Agustín Olano

Agustín Olano
Spanish National Research Council | CSIC · Instituto de Investigación en Ciencias de la Alimentación

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235
Publications
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7,981
Citations
Citations since 2017
25 Research Items
2740 Citations
20172018201920202021202220230100200300400500
20172018201920202021202220230100200300400500
20172018201920202021202220230100200300400500
20172018201920202021202220230100200300400500

Publications

Publications (235)
Article
In order to know the catalytic activities of the disaccharidases expressed in the mammalian small intestinal brush-border membrane vesicles (BBMV) high concentrated solutions of sucrose, maltose, isomaltulose, trehalose and the mixture sucrose:lactose were incubated with pig small intestine disaccharidases. The hydrolysis and transglycosylation rea...
Article
We studied the viability of probiotics Lactobacillus acidophilus and Bifidobacterium animalis ssp. lactis and microorganisms of a starter culture for set-style yoghurt, Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus, during manufacture and cold of storage of synbiotic yoghurts containing lactulose and the emerging prebioti...
Article
Enzymatic transgalactosylation, in different concentrated carbohydrate solutions, was investigated using brush border membrane vesicles (BBMV) from the pig small intestine. When lactulose was incubated with BBMV, the hydrolytic activity of the enzyme towards the disaccharide was observed to be very low compared to that towards the lactose, but the...
Article
Artichoke (Cynara scolymus L.) by-products can be used as a good source of pectin. The aim of this work was to compare different pectin extraction methods: power ultrasound (US), enzymes, combination of US and enzymes (US + E), and acids (nitric and sodium citrate). After 6 h, pectin yield was higher when US was applied in combination with Cellucla...
Article
A simple process for purifying lactulose in commercial lactulose syrups was developed. The objective was to establish a selective enzymatic hydrolysis of lactose and epilactose and further removal of monosaccharides by activated charcoal and water/ethanol solutions. Four commercial β-galactosidases from different microorganisms were tested obtainin...
Article
Background: Black garlic (BG)is produced by the heat treatment of fresh garlic (FG)bulbs under controlled conditions of high temperature and high relative humidity for long periods of time without any additional treatment or additives. During this thermal process, changes in the chemical and physicochemical composition of BG are produced in very di...
Article
The fermentation properties of a starter culture during kefir manufacture was studied with the inclusion of one emerging lactulose-derived oligosaccharide prebiotic and with the well-recognised galactooligosaccharide and lactulose prebiotics at different doses (2 and 4%). Microbial growth, glycerol, lactic and citric acids and short-chain fatty aci...
Article
Full-text available
This work highlights the utility of brush border membrane vesicles (BBMV) from the pig small intestine as a reliable model for gathering information about the reaction mechanisms involved in the human digestion of dietary carbohydrates. Concretely, the elucidation of the transgalactosylation mechanism of pig BBMV to synthesize prebiotic galacto-oli...
Article
The feasibility of manufacturing yoghurts with three prebiotics, i.e., fructooligosaccharides (FOS), galactooligosaccharides (GOS) or lactulose added at two concentrations (2 and 4%, w/v) was investigated. Physicochemical and microbial characterisation was conducted on the effect of prebiotics on acidification, viability of Streptococcus thermophil...
Article
Novel artichoke pectic-oligosaccharides (POS) mixtures have been obtained by enzymatic hydrolysis using four commercial enzyme preparations: Glucanex®200G, Pentopan®Mono-BG, Pectinex®Ultra-Olio and Cellulase from Aspergillus niger. Analysis by HPAEC-PAD showed that Cellulase from A. niger produced the greatest amount of POS (310.6 mg g⁻¹ pectin), w...
Article
Full-text available
Colorectal cancer (CRC) is one of the most common neoplasias worldwide, and its incidence is increasing. Consumption of prebiotics is a useful strategy in order to prevent this important disease. These nutraceutical compounds might exert protective biological functions as antitumors. In order to test the chemopreventive effect of GOS-Lu (galacto-ol...
