Adriano Brandelli

Federal University of Rio Grande do Sul | UFRGS · Departamento de Ciência dos Alimentos

Salmonella contamination in poultry products remains a significant concern worldwide, affecting food safety and public health. In this study, carvacrol encapsulated in chia mucilage nanocapsules (CMNP) was tested against a cocktail of Salmonella enterica artificially inoculated into chicken meat. The Bactericidal Inactivation Concentration (BIC) of...

Aryl polyene (APE) are bacterial pigments which show great biotechnological potential because of their biological activities. In this study, the presence of gene clusters associated with APE synthesis was investigated in the genome of Chryseobacterium sp. kr6 and Lysobacter sp. A03. The pigments extracted from strains kr6 and A03 were further chara...

O soro de leite é considerado um co produto da indústria de laticínios. Gerado em grandes quantidades, é considerado um efluente de impacto ambiental significativo, pois possui um alto poder poluente, necessitando de um manejo adequado. Contudo, o soro de leite também é uma boa fonte de nutrientes, como proteínas, lactose e minerais. Diversos são o...

Microbial fermentation represents an interesting strategy for the management and valorization of agro-industrial byproducts. In this study, the proteolytic strain Bacillus sp. CL18 was used to produce bioactive hydrolysates during submerged cultivation with various protein-containing substrates, including byproducts from the poultry (feathers), che...

This study investigates the antifungal potential of encapsulated essential oil (EO) from Baccharis dracunculifolia and nerolidol (NE) within Pluronic® F-127 nanoparticles (NPs). The EO, containing nerolidol, β-caryophyllene, and α-pinene as major bioactive compounds, exhibited superior antifungal activity compared to NE. The NP-EO formulations demo...

Cabbage is susceptible to various microbiological risks, frequently serving as a vehicle for pathogenic bacteria, mainly Salmonella and Escherichia coli. Therefore, ensuring the safety of this vegetable is essential to reduce the risk of foodborne illnesses. Traditional sanitization using chlorinated water, although effective, raises concerns due t...

Bioactive keratin hydrolysates obtained from microbial treatment of poultry feathers were incorporated into polycaprolactone (PCL) nanofibers using the electrospinning method. The nanofiber mats were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, thermal analysis, and hemolysis rate. Feather ker...

In this study, lactobionic acid (LBA) was incorporated into poly(vinyl alcohol) (PVA) and poly(ε-caprolactone) (PCL) by electrospinning. The antimicrobial effects of the nanofibers were tested using the agar diffusion method. Only the PVA formulations showed antimicrobial activity against Staphylococcus aureus. The PVA and PCL nanofibers containing...

The high content and quality of protein in Andean legumes make them valuable for producing protein hydrolysates using proteases from bacteria isolated from extreme environments. This study aimed to carry out a single-step purification of a haloprotease from Micrococcus sp. PC7 isolated from Peru salterns. In addition, characterize and apply the enz...

This manuscript describes the development and characterization of electrospun nanofibers incorporating bioactive hydrolysates obtained from the microbial bioconversion of feathers, a highly available agro-industrial byproduct. The electrospun nanofibers were characterized using different instrumental methods, and their antioxidant properties and to...

There are massive sources of lactic acid bacteria (LAB) in traditional dairy products. Some of these indigenous strains could be novel probiotics with applications in human health and supply the growing needs of the probiotic industry. In this work, were analyzed the probiotic and technological properties of three Lactobacilli strains isolated from...

Obtaining natural pigments from microorganisms is an alternative with high potential for biotechnological application. The use of agro-industrial wastes as substrate for cultivations enables a reduction of the production cost and may add value to potentially polluting byproducts. In this work, the extraction of pigments produced by the bacterium Ch...

Lysobacter is known as a bacterial genus with biotechnological potential, producing an array of enzymes, antimicrobial metabolites, and bioactive antioxidant compounds, including aryl polyene (APE) pigments that have been described as protecting substances against photooxidative damage and lipid peroxidation. In this study, the pigment extracted fr...

The present work aimed to evaluate the features of yerba mate kombucha during 7 days of fermentation at either 25 ºC or 30 ºC, monitoring physicochemical changes, sensory profile, and sensorial acceptance. The symbiotic microbial culture of active bacteria and yeasts (SCOBY) at the beginning and the end of the bioprocess was also identified. The ye...

The advances in nanocomposites incorporating bioactive substances have the potential to transform the food packaging sector. Different nanofillers have been incorporated into polymeric matrixes to develop nanocomposite materials with improved mechanical, thermal, optical and barrier properties. Nanoclays, nanosilica, carbon nanotubes, nanocellulose...

Pseudomonas sp. 4B isolated from the effluent pond of a bovine abattoir was investigated as antifungal against toxigenic fungi. The complete genome of Pseudomonas 4B was sequenced using the Illumina MiSeq platform. Phylogenetic analysis and genome comparisons indicated that the strain belongs to the Pseudomonas aeruginosa group. In silico investiga...

BACKGROUND Products fermented with lactic acid bacteria based on whole grain flours of red or white sorghum (Sorghum bicolor (L.) Moench) added with malted sorghum flour, or with skim milk (SM) were developed. Composition, protein amino acid profile, total acidity, pH, prebiotic potential, and bio‐functional properties after simulation of gastroint...

