Ahmad Zaheer

Ahmad Zaheer
University of Lahore | UOL · Institute of Molecular Biology and Biotechnology

Doctor of Philosophy

About

64
Publications
219,179
Reads
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617
Citations
Introduction
I am a Biotechnologist, with an aim to perform high quality research, teaching courses and supervising students in their academic assignment and research work.
Additional affiliations
October 2018 - present
The University of Lahore
Position
  • Professor (Assistant)
September 2017 - October 2018
National Institute for Biotechnology and Genetic Engineering
Position
  • PostDoc Position
Description
  • Post-Doctoral Researcher in the project “ Development of next-generation biofertilizer for commercial crops” at National Institute for Biotechnology & Genetic Engineering (NIBGE), Faisalabad, Pakistan.
October 2014 - August 2017
National Institute for Biotechnology and Genetic Engineering
Position
  • Research Associate
Description
  • Research Associate in the PSF-NSLP research project No. 315 “Diversity of symbiotic and free living plant growth promoting rhizobacteria in the root nodules and rhizosphere of chickpea” at NIBGE, Faisalabad
Education
January 2010 - August 2017
February 2007 - April 2009
Quaid-i-Azam University
Field of study
  • Biotechnology
August 2002 - August 2006
PMAS - Arid Agriculture University
Field of study
  • Agriculture

Publications

Publications (64)
Article
Serratia species-affiliated DNA sequences have recently been discovered in the root nodules of two chickpea cultivars; however, little is known about their potential influence on chickpea plant growth. All Serratia-affiliated sequences (1136) could be grouped into two clusters at 98 % DNA similarity. The major cluster, represented by 96 % of sequen...
Article
Full-text available
The main objective of the present study was to isolate phytohormone-producing, phosphate-solubilizing strains of Azospirillum from wheat to be used as inoculants for plant growth promotion. Five Azospirillum strains were isolated from the rhizosphere of field-grown wheat (Triticum aestivum L.), and it was confirmed by BOX-polymerase chain reaction...
Article
Full-text available
In the present study, the relative distribution of endophytic rhizobia in field-collected root nodules of the promiscuous host mung bean was investigated by sequencing of 16S ribosomal RNA (rRNA) and nifH genes, amplified directly from the nodule DNA. Co-dominance of the genera Bradyrhizobium and Ensifer was indicated by 32.05 and 35.84% of the tot...
Article
Full-text available
Objective: Phosphate (P) and zinc (Zn) are essential plant nutrients required for nodulation, nitrogen-fixation, plant growth and yield. Mostly applied P and Zn nutrients in the soil are converted into unavailable form. A small number of soil microbes have the ability to transform unsolvable forms of P and Zn to an available form. P-Zn-solubilizing...
Article
Full-text available
Objective: Maize is an important crop for fodder, food and feed industry. The present study explores the plant-microbe interactions as alternative eco-friendly sustainable strategies to enhance the crop yield. Methodology: Bacterial diversity was studied in the rhizosphere of maize by culture-dependent and culture-independent techniques by soil...
Article
Full-text available
Manganese (Mn) is an essential micronutrient for plant growth that is involved in the structure of photosynthetic proteins and enzymes. Mn deficiency is widespread mainly in dry, calcareous, and sandy soil, which leads to a significant decrease in crop yield. Mn-reducing bacteria promote the solubilization of Mn minerals, thus increasing Mn availab...
Article
Dementia and diabetes are the two major disorders that are linked at both biochemical and molecular levels which is due to the existing similarities between pancreatic beta-cells and neuronal cells at the transcription and translational levels. Both diseases have similar causative genes or factors and dementia is one of the advanced complications i...
Article
Genes differentially regulate transcription processes to maintain biological activities throughout the life of an organism. However, little is known about their fate after organismal death. The Indianmeal moth Plodia interpunctella is a global pest insect that infests stored and manufactured agricultural products. We examined the changes in mRNA le...
Article
Full-text available
Maize is used as staple food in various European countries and it is also used for the production of corn syrups, corn starch and corn ethanol etc. Due to the anthropogenic activities our environment has become polluted. Most of the heavy metals have been eliminated from industrial areas which affect our crop production. The current study was condu...
Article
Full-text available
Heavy metal toxicity affects plant growth, human and animal lives, and microbial activity etc. Heavy metals have badly affected the metabolism and physiological activities of different plants. Heavy metals cannot be destroyed or degraded therefore when their concentration is exceeded they accumulate into the plants and cause toxic effects. Data was...
