Ahmed S Abdulamir

Ahmed S Abdulamir
Al-Nahrain University · department of Microbiology

PhD

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132
Publications
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Publications

Publications (132)
Article
Coronavirus disease 2019 (COVID-19) is a recent respiratory infection caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with serious complications, severe acute respiratory syndrome (ARDS), cytokine storm, and coagulopathies. Complete blood count (CBC) is a routine inexpensive and easy test that provides information regardi...
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Background: Warfarin is the most widely used oral anticoagulant for the prevention and treatment of thromboembolic disorders. Because of narrow therapeutic index and various genetic and non-genetic factors that influence the disposition of the drug, its dose undergoes a great variability. The aim of this study was to determine the allelic variants...
Preprint
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Objectives COVID-19 patients suffer from the lack of curative therapy. Hence, there is an urgent need to try repurposed old drugs on COVID-19. Methods Randomized controlled study on 70 COVID-19 patients (48 mild-moderate, 11 severe, and 11 critical patients) treated with 200ug/kg PO of Ivermectin per day for 2-3 days along with 100mg PO doxycyclin...
Article
The current COVID-19 pandemic needs unconventional therapies to tackle the resulted high morbidity and mortality. Convalescent plasma is one of the therapeutic approaches that might be of benefit. Forty nine early-stage critically-ill COVID-19 patients residing in Respiratory Care Units (RCU) of three hospitals in Baghdad, Iraq, were included: 21 r...
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Carnosol, a phenolic diterpene, is one of the effective anticancer agents naturally occurring in rosemary, sage, parsley, and oregano. The chemoresistance problem increased with the routinely used chemotherapy. Therefore, the efforts to find a substitute with safe and low cost have become crucial worldwide. The current study attempts to inspect the...
Preprint
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Objectives The current COVID-19 pandemic needs unconventional therapies to tackle the resulted high morbidity and mortality. Convalescent plasma is one of the therapeutic approaches that might be of benefit. Methods Forty nine early-stage critically-ill COVID-19 patients residing in RCU of three hospitals in Baghdad, Iraq were included, 21 receive...
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Novel Coronavirus, COVID-19 discovered in December, 2019 in Wuhan, China started a world-wide epidemic. It is not clear till now what is the pathogenesis of this virus infection in human or the exact strategies of host immune response in combating this novel threat to human beings. However, morbidity and mortality of COVID-19 infections vary widely...
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Background: The recent emergence of multidrug resistance (MDR) in Acinetobacter baumannii (A. baumannii ) has raised concern in health care settings in Iraq. This is the first report of the whole genome sequence of A. baumannii ZQ isolated from Iraqi patients. To better comprehend the repertoire of MDR genetic elements and organization, we compared...
Article
Breast cancer is the most frequent malignant disease affecting women in the worldwide. Although significant developments have taken place in treatment strategy, tamoxifen remains the standard therapeutic option for breast cancer therapy and for primary prevention of metastatic disease. In breast cancer patients, the most commonly used serum tumor m...
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ABSTRACT Breast cancer is the most frequent malignant disease affecting women in the worldwide. Although significant developments have taken place in treatment strategy, tamoxifen remains the standard therapeutic option for breast cancer therapy and for primary prevention of metastatic disease. In breast cancer patients, the most commonly used seru...
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Objective(s) Phage therapy is a potential alternative treatment for infections caused by Acinetobacter baumannii, a significant nosocomial pathogen, which has evolved resistance to almost all conventional antimicrobial drugs in poor hygiene and conflicts areas such as Iraq. Materials and Methods Bacteriophages were isolated to highly resistant iso...
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Introduction: There is a need for a reliable, time-saving, and specific detection assay for coliforms, environmental E. coli, and Enterobacteriaceae worldwide. Aim: To innovate a new principle of phage-based rapid diagnostic test for detecting E. coli bacteria in short time and low titer. Materials and Methods: A phage mixture of 200 E. coli specif...
