Agnès Rodrigue

Agnès Rodrigue
Institut National des Sciences Appliquées de Lyon | INSA Lyon · Chemistry-Biology-Health Area (CBS)

About

51
Publications
6,090
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,631
Citations
Citations since 2017
11 Research Items
642 Citations
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120

Publications

Publications (51)
Article
Full-text available
Bacteria, especially those living in soils, are in constant contact with metals. Transition metals like Fe or Zn, are required for proper growth. Some other metals like Cd or Hg are only toxic. Several systems exist to detoxify cells when these metals are present in concentrations harmful to biological systems. The expression of these systems is un...
Article
Full-text available
Few secreted proteins involved in plant infection common to necrotrophic bacteria, fungi and oomycetes have been identified except for plant cell wall-degrading enzymes. Here we study a family of iron-binding proteins that is present in Gram-negative and Gram-positive bacteria, fungi, oomycetes and some animals. Homolog proteins in the phytopathoge...
Article
Full-text available
Identification of the virulence factors of plant pathogenic bacteria has relied on the test of individual mutants on plants, a time‐consuming process. Tn‐seq is a very powerful method for identifying those genes required for bacterial growth in their host. We used this method in a soft‐rot pathogenic bacterium to identify the genes required for the...
Preprint
Full-text available
Soft rot enterobacteria ( Dickeya and Pectobacterium ) are major pathogens that cause diseases on plants of agricultural importance such as potato and ornamentals. Long term studies to identify virulence factors of these bacteria focused mostly on plant cell wall degrading enzymes secreted by the type II secretion system and the regulation of their...
Chapter
Metal ions play key roles in biology. Many are essential for catalysis, for electron transfer and for the fixation, sensing, and metabolism of gases. Others compete with those essential metal ions or have toxic or pharmacological effects. This book is structured around the periodic table and focuses on the control of metal ions in cells. It address...
Article
Full-text available
The use of biosensors as sensitive and rapid alert systems is a promising perspective to monitor accidental or intentional environmental pollution, but their implementation in the field is limited by the lack of adapted inline water monitoring devices. We describe here the design and initial qualification of an analyzer prototype able to accommodat...
Article
Full-text available
Ni is recognized as an element that is toxic to humans, acting as an allergen and a carcinogenic agent, and it is also toxic to plants. The toxicity of Ni has been understudied in microorganisms. The data presented here were obtained by submitting the model bacterium Escherichia coli K-12 to nickel stress. To identify expressed genes, RNA-Seq was p...
Article
The biology of nickel has been widely studied in mammals because of its carcinogenic properties, whereas few studies have been performed in microorganisms. In the present work, changes accompanying stress caused by nickel were evaluated at the cellular level using RNA-Seq in Escherichia coli K-12. Interestingly, a very large number of genes were fo...
Article
Full-text available
Whole-cell biosensors based on reporter genes allow detection of toxic metals in water with high selectivity and sensitivity under laboratory conditions; nevertheless, their transfer to a commercial inline water analyzer requires specific adaptation and optimization to field conditions as well as economical considerations. We focused here on both t...
Article
Full-text available
The detection of nickel in water is of great importance due to its harmfulness for living organism. A way to detect Ni is the use of whole-cell biosensors. The aim of the present work was to build a light-emitting bacterial biosensor for the detection of Ni with high specificity and low detection limit properties. For that purpose, the regulatory c...
Article
Full-text available
The aim of the present work was to engineer bacteria for the removal of Co in contaminated effluents. Radioactive cobalt (60Co) is known as a major contributor for person-sievert budgetary because of its long half-life and high γ- energies. Some bacterial NiCoT genes were described to have preferential uptake for cobalt. In this study the NiCoT gen...
Article
Full-text available
Bacteria require nickel transporters for the synthesis of Ni-containing metalloenzymes in natural, low nickel habitats. In this work we carry out functional and topological characterization of Rhizobium leguminosarum HupE, a nickel permease required for the provision of this element for [NiFe] hydrogenase synthesis. Expression studies in the Escher...
Article
Full-text available
Background Metal contamination is widespread and results from natural geogenic and constantly increasing anthropogenic sources (mainly mining and extraction activities, electroplating, battery and steel manufacturing or metal finishing). Consequently, there is a growing need for methods to detoxify polluted ecosystems. Industrial wastewater, surfac...
