Ahmet Okumuş

• Professor
• Senior Researcher at Independent Researcher

Tomato is one of the most widely cultivated vegetables in our country and around the world. There are various diseases that restrict its production. One of the most important disease agents is Tomato Spotted Wilt Virus (TSWV). TSWV causes wilting and deformation on tomatoes, leading to a decrease in product quality. This viral disease causes signif...

Tomato is one of the most produced vegetables in our country and in the world. There are different diseases that restrict this production. One of the most important of these diseases is root and crown rot diseases caused by Fusarium on tomatos. This disease causes stunting in tomatos seedlings, root rot and necrosis in stem conduction bundles. This...

Tomato is one of the most produced vegetables in our country and in the world. There are different diseases that restrict this production. One of the most important of these diseases is root and crown rot diseases caused by Fusarium on tomatos. This disease causes stunting in tomatos seedlings, root rot and necrosis in stem conduction bundles. This...

Tomato is one of the most widely cultivated vegetables in our country and around the world. There are various diseases that restrict its production. One of the most important disease agents is Tomato Spotted Wilt Virus (TSWV). TSWV causes wilting and deformation on tomatoes, leading to a decrease in product quality. This viral disease causes signif...

Variety identification in plants; It is a clear expression of the differences between plants within the same genus. To identify varieties in plants, phenological morphological and physiological characteristics are used. By taking advantage of these features, different types of feature documents have been prepared by UPOV for each plant species, and...

Variety development in plant breeding is an application that takes many years and requires effort and expense. Variety development includes a technology application that is shaped according to producer and consumer demands, which includes the collection of desired genes from gene pools for agricultural purposes. At the end of this process, the vari...

CRISPR-Cas9-based gene editing is of great interest in the field of agriculture and plant breeding. CRISPR-Cas9 and related gene editing techniques enable precise modifications to plant genomes. This sensitivity allows the insertion or deletion of specific genes into the targeted region, which can achieve desired traits without introducing foreign...

Despite many studies on chestnut cancer and gall wasp, which are the biggest diseases and pests of chestnut production areas in the world and in our country, no results could be obtained. Chestnut cancer is caused by a fungus called Cryphonectria parasitica, while gall damage is caused by a gall wasp called Dryocosmus kuriphilus Yasumatsu. While th...

Cotton plant, one of the most important textile products in the world, is produced for both fiber and oil. Although countries such as China, India, Brazil and the USA realize 75% of cotton plant production, Turkey ranks seventh in production. World cotton production has increased by 5% in recent years with the technological developments. Cotton pro...

Silages are one of the main forage inputs for feeding dairy cattle. Research conducted around the world aims to improve silage quality and minimize nutritional losses during ensiling. Silage inoculants are used to minimize nutritional losses during ensiling by creating a favorable fermentation environment for ensuring the long term storage of the e...

Ruminants are efficient at digesting and converting nutrients from non-edible sources (forages and by-products) through microbial digestion to milk products for human nutrition. Rumination is the rhythmic movement of the digestive system for being very important for optimum feed utilization, milk yield and animal health. Rumination and regurgitatio...

Two different honey bee subspecies’ genotypes obtained from Ordu, Apiculture Research Institute were analyzed by SNP markers using Real-Time PCR-HRM. Genomic DNA samples analysed with 10 SNP primers those were used for identification of chalkbrood disease resistance genes and two SNP primers those were obtained from honey bee genom sequencing. Resu...

High germination rate of seeds is a sought-after feature in the seedling industry. In seed technology, although the seeds with light and empty content are removed while processing the seeds, there is a change in the germination rate due to the maturation of some seeds at different times. In the studies, the drying process applied to the seeds after...

Plant breeding covers a period of time selection and crossing among plant genotypes for higher yield and resistant to diseases. Breeder seed named “elit” is a recent product of breeding process. The faster production of elit seed is so important for marketing of seeds. Synthetic seed technology enables an opportunity for developing elit seeds, easi...

