Ahmed aboul-Enein

• Professor
• Cairo University

The bio-insecticide spinosad (SPD) is increasingly being used in pest management programmes. In order to further assess its toxic effects to non-target species, male rats were exposed sub-chronically to SPD at a dose equivalent to (1/20) LD50 for four weeks. In order to assess the toxicity of SPD, parameters such as the activities of enzymes and co...

Salvia rosmarinus, which belongs to the Lamiaceae family, is recognized for its extract antioxidant, anti-inflammatory, and anti-cancer properties. The present work was focused on the anticancer and genotoxicity against prostate cancer (PC3), therefore, it was designed to evaluate plant characters considered as risk factors for cancer including ant...

We provide proof-of-concept for the innovative method of 2,6-dichloroindophenol (DCPIP) for measuring the antioxidant activity of plant extracts. Antioxidant content can be determined using the standard DCPIP test and compare the results with the DPPH results as conventional method. DCPIP operates on the premise that the deep blue color of the oxid...

Mango (Mangifera indica) and guava (Psidium guajava) seeds have several pharmaceutical applications and biological activities because as they have been recognized with different bioactive molecules (phenolic compounds) such as flavonoids, phenolic acids, and catechins, so they have antioxidant and anticancer activities. The aim of the present study...

This article reports on the main constituents of Hyssopus officinalis and Perilla frutescens essential oils (EOs) and their activity as antioxidant, antimicrobial, anti inflammatory and anticancer. The major component of H. officinalis essential oil was isopinocamphone followed by pinocamphone, β-pinene, β-phellandrene, α-thujene and bicyclo-germac...

Genus Lavandula belongs to Lamiaceae family containing almost 40 different species, many hybrids, and nearly 400 registered cultivars (Upson and Andrews 2004). Mane folk uses and biological activities of Lavandula species are reported in addition to their uses in the pharmaceutical and other industries benefits. The biological activities of Lavandu...

Organic fractions and extracts of willow (Salix safsaf) leaves, produced by sequential solvent extraction as well as infusion and decoction, exhibited anticancer potencies in four cancerous cell lines, including breast (MCF-7), colorectal (HCT-116), cervical (HeLa) and liver (HepG2). Results of the MTT assay revealed that chloroform (CHCl3) and eth...

Although the safety of food additives had been assessed individually, these permitted additives may be unsafe if used together; this study was piloted to assess the safety of various food additive mixtures. Fifty male Albino rats-Wistar strain (4 weeks old) were distributed into 10 groups, the first group orally administered distilled water, the ot...

Targeted drug delivery offers great opportunities for treating cancer. Here, we developed a novel anticancer targeted delivery system for piperine (Pip), an alkaloid prodrug derived from black pepper that exhibits anticancer effects. The tailored delivery system comprises aggregated hydroxyapatite nanoparticles (HAPs) functionalized with phosphonat...

The effect of peels (kiwi, banana and leaves (olive and banana)acetone extract 80%on streptozotocin (STZ) induced diabetic rats was investigated. Oral administration of 500 mg crude extract /kg body weight for 30 days post induction of diabetes, showed remarkable improvement in all biomarkers under investigation compared to standard drug. Olive lea...

Background and purpose Glioma is one of the most aggressive primary brain tumors and is incurable. Surgical resection, radiation, and chemotherapies have been the standard treatments for brain tumors, however, they damage healthy tissue. Therefore, there is a need for safe anticancer drug delivery systems. This is particularly true for natural prod...

The effect of acetone extraction of peels and leaves of banana and peels of kiwi as well as olive leaves on streptozotocin (STZ)-induced diabetic rates. Oral ingestion of their extracts for 60 days reversed the adverse effects of diabetes as compared to control rats. Olive leaves showed the highest percentage of improvement in diabetic rats, while...