Data
Phyla, families and genera data for each of the animals used in this study.
Data
Raw data on animals weight, caecums weight, number of Peyer’s patches, number of polyps, total tumoral area and short fatty acids concentrations for each of the animals used in this study.
Article
Lactulose-derived oligosaccharides (OsLu) are prebiotic galactooligosaccharides (GOS) beneficial for human health including immunomodulatory properties; however, the molecular mechanism is unclear. OsLu produced by enzymatic synthesis can be purified with Saccharomyces cerevisiae (OsLu-Sc). We show that this purification introduces yeast-derived pr...
Article
This study compares two different procedures for purification of lactulose-derived galactooligosaccharides (GOS) using fresh Saccharomyces cerevisiae yeast or activated charcoal with water or ethanol/water solutions for selective removal of monosaccharides. Yeast fermentation allowed the complete removal of monosaccharides without any losses of di-...
Article
The aim of this study was to optimise pectin extraction from artichoke by-products with Celluclast®1.5L using an experimental design analysed by response-surface methodology (RSM). The variables optimised were artichoke by-product powder concentration (2–7%, X1), enzyme dose (2.2–13.3 U g⁻¹, X2) and extraction time (6–24 h, X3). The variables studi...
Article
Evaluation of Maillard reaction (MR) in commercial prebiotic-supplemented and not supplemented infant formulas (IFs) was carried out through determination of furosine (2-furoylmethyl-ε-lysine) and hydroxymethylfurfural (HMF). Furosine was present in all studied IFs, ranging from 94 to 1226 and 315 to 965 mg 100 g⁻¹ of protein, in samples without pr...
Article
Full-text available
This study reports the formation of 2-furoylmethyl amino acids (2-FM-AA) as indicators of Maillard reaction (MR) in black garlic elaboration, followed by the determination of furosine by ion-pair RP-HPLC-UV. The method was assessed for accuracy, repeatability and detection and quantitation limits indicating its adequacy. Traditional procedure of bl...
Article
Background: One of the most promising uses of whey permeate (WP) is the synthesis of prebiotic oligosaccharides. Herein, commercial WP was submitted to chemical isomerisation catalysed by sodium borate at an alkaline pH and subsequent purification using anion-exchange resins to remove boron. Afterwards, purified mixtures were used to synthesise pr...
Article
The increasing knowledge on the complexity of the gut microbiota and its interaction with the host is challenging the concept and definition of prebiotics. This review emphasizes the recent advances in the enzymatic production of prebiotic oligosaccharides, the use of renewable and/or alternative sources, i.e. seaweeds and marine microalgae, and th...
Article
Abstract Since breastfeeding is not always possible, infant formulas (IFs) are supplemented with prebiotic oligosaccharides, such as galactooligosaccharides (GOS) and/or fructooligosaccharides (FOS) to exert similar effects to those of the breast milk. Nowadays, a great number of infant formulas enriched with prebiotics are disposal in the market,...
Article
Full-text available
b-galactosidase from Aspergillus oryzae offers a high yield for the synthesis of oligosaccharides derived from lactulose (OsLu) by transgalactosylation. Oligosaccharides with degree of polymerization (DP) ≥ 3 have shown to possess higher in vitro bifidogenic effect than di- and tetrasaccharides. Thus, in this work, an optimization of reaction condi...
Article
An evaluation of the formation of prebiotic carbohydrates during lactose hydrolysis has been carried out in industrially elaborated dairy preparations designed for elderly people. Due to the hydrolysis of lactose, high levels of galactose and glucose were found together with galactooligosaccharides (GOS), mainly allolactose, 6-galactobiose and 6'-g...
Article
Full-text available
This article reports the efficient enzymatic synthesis of a homologous series of isomaltulose-derived oligosaccharides with degrees of polymerization ranging from 3 to 9 through the transglucosylation reaction using a dextransucrase from Leuconostoc mesenteroides B-512F. The total oligosaccharide yield obtained under optimal conditions was of 41-42...