Genetic diversity is getting have been increasingly narrowed by the dense bred cultivar usage for production, on the other hand, genetic variation created by the landraces is known as the raw material of plant breeding. A collection of twentyone-landrace and three-commercial tomato (Lycopersicon esculentum) genotypes were screened for allele profil...

The study was made to establish the genetic variation on chicken genotypes taken place at the villages of Samsun territory, Turkey. In 19 villages, 16 different genotypes (1055) were scored by phenotype observations making comparison by Intervet and Hoecst chicken breed map. Although a regional breed named Gerze (Hacikadin) was seen in these areas,...

Sıvı Solucan Gübresi ve Mikrobiyal Gübre Üretim Makinesi

Hybrid seed was developed by Plant Breeder Ahmet Okumus using two pure tomato lines after crossing. It took 10 years for development and molecular and convensional methods were used.

In order to minimize the problems that may occur during germination and emergence due to adverse environmental factors or directly seed quality and structure, to ensure uniform seedling emergence and strong seedling development in a short time, and to increase resistance to stress conditions (Khan, 1992; Parera and Cantliffe, 1994). Various applica...

The rapid increase of organic wastes in animal farms has made it necessary to make alternative applications for their disposal. Farm wastes, which pose a risk in terms of environmental safety, are consumed by worms after certain processes and transformed into a richer and nutritious form for the plants. Solid worm production facilities, which were...

Soil is a feeding and holding place for growing plants. Plants need many vitamins, enzymes and hormones to feed. The medium that provides this is soil, not only providing the minerals and materials necessary for the plants, but also providing environment for microorganisms with a different living environment. As long as the ecosystem in the soil is...

In addition to their popularity, hydrophonic systems have gained importance in recent years as a result of the need in the food sector due to the decrease in settlements depending on the population. In addition to the increase in production amount alone, the use of less space, hygiene, the use of less pesticides and fertilizers make the hydrophonic...

In addition to their popularity, hydrophonic systems have gained importance in recent years as a result of the need in the food sector due to the decrease in settlements depending on the population. In addition to the increase in production amount alone, the use of less space, hygiene, the use of less pesticides and fertilizers make the hydrophonic...

Soil and Plant Supporters : Bacteria

GENETIC DIVERSITY IN PLANTS. genetic resources and centers, determination of genetic diversity, usage of molecular techniques for genetic diversity, importance of genetic diversity for plant breeding, the conservation of genetic resources and centers, and future of genetic diversity

Organik atıkların tarıma tekrar kazandırılması hakkında hazırlanan projedir.

Son zamanlarda artan tarımsal üretim içerisinde yer alan hayvancılık sektörü beraberinde atık problemini de ortaya çıkarmaktadır. Bu atıkların doğrudan kullanımında ya hastalıkların artışı ya da bitkisel üretim alanında aşırı otlanmaya sebep vermekte, bitkisel ve hayvansal hastalıkların yayılmasına da zemin hazırlamaktadır. Bunların solucanlar tara...

Viruses are one of the most effective harmful organism to be cause of the valuable crops. Tomato spotted wilt virus (TSWV) causes serious diseases of many economically crops such as tomato and lettuce.The TSWV pathogen of tomato was first described in Turkey in 1960. The symptoms of TSWV in tomato vary depending on the stage of growth that the toma...

Tomato is the most grown vegetable in Turkey. On the other hand, the average yield per decare is below the world standards. In our country, tomato production in open field is 3.6 tons per decare, which is lower than the world average (5.06 tons). One of the ways to achieve high yield in tomato production is to use hybrid tomato seeds. Although it p...

Biotechnology is the commercial application of biological phenomena for the benefit of humanity. operates in the fields of genetics, microbiology, cell culture, molecular biology, biochemistry, as well as enzyme engineering, bioprocess engineering, information technology and biorobotics.

Plant reproduction using synthetic seeds has created a new perspective in the agricultural field. In the 1950s, with the observation of embryo and embryo-like structures from callus tissues and the similarity of these structures to the stages of the zygotic embryo, the idea of synthetic seed was revealed. With this technology, seed analogues are cr...