Patent
The present invention relates to an anticancer composition for treating cancer. The anticancer composition comprises of an active ingredient having an effective amount of Vigna radiata L. extract. The Vigna radiata L. extract having anticancer activity against cervical cancer cell and hepatocarcinoma cell. The Vigna radiata sprout.
Patent
The present invention relates to an antimicrobial composition for use as antiviral, antibacterial and antifungal agent from natural product. The composition comprises an active ingredient with an effective amount of Vigna radiate L. extract.
Patent
The present invention relates to an antiviral composition for use as antiviral agent from natural product. The composition comprising an active ingredient with an effective amount of Brassica oleracea L. extract. The composition is having an antiviral ability against herpes simplex virus type 1 (HSV-1) and also respiratory syncytial virus (RSV).
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Abstract: To explore the impact of different patient-specific factors on the daily warfarin maintenance dose. This is a cross sectional study which was conducted on 80 Iraqi patients of Arab ethnicity. The ethical approval of this study was obtained from the Medical College / Al-Nahrain University and conducted in accordance with the Declaration of...
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Background: Citrus bioactive compounds, as active anticancer agent, have been under focus by several studies worldwide. However, the underlying genes responsible for the anticancer potential have not been sufficiently highlighted. Objectives: The current study investigated the gene expression profile of hepatocellular carcinoma, HepG2, cells aft...
Article
Background: The emergence of multiple drug resistant (MDR) Mycobacterium tuberculosis (M.TB) and extensive drug resistant (XDR) M.TB lay huge burden on TB endemic countries such as Iraq. Objectives: Bacteriophage (phage) therapy can be used as alternative approach to tackle this problem. Patients and methods: Forty isolates of M.TB were cultured fr...
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Background This study assessed novel approach of using highly lytic phages against methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) biofilms with and without biofilm extracellular matrix- disrupting chemical. Method The resultant phage-based control was assessed in relation to the type of...
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New sources for discovering novel antiviral agents are desperately needed. The current antiviral products are both expensive and not very effective. The antiviral activity of methanol extract of mung bean sprouts (MBS), compared to Ribavarin and Acyclovir, on respiratory syncytial virus (RSV) and Herpes Simplex virus -1 (HSV-1) was investigated usi...
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Background This study was conducted to explore new approaches of animal biocontrol via biological control feed.Method White rats were subjected to 140 highly lytic designed phages specific against E. coli. Phages were fed via drinking water, oral injection, and vegetable capsules. Phage feeding was applied by 24 h feeding with 11d monitoring and 20...
Data
Tomato yellow leaf curl virus (TYLCV) is devastating to tomato (Solanum Symptoms Peroxidase polyphenol oxidase RAPD-PCR lycopersicum) crops and resistant cultivars are highly effective in controlling the disease. Breeding programmes aimed at producing TYLCV-resistant tomato cultivars have utilized resistance sources derived from wild tomato species...
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Tomato yellow leaf curl Virus (TYLCV) threatens the production of tomatoes both in Egypt and the world. A virus was isolated from naturally infected tomato and pepper plants, grown in different areas in Egypt. The investigated isolate was characterized using electron microscopy, serology, biological tests, and polymerase chain reaction (PCR) using...
Article
Leukemic cells are hard-to-transfect cell lines. Many transfection reagents which can provide high gene transfer efficiency in common adherent cell lines are not effective to transfect established blood cell lines or primary leukemic cells. This study aims to examine a new class of cationic polymer non-viral vector, PEGylated-dextran-spermine (PEG-...
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This review aims at discussing the role of Salmonella bacteria as anti-cancer agent against solid and semi-solid tumors. In addition, Salmonella bacteria have been used as a successful bacterial vector for cytotoxic moieties, apoptotic inducers, prodrug enzymes, cytokines, chemokines, tumor associated antigens, DNA and RNA antisense or vaccine frag...
