Agnieszka Janiak

• University of Silesia in Katowice

Drought stress can damage crop growth and lead to a decline in yield, thereby affecting food security, especially in regions vulnerable to climate change. SNAC1 (stress-responsive NAC1), the NAC transcription factor family member, plays a crucial role in stomatal movement regulation. Effective regulation of stomatal movement is essential for protec...

Truncated antenna size of photosystems and lower leaf chlorophyll content has been shown to increase photosynthetic efficiency and biomass accumulation in microalgae, cyanobacteria and higher plants grown under high-density cultivation conditions. Here, we have asked whether this strategy is also applicable to a major crop by characterising the bar...

Root hairs play a crucial role in anchoring plants in soil, interaction with microorganisms and nutrient uptake from the rhizosphere. In contrast to Arabidopsis, there is a limited knowledge of root hair morphogenesis in monocots, including barley (Hordeum vulgare L.). We have isolated barley mutant rhp1.e with an abnormal root hair phenotype after...

Purpose Agronomic practices are one of the reasons for the increasing accumulation of elements in the soil, including manganese (Mn). Our previous studies have shown that selenium (Se) ions can reduce the toxic actions of metal stress. Those, we studied the effects of Mn—treated as a stressor and Se – as a potential defense in plants. Methods Mn i...

Purpose Agronomic practices are one of the reasons for the increasing accumulation of elements in the soil, including manganese (Mn). Our previous studies have shown that selenium (Se) ions can reduce the toxic actions of metal stress. Those, we studied the effects of Mn - treated as a stressor and Se – as a potential defense in plants. Methods Mn...

In grasses, biomass and grain production are affected by plant architecture traits such as tiller number, leaf size and orientation. Thus, knowledge regarding their genetic basis is a prerequisite for developing new improved varieties. Mutant screens represent a powerful approach to identify genetic factors underpinning these traits: the Hor TILLUS...

In recent years cultivated soils have been increasingly supplemented with nutrients that at low doses are necessary for proper plant functioning but become toxic at high doses. New methods are needed to prevent these destructive actions, and for this reason we studied the effects of two elements – Mn treated as a stressor and Se treated as a potent...

Balanced photosynthesis under drought is essential for better survival and for agricultural benefits in terms of biomass and yield. Given the current attempts to improve the photosynthetic efficiency for greater crop yield, the explanation of the genetic basis of that process, together with the phenotypic analysis, is significant in terms of both b...

Root systems play a pivotal role in coupling with drought stress, which is accompanied with a substantial transcriptome rebuilding in the root tissues. Here, we present the results of global gene expression profiling of roots of two barley genotypes with contrasting abilities to cope with drought that were subjected to a mild level of the stress. W...

Background: Species of the Festuca and Lolium genera, as well as intergeneric Festuca × Lolium (Festulolium) hybrids, are valuable fodder and turf grasses for agricultural and amenity purposes worldwide. Festulolium hybrids can merge in their genomes agronomically important characteristics. However, in polyploid plants, especially in allopolyploid...

Root hairs are the part of root architecture contributing significantly to the root surface area. Their role is particularly substantial in maintaining plant growth under stress conditions, however, knowledge on mechanism of root hair differentiation is still limited for majority of crop species, including barley. Here, we report the results of a m...

Drought is one of the most adverse abiotic factors limiting growth and productivity of crops. Among them is barley, ranked fourth cereal worldwide in terms of harvested acreage and production. Plants have evolved various mechanisms to cope with water deficit at different biological levels, but there is an enormous challenge to decipher genes respon...

Plant survival in adverse environmental conditions requires a substantial change in the metabolism, which is reflected by the extensive transcriptome rebuilding upon the occurrence of the stress. Therefore, transcriptomic studies offer an insight into the mechanisms of plant stress responses. Here, we present the results of global gene expression p...

The rhizosphere, the thin layer of soil surrounding and influenced by plant roots, defines a distinct and selective microbial habitat compared to unplanted soil. The microbial communities inhabiting the rhizosphere, the rhizosphere microbiota, engage in interactions with their host plants which span from parasitism to mutualism. Therefore, the rhiz...

In the case of the pests inhabiting metal polluted or fields where the use of pesticides is common , a natural selection of resistant individuals can occur. This may pose serious problems for humans, agriculture, as well as the economies of many countries. In this study, the hypothesis that multigenerational (120 generations) exposure to cadmium of...

The knotweed taxa Fallopia japonica, F. sachalinensis and their interspecific hybrid F. × bohemica are some of the most aggressive invaders in Europe and North America and they are serious threats to native biodiversity. At the same time, they constitute a unique model system for the creation of hybrids and studies of the initiation of evolutionary...

