompilidae solitary spider wasps (Pepsis mexicana, Pepsis terminata, and Anoplius nigritus) is described for the first time. The molecular masses of the most abundant peptides were determined. The N-terminal sequences of two cysteine-ri...

The chemical and biological characterization of peptide and protein components of the paralyzing venom from three Pompilidae solitary spider wasps (Pepsis mexicana, Pepsis terminata, and Anoplius nigritus) is described for the first time. The molecular masses of the most abundant peptides were determined. The N-terminal sequences of two cysteine-ri...

Lead (Pb) is one of the most serious environmental pollutants. The aquatic fern Salvinia minima Baker is capable to hyper-accumulate Pb in their tissues. However, the molecular mechanisms involved in its Pb accumulation and tolerance capacity are not fully understood. In order to investigate the molecular mechanisms that are activated by S. minima...

RESUMEN Los agaves son plantas que pertenecen a un género constituido por numerosas especies, adaptadas para crecer en muy diversos hábitats, algunos con condiciones ambientales extremas. Distintas especies de agave crecen sobre distintos tipos de suelos, algunas en suelos con muy bajo contenido de nutrientes minerales y otras en suelos salinos o e...

Fructans are the main storage polysaccharides found in Agave species. The synthesis of these complex carbohydrates relies on the activities of specific fructosyltransferase enzymes closely related to the hydrolytic invertases. Analysis of Agave tequilana transcriptome data led to the identification of ESTs encoding putative fructosyltransferases an...

Agave tequilana var. azul plant for tissue colection. A) Plant structure, B) Head or “piña", C) Leaves S-Stem, BL- Basal leaf section and ML-middle leaf section. (PDF)

Semiquantitative RT-PCR expression profile of fructosyltransferase genes Atq1-SST-1, Atq1-SST-2, Atq6G-FFT-1 and Atq6G-FFT-2. S-stem, BL-base of leaf, ML-middle leaf. (PDF)

Structural comparisons of recombinant proteins from Agave tequilana with respect to crystallized 1-FEH from Cichorium intybus with and without a histidine tag. A) Atq1-SST2, B) Atq6G-FFT1 and C) AtqCwinv-1. (PDF)

List of functionally characterized proteins included in Figure 2 . (PDF)

Primers used for analysis of expression (qRT-PCR) and for construction of P. pastoris heterologous expression vectors. (PDF)

Accession numbers for FT and invertase sequences used in the comparative analysis. (PDF)

Alignment of two different EST sequences encoding Atq6G-FFT-1 with and without the 9-bp exon of the β-fructosidase motif. The red box indicates the 9 bp exon position. (PDF)

A total of 278 bacterial isolates from the rhizosphere of tropical crops were evaluated for its potential to inhibit in vitro the growth of Colletotrichum gloeosporioides. From these, 31 isolates showed antagonistic activity. Dual confrontation qualitative tests for the 31 isolates resulted in the selection of eight isolates antagonist for C. gloeo...

Different species of Agave are grown commercially in Mexico for the production of alcoholic beverages and fibers. These plants are well adapted to dry, arid conditions and can be cultivated on land which is unsuitable for staple crops such as corn or beans. A substantial amount of waste material in the form of discarded leaves or stem tissue is pro...

Bulbil formation in Agave tequilana was analysed with the objective of understanding this phenomenon at the molecular and cellular levels. Bulbils formed 14–45 d after induction and were associated with rearrangements in tissue structure and accelerated cell multiplication. Changes at the cellular level during bulbil development were documented by...

Mexico is the center of origin and diversification of agave, a highly valued crop in the agroindustry, as well as in Mexican culture. Agaves present unique biological characteristics, such as their high tolerance to abiotic stress that allows them to grow under extreme environmental conditions, and their secondary metabolism that leads to the produ...

The relationship between accumulation of Pb(2+) and the activation of chelation and metal sequestration mechanisms mediated by phytochelatins (PC) was analyzed in the Pb(2+) hyperaccumulator aquatic fern Salvinia minima, after exposure to 40microM Pb(NO(3))(2). The tissue accumulation pattern of lead and the phytochelatin biosynthesis responses wer...

We aimed at determining the antagonistic behavior of bacteria derived from marine biofilms against terrestrial phytopathogenic fungi. Some bacteria closely related to Bacillus mojavensis (three isolates) and Bacillus firmus (one isolate) displayed antagonistic activity against Colletotrichum gloeosporioides ATCC 42374, selected as first screen orga...

Light-regulation of photosynthesis-associated nuclear genes is mediated by multipartite cis-regulatory units located in their promoter regions. The combination, spacing, and relative orientation of transcription factor binding sites in these units influences the assembly of specific multiprotein complexes that control gene expression. In this work,...

Los Agaves, plantas que forman parte del patrimonio cultural e histórico de México, son hoy en día importante fuente de derrama económica para diversas regiones del país. Sin embargo, por sus características biológicas: su gran adaptabilidad y capacidad de subsistir aún en ambientes medioambientales extremos, y por sus características metabólicas,...

For the first time, the production of transgenic plants of the forage grass blue grama, Bouteloua gracilis [H.B.K.] Lag. ex Steud., is reported. Transgenic plants containing a gus Colon, two colons nptll fusion driven by a double CaMV35S promoter were obtained by microprojectile bombardment of the highly chlorophyllous embryogenic cell line 'TIANSJ...

Light-inducible promoters are able to respond to a wide spectrum of light through multiple photoreceptor systems. Several cis-acting elements have been identified as components of light-responsive promoter elements; however, none of these regulatory elements by itself appears to be sufficient to confer light responsiveness; rather, the combination...

Recent evidence suggests that a melatonin (MEL) mechanism of action may be through modulation of Ca2+-activated calmodulin (CaM). MEL binds to CaM with a high affinity, and has been shown to act as a CaM antagonist. Among the CaM-dependent enzymes, Ca2+/calmodulin-dependent protein kinase II (CaM-kinase II) is a particularly abundant enzyme in the...