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Vascular endothelial growth factor (VEGF) is a potent angiogenic factor involved in angiogenesis-mediated progression of acute myeloid leukemia (AML). Studies have reported the role of soluble form of fms-like tyrosine kinase (sFlT-1) delivery as an antitumor agent by inhibiting VEGF. This study investigates the outcome of delivery of a VEGF165 ant...
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Background The anticancer and immunomodulatory activity of mung bean sprouts (MBS) and the underlying mechanisms against human cervical and hepatocarcinoma cancer cells were explored. Methods MBS cytotoxicity and MBS-induced anticancer cytokines, TNF-α and IFN-β from cancer cells, and immunological cytokines, IL-4, IFN-γ, and IL-10 from peripheral...
Article
To explore new approaches of phage-based bio-process of specifically pathogenic Escherichia coli bacteria in food products within a short period. One hundred and forty highly lytic designed coliphages were used. Escherichia coli naturally contaminated and Enterohemorrhagic Escherichia coli experimentally inoculated samples of lettuce, cabbage, meat...
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Background: Functional food can be a potent source of wide array of biocomonents with antimicrobial activity. We investigated the antibacterial activity of red cabbage (RC) extract on Gram negative and positive ATCC strains. Most intersting, we, for the first time, explored and analysed the complete phenotypic profile of RC-treated bacteria using O...
Article
The present study was conducted to assess the antioxidant properties of ethanol, ethyl acetate, methanol and hexane extracts from leaves of Pereskia bleo using 2,2-diphenyl-1-picrylhydrazyl method (DPPH assay), ferric reducing antioxidant power (FRAP assay) and β-Carotene-Linoleic acid methods. Total phenolic compounds (TPC) and flavonoids of the l...
Article
The delivery technology of gene into cells has been increasingly paid attention for gene therapy and the generation of genetically engineered cells. If it is possible to artificially introduce exogenous genetic materials into cells at a high transfection efficiency by a delivery technology, the technology will give academically, clinically, and pra...
Article
Spray-wash treatments utilizing concentrations (1, 1.5 and 2%) of acetic, lactic, propionic and formic acids, alone or in combination of two acids, were performed to reduce numbers of Salmonella typhimurium and Listeria monocytogenes on meat tissues at 4 1 C. Salmonella typhimurium- and L. monocytogenes-inoculated meat was spray-washed with treatme...
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Varied mixtures of different concentrations 1, 1.5 and 2% of acetic (AA), lactic (LA), propionic (PA) and formic (FA) acids at 1:1 ratio were spray-washed on inoculated meat to evaluate their efficacy in reducing loads of Escherichia coli O157:H7 andStaphylococcus aureus on meat tissue. It was found that increasing the concentration of the used org...
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The ever rising resistant bacteria and fungi resulted in finding novel antimicrobial sources and agents. Studies confirmed that mung beans have increased phenolic compounds and enhanced defenses during germination. We hypothesized that antimicrobial activities might be found in sprouts of mung beans (MBS), or Vigna radiata (L.) R. Wilczek. The scre...
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The continuous escalation of resistant bacteria against a wide range of antibiotics necessitates discovering novel unconventional sources of antibiotics. B. oleracea L (red cabbage) is health-promoting food with proven anticancer and anti-inflammatory activities. However, it has not been researched adequately for its antimicrobial activity on poten...
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Bladder cancer is the commonest type of malignant tumors as a result of schistosomaisis which is a major healthy problem in many subtropical developing countries. The aim of this study is to comparatively elucidate the underlying biochemical tumor markers in schistosomal bladder cancer versus non-schistosomal bladder cancer when compared to normal...
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Streptococcus bovis (S. bovis) bacteria are associated with colorectal cancer and adenoma. S. bovis is currently named S. gallolyticus. 25 to 80% of patients with S. bovis/gallolyticus bacteremia have concomitant colorectal tumors. Colonic neoplasia may arise years after the presentation of bacteremia or infectious endocarditis of S. bovis/gallolyt...
