Ahmad Aulia Jusuf

• MD. PhD
• University of Indonesia

Aspartame is widely used artificial sweetener. However, chronic exposure to aspartame led to spatial memory impairment and elevated oxidative stress in the brain. Extract of turmeric rhizome (Curcuma longa) (TUR) and extract of bitter melon (Momordica charantia) (BM) is known to have antioxidant activity. The present study was aimed to examine the...

Background Aortic valve replacement with mechanical valves is the standard treatment for aortic valve disease in Indonesia. Its usage is associated with high cost, risk of endocarditis and thromboembolic event, and lifetime consumption of anticoagulants. We performed a novel replacement technique of the aortic valve using an autologous pericardium...

Background: The use of extremely low temperatures in vitrification is known to cause cryoinjury so that it can trigger the activation of the intrinsic apoptotic pathway, which can damage the structural integrity of the pre-antral follicle. Based on that, it is necessary to use an appropriate cryoprotectant to protect the preserved cell. Aims: Th...

Background: Aortic valve replacement with mechanical valves is the standard treatment for aortic valve disease in Indonesia. Its usage is associated with high cost, risk of endocarditis and thromboembolic event, and lifetime consumption of anticoagulants. We perform a novel replacement technique of the aortic valve using an autologous pericardium...

The kidneys are one of the organs affected by microvascular complications due to diabetes mellitus. Hyperglycemia plays an important role in glomerular, mesangial cell, and tubular damage in the kidneys. Metabolic dysregulation, including hyperglycemia, initiates cellular damage in the kidneys. Streptozotocin (STZ) is a chemical compound that is kn...

Background and Objectives: Non-alcoholic Fatty Liver Disease (NAFLD) can occur as a result of micronutrient deficiencies. Hibiscus sabdarifa, a plant used in traditional medicine, contains ingredients that can help prevent this process. This study looked at the potency of Hibiscus sabdariffa Ethanol Extract (HSE) to prevent homocysteine-induced liv...

Topical capsaicin with high concentrations (8%) is effective in the treatment of pain in Post-Herpetic Neuralgia (PHN) and HIV-Associated Distal Sensory Polyneuropathy (HIV-DSP) patients. High concentrations of capsaicin encapsulated in transfersome nanovesicles aim to reduce the irritating ability of capsaicin when using as drugs. Gel preparations...

Background: Longan honey (LH) has the potential as a natural extracellular cryoprotectant to maintain the integrity of intact preantral follicles against the cryoinjury during ovarian vitrification. Objective: This research determined the cryoprotective effects of logan honey on preantral follicles integrity of rat ovary post-vitrification. Mat...

Background Alpha-mangostin (AM) has been shown to have hypoglycemic activity. This study aimed to analyze the effects of AM at a dose of 100 mg/kg and 200 mg/kg to alleviate hyperuricemia and renal dysfunction on high-fat/high-glucose diet and low dose streptozotocin (HF/HG/STZ) injection-induced IR rat model. IR was induced in male Wistar rats by...

BACKGROUND: Many hypotheses regarding the pathophysiology of enterocolitis in aganglionic megacolon or Hirschsprung disease (HSCR) has been proposed. Paneth cells are columnar intestinal epithelial cells that have an important role in maintaining of intestinal homeostasis as a bactericide. Since enterocolitis in HSCR may have association with Panet...

Objective: This study aimed to determine the effects of soybean (Glycine max) administration on ZP2 expression in female mice. Methods: This research used Mus musculus, six-week-old female SWISS strain mice divided into three groups (group without soybean administration and groups with mixed feed with soybeans and pelleted 50:50 and 25:75). Soyb...

Latar Belakang: Penuaan merupakan kondisi yang tidak dapat dihindari yang terjadi sejak lahir dan terus berlangsung sepanjang hidup. Meningkatnya kebutuhan masyarakat dalam menjaga penampilan, bervariasinya kebutuhan dan kondisi tiap individu, serta beragamnya efek samping yang dapat ditimbulkan dari intervensi yang telah ada, menuntut upaya terus...