Article
The increasing interest for prebiotic carbohydrates as functional food ingredients has promoted the synthesis of galactooligosaccharides and new lactose derivatives. This review provides a comprehensive overview on the chromatographic analysis, structural characterization, and bioactivity studies of lactose‐derived oligosaccharides. The most comm...
Article
In this paper, a study on the usefulness of the determination of vitamin C together with indicators of the initial steps of Maillard reaction (2-furoylmethyl amino acids, 2-FM-AA) during the convective drying of strawberries has been carried out for the first time, paying special attention to the kinetics of degradation and formation, respectively,...
Article
Intestinal microbiota modulation is becoming an interesting approach to manage inflammatory bowel disease, and can be achieved by the administration of prebiotics. Previous studies showed the intestinal anti-inflammatory effects of the prebiotic lactulose. The aim of the present study was to test the preventative effects of oligosaccharides derived...
Article
Full-text available
Prebiotic oligosaccharides are increasingly demanded within the Food Science domain because of the interesting healthy properties that these compounds may induce to the organism, thanks to their beneficial intestinal microbiota growth promotion ability. In this regard, the development of new efficient, convenient and affordable methods to obtain th...
Article
In an attempt to offer a broad overview on lactose applications as a source of bioactive carbohydrates, in this chapter, the authors have collected the most recent investigations on methods to produce these compounds, which have a positive effect on gut microbiota. Chemical and biological productions of tagatose, lactulose, lactosucrose, lactulosuc...
Article
Low molecular weight chitosan (LMWC) and chitooligosaccharide (COS) derivatives were obtained by the introduction of lactobionic acid (LA) through amide formation, obtaining different complexes COS-LA and LMWC-LA (1–5), with a degree of substitution (DS) between 3 and 16%. The synthesis of these derivatives was monitored by Scanning Electron Micros...
Article
There is increasing interest in identifying novel dietary non-digestible carbohydrates capable of modulating the composition and/or metabolic activities of the gut microbiota. In this work, we have assessed the differential modulatory influence of novel galacto-oligosaccharides derived from lactulose (GOS-Lu) in comparison with commercial galacto-o...
Article
Full-text available
ALIBIRD, a test substance composed of oligosaccharides derived from lactulose, a hydrolysate of a whey protein concentrate, and a supercritical extract of rosemary (1:0.5:0.05), was prepared in the laboratory and evaluated for its safety as a multifunctional food additive. In oral toxicity studies (acute and 28 days repeated dose) using Wistar rats...
Article
Background: Lactose from cheese whey permeate (WP) was efficiently isomerised to lactulose using egg shell, a food-grade catalyst, and the subsequent transgalactosylation reaction of this mixture with β-galactosidase from Bacillus circulans gave rise to a wide array of prebiotic carbohydrates derived from lactose and lactulose. Results: Lactulos...
Article
In this work a study of mono-, di- and galactooligosaccharide (GOS) content as well as the determination of thermal indicators of different commercial lactose-free UHT (LF-UHT) milks and dairy drinks has been carried out. Moreover, GOS formation during hydrolysis of lactose in a commercial UHT milk using Lactozym (R) pure 6500 was also studied. Pre...
Article
The effect of drying methods on chitosan reactivity towards Maillard reaction during storage of dried chitosan–lactose systems was investigated. Two different structural forms of chitosan, scaffold and microspheres, were prepared. Then they were dried by lyophilization or using supercritical fluid technology (SF-CO2) and stored with lactose under c...
Article
This work describes an efficient enzymatic synthesis and NMR structural characterization of the trisaccharide β-d-galactopyranosyl-(1→4)-β-d-fructofuranosyl-(2→1)-α-d-glucopyranoside, also termed as lactulosucrose. This oligosaccharide was formed by the Leuconostoc mesenteroides B-512F dextransucrase-catalyzed transfer of the glucosyl residue from...
Article
Production of prebiotic carbohydrates at competitive prices is a challenge nowadays since the well-established production processes involve many purification steps which are labour intensive and require important amounts of reagents and products thus increasing prebiotic's price. Several processes have been studied in our laboratory involving the u...
