Ahmed Majeed Al-Shammari

Al-Mustansiriya University · Iraqi Center for Cancer and Medical Genetic Research

Glioblastoma multiforme (GBM) is one of the most life-threatening types of cancer that is difficult to treat. The search for effective yet safe therapy is progressing and non-conventional therapies such as using viruses as a smart and selective agent against cancer are promising. The aim of the study was the presence of a reliable method to use New...

In this study, a synthetic scaffold was prepared from polycaprolactone (PCL) and polyurethane (PU) blend, in a ratio of [2:1] [PCL: PU], using electrospinning technique. Electrospun scaffolds from native PCL and PU were also prepared for comparison, using the same polymer concentration 15% weight/ volume w/v. The detailed microstructure and other p...

Turkish inventory patent about closed method for SVF extraction using ultrasound waves

Objective: Breast cancer is one of the most lethal diseases in women, both worldwide and in Iraq. The high mortality rate is attributed primarily to the chemo resistance to conventional therapeutics. The search for effective and safe treatments is critical. One promising agent that has shown activity against various cancer types is retinoic acid (R...

Neural stem cells (NSCs) are progenitor cells which have the ability to self-renewal and potential for differentiating into neurons, oligodendrocytes, and astrocytes. The in vitro isolation, culturing, identification, cryopreservation were investigated to produce neural stem cells in culture as successful sources for further studies before using it...

Gold nanoparticles loaded TNF-α and CALNN peptide as a drug delivery system and promising therapeutic agent for breast cancer cells, Materials Technology, ABSTRACT We investigated the anti-cancer properties of gold nanoparticles loaded TNF-and CALNN peptides, which we proposed as a potential drug delivery system using in vitro and in vivo models. T...

Oncolytic virotherapy is one of the emerging biological therapeutics that needs a more efficient in vitro tumor model to overcome the two-dimensional (2D) monolayer tumor cell culture model's inability to maintain tissue-specific structure. This is to offer significant prognostic preclinical assessment findings. One of the best models that can mimi...

Glioblastoma multiforme (GBM) is considered to be one of the most serious version of primary malignant tumors. Temozolomide (TMZ), an anti-cancer drug, is the most common chemo-therapeutic agent used for patients suffering from GBM. However, due to its inherent instability, short biological half-life, and dose-limiting characteristics, alternatives...

Digestive system tumors, including esophageal carcinoma considered one of the fatal tumors with a high mortality rate. The search for effective therapeutics is necessary to improve cancer patients' survival. The classical cancer therapy showed many health problem during long time treatment, therefore alternative medicine needful to improve cancer t...

Cancer cells are distinguished by enhanced glucose uptake and an aerobic glycolysis pathway in which its products support metabolic demands for cancer cell growth and proliferation. Inhibition of aerobic glycolysis is a smart therapeutic approach to target the progression of the cancer cell. We employed acarbose (ACA), a particular alpha-glucosidas...

Cytokine storm is one of the causative deaths in a patient with severe acute respiratory syndrome. is study aimed at evaluating the prophylaxis e ect of quercetin complexes with zinc and bu ered ascorbic acid upon cytokine storm induction in mice and identifying the complex's acute toxicity. Mice were randomly divided into three groups: group A, co...

Purpose: This study investigated periodontal ligament (PDL) restoration in osseointegrated implants using stem cells. Methods: Commercial pure titanium and zirconium oxide (zirconia) were coated with beta-tricalcium phosphate (β-TCP) using a long-pulse Nd:YAG laser (1,064 nm). Isolated bone marrow mesenchymal cells (BMMSCs) from rabbit tibia and...

Objective: This study aimed to the preparation of stable colloidal silver nanoparticles (AgNPs) and test the novel combination therapy, which comprises AgNPs and lives attenuated measles virus (MV) vaccine, to target breast cancer cells. the safety of the proposed therapy in normal breast epithelial cell lines (HBL-100) was tested. Methods: Silver...

Oncolytic virotherapy is one of the promising cancer therapies approaches. It shows augmented killing effect through combination therapy by attacking cancer cells across multiple mechanisms of action to overcome cancer cells resistance. In previous studies by our group, we noticed a synergistic anti-tumor effect when combining cobalt ferrite (CoFe2...

Cancer cells heavily utilise angiogenesis process to increase vascularisation for tumour mass growth and spread, so targeting this process is important to create an effective therapy. The AMHA1 strain of Newcastle disease virus (NDV) is an RNA virus with natural oncotropism. NDV induces direct tumour cytolysis, apoptosis, and immune stimulation. Th...