Abstract: Plant biodiversity, enriching ecological systems and environmental health, constitutes subjects of agriculture, industry, medicine and biotechnology; as well as being the future assurance of human life. S. yosgadensis is one of the 55 endemic Salvia species found in Turkey, is a perennial plant which can reach a lenght of 14-30 cm. S. yos...

In this study, we aimed to determine of allelic polymorphisms and genetic relationships among 7 populations generated from congenic village naked neck chicken in Samsun, Ordu and Amasya with Gerze chicken population used as a reference, by 28 microsatellite loci. The number of alleles obtained from all loci was 2 to15. Minimum and maximum number of...

In this study, we aimed to determine of allelic polymorphisms and genetic relationships among 7 populations generated from congenic village naked neck chicken in Samsun, Ordu and Amasya with Gerze chicken population used as a reference, by 28 microsatellite loci. The number of alleles obtained from all loci was 2 to15. Minimum and maximum number of...

Local chicken populations in the Central Black Sea Region of Turkey have been intensively affected by governmental poultry culling due to avian influenza outbreak risks. The aim of this study was to investigate the genetic diversity of indigenous chicken populations raised in the Central Black Sea Region in order to assess genetic structures of the...

In this study we aimed to examine the effects of both Nei’s genetic distance and Bray Curtis distance to calculate chicken diversity data vs geographical distance with Mantel test. Many researchers used Nei’s genetic distance which measure that genetic differences arise due to mutations and genetic drift but some researchers used Bray Curtis distan...

In this study we aimed to examine the effects of both Nei’s genetic distance and Bray Curtis distance to calculate chicken diversity data vs geographical distance with Mantel test. Many researchers used Nei’s genetic distance which measure that genetic differences arise due to mutations and genetic drift but some researchers used Bray Curtis distan...

Here we report phenotypic and genotypic differences among 14 Turkish chestnut genotypes. The genotypes were analyzed both genetically and for 30 morphological criteria comprising 12 qualitative and 18 quantitative characteristics. The phylogenetic relationships were determined between different chestnut genotypes selected from Sinop, Samsun, Artvin...

ABSTRACT: In the study, it was stated that the Mx gene is a gene region that produces antiviral protein in many organisms, and it has been reported that there are positive correlations between Mx gene variations and resistance to diseases. Forty pure Gerze chickens were used in the study. Mx gene region was amplified by PCR method using gene-specif...

A collection of kale (Brassica oleracea var. acephala L.) genetic resources including 20 types selected from 127 populations in terms of yield and leaf quality characteristics from the Black Sea Region of Turkey was evaluated at the DNA level using randomly amplified polymorphic DNA (RAPD) markers compared to some morphological characters. The 7 pr...

The genetic polymorphism of naked village chickens in the Blacksea region of Turkey was analysed using 20 SSR markers with wild type genotypes. Genotypes selected from 445 village chickens were compared with each other to find the genetic variability. The number of polymorphic alleles observed in the naked neck village chickens ranged from 4 to 8 ;...

Definition and genetic similarity between twentyone local populations and three commercial genotypes collected from different regions in Turkey were investigated with the aim of identifying genetic variability in tomato (Solanum lycopersicum) populations at the genetic level using forty SSR loci. Number of alleles per locus ranged from 13 (LEat018)...

Turkish maize accessions collected from north of Turkey were investigated for genetic variation in three maize varieties (flint, pop, dent) including 79 accessions using 8 agronomic and morphologic traits by ANOVA and multivariate discriminant function analysis. Varieties and accessions were significantly varied for variance components which were m...

The honeybee production is to come into question as one of the most important economical sources in our country as being in the world. Up to date, twentyseven honeybee breeds are used for this purposes in the world. In Turkey, it is known that honeybee breeds are spreaded according to regions; Apis mellifera anatolica at the sea side of Trakya, Eag...