Article
Fermentation properties of oligosaccharides derived from lactulose (OsLu) and lactose (GOS) have been assessed in pH-controlled anaerobic batch cultures using lactulose and Vivinal-GOS as reference carbohydrates. Changes in gut bacterial populations and their metabolic activities were monitored over 24 h by fluorescent in situ hybridization (FISH)...
Article
The production of new bioactive oligosaccharides is currently garnering much attention for their potential use as functional ingredients. This work addresses the enzymatic synthesis and NMR structural characterization of 2-α-D-glucopyranosyl-lactose derived from sucrose:lactose and sucrose:cheese whey permeate mixtures by using a Leuconostoc mesent...
Article
The influence of cations, Na + and K + on the oligosaccharides produced in the process of transglycosylation during the hydrolysis of lactose using β-galactosidase from Kluyveromyces (K.) lactis has been investigated. The enzyme exhibited higher hydrolytic activity in the presence of K + than in that of Na + regardless of the anion present (acetate...
Conference Paper
Full-text available
Study for Galacto-oligosaccharides Production using Supercritical Fluid Extraction Technology F. Montañs1, T.Fornari2, A. Olano2 and E. Ibáñez2 1Integrated Bioactive Technologies, Industrial Research Limited. 69 Gracefield Road, 5040 Lower Hutt, New Zealand 2Instituto de Investigación en Ciencias de la Alimentación, C/ Nicolás Cabrera 9, Camp...
Article
The influence of reaction conditions for oligosaccharide synthesis from stachyose using a commercial enzymatic preparation from Aspergillus aculeatus (Pectinex Ultra SP-L) was studied. Oligosaccharides were analyzed by gas chromatography with flame ionization detection (GC-FID) and matrix-assisted laser desorption/ionization-time-of-flight-mass spe...
Article
We have studied the effect of pH (6.5, 7.5), temperature (40, 50 °C), time (2–24 h) as well as lactulose (250, 450 g/L) and enzyme (3, 6, 9 U/mL) concentration on the formation of 6′-galactosyl-lactulose (β-D-Galp(1 → 6)-β-D-Galp(1 → 4)-D-Fru) and 1-galactosyl-lactulose (β-D-Galp(1 → 4)-β-D-Frup(1 → 1)-D-Gal) formed during the transgalactosylation...
Article
The effect of structure on the fermentative properties of potential prebiotic trisaccharides derived from lactulose like 6'-galactosyl-lactulose (β-d-galactopyranosyl-(1→6)-β-d-galactopyranosyl-(1→4)-β-d-fructopyranose), 4'-galactosyl-lactulose (β-d-galactopyranosyl-(1→4)-β-d-galactopyranosyl-(1→4)-β-d-fructopyranose), and 1-galactosyl-lactulose (β...
Article
Nowadays, the application of green chemistry principles in the production of new polymeric materials is receiving an increasing attention. In the present work, we have investigated the impregnation of chitosan with lactulose using supercritical fluids under various operating conditions, in order to improve the solubility of this natural polymer at...
Article
Full-text available
This work explores the potential of high-intensity ultrasound to produce fine-dispersion, long-time-stable, oil-in-water emulsions prepared with native and glycated bovine sodium caseinate (SC). Regardless the ultrasound amplitude and time assayed, the sonicated emulsions of native SC at 0.5 mg/mL had much higher emulsifying activity indexes compar...
Article
Prebiotic carbohydrates are one of the most interesting and challenging developments in nutrition in recent years because they can be easily included in the diet. There is a strong evidence of the beneficial effect of prebiotics in humans, namely the ability to stimulate the growth of bifidobacteria and lactobacilli, the increase of the short chain...
Article
Food-grade complex oligosaccharide mixtures manufactured commercially via transglycosylase reactions using disaccharides, such as sucrose or lactose as the raw material, are, in general, not pure products. Reducing oligosaccharides may be isomerized using borates and aluminates as complexating agents to obtain prebiotic oligosaccharides. Complexati...