The present investigation evaluated 4 distinct solvent compositions for their proportional capacity to extract total phenolic and total flavonoid (TF) and total tannins (TT) components of the peels of kiwi (Actinidia Deliciosa Planch) as well as to profile the composition of these plant by-product and to measure their antioxidant capacity, antimicr...

A powerful and applicable milk-clotting enzyme was extracted from the different wild Egyptian plant Silybum marianum parts. Seeds extract was the most efficient and exhibited the highest milk-clotting activity and the lowest proteolytic action. Partial purification of the enzyme seeds extract was performed by sequential and batch precipitation by d...

Leaf extracts of Cassia alata L traditionally used for treatment of a variety of diseases. Chloroform fraction of leaves was evaluated for its potential antitumor properties in vitro. MTT assay was used to examine the cytotoxic effect on three human cancer cell lines namely HepG2, MDA-MB-231 and Caco2. Chloroform fraction showed remarkable cytotoxi...

Background: Methylene tetrahydrofolate reductase (MTHFR) C677T polymorphism was reported as a genetic variant in liver steatosis and fibrosis. This is a study of the association between MTHFR C677T polymorphism and HCV core with severity of steatosis in HCV GT4 patients. Methods: 111 HCV patients and 112 control subjects were recruited. Polymorp...

This study aims to investigate the major constituents of hexane fraction, of Calliandra haematocephala (Hassk.) leaves. In addition to, the hepatoprotective and antioxidant activities of the total alcohol extract against carbon tetrachloride (CCl4) -induced liver damage in vivo. C. haematocephala leaves were extracted with methanol, and hexane, fra...

This study was carried out to investigate the chemical composition and biological activity of banana peel extracts; the efficiency of the different solvent systems: aqueous, 80 % methanol, 80% ethanol and 80% acetone was used for extraction of phenolic, flavonoid and tannin compounds. Banana peel relative antioxidants potential by four assays DPPH...

Background Eichhornia crassipes (Mart) solms is an invasive macrophyte causing serious problems to the network of irrigation and drainage canals in the Nile Delta region. The present study aim to evaluate the potential anticancer and antioxidant activities of Eichhornia crassipes crude extract and its pure compounds. Methods The macrophyte was col...

The fungus, Emericella nidulans was isolated from soil. The ITS region of 5.8S rRNA of the isolated fungus was amplified and sequenced. E. nidulans EGCU312 was given an accession number: KC511056 in the NCBI GenBank. Twenty one (21) fractions were obtained from the ethyl acetate extract of fungal filtrate. Fraction no. 12 showed the highest antioxi...

The present work discusses the separation and purification of bioactive compounds from the crude ethanolic extracts of Cassia italica and Solanum nigrum leaves. Identification of compounds in the crude extract was accomplished using different spectrophotometric analyses (MS, NMR and IR). Eight active compounds were identified from crude extract of...

Natural substances offer interesting pharmacological perspectives for antiviral drug development with regard to broad spectrum antiviral properties and novel modes of action. Drugs currently used to treat cutaneous or genital herpetic infections are effective in limiting disease, but the emergence of drug-resistant viruses in immunocompromised indi...

Supplementary materials aims to provide more details of drug target database which was used for analysis as well the toxicities predicted for natural products, list of targets identified by Ontomine and list of cancer related proteins used for forward docking to natural products.

The aim of the analysis of just 13 natural products of plants was to predict the most likely effective artificial mixtures of 2-3 most effective natural products on leukemia cells from over 364 possible mixtures. The natural product selected included resveratrol, honokiol, chrysin, limonene, cholecalciferol, cerulenin, aloe emodin, and salicin and...

An efficient multiple shoot has been developed for three varieties of Echinacea purpurea using shoot tip explant. Multiple shoots originated when shoot tip cultured on MS-medium supplemented with 0.5 mg/L BA and different concentrations of NAA. Optimum shoot multiplication was observed on MS-medium containing 0.5 mg/L BA for the three varieties. Th...