A characteristic of cancer cells is increased glucose uptake and glycolysis for energy production and hydroperoxide detoxification due to mitochondrial dysfunction. Thus, inhibition of glucose uptake and glycolysis represent smart novel therapy. We used 2-deoxyglucose (2DG) as a glycolysis inhibitor and acarbose (ACA), a specific alpha-glucosidase...

Alot of medical and industrial applications used the metal nanoparticles (NPs) with increase interest to be used as cancer therapy. The current work aimed to prepare AuNPs and AgNPs through the use of plasma jet and test their antitumor mechanism of apoptosis induction. The results indicating the face-centered cubic structures and crystalline natur...

Objective(s): This study aimed to prepare a stable colloidal silver nanoparticles (AgNPs) and assess its novelty combination therapy, which comprises AgNPs and lives attenuated Measles virus (MV) vaccine, to target breast cancer cells. The safety of the proposed therapy in normal breast epithelial cell lines (HBL-100) was evaluated. Methods: Silver...

Digestive system cancers are common primary cancer in humans. Plants are an excellent source of compounds used in the treatment of different types of cancer. Virotherapy is a promising tool for fighting cancer because of its safety and selectivity. Thus, the present study proposed a new combination therapy consisting of secondary metabolite compoun...

This is the first study to report on the isolation of bovine leukemia virus (BLV) from peripheral blood mononuclear cells of two cross bred cows in Iraq. The cattle were seropositive by ELISA when selected while being surveyed for the detection of BLV. Among six cows, two were cases of persistent lymphocytosis (PL). Cytopathology was characterized...

Background: Breast cancer has been the highest-ranked malignancy among the Iraqi population since 1986. Althaea officinalis is a perennial plant and native to Iraq and Asia, Europe and United States of America.Objective: To evaluate the cytotoxicity of Althaea officinalis extracts on AMJ13 breast cancer cell line.Methods: The cytotoxic activity of...

Three-dimensional spheroids of cancer cells conceder vital tools for study cancer drug screens and cancer cell biology. Because of that type of culture system have a specific ability to mimic many aspects of the in vivo conditions of cancer cells. The spheroid culture conditions can summarize the physicochemical gradients in cancer cells, including...

Biological therapy is opening up a new age for the treatment of cancer. The bacteria -cancer interaction was firstly observed by Busch and Fehleisen (1). To reducing the potential of pathogenicity of using active bacterial, using bacterial products like proteins, enzymes, immune-toxins, that straightaway target malignancy cells and regress the volu...

Newcastle disease virus (NDV) is promising antitumor agent that triggers immediate cytolysis of the tumor, apoptosis and activation of the immune system. This study was aimed at testing the antiangiogenic NDV Performance on a model of breast cancer. NDV has been studied against mammalian adenocarcinoma AN3 transplanted into syngeneic immunocompeten...

Transdifferentiation means mature cell conversion into other mature cells. Ethical issues, epigenetic failure, or teratoma development are found in cellular reprogramming strategies. Thus, new methods are needed. This study aimed to develop a new novel formula of chemical molecules and growth factors that differentiate skin fibroblasts into insulin...

Angiogenesis is the physiological process through which new blood vessels generate from pre-existing vessels and it acquired therapeutic value in cancer treatment because malignant tissue required the formation of new blood vessels to grow and metastasize. Bevacizumab is a recombinant humanized monoclonal antibody. It is the first angiogeneic inhib...

One of the "hallmarks of cancer" is altered energy metabolism, which is increased glycolysis in cancer cells, the primary source of energy that uses this metabolic pathway to generate ATP. Oncolytic virotherapy with aerobic glycolysis inhibitor smart therapeutic approach to induce apoptosis in cancer cells. The current study aimed to use the 2-Deox...

The objective of this study was identifying the flavonoids in Rhizomes of Iraqi Rheum ribes and utilization these extract to phytosynthesis of cobalt ferrite nanoparticles with characterization of them. The results obtained from the HPLC analysis indicate that rhizomes of Iraqi R. ribes contain seven compounds of flavonoids which include: 1.735 μg/...

Lentogenic LaSota strain, attenuated AMHA1 strain and AMHA2 virulent Iraqi strain of Newcastle disease virus (NDV) are three replication-competent, non-recombinant natural oncolytic Newcastle disease viruses evaluated in the current study for their anticancer effects against breast cancer cells. All these strains selectively replicate within tumor...

Esophageal cancer is very aggressive and the 6 th most prominent cause of cancer death throughout the world. It had an extremely poor prognosis, and current chemotherapy treatments like 5-fluorouracil (5-FU) often have limited success and fatal outcomes. Recently, plenty of studies report the significant anti-proliferative effects of different extr...

