Agustín Mangiarotti

Agustín Mangiarotti
Max Planck Institute of Colloids and Interfaces | MPIKG · Department of Theory and Bio-Systems

PhD

About

16
Publications
1,703
Reads
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221
Citations
Citations since 2016
11 Research Items
199 Citations
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Introduction
My research interests are focused on membrane biophysics and the effects of macromolecular crowding and liquid droplets on membrane properties.
Additional affiliations
April 2019 - March 2021
Instituto de Investigación Médica Mercedes y Martín Ferreyra
Position
  • PostDoc Position
Description
  • Water dynamics in crowded biomimetic systems - Postdoc under the supervision of Dr. Luis Bagatolli
April 2018 - March 2019
Centro de Investigaciones en Química Biológica de Córdoba
Position
  • PostDoc Position
Description
  • Comparison of the influence of hopanoids and sterols in membrane properties - Postdoc under the supervision of Dr. Natalia Wilke
July 2017 - September 2017
Indiana University-Purdue University Indianapolis
Position
  • Fellow
Description
  • Determination of Lipid Dynamics in Phase Segregated Biomembranes Using Fluorescence Correlation Spectroscopy (FCS) - under supervision of Dr. Christoph A. Naumann
Education
August 2013 - December 2017
National University of Cordoba, Argentina
Field of study
  • Membrane Biophysics
January 2008 - December 2012

Publications

Publications (16)
Preprint
Full-text available
Microbial biofilms are multicellular communities where bacteria produce an extracellular matrix mainly consisting of proteins and polysaccharides. These biofilms not only confer resistance against external stresses (e.g. antibiotics), but their physical and chemical properties can also adapt to environmental conditions (e.g. temperature, humidity,...
Preprint
Cells compartmentalize their components in liquid-like condensates, which can be reconstituted in vitro. Although these condensates interact with membrane-bound organelles, the potential of membrane remodeling and the underlying mechanisms are not well understood. Here, we demonstrate that interactions between protein condensates and membranes can...
Article
Using LAURDAN fluorescence we observed that water dynamics measured at the interface of DOPC bilayers can be differentially regulated by the presence of crowded suspensions of different proteins (HSA, IgG, Gelatin) and PEG, under conditions where the polymers are not in direct molecular contact with the lipid interface. Specifically, we found that...
Article
Important concepts from colloidal physical chemistry such as coacervation, phase transitions, emergent properties and ionic association, are currently emerging in the lexicon of cellular biology, prompted mostly by recent experimental observations of liquid phase coexistence in the cell cytosol. Nevertheless, from an historical point of view, the a...
Article
Full-text available
The membrane translocation efficiency of cell penetrating peptides (CPPs) has been largely studied, and poly-arginines have been highlighted as particularly active CPPs, especially upon negatively charged membranes. Here we inquire about the influence of membrane mechanical properties in poly-arginine adsorption, penetration and translocation, as w...
Article
In recent years, hopanoids, a group of pentacyclic compounds found in bacterial membranes, are in the spotlight since it was proposed that they induce order in lipid membranes in a similar way cholesterol do in eukaryotes, despite their structural differences. We studied here whether diplopterol (an abundant hopanoid) promoted similar effects on mo...
Article
Hopanoids are pentacyclic molecules present in membranes from some bacteria, recently proposed as sterol-surrogates in these organisms. Diplopterol is an abundant hopanoid that, similar to sterols, do not self-aggregate in lamellar structures when pure, but forms monolayers at the air-water interface. Here we analyze the interfacial behavior of pur...
Article
In model lipid membranes with phase coexistence, domain sizes distribute in a very wide range, from the nanometer (reported in vesicles and supported films) to the micrometer (observed in many model membranes). Domain growth by coalescence and Ostwald ripening is slow (minutes to hours), the domain size being correlated with the size of the capture...
Article
For decades, it has been assumed that electrostatic long-range (micron distances) repulsions in lipid bilayers are negligible due to screening from the aqueous milieu. This concept, mostly derived from theoretical calculations, is broadly accepted in the biophysical community. Here we present experimental evidence showing that domain-domain electro...
Article
We compared the thermodynamic behavior of supported and free-standing films of phospholipids with different chain length and showed that the change in free energy for the phase transition to a denser state was greater in free-standing than in supported membranes, with the differences being independent of the chain length. The presence of the suppor...
Article
In membranes with phase coexistence, line tension appears as an important parameter for the determination of the amount of domains, as well as their size and their shape, thus defining the membrane texture. Different molecules have been proposed as “linactants” (i.e. molecules that reduce the line tension, thereby modulating the membrane texture)....
Article
For the biophysical study of membranes, a variety of model systems have been used to measure the different parameters and to extract general principles concerning processes that may occur in cellular membranes. However, there are very few reports in which the results obtained with the different models have been compared. In this investigation, we q...
Article
The surface dilational modulus -or compressibility modulus- has been previously studied for monolayers composed of pure materials, where a jump in this modulus was related with the onset of percolation as a result of the establishment of a connected structure at the molecular level. In this work, we focused on monolayers composed of two components...

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Projects

Project (1)
Project
Biomembranes are very active organelles in intermolecular communication and cell motion, and therefore, they present a very specific selective permeability and mechanical properties. In the membrane, several biochemical reactions occur, being the starting point of a variety of signaling cascades. However, as a result of the complexity and temporal variation of the membrane structure and properties, their functioning and regulation is still not completely known. Our objective is to describe the energetics of membrane deformations in the plane and out of the plane of the membrane, and how this is affected by the membrane composition, electrostatics and interaction with the environment.