A powerful and applicable milk-clotting enzyme was extracted from the wild Egyptian plant Silybum marianum seeds. Partial purification of the enzyme seeds extract was performed by sequential and batch precipitation by different precipitating agents, i.e. ammonium sulphate, ethanol and acetone. The enzyme fraction precipitated at 50% batch ammonium...

Plants have played a significant role in the treatment of cancer and infectious diseases for the last four decades. Natural products have been rediscovered as important tools for drug development despite advances in combinatorial chemistry. Egyptian flora, the most diverse in the world, has become an interesting spot to prospect for new chemical le...

Water hyacinth, Eichhornia crassipes (Mart) Solms, originating in the amazonian basin, is a warm water aquatic plant. Water hyacinth is considered one of the most productive plants on earth and, accordingly, is considered one of the top 10 world's worst weeds. Water hyacinth spread to other tropical and subtropical regions by humans. It invaded abo...

The present study was aimed at developing an efficient protocol for callus induction from the leaves of Moringa oleifera and to investigate its crude extract antileukemia activity on leukemia cells. Several secondary metabolites are present in M. oleifera as the plant serves as reservoirs for various bioactive compounds. Callus cultures of M. oleif...

Medicinal plants are important elements of indigenous medical system that have persisted in developing countries. Many of the pharmacological principles currently used as anticancer agents were first isolated from plants. However, some important anticancer agents are still extracted from plants because they cannot be synthesized chemically on a com...

Shoot tips were excised from D. lanata seedlings and cultured on MS-medium supplemented with different concentrations of naphthalene acetic acid (NAA) and kinetin (Kin) to obtain in vitro plants. Evaluation of the content of digitoxin and digoxin in the produced in vitro and in vivo plants (2-months old) were estimated. Moreover, the effect of diff...

Organochlorine and Organophosphorus Pesticides are found in many parts of the environment in small concentrations, but they have cumulative characteristics thus become a threat to human health and life. This study is focused on detection and determination of some of these pesticides in the muscles of chicken and bovine in the Egyptian market whethe...

In recent years there has been an increasing interest for application of natural products as antiinfectives and concerns about the safety of synthetic compounds have encouraged more detailed studies of natural resources. Two different strains of the nontoxic cyanobacterium Arthrospira from the United States and Egypt have been characterized by sequ...

Natural products from plants are rich sources of chemical diversity and most of the pharmacologically active principles currently used as drugs, including anticancer agents are plants products. Egypt, a semi-arid region has abundant plants resources, which are traditionally used for various disorders. The aim of the present study was to evaluate so...

The aim of this study was to evaluate the potential anticancer properties and modulatory effect of selected Aloe vera (A. vera) active principles on antioxidant enzyme activities. Thus, three anthraquinones (Namely: aloesin, aloe-emodin and barbaloin) were extracted from A. vera leaves by supercritical fluid extraction and subsequently purified by...

Fast-growing normal root culture of Vernonia amygdalina, a potent African medicinal plant was established from leaf explants of in vitro-raised shoot induced from the stem nodal segments on Murashige and Skoog (MS) medium containing 0.5 mg L” 6benzylaminopurine (BA) in combination with 0.5 mg L! a-naphthalene acetic acid (NAA). ). Explants were cul...

Vernonia amygdalina, Del (Compositae) is an African medicinal plant well known for producing the anticancer agents' vernodaline and vernolide. It grows wild under severe anthropogenic and environmental pressures. The roots are the principle material for herbal medicine. It is collected from the wild there is great variability in the quality and eff...

Calcium (Ca(2+)) transients have been shown to take place in response to diverse developmental and physiological cues. Also, it is involved in biotic and abiotic stress signaling. Nitric oxide (NO) is an important signaling molecule that plays a crucial role in plant growth and development, starting from germination to flowering, ripening of fruit...