Article
Full-text available
Waste disposals from industries, commercial, agricultural, medical and automobile are major soil pollutants that contain heavy metals, inorganic and organic compounds etc. In agricultural lands heavy metals accumulated into the soil which effect crop growth, and ultimately food production. Maize genotype Pak-afghoi was used to investigate the effec...
Article
Full-text available
Zinc (Zn) deficiency in major food crops has been considered as an important factor affecting crop production and subsequently the human health. The application of Zn solubilizing bacteria could be a sustainable agronomic approach to increase the soil available Zn. The present field study was conducted at two different sites (Farmers’ fields at Bah...
Preprint
Full-text available
Rice is one of the most important crops for feeding about more than half of the world's population. Yield of rice crop is significantly hampered by various biotic and abiotic factors. Application of cost-effective and environment-friendly bioinoculant is a common practice to combat the yield losses of rice in this era. The rhizosphere and phyllosph...
Conference Paper
Full-text available
Thermophiles are the microorganisms that grow well at high temperatures and are found in specific places such as hot springs, geothermal and volcanic vents. Thermophilic bacteria can produce certain enzymes called thermostable enzymes, which are used for different purposes such as in pharmaceutical, detergent, leather, food, and other industries. S...
Conference Paper
Full-text available
Industrial wastewater contains a high amount of heavy metals that have deleterious impact on aquatic life and enter in human food chain. Among heavy metals, cobalt (Co) is a very toxic element present in wastewater of many industries and affecting the environment. It is present in soluble form that is extremely toxic for any biological function. So...
Article
In Rhizobium-legume symbiosis, the nodule is the most frequently studied compartment, where the endophytic/symbiotic microbiota demands critical investigation for development of specific inocula. We identified the bacterial diversity within root nodules of mung bean from different growing areas of Pakistan using Illumina sequencing of 16S rRNA gene...
Article
Full-text available
Background: Breast cancer is a multifactorial disease with the highest frequency in females. Genetic and environmental factors can cause mutation in several genes like tyrosine kinase, JAK2 gene which may initiate cancer. Molecular analysis of mutations in the JAK2 gene along with determination of environmental, clinical and haematological risk fac...
Article
Full-text available
Objective: Phosphorous is an essential micronutrient of plants and involved in critical biological functions. In nature, phosphorous is mostly present in immobilized inorganic mineral and in the fixed organic form including phytic acid and phosphoesteric compounds. However, the bioavailability of bound phosphorous could be enhanced by the use of p...
Presentation
Full-text available
In present study, the effect of two phosphate-zinc-solubilizing bacterial strains (Pseudomonas stain AZ5 and Bacillus strain AZ17) was evaluated on the yield and quality of winter crop chickpea (Cicer arietinum L.). Zinc and phosphate are essential plant nutrients required for nodulation, nitrogen fixation, plant growth and yield. Mostly applied zi...
Poster
Full-text available
ABSTRACT: Objective: Phosphate (P) and zinc (Zn) are essential plant nutrients required for nodulation, nitrogen-fixation, plant growth and yield. Mostly applied P and Zn nutrients in soil are converted into unavailable form. A small number of soil microbes have ability to transform unsolvable forms of P and Zn to an available form. P-Zn-solubilizi...
Presentation
Full-text available
Different species of the bacterial genus Mesorhizobium are known to induce nitrogen fixing nodules on the roots of chickpea. In the present study, bacterial diversity and distribution of the genus Mesorhizobium in the root nodules and rhizospheric soil of chickpea growing at 5 different localities of Pakistan was investigated by sequencing of PCR-a...
Article
Phosphorus (P) is an essential plant nutrient, but often limited in soils for plant uptake. A major economic constraint in the rice production is excessive use of chemical fertilizers to meet the P requirement. Bioaugmentation of phosphate solubilizing rhizobacteria (PSB) can be used as promising alternative. In the present study 11 mineral PSB wer...
Article
Full-text available
Objective: To study the putative effects of Advanced Oxidation Protein Products (AOPPs) and Advanced Glycation End Products (AGEs) in the development and progression of cardiovascular disease (CVD). Methodology: AGEs, AOPPs, e-NOS, lipid profile, circulating stress and inflammatory biomarkers were evaluated among fifty cardiovascular patients an...