Article
Full-text available
Divalent cations play fundamental roles in biological systems where they act as structural and reactive determinants. Their high reactivity with biomolecules has forced living cells to evolve specific pathways for their in vivo handling. For instance the excess of metal can be expelled by dedicated efflux systems. The E. coli RcnA efflux pump expel...
Article
Full-text available
Nickel and cobalt are obligate nutrients for the gammaproteobacteria but when present at high concentrations they display toxic effects. These two metals are present in the environment, their origin being either from natural sources or from industrial use. In this study, the effect of inhibitory concentrations of Ni or Co was assessed on the soil b...
Article
Full-text available
Nickel and cobalt are both essential trace elements that are toxic when present in excess. The main resistance mechanism that bacteria use to overcome this toxicity is the efflux of these cations out of the cytoplasm. RND (resistance-nodulation-cell division)- and MFS (major facilitator superfamily)-type efflux systems are known to export either ni...
Article
RcnA is an efflux pump responsible for Ni and Co detoxification in Escherichia coli. The expression of rcnA is induced by Ni and Co via the metallo-regulator RcnR. In the present work, the functioning of the promoter-operator region of rcnR and rcnA was investigated using primer extension and DNAse I footprinting experiments. We show that the promo...
Article
Full-text available
We report here the identification and characterization of mrdH, a novel chromosomal metal resistance determinant, located in the genomic island 55 of Pseudomonas putida KT2440. It encodes for MrdH, a predicted protein of ∼40 kDa with a chimeric domain organization derived from the RcnA and RND (for resistance-nodulation-cell division) metal efflux...
Article
Full-text available
The survival of bacteria exposed to toxic compounds is a multifactorial phenomenon, involving well-known molecular mechanisms of resistance but also less-well-understood mechanisms of tolerance that need to be clarified. In particular, the contribution of biofilm formation to survival in the presence of toxic compounds, such as nickel, was investig...
Article
Full-text available
Removal of radioactive cobalt at trace levels (approximately nM) in the presence of large excess (10(6)-fold) of corrosion product ions of complexed Fe, Cr, and Ni in spent chemical decontamination formulations (simulated effluent) of nuclear reactors is currently done by using synthetic organic ion exchangers. A large volume of solid waste is gene...
Article
Full-text available
The availability of complete genome sequences of versatile Pseudomonas occupying remarkably diverse ecological niches enabled to gain insights into their adaptative assets. The objective of this study was to analyze the complete genetic repertoires of metal transporters (metal transportomes) from four representative Pseudomonas species and to ident...
Data
Sequence Cluster Analysis for detection of paralogs from four Pseudomonas spp. Description: This data provides evidence for the paralogous nature of metal transporters from various metal transporter families.
Data
Metal transporters exhibiting GI associated features from P.putida KT2440. Description: This table lists out the metal transporters with GI associated features from P.putida KT2440 and provides evidence for the metal transporters with strong and weak GI features.
Data
Catalogue of P.syringae DC3000 metal transporters. Description: This catalogue was compiled for the entire complement of alkali/alkaline earth metal transporters (Na+, K+, Ca2+, Mg2+), transition metal transporters (Zn2+, Mn2+ Cu2+, Ag+, Mo2+, Fe2+, Fe3+, Ni2+, Co2+) and heavy metal transporters (Cr3+, As3+, Pb2+, Cd2+) from P.syringae DC3000. Info...
Data
Metal transporters exhibiting GI associated features from P.aeruginosa PA01. Description: This table lists out the metal transporters with GI associated features from P.aeruginosa PA01 and provides evidence for the metal transporters with strong and weak GI features
Data
Metal transporters exhibiting GI associated features from P.syringae DC3000. Description: This table lists out the metal transporters with GI associated features from P.syringae DC3000 and provides evidence for the metal transporters with strong and weak GI features.
Data
Catalogue of P.aeruginosa PA01 metal transporters. Description: This catalogue was compiled for the entire complement of alkali/alkaline earth metal transporters (Na+, K+, Ca2+, Mg2+), transition metal transporters (Zn2+, Mn2+ Cu2+, Ag+, Mo2+, Fe2+, Fe3+, Ni2+, Co2+) and heavy metal transporters (Cr3+, As3+, Pb2+, Cd2+) from P.aeruginosa PA01. Info...
Data
Metal transporters exhibiting GI associated features from P.fluorescens Pf-5. Description: This table lists out the metal transporters with GI associated features from P.fluorescens Pf-5 and provides evidence for the metal transporters with strong and weak GI features.
Data