In the current study, polyethylene glycol (PEG) was employed to cap super magnetite nanoparticles (Fe3O4 NPs) through hydrothermal preparation. The main goal of this study is to investigate the infuence of physical incorporation of polyethylene glycol (PEG) loaded Fe3O4. The anticancer potentials of these particles were studied against breast cance...

In this study, the prevalence of diverse bovine papilloma viruses in cutaneous lesions was investigated in Iraqi cows. Fourteen specimens were taken from udder growths of Iraqi dairy cows, which demonstrated rice grain-like and small, raised flesh-like appearance. DNA was extracted from the teat wart samples, then was amplified by the polymerase ch...

The unprejudiced of this education is to gauge the ability of the retinoic acid to induce apoptotic cell death in hematological tumors through caspase dependent or independent apoptotic pathway, The cytotoxicity effects of retinoic acid of different concentrations (400,350,300,250,200,150,100,50,25,12.5 µg\ml) and exposure for all hematological mal...

Cancer cells are mainly dependent on glycolysis to generate Adenosine Triphosphate (ATP) and intermediates required for cell growth and proliferation. Inhibition of glycolysis might be of therapeutic value in antitumor treatment. We investigated the effects of D-mannoheptulose (MH) as the inhibitor of hexokinase on glycolysis metabolism and apoptos...

The methanolic extract of Conocarpus erectus was tested in vitro on cancerous cell lines AMJ, MCF7, Cal51, and MDMBA and normal mouse embryonic fibroblast (MEF) cell line as a comparison. Morphological study for exposed cells was done by examination under phase-contrast inverted microscope. High-performance liquid chromatography (HPLC) was used to...

Background: Linalool is a monoterpene compound with various potential therapeutic applications in several medical fields. Previous studies have indicated the activity of linalool against cell lines; however, its high level of toxicity restricts its use. The aim of this study was to design and manufacture compounds with a novel structure that can b...

Newcastle Disease Virus (NDV) can modulate cancer cell signaling pathways and induce apoptosis in cancer cells. The laboratory-based studies of the oncolytic NDV requires a reliable protocol for the propagation of the oncolytic NDV. A comprehensive protocol is provided for virus propagation in fertile chicken eggs, which consistently yields high ti...

Background: Most cancer cells exhibit increased glycolysis and use this metabolic pathway cell growth and proliferation. Targeting cancer cells' metabolism is a promising strategy in inhibiting cancer cell progression. We used D-Mannoheptulose, a specific hexokinase inhibitor, to inhibit glycolysis to enhance the Newcastle disease virus anti-tumor...

Objectives The strategies of tissue-engineering led to the development of living cell-based therapies to repair lost or damaged tissues, including periodontal ligament and to construct biohybrid implant. This work aimed to isolate human periodontal ligament stem cells (hPDLSCs) and implant them on fabricated polycaprolactone (PCL) for the regenerat...

Newcastle disease virus (NDV) can modulate cancer cell signaling pathway and induce apoptosis in cancer cells. Cancer cells increase their glycolysis rates to meet the energy demands for their survival and generate ATP as the primary energy source for cell growth and proliferation. Interfering the glycolysis pathway may be a valuable antitumor stra...

Hepatocellular carcinoma (HCC) globally ranked fifth common cancer and the third-leading cause of death. This study aimed to characterize the new mouse hepatocellular carcinoma cell line (HCAM) for some of the most important proteins involved in cell cycle regulation P53, HER2/neu, and EGFR by immunocytochemistry. Also, to measure the sensitivity o...

Background: Calendula officinalis L used in Iraqi folklore medicine for several medical applications. This research evaluated the leaves extract as an anti-breast cancer agent in in-vitro cancer cell line systems and studies its active compounds. Crystal violet viability assay was used to determine the cytotoxicity of the leave methanolic extract o...

Background Cold atmospheric plasma (CAP) is widely used in the cancer therapy field. This type of plasma is very close to room temperature. This paper illustrates the effects of CAP on breast cancer tissues both in vivo and in vitro. Methods The mouse mammary adenocarcinoma cell line AN3 was used for the in vivo study, and the MCF7, AMJ13, AMN3, a...

Sixty-four isolate were klebsiella pneumoniae. Fourteen bacteria isolates "Kelbsiella species" were taken from soil and water hospital in the period between October to December 2018, those isolated were cultured on a blood agar to test their ability to hydrolytic due to formation the inhibition zone. Twenty one isolates of K. pneumoniae were select...

A new liver cancer cell line (HCAM) has been characterized; it is considered as a useful tool in liver cancer research. The HCAM cell line was established from the primary tumor of a white male mouse with spontaneous hepatocellular carcinoma. The cells were morphologically characterized by light microscopy and found to be single polygonal epithelia...