Article
Full-text available
Objective: To study the putative effects of Advanced Oxidation Protein Products (AOPPs) and Advanced Glycation End Products (AGEs) in the development and progression of cardiovascular disease (CVD). Methodology: AGEs, AOPPs, e-NOS, lipid profile, circulating stress and inflammatory biomarkers were evaluated among fifty cardiovascular patients and f...
Article
Full-text available
Objective: To study the putative effects of Advanced Oxidation Protein Products (AOPPs) and Advanced Glycation End Products (AGEs) in the development and progression of cardiovascular disease (CVD). Methodology: AGEs, AOPPs, e-NOS, lipid profile, circulating stress and inflammatory biomarkers were evaluated among fifty cardiovascular patients and f...
Article
Full-text available
Background: Cancer patients when treated with different chemotherapeutic drugs often develop mild to severe sight threatening diseases during or after chemotherapy. The mechanism involved in the pathogenesis of ocular toxicities is poorly understood. Oxidative stress, inflammation and MMPs (angiogenic factor) are involved in the progression of che...
Article
Glycine betaine (GB) is an important organic compound mediating plant responses to environmental stresses. However, despite ample research on this biomolecule, the potential of GB in mitigating the effects of waterlogging in plants has not been established. Therefore, we studied the influence of GB on growth and physiology of tomato plants under wa...
Presentation
Full-text available
In the present study, bacterial diversity and distribution of the genus Mesorhizobium in the root nodules and rhizospheric soil of chickpea growing at 5 different localities of Pakistan was investigated by pyrosequencing of PCR-amplified 16S rRNA and nifH genes. In the nodules, 56.73% of the retrieved 16S rRNA sequences and only a minor fraction 0....
Article
Full-text available
Microbial enzymes play a key role as metabolic catalysts, leading to their diverse applications and use in various industries. The constant search for novel microbial enzymes has led to improvisations in the industrial processes which is the key for profit growth. Actinomycetes form a significant group of microbial populations in soil, plant tissue...
Article
Dry land soils from the Indo-Gangetic plains are inherently poor in organic matter content and phosphorus (P). Amendment of these soils with P-enriched compost, together with P solubilizing rhizobacterial (PSR) inoculation has been suggested to improve plant growth and P uptake. We performed 3 × 2 factorial experiment using compost i.e. control, ra...
Article
Full-text available
Background and objectives: Oral squamous cell carcinoma (OSCC) is regarded as a catastrophe of modern age which affecting millions of people around the globe without any age, gender and race discrimination. Due to low survival, late detection, poor prognosis and accelerated mortality rates, this carcinoma has attained profound attention worldwide....
Article
Full-text available
Background Chronic kidney disease (CKD) is a group of heterogeneous abnormalities affecting the function and structure of the kidney and mostly further proceeds to cardiovascular damage prior to end stage renal disease (ESRD). The oxidative insult and inflammatory mediators have some undefined role in CKD and cardiovascular complications. It is the...
Article
Full-text available
The present study was carried out to evaluate the antimicrobial activity of ethanolic extracts of Azadirachta indica and Psidium guajava against four clinically important bacteria namely Staphylococcus aureus, Salmonella typhi, E. coli and Pseudomonas aeruginosa. The antimicrobial activity of extracts was done with agar well diffusion assay in plat...
Article
Full-text available
Background Ovarian cancer is the 5th most common cause of deaths in the women among gynecological tumors. There are many growing evidences that stress and other behavioral factors may affect cancer progression and patient survival. The purpose of this study is to determine the key role of matrix metalloproteinases (MMPs), and cytokines in the aggre...
Article
Full-text available
Cancer invasion involves a series of fundamental heterogeneous steps, with each step being distinct in its type regarding its dependence on various oncogenic pathways. Over the past few years, researchers have been focusing on targeted therapies to treat malignancies relying not only on a single oncogenic pathway, but on multiple pathways. Scientis...
Article
Full-text available
A cross sectional prospective study was designed to find out the prophetic effect of hyperprolactinemia mediated depression (HMD) in schizophrenics susceptible to thyroid disorders. One hundred newly diagnosed patients (61 males and 29 females) suffering from schizophrenia in the age group of 24-67 years were recruited in the study. Fifty age and s...
Article
Full-text available
Plant growth-promoting rhizobacteria (PGPR) are a group of soil microorganisms that improve plant growth and yield through a number of diverse mechanisms such as phosphate solubilization, nitrogen fixation, production of phytohormones, and repression of soil borne pathogens. Due to high cost of chemical fertilizers and negative environmental effect...
