• Home
  • Agnieszka Wolińska
Agnieszka Wolińska

Agnieszka Wolińska
John Paul II Catholic University of Lublin, Institute of Biological Sciences · Biology and Biotechnology of Microorganisms

dr hab prof KUL

About

81
Publications
41,795
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,352
Citations

Publications

Publications (81)
Article
Full-text available
The main goal of the study was to determine changes in the bacterial structure in bottom sediments occurring over the seasons of the year and to estimate microbial metabolic activity. Bottom sediments were collected four times in the year (spring, summer, autumn, and winter) from 10 different measurement points in Cardinal Pond (Ślesin, NW Poland)....
Article
Full-text available
The choice of appropriate agricultural practices has a significant impact on soil quality, crop productivity, or soil microbial community. Ten representative soil samples were collected in Wierzchucin Królewski (Kujawsko-Pomorskie Province, NW Poland), an agricultural area belonging to the Potulicka Foundation Group. The cropping systems included a...
Article
Full-text available
Although fungi are regarded as very important components of soils, the knowledge of their community in agricultural (monocultural) soils is still limited. This indicates that soil fungal communities are investigated less intensively than bacteria. Therefore, the main goal of this paper was to evaluate the fungal mycobiome structure in monoculture s...
Article
Full-text available
As an adaptation to unfavorable conditions, microorganisms may represent different phenotypes. Azolla filiculoides L. is a hyperaccumulator of pollutants, but the functions of its microbiome have not been well recognized to date. We aimed to reveal the potential of the microbiome for degradation of organic compounds, as well as its potential to pro...
Article
Full-text available
Wheat grains are usually low in essential micronutrients. In resolving the problem of grain micronutritional quality, microbe-based technologies, including bacterial endophytes, seem to be promising. Thus, we aimed to (1) isolate and identify grain endophytic bacteria from selected spring wheat varieties (bread Oksamyt myronivs’kyi, Struna myronivs...
Article
Full-text available
Liquid chromatography–tandem mass spectrometry (LC–MS/MS), colorimetry, and bioassays were employed for the evaluation of the ability of endophytic bacterial strains to synthesize indole-related compounds (IRCs) and in particular indole-3-acetic acid (IAA). A total of 54 endophytic strains belonging to seven bacterial genera isolated from tissues o...
Conference Paper
Full-text available
Soil moisture is defined as the water content of the soil. Natural moisture fluctuations associated with the seasons are an environmental factor that regulates microbial activity. However, in recent years, the frequency of floods across Europe has been increasing and drought periods have become longer. The hydrological stress caused by these phenom...
Article
Full-text available
Restoration of ecological functions by reclamation of post-mining areas stimulates soil-formation processes and initiates biological activity on the surfaces of disposal sites. The purpose of this investigation was to study the relationships between soil properties, microbiological activity and diversity in Technosols 45 years following land reclam...
Article
Full-text available
The aims of the study were (1) to recognize the structure of bacteria diversity in Technosols developed from mine spoils containing iron (Fe) sulphides with the use of culture-independent technique, and (2) to determine microbial metabolic activities, in the context of their potential to be an adequate indicators of soil properties being the conseq...
Poster
Full-text available
Wilgotność gleby definiowana jest jako zawartość wody w glebie. Woda w glebie wpływa m.in. na jej odczyn, dyfuzję rozpuszczalników i gazów oraz obieg składników pokarmowych. Umożliwia przemieszczanie się drobnoustrojów, a także stanowi element procesów hydrolizy. Naturalne wahania wilgotności związane z porami roku (opady atmosferyczne) stanowią cz...
Article
Full-text available
The main goal of the study was to determine the biodiversity of bacteria and their metabolic profile in the rhizosphere of four winter wheat (Triticum aestivum L.) varieties (Hondia, Nordkap, Rotax, Tytanika) cultivated in Haplic Podzol soil in a no-tillage system. Two techniques, i.e., next generation sequencing (NGS, meta-barcoding of 16S rRNA co...