Role of HGT in paralogous expansion and acquisition of novel transporter families in Pseudomonas. Description: This table demarcates the role of HGT in paralogous expansion and acquisition of novel transporter families in each of the four Pseudomonas species.
Data
Catalogue of P.putida KT2440 metal transporters. Description: This catalogue was compiled for the entire complement of alkali/alkaline earth metal transporters (Na+, K+, Ca2+, Mg2+), transition metal transporters (Zn2+, Mn2+ Cu2+, Ag+, Mo2+, Fe2+, Fe3+, Ni2+, Co2+) and heavy metal transporters (Cr3+, As3+, Pb2+, Cd2+) from P.putida KT2440. Informat...
Data
Catalogue of P.fluorescens Pf-5 metal transporters. Description: This catalogue was compiled for the entire complement of alkali/alkaline earth metal transporters (Na+, K+, Ca2+, Mg2+), transition metal transporters (Zn2+, Mn2+ Cu2+, Ag+, Mo2+, Fe2+, Fe3+, Ni2+, Co2+) and heavy metal transporters (Cr3+, As3+, Pb2+, Cd2+) from P.fluorescens Pf-5. In...
Conference Paper
Full-text available
The spent decontamination solution is treated, using conventional organic ion exchange resins of the cation and anion type. Removal of cobalt present in trace chemical concentration in the spent decontamination solution has to be achieved in the presence of large excess of corrosion products of Fe, Cr and Ni. However, due to the non-specific sorpti...
Article
Full-text available
The dissociation constant of Ni(II) for Escherichia coli NikR was determined using three independent techniques, including binding kinetics, and shown to be in the sub-micromolar range.
Article
NikR proteins are bacterial metallo-regulatory transcription factors that control the expression of the nickel uptake system and/or nickel containing enzymes such as urease, and are involved in the acid stress response. Here, a comparative study is reported on NikR from Helicobacter pylori (HpNikR) and Escherichia coli (EcNikR), as well as the Q2E...
Article
Amongst the thirty or so two-component systems known in Escherichia coli, the Cpx system has been described as being a stress response system the main function of which is to respond to damage to the cell envelope via activation of proteases and folding catalysts. Nevertheless, the size of the Cpx regulon (several dozens of target genes) and the di...
Article
Full-text available
We report here on the isolation and primary characterization of the yohM gene of Escherichia coli. We show that yohM encodes a membrane-bound polypeptide conferring increased nickel and cobalt resistance in E. coli. yohM was specifically induced by nickel or cobalt but not by cadmium, zinc, or copper. Mutation of yohM increased the accumulation of...
Article
Full-text available
Analysis of a Brucella suis 1330 gene fused to agfp reporter, and identified as being induced in J774 murine macrophage-like cells, allowed the isolation of a gene homologous to nikA, the first gene of the Escherichia coli operon encoding the specific transport system for nickel. DNA sequence analysis of the corresponding B. suis niklocus showed th...
Article
Screening the Pseudomonas aeruginosa genome has led to the identification of the highest number of putative genes encoding two-component regulatory systems of all bacterial genomes sequenced to date (64 and 63 encoding response regulators and histidine kinases, respectively). Sixteen atypical kinases, among them 11 devoid of an Hpt domain, and thre...
Article
Full-text available
Periplasmic or membrane-bound bacterial hydrogenases are generally composed of a small subunit and a large subunit. The small subunit contains a peculiar N-terminal twin-arginine signal peptide, whereas the large subunit lacks any known targeting signal for export. Genetic and biochemistry data support the assumption that the large subunit is cotra...
Article
Full-text available
Bacterial periplasmic nickel-containing hydrogenases are composed of a small subunit containing a twin-arginine signal sequence and a large subunit devoid of an export signal. To understand how the large subunit is translocated into the periplasm, we cloned the hyb operon encoding the hydrogenase 2 ofEscherichia coli, constructed a deletion mutant,...
Article
En conditions de croissance anaérobies, l'entérobactérie Escherichia coli synthétise trois isoenzymes hydrogénase (HYD). HYD1 et HYD2 sont des protéines intégralement membranaires, composées chacune d'une petite et d'une grande sous-unités, tandis que HYD3 est une protéine périphérique de la membrane cytoplasmique. HYD1 et HYD2 catalyse l'oxydation...
Article
The cellular location of membrane-bound NiFe-hydrogenase 2 (HYD2) from Escherichia coli was studied by immunoblot analysis and by activity staining. Treatment of spheroplasts with trypsin was able to release active HYD2 into the soluble fraction, indicating that HYD2 is attached to the periplasmic side of the cytoplasmic membrane and that HYD2 unde...
Article
Full-text available
We analyzed the involvement of chaperonins GroES and GroEL in the biosynthesis of the three hydrogenase isoenzymes, HYD1, HYD2, and HYD3, of Escherichia coli. These hydrogenases are NiFe-containing, membrane-bound enzymes composed of small and large subunits, each of which is proteolytically processed during biosynthesis. Total hydrogenase activity...

Network

Cited By