Corn is one of the most produced plants in the world as human and animal food after wheat. With the thought of being advantageous in terms of resistance to diseases, the production of the maize plant with gene transfer is carried out in certain countries, and monitoring within the scope of biosecurity is obligatory in line with the decisions taken....

Glucose-6-phosphate dehydrogenase (G6PD) was purified and characterized from the Turkish native chicken, Gerze, erythrocytes for the first time, and some characteristics were investigated. Purification procedure consisted of ammonium sulphate fractionation and affinity chromatography on 29, 59-ADP Sepharose-4B. The enzyme was purified 1063.22-fold...

In this study, 14 sweet cherry cultivars grown in Amasya were identified by RAPD (Random Amplified Polymorphic DNA) markers and the genetic relationships among the cultivars were determined. Twenty-one RAPD primers were screened and eleven primers produced useful amplification for PCR. A total of 68 bands (43 polymorphic) were obtained, with varied...

A REPORT FOR DAAD ON THE PROJECT PROPOSAL This report was prepeared for the analysis of AFLP on maize and phaseolus lines using 40 primer combinations. The primer combinations were selected due to the references of published papers. So, the analysis was done successfully with the usage of kind facilities provided by Prof. Dr. Frank ORDON. Both stu...

Genetic relationships among 21 barley accessions (17 of bulbous barley H. bulbosum L. and 4 of cultivated barley (H. vulgare L.) collected from different part of Turkey were investigated using Random Amplified Polymorphic DNA (RAPD). Eleven informative primers amplified 111 markers of which 98 (89.8%) were polymorphic. A dendogram was constructed u...

In this study, genetically modified organisms and methods for their detection are explained. It has been explained that in recent years, various studies have been carried out on the effects of GMO products on our lives, especially on animals fed with these plants, and whether they are transferred to animal products. It has been reported that there...

Mean of GMO and effects on health is depated

In this study, genetic differences in rice varieties grown in the Black Sea Region were revealed and it was determined in which subspecies these varieties are located. The RAPD technique, which is more suitable than other molecular techniques, has been used in the monitoring of genetic material, depending on the possibilities.

Bird flu has had a significant negative impact on the poultry activities carried out in both commercial companies and small family businesses in our country as well as all over the world. As is known, our country is on the migration routes of migratory birds. The H5N1 type bird flu virus is also mostly carried to distant geographies by migratory bi...

Poultry activities in small family farms, called "village poultry", are more open to the risk of contamination than commercial production businesses. For this reason, the Ministry of Agriculture has resorted to the culling of domestic poultry, to be carried out more intensively, especially in regions where animals carrying viruses are detected. The...

USAGE OF MOLECULAR TECHNIQUES IN HONEYBEE BREEDING IS PRESENTED IN THIS ISSUE.

Turkish Karayaka sheep breed that is one of the important native breed at Black Sea Region in Turkey was genotyped for 13 RAPD primers. Genotypes of 100 were collected from five different herds (BA, BF, LA, LD and KP herds) localized in the Samsun territory. The genetic similarity was obtained on the dendrograms individuals and herd. Within herds,...

A comparative characterization of 17 flint maize landraces (Zea mays L.) was carried out using RAPD markers. Fourteen primers giving reliable and consistent polymorphic bands amplified 125 fragments (89%) with an average of 8.90 fragment per primer. Genetic variation in 17 maize landraces was characterized based on dissimilarity matrix by UPGMA den...

Nicotiana tabacum (2n = 48) is a natural amphidiploid and shows a distribution over a geographical area in eastern Anatolia. Random amplified polymorphic DNA (RAPD) technique was used to evaluate both genetic diversity among 21 primitive tobacco accessions comparing flue cure virginia genotype (FCV) and their geographical polymorphism as a source o...

Maize diversity and molecular studies for breeding on it.

Presentation covers the mean of birdflue, its genetics and how to resistance is important.

ABSTRACT: In this presentation, starting from the origin of the corn plant and its arrival in our country, the first breeding Along with the studies, modern breeding studies applied in this plant today have been compiled. The use of molecular marker data in the detection of parental lines in maize breeding in recent years examples are given. In add...