Background: Burns are defined as tissue damage that occurs as a result of the action of heat. Although many advanced treatments have been made in burn therapy, slow wound healing remains a challenge in burn treatment. Acupuncture can accelerate burn healing through its anti-inflammatory effect, increasing re-epithelialization and angiogenesis. Ob...

Fertility preservation through gamete vitrification has become one of the critical strategies to secure a childbearing potential in patients who are diagnosed with cancer or risks of infertility. Preserving the gametes would prevent the deleterious effects of cancer drugs or radiotherapy exposure on the quality of the gametes. Furthermore, in vitro...

Mitochondria are highly dynamic organelles that can adapt to different environmental stimuli. This study aims to analyze differences in the function and biogenesis of mitochondria and anaerobic glycolysis activity between obese adult rats that were undernourished in childhood (LCD28) and those which were normal (SD28) or already fat (HFD28). Spragu...

Background: Longan honey (LH) has the potential as a natural extracellular cryoprotectant to maintain the integrity of intact preantral follicles against the cryoinjury during ovarian vitrification. Objective: This research determined the cryoprotective effects of logan honey on preantral follicles integrity of rat ovary post-vitrification. Mat...

The structure of follicular tissue affects the ability to maintain the structural integrity of follicles against cryoinjury post-vitrification. Histological analysis was conducted on the structural integrity of each stage of preantral follicles post-vitrification using 7.5% and 15.0% doses of ethylene glycol (EG), and ovarian sections with HE stain...

Ibu hamil yang terpapar bising akan memengaruhi janin yang dikandungnya bahkan memiliki efek negatif terhadap neurogenesis. Suara dari gelombang ritmis (musik) dapat meningkatkan neurogenesis yang berpengaruh langsung terhadap fungsi memori. Penelitian ini bertujuan untuk mengetahui efek positif gelombang ritmis untuk meredam atau menjadi terapi ba...

Oocyte developmental competence is one of the determining factors that influence the outcomes of an IVF cycle regarding the ability of a female gamete to reach maturation, be fertilized, and uphold an embryonic development up until the blastocyst stage. The current approach of assessing the competency of an oocyte is confined to an ambiguous and su...

BACKGROUND Hirschsprung-associated enterocolitis (HAEC) is a life-threatening complication of Hirschsprung’s disease. Studies using animal models on the pathogenesis of HAEC are limited. Thus, this study aimed to establish a rat model of HAEC using topical application of 0.1% benzalkonium chloride (BAC) in the sigmoid colon. METHODS 55 male Sprague...

Metabolic syndrome (MetS) has become a global problem. With the increasing prevalence of MetS worldwide, understanding its pathogenesis and treatment modalities are essential. Animal models should allow an appropriate representation of the clinical manifestations of human conditions. Rats are the most commonly used experimental animals for the stud...

Background: Diabetes mellitus (DM) is a chronic metabolic disease characterized by increased blood sugar levels. The current management of DM to date has a target of controlling blood glucose, but the therapy cannot be separated from long-term drug side effects. Acupuncture can be an option as an adjunct therapy for DM. Objectives: The purpose o...

Background: Gap Junction (GJ) plays a role in supporting the heart electricity. Connexin43 (Cx43) as the main protein constituent of GJ in left cardiac ventricle, will increase in number and slightly redistributed to the lateral sides of cardiomyocytes after aerobic exercise in adulthood. The effects of aerobic exercise that begin at childhood are...

Liver has a high level of Alpha-fetoprotein which is assumed to be important for mammalian development. However, Alpha-fetoprotein knockout studies in mice suggested that despite the absence of Alpha-fetoprotein, mice developed normally; the only abnormality observed was infertility in female mice. There are indications that Afamin, a protein that...

AIM: This study was aimed to determine the antifibrotic activity of Phaleria macrocarpa (PM) extract in liver fibrosis (LF) and its possible mechanism in the rat model. METHODS: Sprague Dawley male rats were injected with 2 mL/kg BW of carbon tetrachloride intraperitoneally twice a week for 2 weeks, followed by 1 mL/kg BW for 6 weeks. Afterward, th...