Conference Paper
Full-text available
Background and aims Chickpea is mainly cultivated in nutrient deficient soils in rainfed areas where application of phosphate (P) fertilizer is the main limitation for resource poor farmers. Therefore the present study was conducted to detect P-solubilizing bacteria from field-grown chickpea by DNA-based method, which followed their cultivation for...
Poster
Full-text available
The main objective of the present study was the isolation and identification of the nodule endophyte of mung bean root nodules by morphological characteristics, plant infectivity tests for nodulation and 16S rRNA sequence analysis. Two bacterial isolates obtained from the nodules of mung bean were identified as Ensifer strains on the basis of colon...
Conference Paper
Full-text available
The main objective of the present study was the isolation and identification of the nodule endophyte of mung bean root nodules by morphological characteristics, plant infectivity tests for nodulation and 16S rRNA sequence analysis. Two bacterial isolates obtained from the nodules of mung bean were identified as Ensifer strains on the basis of colon...
Book
Full-text available
Beneficial role of microbes, originating from soil or other sources, has been recognized in many biogeochemical cycles and environmental protection as well as in several processes and products of food, feed and textile industry. In the modern era of scientific advancements, microbial genomes have been utilized not only for improvement of useful pro...
Data
On Oct 1, 2014 this sequence version replaced gi:425025377.
Data
On Sep 30, 2014 this sequence version replaced gi:392841078.
Article
Full-text available
Alkaline proteases are the most important group of extremoenzymes which have a wide array of applications in various industries such as detergent, food, pharmaceutical, leather and silk. For the purpose of maximum alkaline protease production, response surface methodology was performed using Bacillus licheniformis. The sources used were glucose as...
Article
Full-text available
In rainfed farming, livestock is the main source of livelihood as the agriculture crops often confront drought situations and thus may not reach to maturity. Brassica having tape root system has the ability to grow under rainfed conditions. To provide green fodder to livestock during winter fodder scarcity period, an experiment was conducted to com...
Article
Full-text available
Abstract: Resistance (R) genes containing nucleotide binding site (NBS) and leucine rich repeats (LRR) are the most prevalent types of resistance (R) genes in plants. The objective of this study was to isolate, identify and analyze resistance genes from disease (rust) and resistant wheat lines by PCR based strategy. Fifteen degenerate primers were...
Article
Full-text available
The aim of the present study was to isolate and characterize plant growth promoting rhizobacteria from the rhizosphere of field-grown maize. Among the five bacterial isolates obtained in the present study, the isolates M1, M7 and ZN1 showed typical Azospirillum-like cell morphology, motility and high sequence similarity of 16S rDNA to the genus Azo...
Conference Paper
Full-text available
Understanding the causes and consequences of biological diversity is a central goal of biology. Only 1-3% of the soil microbial population is culturable. DNA-based methods have been developed to have an insight into the remaining fraction of soil microflora. Few of these studies have been done aiming at comparison of extreme environment of salinity...
Book
Full-text available
Beneficial role of microbes, originating from soil or other sources, has been recognized in many biogeochemical cycles and environmental protection as well as in several processes and products of food, feed and textile industry. In the modern era of scientific advancements, microbial genomes have been utilized not only for improvement of useful pro...
Article
Full-text available
Plant growth promoting rhizobacteria have gained extensive importance worldwide. These are naturally occurring bacteria that actively colonize the plant roots and improve plant growth. Plants inoculated with some potential PGPR strains exhibit improved biomass production. It has been observed that PGPR exert their growth promoting effects on plants...
Article
Full-text available
The aim of the present study was to isolate phosphate solubilizing bacteria from wheat rhizosphere and investigate their potential for plant growth promotion. Three phosphate solubilizing bacterial strains were isolated by serial dilution method from the rhizosphere of wheat grown under wheat-cotton and wheat-rice crop rotation. 16S rRNA gene seque...
Article
Full-text available
Background: High incidence of breast cancer and its fatal effect has reached an alarming stage across the globe, including the third world countries. Many factors have been reported to be associated with the development of breast cancer but detailed structural and functional information is missing. CA 15-3 is one of the known potential tumor marke...
Thesis
Full-text available
Resistance (R) genes containing nucleotide binding site (NBS) and leucine rich repeats (LRR) are the most prevalent types of resistance (R) genes in plants. The objective of this study was to isolate, identify and analyze resistance genes from disease (rusts) resistant wheat lines by PCR based strategy. Fifteen degenerate primers were designed fr...