In this study, Randomly Multiplexed Polymorphic DNA (RAPD) method was first used in the 1990s. It has been widely used in genetic difference and linkage mapping in recent years. It has been stated that it is one of the applications of molecular genetics. The essence of the method is that single chain and random attachment of short oligonucleotides...

In the presentation, the basis of the SSR (Simple sequence repeats) method was explained, and it was stated that DNA contains repetitive regions between or above genes, and these regions show variation in sequence and length between genotypes. It has been reported that variation can be determined at the molecular level based on the fact that repeti...

Twenty populations of kale (Brassica oleracea var. acephala L.) selected from 127 populations in terms of yield and leaf quality characteristics as superior types using weight-based ranking method from the Black Sea Region of Turkey were evaluated at the DNA level using randomly amplified polymorphic DNA (RAPD) markers compared to some morphologica...

how to make gene transfer in plants and its technology

usage of molecular techniques on the utilisation of genetic resources

Starting from Mendelian genetics, the work, which contains the basic information required for plant and animal breeding, gives information about molecular techniques, their use in breeding, gene sequencing, gene transfer and their use in breeding. The book consists of 14 chapters. It is the book of Genetics, which is the unique work of Ahmet Okumu...

In the analysis of genetic differences in two-row and six-row Barley genotypes grown in the Black Sea region by SSR (Simple Sequence Repeats-microsatellites) and RAPD (random amplified polymorphic DNA-randomly amplified polymorphic DNA) methods, 11 SSR markers and 11 RAPD primers were used in total 8 evaluated in genotype. Differences between genot...

Twelve primers were used to characterize the genetic similarity among ten chickens which named RIR I, RIR II, Barred I, Barred II, Colombian Rock, Line-54, Blue Line, Maroon Line, Black Line and Brown Line provided by Ankara Poultry Research Institute-Turkey. The objective of �this study were to determine PCR-based genetic similarity between lines....

Seed storage proteins of 7 landrace accessions of four vetch (Vicia) species collected in Turkey were analyzed by SDS-PAGE. The protein bands of the genotypes were scored for their absence and presence and used for estimating genetic distances which were utilized for a similarity matrix analysis. The genotypes of Vicia spp. showed genetic similarit...

GMO food and their effects

Seed storage protein polymorphisms of five commercial cultivars and seven hopeful landrace genotypes of common bean (Phaseolus vulgaris L.) collected from the region were analysed by SDS-PAGE. The protein bands of the genotypes were scored for their absence and presence and used for genetic distances which were utilised for a similarity matrix anal...

DNA (deoxyribonucleic acid), responsible for cellular metabolic activations until 4.6 billion years ago, which was accepted to be the beginning of life on earth, was not known until the 1900s, and research on the function of the gene began in the 1800s. As a result of Mendeli's work in the 1900s, there was a great explosion of information about the...

Biotechnological Developments in Agriculture

Twelve primers were analysed at ten chicken lines by RAPD named RIR I, RIR II, Barred I, Barred II, Colombian Rock, Line-54, Blue Line, Maroon Line, Black Line, Brown Line provided by Ankara Poultry Research Institute-Turkey. Totally, 35 bands were scored and only 15 bands were showed polymorphism. The highest and lowest genetic similarities were f...

The genetics of plants regenerated from anther-derived callus was investigated using single crosses & inbreds of maize. Anther culture in maize ( Zea mays L.) usually follows an indirect procedure involving callus induction and differentiation on at least two different media. Alternatively, development of a direct procedure by which plantlets can b...

The genetic diversity is an important issue in designing crop improvement programmes, which is also valued for the management of germplasm and for evolving conservation strategies. For determining genetic diversity, molecular markers are the best tools. To date, different marker systems have been used for biodiversity analysis. These include restri...

The study covers the relationship between tobacco genotypes in terms of chromosome homology by pairing configurations and chiasma frequency growing at different areas of the middle black sea region. For this purpose, three genotypes (Samsun, Bafra and Merzifon) and their crosses were studied by acetocarmine stain technique at the stage of metaphase...