BACKGROUND: Preeclampsia is a systemic syndrome occurring in 3–5% of pregnancies, caused by disorders of cellular factors resulting in the disruption of trophoblast differentiation and invasion which is important for the placental development and maintaining pregnancy. Cullin-1 is a protein that plays a role in the process of maintaining pregnancy,...

Background: The tissue regeneration process requires high oxygen and energy levels. Cytoglobin (Cygb) is a member of the globin family, which has the ability to bind oxygen, plays a role in dealing with oxidative stress, and carries oxygen into the mitochondria. Energy production for tissue regeneration is associated with mitochondria-especially m...

Background The tissue regeneration process requires high oxygen and energy levels. Cytoglobin (Cygb) is a member of the globin family, which has the ability to bind oxygen, plays a role in dealing with oxidative stress, and carries oxygen into the mitochondria. Energy production for tissue regeneration is associated with mitochondria—especially mit...

Objectives : This study was performed to gain an understanding about perceptions of integrated practical class between histology and anatomical pathology among medical students in Universitas Indonesia, and to evaluate the quality of current histology learning method.Materials and Methods: The students learnt histology by means of histology alone o...

Background Nonalcoholic fatty liver disease (NAFLD) is one of the most widespread chronic liver diseases, caused by the development of insulin resistance. One of the mechanisms involved is a disturbance in insulin signaling by certain toxic substances that interact with one of the proteins responsible for the insulin signaling pathway. Increased h...

Objective: This study aimed to examine the effect of vitrification on apoptosis and survival in human preantral follicles after thawing. Methods: This experimental study was conducted at an acute tertiary care hospital from March 2012 to April 2013. Ovaries were sliced into 5×5×1-mm pieces and divided into the following three groups: preantral f...

The prevalence of obesity in adults is increasing worldwide, which is problematic since obesity is associated with degenerative diseases. Nowadays Indonesia is facing an interesting phenomenon since there are adults who have been obese since childhood and others who conversely were undernourished while young. The biological differences of these two...

Vitrification as a part of the assisted reproductive technology (ART) has become the main concern of reproductive organ preservation. Vitrification is a development of reproduction biotechnology to overcome reproductive problems. Ethylene glycol (EG) as intracellular cryoprotectant has been commonly used in rat ovary vitrification, while skimmed mi...

Vitrification has been widely used to preserve ovarian tissue. However, an efficient method with minimal damaging effect towards the tissue has not been established, yet. Accordingly, the combination method among intra- and extracellular cryoprotectant was conducted in this study. The aim of the research is to evaluate combination of ethylene glyco...

Ethylene Glycol (EG) has been known as cryoprotectant solution for vitrification of biological organs that are widely used. However, using other cryoprotectants that are comparable to EG that are safer to use is considered. The aim of this study is to evaluate the effectiveness of rat (Rattus norvegicus L.) Sprague-Dawley strain ovarian tissue afte...

Preservation of animals and human ovarian tissue by vitrification has been developed to find the efficient methods to overcome the damaging effect from cold shock. The aim of this study is to evaluate the effect of combination of ethylene glycol (EG) as intracellular cryoprotectant and palm dates juice as extracellular cryoprotectant at concentrati...

The role of genes in tissue regeneration process of house gecko (Hemidactylus platyurus) tail is very important to study. However, the PGC-1α gene that plays a role in mitochondrial biogenesis in the tissue regeneration process of house gecko tail has not been studied before. The phylogenetic method can be used to track the determinant species clos...

The house gecko (Hemidactylus platyurus) has evolved the ability to autotomize its tail when threatened. The lost part is then regrown via epimorphic regeneration in a process that requires high energy and oxygen levels. Oxygen demand is therefore likely to outstrip supply and this can result in relative hypoxia in the tissues of the regenerating t...

Background Tissue regeneration is a process that need a high demand of oxygen and energy supply. Cytoglobin (Cygb) is a hexacoordinate globin superfamily that possesses strong oxygen-binding ability. Cygb also has a role in preventing cells from oxidative stress and carrying oxygen into the mitochondria. The production of energy for regeneration is...