To investigate the transcriptional regulation of gene expression, chimeric fusions, between the beta-glucuronidase reporter gene (GUS) and the isolated promoter regions of the pvPDF gene (pvPDF-PRO: GUS), were constructed and introduced into Nicotiana tabacum. Analysis of transgenic pvPDF-PRO:GUS tobacco plants indicated that GUS activity was obser...

In this study the molecular characterizations of Chicory and Jerusalem artichoke plants was determined using random amplified polymorphic DNA (RAPD) technique, the protein profile using SDS' PAGE technique and isoenzymes detection. The antibacterial activity of water extract of two plants against seven different strains of bacteria was also evaluat...

Many higher plants contain novel metabolites with antimicrobial, antifungal and antiviral properties. However, in the developed world almost all clinically used chemotherapeutics have been produced by in vitro chemical synthesis. Exceptions, like taxol and vincristine, were structurally complex metabolites that were difficult to synthesize in vitro...

1 Corresponding author's e-mail: ABSTRACT A water culture experiment was conducted with two-cucumber cultivars (Cucumis sativus L.) Cv. named Dotto (referred as cultivar I) and Beit alpha (referred as cultivar II) to investigate the differences between both cultivars in their capabilities to release root exudates (phenolic compounds, soluble sugars...

The young developing leaves of willow (Salix safsaf, Salicaceae) trees have antileukemic activity. After a 24-h incubation in vitro, the crude water extracts of the leaves killed a majority of the blasts of acute myeloid leukemia (AML, 73.8%).

The antioxidant of seven algal extracts was evaluated by decrease the thiobarbituric acid reactive substance (TBARS) produced from lipid peroxidation of liver microsomes induced by Fe<SUP>++</SUP>/ascorbate, Fe<SUP>++</SUP>/H<SUB>2</SUB> O<SUB>2 </SUB>and CCl<SUB>4</SUB> model systems. All algal extracts had significant effect to prevent the produc...

Dunaliella salina a β-carotene accumulating halotolerant algae has been analyzed for the effect of various growth conditions on its antioxidant vitamin contents β-carotene, tocopherol and ascorbic acid. Vitamin contents of Dunaliella salina grown in culture contained sufficient nitrogen (70 ppm N ) and NaCl ( 10 %) under optimum light intensi...

-Galactosidase (E.C.3.2.1.22) from Penicillium janthinellum was purified by precipitation and fractionation with ammonium sulphate, cold acetone or ethanol, calcium phosphate gel, and column chromatographies on Sephadex G-100 and G-200. The enzyme was purified about 110.39-fold when Sephadex G-100 was used. -Galactosidase exhibited the optimum pH a...

Abstract— Single-strand break formation and biological deactivation of plasmid pBR322 DNA in the presence of tris(2,2′-bipyridyl)-ruthenium(II), Ru(bpy)2/3;+, and K2S2O8, upon irradiation with visible light(400–500 nm), were studied in aqueous solution at room temperature. Conditions of complete binding of Ru(bpy)2/3;+ to the strand were employed....

Garlic bulbs ( Allium sativum ) were extracted with distilled water or ethanol. The extracts were then incubated with EHRLICH ascites carcinoma cells at 37 C for 1 h. These pretreated cells were injected into swiss albino mice which survived over 12 weeks. To the contrary. tumor cells which were pretreated with garlic extracts, produced ascites tum...

The hyperlipemia and hypercholesterolemia were induced in mice by injection (i.p.) of a single dose of Triton WR‐1339 at a dosage of 900 mg/kg body weight. These animals were characterized by significant increases in the average levels of plasma total lipids, cholesterol, phospholipids and triglycerides. The highest rise of each fraction was after...

A diversity of actinomycetes was isolated from soil samples obtained from five governorates of Egypt (El&Behira, Tanta, Giza, Kafer El&Sheekh and Alexandria). The s oil samples were mainly collected during the expedition (15& 25 October 2007). A total of 12 isolate of actinomycetes, probably mostly Streptomyces, were obtained from the& se different...