Skin is an important and complex organ that is composed of epidermis and dermis. Fibroblasts are mesenchymal cells which found in connective tissues of dermis. These cells have many features and characteristics which are widely used for treatment of various diseases. So, the present study aimed to isolate and culture of fibroblast which is used as...

The common type of liver cancer is hepatocellular carcinoma, which often emerges in the setting of major chronic liver disease and has a poor prediction. This study aimed to characterize the cell line of hepatocellular carcinoma established from mice infected with liver cancer. The cell lines characterization was well-defined by morphology, growth...

tagged (GFP and Luciferase) AdVs on breast cancer cell lines with distinctive characteristics (i.e. hormone sensitive, HER2/neu, triple negative) and breast epithelial cells. For this project 20 different reporter gene tagged adenovirus types were screened with respect to their transduction rates measured by the expression of the luciferase enzyme...

Breast cancer is worldwide health trouble takes over 40,000 lives of women per year in the U.S. only and the first cause of death from cancer in females in Iraq. Newcastle disease virus has an old history of being used in oncotherapy explore in several presymptomatic fields. Here, this work was conducted to determine the cytotoxicity and apoptosis...

Background The frequent outbreaks of Newcastle disease virus (NDV) in Iraq pose a constant threat to commercial poultry, despite the introduction of routine vaccination programmes. Several factors, particularly stress factors and coinfections, might play a role in increasing NDV outbreaks in poultry species. Objectives The current study was aimed...

Galangin is a flavonoid from the root of Alpinia officinarum. It has novel anticancer properties. In the current study, ovarian cancer cells SKOV3 were treated with galangin alone, galangin combined with Gold nanoparticles (AuNPs) and AuNPs alone, and cytotoxic activity of these agentswas investigated using MTT viability assay. The expression of ap...

Background: Adipose derived-mesenchymal stem cells have been used as an alternative to bone marrow cells in this study. Objective: We investigated the in vitro isolation, identification, and differentiation of stem cells into neuron cells, in order to produce neuron cells via cell culture, which would be useful in nerve injury treatment. Method: Mo...

Background: One of the world's leading causes of death among females is breast cancer. Oncolytic viruses are promising anticancer therapy that can overcome resistance to current conventional therapies. Measles virus replicates in and destroys malignant cells without affecting healthy cells. The study aimed to evaluate the lives attenuated Measles...

Phytotherapy has been used to treat a different type of diseases including cancer for a long time, and it was a source for different active anti-tumor agents. Oncolytic Newcastle disease virus (AMHA1) are very promising anti-tumor therapy. Nevertheless, NDV-based monotherapeutics have not been very useful to some resistant tumors. Thus, the efficie...

Newcastle disease virus (NDV) is a wide-spectrum anti-tumor agent. The oncolytic selectivity of NDV, a family of Paramyxoviridae, depends on the differential type of inducing different death pathways. This work was conducted to further understand the oncolytic effect of LaSota strain. A mouse breast cancer model (Murine mammary adenocarcinoma cell...

Newcastle disease virus (NDV) is a wide-spectrum anti-tumor agent. The oncolytic selectivity of NDV, a family of Paramyxoviridae, depends on the differential type of inducing different death pathways. This work was conducted to further understand the oncolytic effect of LaSota strain. A mouse breast cancer model (Murine mammary adenocarcinoma cell...

Background: Plantago lanceolata L. is used in Iraqi folklore medicine to treat injuries, and its extract is prescribed by some herbalists for cancer patients. This research aimed to evaluate the effect of P. lanceolata leaf extract on breast cancer cell lines in vitro and to identify its active compounds. Crystal violet viability assay was used to...

Background:The incidence of cancer is increasing worldwide. Xanthium strumarium may possess anticancer activity, the plant extract for in vitro anticancer activity against a panel of three human cell lines (Breast MCF7, Renal TK10 and Melanoma UACC62), exhibit anticancer activity against these three human cell lines screened by National Cancer Inst...

Most cancer cells exhibit increased glycolysis and use this metabolic pathway for generation of ATP as the main source of cell growth and proliferation. Targeting cancer cells metabolism is a promising strategy in inhibiting cancer cells progression that is known to exhibit increased aerobic glycolysis. We used glucose hexokinase inhibitor (D-Manno...

Hematological malignancies remain one of the leading causes of death worldwide despite advances in cancer therapeutics. Newcastle disease virus (NDV) is a member of Paramyxoviridae that elicits considerable interest as an anticancer agent because it can replicate up to 10 000 times faster in human cancer cells than in most normal cancer cells. Seve...