Article
Tesis doctoral inédita. Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Química Física Aplicada. Fecha de lectura: 06-11-2009 Bibliogr.: p.153-164
Article
The effect of pyridoxamine on the Maillard reaction during the formation of conjugates of beta-lactoglobulin with galactose and tagatose under controlled conditions (pH 7, 0.44 aw, 40 and 50 degrees C, for 6 days) has been studied, for the first time, by means of the changes in reducing carbohydrates, formation of Amadori or Heyns compounds, and ag...
Article
Four different fractionation techniques for obtaining galactooligosaccharides (GOS) free from digestible carbohydrates were compared. Diafiltration did not show any selectivity among mono-, di- and oligosaccharides, whereas yeast treatment to remove monosaccharides allowed a high recovery of GOS and disaccharides. Activated charcoal treatment showe...
Article
Lactulose is a synthetic ketose disaccharide that can be obtained from lactose by different methods of synthesis. Chemical methods are based on the isomerization of lactose in the presence of basic catalysts and enzymatic methods using lactose as a galactose donor and fructose as an acceptor. The prebiotic properties of lactulose have been known fo...
Article
The commercial enzyme preparation Pectinex Ultra SP-L containing fructosyltransferase activity was used to hydrolyze stachyose. During this reaction, besides the formation of mono-, di-, and trisaccharides (DP(3)), the presence of one pentasaccharide (DP(5)) and a new oligosaccharide (DP(6)) has been detected by gas chromatography. DP(5) and DP(6)...
Article
Full-text available
Prebiotic carbohydrates are defined as non-digestible carbohydrates that beneficially affect the host by selectively stimulating the growth and/or activity of a limited number of bacteria in the colon.The present study is focused on two prebiotic ketosugars: tagatose and lactulose, which are currently obtained by alkaline isomerization of their res...
Article
In the present work, supercritical technology based on carbon dioxide has been used as an alternative to fractionate galactooligosaccharides (GOS) in a dairy-based prebiotic ingredient. The main challenge of the fractionation of the commercial mixture is the combination of GOS with monosaccharides and disaccharides. Considering that the beneficial...
Article
BACKGROUND: Processing of vegetables in the food industry usually includes dehydration as a preservation process. Industrial convective air drying of carrot can involve steam blanching of the raw product after peeling and cutting, and different stages of dehydration (first space, second space and final drying). Although the shelf-life of carrot is...
Article
Galactooligosaccharides (GOS) obtained during the enzymatic hydrolysis of lactose contain large amounts of glucose, galactose, and unreacted lactose, which do not have prebiotic properties and increase the calorific value of the product. In this work, the isomerization of the GOS mixture by the action of sodium aluminate has been studied. During th...
Article
A simple high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) method was validated to determine the galactooligosaccharide (GOS) content of 14 commercially available fermented milks (yogurts, yogurts containing bifidobacteria, and ready-to-drink yogurts containing Lactobacillus casei) marketed in Spain. The...
Article
A mass spectrometric study has been carried out to elucidate the structures of glycated peptides obtained after in vitro gastrointestinal digestion of bovine beta-lactoglobulin (beta-LG) glycated with prebiotic galacto-oligosaccharides (GOS). The digests of both native and glycated beta-LG were analyzed by MALDI-MS, LC-ESI-MS, and LC-ESI-MS/MS. MAL...
Article
To investigate the influence of the type of carbonyl group of the sugar on the structural changes of proteins during glycation, an exhaustive structural characterization of glycated beta-lactoglobulin with galactose (aldose) and tagatose (ketose) has been carried out. Conjugates were prepared via Maillard reaction at 40 and 50 degrees C, pH 7, and...
Article
Nowadays consumers hold high standards for the foods they consume and they also demand for products that can provide added healthful benefits; two of these products with an observed prebiotic activity, tagatose and lactulose, are the core of our study. These two carbohydrates are ketoses and have important biological activities, such as the stimula...