Article
Full-text available
Endophytes are associated with host plants throughout their life history from seed germination to fruit development. One of the most important plant organs colonized by endophytic microbiota is the seed. The aim of this study was to determine the structure of the seed core microbiome inhabiting the endosperms and embryos of eight wheat cultivars wi...
Article
Full-text available
The metal hyperaccumulator Azolla filiculoides is accompanied by a microbiome potentially supporting plant during exposition to heavy metals. We hypothesized that the microbiome exposition to selected heavy metals will reveal metal tolerant strains. We used Next Generation Sequencing technique to identify possible metal tolerant strains isolated fr...
Article
Full-text available
Endophytic microbiota plays a role not only in supplying plants with the basic nutrients indispensable for their growth, but also helps them in the mechanisms of adaptation to various environmental stresses (i.e., salinity, drought), which is important in the aspect of crop yields. From the agricultural and biotechnological points of view, the know...
Article
Full-text available
The main goal of the study was to determine the structure of endophytic bacteria inhabiting different parts (endosperm, germ, roots, coleoptiles, and leaves) of two wheat species, Triticum aestivum L. (cv. 'Hondia') and Triticum spelta L. (cv. 'Rokosz'), in order to provide new knowledge about the stability and/or changeability of the core microbio...
Article
Full-text available
The aim of the study was to determine the Actinobacteria structure in cultivated (C) versus non-cultivated (NC) soils divided into three groups (autogenic, hydrogenic, lithogenic) with consideration its formation process in order to assess the Actinobacteria sensitivity to agricultural soil use and soil genesis and to identify factors affecting the...
Article
Full-text available
Microorganisms play an important role in animal nutrition, as they can be used as a source of food or feed. The aim of the study was to determine the nutritional elements and fatty acids contained in the biomass of methanotrophic bacteria. Four bacterial consortia composed of Methylocystis and Methylosinus originating from Sphagnum flexuosum (Sp1),...
Conference Paper
Full-text available
The term endophytic refers to microorganisms that spend the majority or the whole life cycle living within tissue of a host plants. The obligate endophytes exclusively colonize plants and therefore need to be adapted to the host environment. The aim of the study was to recognize the obligatory endophytic microbiome inhabiting inner root and leaves...
Article
Full-text available
The aim of the study was to determine the still not completely described microbiome associated with the aquatic fern Azolla filiculoides. During the experiment, 58 microbial isolates (43 epiphytes and 15 endophytes) with different morphologies were obtained. We successfully identified 85% of microorganisms and assigned them to 9 bacterial genera: A...
Poster
Full-text available
The aim of the study was to recognize the obligatory endophytic microbiome inhabiting inner root and leaves tissues of the two wheat species: Triticum aestivum L. (cv. ‘Hondia’) and T. spelta L. (cv. ‘Rokosz’) under in vitro culture. DNA was isolated according to the procedure dedicated to DNeasy Power Lyzer Power Soil Kit (QIAGEN). Next Generation...
Poster
Full-text available
The aim of the study was to recognize the obligatory endophytic microbiome inhabiting inner root and leaves tissues of the two wheat species: Triticum aestivum L. (cv. ‘Hondia’) and T. spelta L. (cv. ‘Rokosz’) under in vitro culture. DNA was isolated according to the procedure dedicated to DNeasy Power Lyzer Power Soil Kit (QIAGEN). Next Generation...
Article
Full-text available
Methanotrophic bacteria are able to use methane (CH4) as a sole carbon and energy source. Photochemical oxidation of methane takes place in the stratosphere, whereas in the troposphere, this process is carried out by methanotrophic bacteria. On the one hand, it is known that the efficiency of biological CH4 oxidation is dependent on the mode of lan...
Chapter
The knowledge, that biodiversity is an essential factor for maintaining a proper condition of soils is commonly widespread for many years. However, the loss of microbial diversity as an effect of anthropogenic activities still remains in the category of worldwide problem. Until recently our knowledge about an enormous soil biodiversity was strongly...