Background The tissue regeneration process requires high oxygen and energy levels. Cytoglobin (Cygb) is a member of the globin family, which has the ability to bind oxygen, plays a role in dealing with oxidative stress, and carries oxygen into the mitochondria. Energy production for tissue regeneration is associated with mitochondria—especially mit...

The correlation between cobalamin deficiency and anemia has been studied extensively. However, the effect of cobalamin deficiency on cardiovascular conditions has not been fully understood yet. Our study was conducted to explore the possible cardiovascular function alteration in anemia condition after cobalamin restriction diet. Eighteen adult male...

In the study conducted by Margiana et al., 2015, it has been known that the angle parameters around the point which acts as the meeting point of the lines between the heel and the third fingertip and between finger one and finger five (Q1, Q2, Q3, and Q4) are strongly correlated with Sciatic Functional Index (SFI), Tibial Functional Index (TFI) and...

Auditory stimulation can differently modulate the development of auditory system and hippocampus. Using chicken embryos make it possible for researchers to focus on examining the direct impact of chronic auditoric stress to the development of fetal brain, especially to hippocampus without confounding factors from mother and placenta. We focus to re...

Loud noise exposure during critical gestation periods may increase implantation failure risks and placentation dysregulation or decrease uterine blood flow. Here, this study review published studies on associations between exposure to various prenatal sound types and reproductive outcomes in chicks and speculate the relevance of these studies to pr...

Perlemakan hati merupakan penyakit degeneratif yang dapat berkembang pada masa pertumbuhan. Perlemakan hati dapat terjadi pada dua kondisi yang sangat berbeda jauh secara patofisiologi yaitu kondisi kekurangan kalori dan kelebihan kalori. Penelitian ini bertujuan untuk membandingkan ukuran berat hati pada pemberian diet tinggi kalori, cukup kalori,...

Introduction Myocardial protection is vital to ensure successful open heart surgery. Cardioplegic solution is one method to achieve good myocardial protection. Inevitably, ischemia-reperfusion injury occurs with aortic crossclamping. Histidine-tryptophan-ketoglutarate solution is a frequently used cardioplegia for complex congenital heart surgery....

Objective: This study aimed to show the impact of the ZOL in VCO gel (Ge-ZOL) on the extent of osteoclasts apoptosis.Methods: The study used 27 Sprague-Dawley rats which were divided into three groups: Nine rats in the experimental group were given 40 µg of Ge-ZOL, nine rats in the control group were given VCO emulsion gel without ZOL (Ge-), and ni...

This is preliminary study in order to investigate the effect of dental pulp stem cells (DPSCs) on bone regeneration in an animal model. New Zealand rabbits were used as animal model. The critical defect was created in femoral bone and transplantation of DPSCs applied into bone defect. A colorimetric assay was used to detect ALP level in rabbit’s se...

Introduction: Turbinaria decurren is a brown seaweed species containing fucoidan, a natural sulfated polysaccharide, which has a wide variety of biological activities. Its biological activities have been shown in many studies, including on liver fibrosis model. This study was aimed to characterize fucoidan contents from Turbinaria decurren, taken f...

Background: BDNF is a neurotrophin which plays roles in memory and learning functions. The hippocampus shows BDNF expression to regulate memory function. This study aims to determine the effects of mild aerobic exercises on BDNF expression, the number of pyramidal cells in the CA3 area on hippocampus, memory function and CA3 area ratio to the total...

Introduction: Intermittent hypobaric hypoxia (IHH) is believed to have neuroprotective effect. IHH induces changes in gene expression and intracellular signaling pathways that lead to the emergence of intracellular adaptation through the process of erythropoiesis, angiogenesis, glucose transport and anaerobic glycolysis through HIF-1 alpha gene act...

Introduction: Schematically, embryonic folding and segmental ventrodorsal juxtapositioning theory assert that primitive foregut has different dorsal and ventral parts due to cephalocaudal folding in the fields of trilaminar embryonic plate. However, it is unclear whether all segments of primitive gut endoderm is formed from the ventral and dorsal c...

