Agustín Mangiarotti

Agustín Mangiarotti
Max Planck Institute of Colloids and Interfaces | MPIKG · Department of Theory and Bio-Systems

PhD

About

22
Publications
2,824
Reads
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272
Citations
Introduction
My research interests are focused on membrane biophysics and the effects of macromolecular crowding and liquid droplets on membrane properties.
Additional affiliations
April 2019 - March 2021
Instituto de Investigación Médica Mercedes y Martín Ferreyra
Position
  • PostDoc Position
Description
  • Water dynamics in crowded biomimetic systems - Postdoc under the supervision of Dr. Luis Bagatolli
April 2018 - March 2019
Centro de Investigaciones en Química Biológica de Córdoba
Position
  • PostDoc Position
Description
  • Comparison of the influence of hopanoids and sterols in membrane properties - Postdoc under the supervision of Dr. Natalia Wilke
July 2017 - September 2017
Indiana University-Purdue University Indianapolis
Position
  • Fellow
Description
  • Determination of Lipid Dynamics in Phase Segregated Biomembranes Using Fluorescence Correlation Spectroscopy (FCS) - under supervision of Dr. Christoph A. Naumann
Education
August 2013 - December 2017
National University of Cordoba, Argentina
Field of study
  • Membrane Biophysics
January 2008 - December 2012

Publications

Publications (22)
Article
Full-text available
Endomembrane damage represents a form of stress that is detrimental for eukaryotic cells1,2. To cope with this threat, cells possess mechanisms that repair the damage and restore cellular homeostasis3–7. Endomembrane damage also results in organelle instability and the mechanisms by which cells stabilize damaged endomembranes to enable membrane rep...
Article
E. coli biofilms consist of bacteria embedded in a self‐produced matrix mainly made of protein fibers and polysaccharides. The curli amyloid fibers found in the biofilm matrix are promising versatile building blocks to design sustainable bio‐sourced materials. To exploit this potential, it is crucial to understand i) how environmental cues during b...
Article
Full-text available
Membrane wetting by biomolecular condensates recently emerged as a key phenomenon in cell biology, playing an important role in a diverse range of processes across different organisms. However, an understanding of the molecular mechanisms behind condensate formation and interaction with lipid membranes is still missing. To study this, we exploited...
Article
Full-text available
Cells compartmentalize parts of their interiors into liquid-like condensates, which can be reconstituted in vitro. Although these condensates interact with membrane-bound organelles, their potential for membrane remodeling and the underlying mechanisms of such interactions are not well-understood. Here, we demonstrate that interactions between prot...
Preprint
Full-text available
Membrane wetting by biomolecular condensates recently emerged as a critical phenomenon in cell biology, involved in a great diversity of processes across different organisms. However, understanding the molecular mechanism behind this process is still missing. Exploiting ACDAN and LAURDAN properties as nano-environmental sensors in combination with...
Preprint
Full-text available
Microbial biofilms are multicellular communities where bacteria produce an extracellular matrix mainly consisting of proteins and polysaccharides. These biofilms not only confer resistance against external stresses (e.g. antibiotics), but their physical and chemical properties can also adapt to environmental conditions (e.g. temperature, humidity,...
Preprint
Cells compartmentalize their components in liquid-like condensates, which can be reconstituted in vitro. Although these condensates interact with membrane-bound organelles, the potential of membrane remodeling and the underlying mechanisms are not well understood. Here, we demonstrate that interactions between protein condensates and membranes can...
Article
Using LAURDAN fluorescence we observed that water dynamics measured at the interface of DOPC bilayers can be differentially regulated by the presence of crowded suspensions of different proteins (HSA, IgG, Gelatin) and PEG, under conditions where the polymers are not in direct molecular contact with the lipid interface. Specifically, we found that...
Article
Important concepts from colloidal physical chemistry such as coacervation, phase transitions, emergent properties and ionic association, are currently emerging in the lexicon of cellular biology, prompted mostly by recent experimental observations of liquid phase coexistence in the cell cytosol. Nevertheless, from an historical point of view, the a...
Article
Full-text available
The membrane translocation efficiency of cell penetrating peptides (CPPs) has been largely studied, and poly-arginines have been highlighted as particularly active CPPs, especially upon negatively charged membranes. Here we inquire about the influence of membrane mechanical properties in poly-arginine adsorption, penetration and translocation, as w...
Article
In recent years, hopanoids, a group of pentacyclic compounds found in bacterial membranes, are in the spotlight since it was proposed that they induce order in lipid membranes in a similar way cholesterol do in eukaryotes, despite their structural differences. We studied here whether diplopterol (an abundant hopanoid) promoted similar effects on mo...
Article
Hopanoids are pentacyclic molecules present in membranes from some bacteria, recently proposed as sterol-surrogates in these organisms. Diplopterol is an abundant hopanoid that, similar to sterols, do not self-aggregate in lamellar structures when pure, but forms monolayers at the air-water interface. Here we analyze the interfacial behavior of pur...
Article
In model lipid membranes with phase coexistence, domain sizes distribute in a very wide range, from the nanometer (reported in vesicles and supported films) to the micrometer (observed in many model membranes). Domain growth by coalescence and Ostwald ripening is slow (minutes to hours), the domain size being correlated with the size of the capture...
Article
For decades, it has been assumed that electrostatic long-range (micron distances) repulsions in lipid bilayers are negligible due to screening from the aqueous milieu. This concept, mostly derived from theoretical calculations, is broadly accepted in the biophysical community. Here we present experimental evidence showing that domain-domain electro...
Article
We compared the thermodynamic behavior of supported and free-standing films of phospholipids with different chain length and showed that the change in free energy for the phase transition to a denser state was greater in free-standing than in supported membranes, with the differences being independent of the chain length. The presence of the suppor...
Article
In membranes with phase coexistence, line tension appears as an important parameter for the determination of the amount of domains, as well as their size and their shape, thus defining the membrane texture. Different molecules have been proposed as “linactants” (i.e. molecules that reduce the line tension, thereby modulating the membrane texture)....
Article
For the biophysical study of membranes, a variety of model systems have been used to measure the different parameters and to extract general principles concerning processes that may occur in cellular membranes. However, there are very few reports in which the results obtained with the different models have been compared. In this investigation, we q...
Article
The surface dilational modulus -or compressibility modulus- has been previously studied for monolayers composed of pure materials, where a jump in this modulus was related with the onset of percolation as a result of the establishment of a connected structure at the molecular level. In this work, we focused on monolayers composed of two components...

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