Article
Full-text available
This study focused on the determination of both catabolic and genetic fingerprinting of bacteria inhabiting soil contaminated with car fuels. A surface layer (0–20 cm) of Mollic Gleysol was used for the experiment and was contaminated with car fuels—unleaded 95-octane petrol and diesel at a dose of 15 g per 10 g of soil. The experiment lasted 42 da...
Article
Microbial coal gasification is of great interest due to the necessity to develop more environmentally friendly methods of energy production. The two main lignite deposits in Poland (Bełchatów and Turów) have been studied for their natural methanogenic potential. The biological origin of gases occurring in the investigated strata was confirmed by ca...
Article
The main goal of the study was to determine new metagenomic indicators demonstrating sensitivity to soil fatigue as an effect of long-term agricultural use as well as new metagenomic indicators demonstrating bacterial resistance to human agricultural activity. Thirty-one soil samples (agriculturally exploited soil and wastelands – serving as contro...
Article
Full-text available
Microbial life in Earth’s deep subsurface is currently known to reach depths of several kilometers within the continental crust. Methanotrophs are a subset of a physiological group of bacteria known as methylotrophs. Phylogenetically, methanotrophs belong to α- or γ- Proteobacteria, but there are also some representatives affiliated as Verrucomicro...
Experiment Findings
Full-text available
The effect of arable soil was quantified against non-cultivated soil on easily degradable carbon and other selected microbiological factors, i.e. soil microbial biomass, respiration activity, and dehydrogenase activity. The intent was to ascertain whether easily degradable carbo can be useful as a sensitive indicator of both soil biological degrada...
Article
Full-text available
The aim of the study was to assess the differences in the bacterial community physiological profiles in soils contaminated with heavy metals versus soils without metal contaminations. The study’s contaminated soil originated from the surrounding area of the Szopienice non-ferrous metal smelter (Silesia Region, Poland). The control was soil unexpose...
Article
Full-text available
The main goal of the study was to find differences in the bacterial community structure resulting from different ways of meadow management in order to get the first insight into microbial biodiversity in meadow samples. The next generation sequencing technique (454-pyrosequencing) was accompanied with the community level physiological profiling (CL...
Article
Full-text available
The aim of the study was to examine the differences in microbial community structure as a result of agricultural practices. Sixteen samples of cultivated and the same number of non-cultivated soils were selected. Gel bands were identified using the GelCompar software to create the presence-absence matrix, where each band represented a bacterial ope...
Article
Full-text available
The main goal of the study was to determine the diversity of the potential nitrogen-fixing (PNF) bacteria inhabiting agricultural (A) soils versus wastelands serving as controls (C). The soils were classified into three groups based on the formation process: autogenic soils (Albic Luvisols, Brunic Arenosols, Haplic Phaeozem) formed on loess materia...
Article
Full-text available
The aim of the study was to demonstrate the impact of soil agricultural usage on the abundance of ammonifying bacteria (AB) and their activity, expressed as arginine ammonification (AA). Five agriculturally exploited types of soils (FAO): Haplic Luvisol, Brunic Arenosol, Mollic Gleysol, Eutric Fluvisol, and Rendzina Leptosol were studied. The contr...
Article
Full-text available
Soil microbial communities play an important role in the biodegradation of different petroleum derivates, including hydrocarbons. Also other biological factors such as enzyme and respiration activities and microbial abundance are sensitive to contamination with petroleum derivates. The aim of this study was to evaluate the response of autochthonic...
Article
Full-text available
Soil biological activity is extremely important for sustaining high soil fertility, crop yields, and therefore can effect economy. Poland is particularly relevant example where approx. 60% of land is used for crop cultivation. It is expected that agricultural soils contain much smaller number of microorganisms relative to non-cultivated soils. We h...
Article
Full-text available
The aim of our study was to analyze the impact of soil agriculture management on bacterial abundance (expressed as total microorganism count plus copiotrophic and oligotrophic bacteria numbers) and soil dehydrogenase activity. Four different soil units agriculturally exploited (under oat-type crops) and the same number of control soils not agricult...