Article
The beta-galactosidase activity of the commercial enzymatic preparation Pectinex Ultra SP-L derived from Aspergillus aculeatus has been used to hydrolyze and transgalactosylate the prebiotic carbohydrate lactulose. During this reaction, new oligosaccharides derived from lactulose have been detected by high-performance anion-exchange chromatography...
Article
BACKGROUND: Galacto-oligosaccharides (GOS) are synthesised from lactose by transglycosylation using β-galactosidase (EC 3.2.1.23) and are recognised as prebiotics. The commercial enzyme preparation Pectinex Ultra SP-L produced by Aspergillus aculeatus possesses β-galactosidase activity; however, because its use has been directed towards the formati...
Article
A study on optimisation of the conditions for galactooligosaccharide (GOS) formation during lactose hydrolysis, produced by Lactozym 3000 L HP G, was carried out. The synthesis was performed during times up to 300 min at 40, 50 and 60 °C, pH 5.5, 6.5 and 7.5, lactose concentration 150, 250 and 350 mg/mL and enzyme concentration 3, 6 and 9 U/mL. The...
Article
The enzymatic transgalactosylation during lactulose hydrolysis was studied using the beta-galactosidase from Kluyveromyces lactis and an initial lactulose concentration of 250 g/L. During hydrolysis of lactulose, the formation of two novel trisaccharides was followed by high-performance anion-exchange chromatography with pulsed amperometric detecti...
Article
Galactooligosaccharides (GOS) are well-known prebiotic ingredients which can form the basis of new functional dairy products. In this work, the production and characterization of glycated beta-lactoglobulin (beta-LG) with prebiotic GOS through the Maillard reaction under controlled conditions ( a(w) = 0.44, 40 degrees C for 23 days) have been studi...
Article
Solubility of six different carbohydrates in methanol, ethanol, 1-propanol, and 2-propanol were measured at 22, 30, and 40°C. Ketose sugars (fructose, tagatose, and lactulose) show higher solubilities than aldoses (glucose, galactose, and lactose). The binary solid–liquid equilibrium data obtained was satisfactory represented by using the A-UNIFAC...
Article
The viability of the purification of lactulose from a mixture with lactose [70:30 (w/w)] using pressurized liquid extraction (PLE) at 1500 psi for 30 min was studied. Different temperatures (from 40 to 130 degrees C) and proportions of ethanol:water (70:30, 80:20, 90:10, 95:5, and 100:0) as the extraction solvent were assayed. Lactose and lactulose...
Article
The combined effect of gluten glycation and proteolysis on the release of compounds exhibiting in vitro angiotensin-converting enzyme (ACE) inhibitory, antioxidant and antibacterial activities was investigated. Model systems consisting of wheat gluten and glucose were heated at 120 °C for 45 min, 150 °C for 30 min and 220 °C for 30 min to produce v...
Article
A simple, economical and non-toxic method is described for the solubilization of undenatured β-lactoglobulin (β-lg) from complexes with chitosan. The effect of pH (8–10), ionic strength (0.08–0.3 m) and volume ratio between sodium acetate solutions and whey on the dissociation of β-lg–chitosan complexes was evaluated. Following a single extraction...
Article
In the present work, the viability of the selective fractionation of carbohydrate mixtures (lactose–lactulose) using supercritical CO2 has been evaluated. As a first step towards the optimization of the fractionation process, the composition of the co-solvent used in the supercritical phase was studied by considering different ethanol:water mixture...
Article
Lysinoalanine (LAL) is an unwanted byproduct, which is formed during the processing of protein and protein-containing foods and feeds. A GC method for the quantitative analysis of LAL under conventional chromatographic conditions has been developed. The method was applied to the analysis of pure standard substances, boiled eggs, commercial caseinat...
Article
Soy protein isolate (SPI) was glycated with fructose or fructooligosaccharides (FOS) through the Maillard reaction in powder and liquid systems. A reduction in primary free amino groups of SPI up to 85% and 96% was observed in the powder and liquid system, respectively. Following heating at 95 °C for 1 h under liquid conditions, the electrophoretic...