An important part of the root system is the root hairs, which play a role in mineral and water uptake. Here, we present an analysis of the transcriptomic response to water deficiency of the wild-type (WT) barley cultivar ‘Karat’ and its root-hairless mutant rhl1.a. A comparison of the transcriptional changes induced by water stress resulted in the...

Stress signalling and regulatory networks controlling expression of target genes are the basis of plant response to drought. Roots are the first organs exposed to water deficiency in the soil and are the place of drought sensing. Signalling cascades transfer chemical signals toward the shoot and initiate molecular responses that lead to the biochem...

Root hairs are tubular outgrowths of specialized epidermal cells called trichoblasts. They affect anchoring plants in soil, the uptake of water and nutrients and are the sites of the interaction between plants and microorganisms. Nineteen root hair mutants of barley representing different stages of root hair development were subjected to detailed m...

In this paper we report on changes in DNA methylation pattern in rape apices and leaves during transition from vegetative to reproductive stage due to grafting and/or vernalization. Grafted plants of winter rape (Brassica napus L., var. "Górczański") (stock from vernalized, scion from non-vernalized plants) were used together with vernalized non-gr...

The spread status of Ambrosia artemisiifolia L. in Poland is still an open problem. The aim of the study was to build a research platform for monitoring the current state of research on A. artemisiifolia allowing the analysis of its present distribution, seed germination, genetic and cytogenetic diversity, the influx of ragweed pollen from neighbor...

The knotweed taxa Fallopia: F. japonica, F. sachalinensis and the hybrid F. bohemica represent a unique model system for the creation of hybrids and the initiation of evolutionary processes in an invaded range. The aim of the study was: (i) to examine the level of genetic diversity in selected populations of Fallopia taxa (Poland, Hungary, Ukraine,...

Recently much attention has been paid to genetic aspects of invasive success in Japanese knotweed s.l. One hypothesis to explain the invasive spread of these species is a multiple introduction, which leads to a higher level of genetic diversity in the invaded range. Fallopia japonica is considered to be genetically uniform in Europe, introduced as...

Recently much attention has been paid to genetic aspects of invasive success in Japanese knotweed s.l. One hypothesis to explain the invasive spread of these species is a multiple introduction, which leads to a higher level of genetic diversity in the invaded range. Fallopia japonica is considered to be genetically uniform in Europe, introduced as...

The mechanisms of root hair formation have been studied extensively in Arabidopsis but knowledge about these processes in monocot species is still limited, especially in relation to the proteome level. The aim of this study was to identify the proteins that are involved in the initiation and the early stage of root hair tip growth in barley using t...

Root hairs are long tubular outgrowths of specialized root epidermal cells that play an important role in plant nutrition and water uptake. They are also an important model in studies of higher plant cell differentiation. In contrast to the model dicot Arabidopsis thaliana, currently very little is known about the genetic and molecular basis of roo...

As the majority of methods for single nucleotide polymorphism (SNP) identification are highly cost-prohibitive, it is necessary to develop new strategies that are more suitable for small and medium-scale laboratories. In this paper, we investigate the potential of degenerate oligonucleotide primed PCR (DOP-PCR) for SNP discovery in soybean. PCR fra...

The effect of selenium (Se) on rape (Brassica napus) seedlings subjected to cadmium (Cd) stress was studied in vitro by investigating plant growth and changes in fatty acid composition, activity of antioxidative enzymes and DNA methylation pattern. Physiological experiments were carried out on seedlings cultured for 2 weeks on Murashige-Scoog (MS)...

In the presented study, the existing AFLP and SSR maps of barley were used to find chromosomal position of four genes controlling different stages of root hair development. Four barley mutants were used in the analysis: the root hairless mutant rhl1.b, mutant rhp1.b with root hair development blocked at the initial bulge formation, mutant rhi1.a wi...

The mutagenic treatment of barley varieties 'Rudzik', 'Dema', 'Krona', 'Diva', 'Karat', and 'Delisa' was performed with the use of N-methylo-N-nitroso-urea (MNU) and sodium azide. To generate high frequency of gene mutations, the combined treatment with both mutagenes (Szarejko and Maluszynski, 1999) or double treatment of MNU was applied. The dose...

Streszczenie: W‡oúniki s" wypustkami komÛrek epidermy korzenia, maj" cylindryczny kszta‡t i charak- teryzuj" siŒ wzrostem szczytowym polegaj"cym na odk‡adaniu nowego materia‡u buduj"cego b‡onŒ i úcianŒ komÛrkow" w szczycie rosn"cej wypustki. Poza zwiŒkszaniem powierzchni ch‡onnej, pobiera- niem wody i soli mineralnych oraz zakotwiczaniem roúliny w...