Article
Full-text available
The aim of the study was a statistical evaluation of the impact of selected soil factors − water potential (pF), total organic carbon content (TOC) and land use − on the total DNA content and dehydrogenase activity (DHA) in Mollic Gleysol. Additionally, we wanted to establish the interrelations between two of the most important biological parameter...
Article
Full-text available
Phosphorus is an important life-supporting nutrient and therefore it is often applied in fertilizers. As a result, its pool in soil may increase due to the presence of various elements effectively binding P, making it unavailable to plants. Each soil exhibits many characteristics important in P cycling, with the aeration state (redox potential and...
Article
Full-text available
The study was performed to show how industrial activity affected soil quality in terms of soil DNA quality and quantity as well as soil characteristics. Soil material originated from an urban area of the Silesia Region (SW Poland). The soil characteristics were estimated: texture, moisture, pH, redox potential (Eh), and total carbon content (TOC),...
Article
Full-text available
The purpose of the study was to evidence the phenomenon of soil biological degradation being a result of an intensive soil agricultural practices. This aim is strictly consistent with the recommendations of The European Environmental Agency and Biodiversity Strategy to 2020 strongly promoting monitoring of soil microbiological degradation state in...
Article
Full-text available
Full version to be downloaded up to 50 times: http://www.tandfonline.com/eprint/SRQssShHRxdmP6P4RNwt/full Carbon (C), geologically sequestered in coal, is gradually released to the atmosphere as CH4 and CO2. Recent anthropogenic activity (coal mining) has rapidly increased the rate of C reallocation from coal deposits into the atmosphere, which ha...
Article
Full-text available
Microbial fuel cells (MFCs) are a device using microorganisms as biocatalysts for transforming chemical energy into bioelectricity. As soil is an environment with the highest number of microorganisms and diversity, we hypothesized that it should have the potential for energy generation. The soil used for the study was Mollic Gleysol collected from...
Article
The interplay between soil physical parameters during the recovery from anoxic stresses (reoxidation) is largely unrecognized. This study was conducted to characterise the soil aeration status and derive correlations between variable aeration factors during reoxidation. Surface layers (0–30 cm) of three soil types, Haplic Phaeozem, Mollic Gleysol,...
Article
The interplay between soil physical parameters during the recovery from anoxic stresses (reoxidation) is largely unrecognized. This study was conducted to characterise the soil aeration status and derive correlations between variable aeration factors during reoxidation. Surface layers (0-30 cm) of three soil types, Haplic Phaeozem, Mollic Gleysol,...
Article
Full-text available
The effect of 19-years storage period at air-dried condition (4°C) and impact of soils rewetting on microbial presence were studied. The topsoil (0 to 20 cm) of Mollic Gleysol, Eutric Cambisol, Rendzina Leptosol, Orthic Podzol and Eutric Fluvisol were used in the experiment. It was found that 10-days of soil incubation at full water capacity condit...
Article
Full-text available
The aim of the study was to find the interrelations between the activity of intracellular dehydrogenases, abundance of microorganisms, and the level of soil DNA in the Mollic Gleysol profile, with notification on the dominant DNA form (extra-or intra-cellular), depending on the type of land use. Two neighbouring meadows were selected for investigat...
Article
Full-text available
The paper demonstrates the actual level of chromium contamination at the site of the old tannery waste lagoon in Serniki community (SE Poland). Despite the fact that since 1994 this area is not exploited, the chromium presence is still detected in soil, water and plants. The current study is the 4th phase (after 1989, 2001 and 2004) of checking and...
Article
Full-text available
The paper demonstrates the actual level of chro- mium contamination at the site of the old tan- nery waste lagoon in Serniki community (SE Poland). Despite the fact that since 1994 this area is not exploited, the chromium presence is still detected in soil, water and plants. The cur- rent study is the 4th phase (after 1989, 2001 and 2004) of checki...
Article
Full-text available
The aim of our study was to identify the methanotrophic group of bacteria inhabiting coal mine rocks at the Jastrze{ogonek}bie-Moszczenica (Jas-Mos) coal mine, by application of molecular techniques: polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH). In the first stage of the experiment, methanotrophic activity (AM) of t...