Patent
Patent no. WO2011098820, UK Application No. 1002295.2. Filing date: 11th February 2010. International Application No. PCT/GB2011/050249. Filing date: 11th February 2011. http://www.wipo.int/patentscope/search/en/detail.jsf?docId=WO2011098820&recNum=203&maxRec=7348&office=&prevFilter=&sortOption=&queryString=%28FP%2Fassay%29+&tab=PCT+Biblio And htt...
Patent
• Malaysian Patent Application No. PI2011001616. • Filing date: 12th April 2011. http://www.rmc.upm.edu.my/upmip/index.php?content=getfaculty&ipid=685&ipdetailid=673&projectlead=151&cluster=3&fac=8
Patent
• Malaysian Patent Application No. PI2011001617. • Filing date: 12th April 2011. http://www.rmc.upm.edu.my/upmip/index.php?content=getfaculty&ipid=683&ipdetailid=671&projectlead=151&cluster=3&fac=8
Article
The aim of this study is to evaluate and quantify the pain relief after minor surgery when certain analgesics are used before surgery. Double blind study was conducted on 300 outpatient surgery patients who were allocated into two groups. Before surgery, 100 mg of acetaminophen was given to one group and 75 mg of diclofenac to the other one. The pa...
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Background: Multiple-drug resistant Acinetobacter have widely spread in the last decades imposing a serious nosocomial source of infection. Nevertheless, little knowledge was gaimed on tracing the development of antibiotic resistance in Acinetobacter species. Objectives: Explore Acinetobacter spp. via antimicrobial susceptibility, plasmid profiles,...
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Colorectal cancer (CRC) has long been associated with bacteremia and/or endocarditis by Streptococcus gallolyticus member bacteria (SGMB) but the direct colonization of SGMB along with its molecular carcinogenic role, if any, has not been investigated. We assessed the colonization of SGMB in CRC patients with history of bacteremia (CRC-w/bac) and w...
Article
Pseudomonas aeruginosa has emerged as a major cause of infection in the last few decades. The objective of this study was to determine the distribution of extended spectrum beta lactamaseproducing P. aeruginosa and the frequency of OXA-2 and OXA-10 genes. 350 and 120 P. aeruginosa samples were isolated from patients in two locations in Iran represe...
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Background: Helicobacter pylori (H. pylori) may be associated with colorectal cancer. However, the underlying mechanisms are still unclear. Objectives: Explore the serostatus of H. pylori cytotoxicity-associated gene A product (CagA) in patients with colorectal carcinoma, and assess the association of H. pylori with colorectal cancer via c-Myc and...
Article
Lipopolysaccharide (LPS) of Brucella abortus is an essential component for developing the subunit vaccine against brucellosis. B. abortus LPS was extracted by n -butanol, purified by ultracentrifugation and detoxified by alkaline treatment. Pyrogenicity and toxicity of B. abortus LPS and detoxified–LPS (D-LPS) were analyzed and compared with LPS of...
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The aim of this study was to determine the changes of short chain fatty acids (SCFAs) in faeces of inflammatory bowel disease (IBD) patients compared to healthy subjects. SCFAs such as pyruvic, lactic, formic, acetic, propionic, isobutyric and butyric acids were analyzed by using high performance liquid chromatography (HPLC). This study showed that...
Article
The aim of this study is to optimize molecular detection and quantification methods of probiotic bacteria in complex microbial communities that have long been difficult for traditional culture-based methods. Traditional and real-time PCR were optimized to detect and quantify Lactobacillus spp. and Bifidobacterium spp. in complex microbial community...
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Background: It was hypothesized that the observed slight immunostimulatory effect of the 23-valent pneumococcal polysaccharide (pneumo-23) vaccine might be due to the presence of low levels of zwitterionic motifs. Therefore, it was hypothesized further that introducing zwitterionic motifs experimentally into polysaccharides of pneumo-23 vaccine mi...