Agata Kurzyk

• assistant professor at Maria Sklodowska Curie National Research Institute of Oncology

The current cancer research and drug testing are primarily based on 2D cell cultures and animal models. However, these methods have limitations and yield distinct drug response patterns. This study addressed this gap by developing an innovative in vitro human 3D normal skin model and a multicellular model of human cutaneous squamous cell carcinoma...

Hepatocellular carcinoma (HCC) is one of the most threatening tumours in the world today. Pharmacological treatments for HCC mainly rely on protein kinase inhibitors, such as sorafenib and regorafenib. Even so, these approaches exhibit side effects and acquired drug resistance, which is an obstacle to HCC treatment. We have previously shown that se...

Nanohydroxyapatite (nanoHAP) is widely used in bone regeneration, but there is a need to enhance its properties to provide stimuli for cell commitment and osteoconduction. This study examines the effect of calcination at 1200 °C on the physicochemical and biological properties of nanoHAP doped with magnesium (Mg²⁺), strontium (Sr²⁺), and zinc (Zn²⁺...

Evaluation of mesenchymal stem cell seeding efficiency in three-dimensional (3D) scaffolds is a critical step for constructing a potent and useful tissue engineering product for regenerative medicine. To determine the quantity of cells seeded on a scaffold, their condition and viability, and/or to confirm cell adhesion to the scaffold surface, a nu...

The majority of the current cancer research is based on two-dimensional cell cultures and animal models. These methods have limitations, including different expressions of key factors involved in carcinogenesis and metastasis, depending on culture conditions. Addressing these differences is crucial in obtaining physiologically relevant models. In t...

The majority of the current cancer research is based on two-dimensional cell cultures and animal models. These methods have limitations, including different expression of key factors involved in carcinogenesis and metastasis, depending on culture conditions. Addressing these differences is crucial in obtaining physiologically relevant models. In th...

Biodruki to nie tylko przyszłość medycyny regeneracyjnej, lecz także szansa na precyzyjniejsze i etyczne badania naukowe.

3D bioprinting not only represents the future of regenerative medicine, it can also facilitate more precise and more ethical scientific research.

The study examines the effect of calcination at a temperature of 1200°C on the physicochemical and biological properties of nanohydroxyapatite (nanoHAP) substituted with magnesium (Mg ²⁺ ), strontium (Sr ²⁺ ), and zinc (Zn ²⁺ ). The materials were characterized by Fourier-transform spectroscopy, X-ray diffraction (XRD), scanning electron microscopy...

Background: Vascularization is important for the clinical application of tissue engineered products. Both adipose-derived stem cells (ASCs) and surgical prefabrication can be used to induce angiogenesis in scaffolds. Our aim was to compare the angiogenic potential of ASC-seeded scaffolds combined with scaffold prefabrication with that of non-seede...

The purpose of the current study was to evaluate the usefulness of adipose-derived stem cells (ASCs) for bone injury therapy. Lipoaspirates were collected from the abdomen regions of 17 healthy female donors (mean age 49 ± 6 years) using Coleman technique or Body-jet liposuction. In the present study, the primary objective was the in vitro characte...

Adipose tissue yields adult adipose stem cells (ASCs) in large quantities via less-invasive methods. These cells are of interest owing to their modulating properties and paracrine activities, which can be harnessed in regenerative medicine. Many studies on the use of rat fat tissue in an autologous animal model have been conducted; however, the dif...

Angiogenesis is the formation of new blood vessels from existing vessels. This process occurs via budding endothelial cells in postnatal period, which is essential to many physiological phenomena (e.g. wound healing, formation of the placenta) and pathological ones such as cancer, ischemic diseases, and chronic inflammation. Various mechanisms of t...

Artykuł otrzymano 25 sierpnia 2014 r. Artykuł zaakceptowano 5 listopada 2014 r. Słowa kluczowe: angiogeneza, śródbłonek, czynnik proangiogenny, czynnik antyan-giogenny, antyangiogenna terapia, nowo-twór, komórki macierzyste Wykaz skrótów: AML (ang. acute myelo-id leukemia) — ostra białaczka szpikowa; FGF (ang. fibroblast growth factor) — czyn-nik w...

The multipotential progenitor cells called ,Mesenchymal Stem Cells" (MSC) are capable of differrentiation at least into bone, cartilage, and adipose tissues. The commonly recognized role of these cells is the formation of connective tissue which participates in formation of every organ. The progeny of MSC produces also the hematopoietic microenviro...

High light induces the production of cysteine and glutathione (GSH) as part of the oxidative stress response triggered by reactive oxygen species. Cysteine is produced in a two-step process, in which serine acetyltransferase (SAT) catalyses the first reaction. In Arabidopsis thaliana cyclophilin CYP20-3 was reported to promote plastidic SAT1 activi...

Glioblastoma multiforme is the most common type of primary brain tumor in adults. WWOX is a tumor suppressor gene involved in carcinogenesis and cancer progression in many different neoplasms. Reduced WWOX expression is associated with more aggressive phenotype and poor patient outcome in several cancers. We investigated alternations of WWOX expres...

Glioblastoma multiforme is the most common type of primary brain tumor in adults. WWOX is a tumor suppressor gene involved in carcinogenesis and cancer progression in many different neoplasms. Reduced WWOX expression is associated with more aggressive phenotype and poor patient outcome in several cancers. We investigated alternations of WWOX expres...