Transplantation of bone marrow derived stem cells (BMSCs) has been reported inhibits liver fibrosis. Several in vitro studies by co-culturing BMSCs and hepatic stellate cells (HSCs) indirectly or directly in 2D models showed inhibition of HSC as the key player in liver fibrosis. In this study, we investigated direct effect of BMSCs on HSCs by co-cu...

p> Background: Placental hypoxia may lead to oxidative stress, which inflicts damage to capillary protein junction. The aim of this study was to evaluate altered expression of endothelial junction protein of capillaries in hypoxia condition and to observe its correlation with the incidence of intraventricular hemorrhage in premature infants. Metho...

Purpose: To evaluate the effect of povidone iodine and hydrogen peroxide on fracture healing in a rat model. Methods: The middle section of the right femur of 36 male Sprague Dawley rats was osteotomised with a saw. In the control group (n=9), the wound was irrigated with 100 ml 0.9% saline. In the 10% povidone iodine (n=9), 1% povidone iodine (...

Background: Wound heals itself spontaneously as physiological process. However, in some individuals, small wounds such as parenteral injections or body piercings may cause increased expression of collagen synthesis. The condition is known as keloid. Histopathology of keloid demonstrates extensive tissue proliferation that extends beyond the margin...

Expression of beta 2 subunit of Ca²+/calmodulin-dependent protein kinase I (CaMKIβ2) of the rat retina during the developmental period and in the adulthood was studied immunohistochemically. The immunoreactivity of CaMKIβ2 was detected in the earliest development of the primordial retina at embryological day (E) 12. The inner neuroblastic layer fro...

BACKGROUND Prematurity refers to live births before 37 weeks of gestation, wherein the baby is born before the body and its organ systems achieve perfect maturity, and this disorder is still a global problem. The high incidence of prematurity is a problem in developing and also in developed countries. Certain conditions accompanying pregnancies lik...

Several researchers reported that injection of Zoledronate Bisphosphonate (ZOL) could inhibit orthodontic tooth movement by osteoclasts apoptosis, but the administration by injection is painless. Use of ZOL emulsion with olive oil in a gel (Ge-ZOL) is expected to overcome the lack of administration by injection. To proved the effects of exposure of...

Sciatic function index (SFI) has long been used as one of the evaluation methods of walking analysis. SFI is a method in assessing nerve function through footprints in walking analysis. Methods that previously have been developed use one color ink, but this method is difficult to interpret. The interpretation is difficult because of mingled footpri...

Background: Delayed union, nonunion, and mechanical failure is still problems encountered in limb salvage surgery (LSS) using extracorporeal irradiation (ECI). This study aimed to determine whether bone marrow mesenchymal stem cells (MSC) and recombinant human bone morphogenetic protein-2 (rhBMP-2) improve hostgraft union after osteotomy and also...

Background: Scaffold (biomaterial) biocompatibility test should be performed in vitro prior to in vivo stem cell application in animal or clinical trial. These test consists of direct and indirect toxicity test (MTT assay [3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide]). Those tests were used to identify cell morphological changes,...

Objective: The aim of this study was to evaluate the effects of methanol extract of Jatropha multifida leaves on the wound healing process and to investigate the wound healing activity based on reduced numbers of PMN (polymorpho nuclear) leukocytes and increased numbers of fibroblasts. Method: methanol extract of dried leaves of Jatropha multifida...

Objective: The aim of this study was to evaluate the effects of methanol extract of Jatropha multifida leaves on the wound healing process and to investigate the wound healing activity based on reduced numbers of PMN (polymorpho nuclear) leukocytes and increased numbers of fibroblasts. Method: methanol extract of dried leaves of Jatropha multifida...

Aim: to optimize Tenascin-C immunohistochemistry staining protocol for rat cardiac tissue Methods: This is a preliminary experimental study at Histology Lab Faculty of Medicine University of Indonesia from 7th December 2007 to 2nd July 2008. Samples were paraffin sections of rat cardiac tissues, and paraffin sections of brain tissue were used as po...