Increasing the relationships in domestic plants to wild types, the importance of wild type because of their carried characteristics has been more understood. At the conservation of genetic resources taking place wild types, the information on the amount of the variation, distribution and characterization is important. Gene resources undergo genetic...

A 3 hr. period treatment with a 0.2% aqueous solution of colchicine was given to one week old seedlings of seven inbred lines of wheat ( Triticum aestivum L) plants. Comparisons were made in the CT1 generation between the untreated controls (2x) and colchicine treated plants (C2x) for variation of leaf mesophyll cell size and chloroplast number. Di...

Onion chromosomes ( Allium cepa L.; 2n=2x=16) were analysed for karyotype analysis using aceto-orcein staining technique, and classified due to their lengths from long to short arms whether satellite exists or not.The length of arms appeared to be very close to each other and got more force their identification. The length of long arms showed a var...

Transferrin proteins used in sheep genotypes have been genetically studied. Genetic analysis, crosses of Sönmez x Karayaka (G1), Sakiz x Karayaka, Karayaka x Karayaka, Hempshire Down x Karayaka (F1), (Sakiz x Karayaka (F1)) x (Hempshire Down x Karayaka (F1) in six sheep genotypes and their Genotypes were examined in terms of gender and 15 transferr...

The color-polimorphism at the egg-shell of Japanese quail which was investigated by separating the eggs five groups according to their spot existences on the egg-surface : white, sandy-spotted, little-spotted, medium-spotted and high spotted levels of the eggs with the gene character of chromosome locus. The general specifications of the eggs were...

The Exchange of unfertilised egg cell nucleus (n) with somatic cell nucleus (2n) is known as genetic cloning or copying. In decades, the genetic cloning taking place an important role on the science World put forward different perspectives on the production of plants and animals. In this revieew, it was considered to be given the bacground of studi...

The existence of yields handled from farm animals and its related income belongs to offspring yield. Recent years, researchers study on the reduction of loss (20-40%) occuring at prenatal period in order to increase the offspring yiield. These studies imply the genetic factors play a role on the losses and in some animals during DNA replication on...

Transferrin and hemoglobin polymorphism and genetic differentation selection Abstract:Transferrin (Tf) and haemoglobin (Hb) which both take place in blood are proteins carrying Fe and O2 molecules. The Tf from these proteins consists of 16 alleles and Hb includes 2 alleles which show genetic polymorphism and take place in autosomal chromosomes. Alt...

Egg shell quality was examined due to their pigmentation rates on the egg shell in japanese quail. For this purposes, the eggs were classified four groups due to their pigment density and their absorbance were measured by spectrophotometer. The weight lost and shell rate were calculated comparing both of them with the absorbance of protoporphyin an...

Festuca and Lolium species are plants used commonly in meadow-grass production. Both species carry different characters that are important for agronomy and it is considered that the assemble of those characters on the same plant will enable an advantage on their breeding and production. The studies show that the genetic instability in these hybrids...

The chromosome pairing in auto-and allo-tetraploids, affected by homologous degree and genes, genetically seems to start preferentially at the distal regions. If it is assumed that the distal regions is affective on chromosome pairing initiation, the random pairing of multivalents should be 0.667. The lower levels of this random pairing reveals the...

Egg color genes and its quantitative utilisation in japanese quail (Coturnix coturnix japonica). 10th European Poultry Conference. Jerusalem, 21-26 June 1998. Israel (Abstract).

In organisms, recombination is a source of variation so that they can adapt to their environment. The mechanism of recombination is a complex event that occurs between homologous chromosomes. For this reason, chromosome replication is important for the flow of genetic information. We discuss here that synapsis and recombination events are sequentia...

Japanese quail (Coturnix coturnix japonica) population shows a variation in terms of shell color in twı categories; the bachground color from White to yellow and existence of spots with and without ones in addition to feather color. The data were provided from over 2245 eggs by a population for 500 quails of phenotype observations. The gene and gen...