Nowadays, there is a great interest on rapid and effective methods for initial identification of probiotic bacteria. In this work, potential probiotic features of the lactic acid bacteria strain ItalPN16 isolated from a traditional Brazilian cheese were studied using bioinformatic tools. The complete genome sequence was obtained, and in silico anal...

The gut microbiome plays a critical role to all animals and humans health. Methods based on ex vivo cultures are time and cost-effective solutions for rapid evaluation of probiotic effects on microbiomes. In this study, we assessed whether the protein secretome from the potential probiotic Enterococcus durans LAB18S grown on fructoligosaccharides (...

This article aims to present an updated discussion about the relevant aspects of SARS-CoV-2 transmission in food systems and challenges imposed by the COVID-19 pandemic for food industries. To accomplish this review, data from relevant papers describing the impacts of the COVID-19 pandemic on food security were retrieved from selected databases. Th...

The production of probiotic bacteria requires specific and expensive culture media for maintain their viability and metabolic response during gastro-intestinal transit and cell adhesion process. The aim of this study was to compare the ability of the potential probiotic Laticaseibacillus paracasei ItalPN16 to grow in plain sweet whey (SW) and acid...

In the food sector, one of the most important economic activities is the dairy industry, which has been facing many challenges in order to meet the increasing demand by consumers for natural and minimally processed products with high quality. In this sense, the application of innovative and emerging technologies can be an interesting alternative, f...

This study aimed to purify and partially characterize a keratinolytic protease produced by Bacillus sp. P45 through bioconversion of feather meal. Crude protease extract was purified using a sequence of an aqueous two-phase system (ATPS) in large volume systems (10, 50, and 500 g) to increase obtaining purified enzyme, followed by a diafiltration (...

Jaboticaba peels are an important source of health-benefit and antimicrobial compounds. The present work aims to evaluate concentration of polyphenolics and the mode of action of aqueous extract from jaboticaba peels against Staphylococcus aureus and Escherichia coli. Jaboticaba peel extract showed minimum inhibitory concentration and minimum bacte...

Bacillus-based biocontrol agents have emerged as a strategy to eliminate or reduce the use of synthetic fungicides that are detrimental to health and the environment. In vineyards, a special concern arises from the control of Aspergillus carbonarius, a fungus known for its potential to produce ochratoxins. Ochratoxin A (OTA) is the most toxic form...

Marine pollution has been increasing over the years and can impact directly living organisms. The continued pollution of soil and fresh water by agriculture, industrial and urban activities frequently reaches the rivers and the ocean by run over polluting from these environments. Bioremediation is an eco-friendly technique that can immobilize, redu...

Liposomes are among the most studied nanostructures. They are effective carriers of active substances both in the clinical field, such as delivering genes and drugs, and in the food industry, such as promoting the controlled release of bioactive substances, including food preservatives. However, toxicological screenings must be performed to ensure...

Encapsulation can be an effective strategy to protect food additives and nutraceuticals against some harsh conditions encountered during food processing, and provide efficient incorporation of antimicrobials and beneficial microorganisms in food matrices. Liposomes are suitable systems for delivery and controlled release of natural antimicrobial su...

Enzymatic hydrolysis is the most prominent strategy to release bioactive peptides from different food proteins and protein-rich by-products. Unconventional microbial proteases (UMPs) have gaining increased attention for such purposes, particularly from the 2010s. In this review, we present and discuss aspects related to UMPs production, and their u...

Recycling of dairy by-products as sources of bioactive compounds is an interesting approach for improving sustainable food production. This study aimed to encapsulate a sheep whey hydrolysate (SWH) with antioxidant and antihypertensive properties, produced by a novel proteinase P7. SWH encapsulation was achieved by spray-drying and freeze-drying me...

A physical-mathematical model was used to evaluate the capability of an enzymatic pool of Bacillus sp. P7 (isolated from Piaractus mesopotamicus) to promote the bovine casein micelles coagulation. Experiments were designed to assess the effects of temperature, pH, and enzyme activity/mass of substrate ratio on the kinetic parameters of the coagulat...

Feather is the most abundant keratinous material in nature. This by-product of animal origin has about 80–90% of crude protein. This work aimed to maximize the enzymatic hydrolysis of feather meal using the proteolytic enzyme from Bacillus sp. P45 and evaluate the antioxidant capacity of different fractions of the hydrolysate. Enzymatic hydrolysis...

Several biological activities have been related to peptides derived from food proteins. Short peptides encrypted within the original protein sequence can be released by the action of proteolytic enzymes, exerting several biological activities that can be useful in food preservation and as nutraceutical compounds. In this study, bovine whey hydrolys...

Carvacrol is a natural antimicrobial with excellent antimicrobial properties against several foodborne pathogens. Encapsulation can increase carvacrol stability and solubility, and mask its pronounced odor. Mucilages have been studied as wall material for nanoparticles due to their high retention capacity of bioactive compounds and ease of chemical...

With the onset of Listeria monocytogenes resistance to the bacteriocin nisin, the search for alternative antimicrobial treatments is of fundamental importance. In this work, we set out to investigate proteins and lipids involved in the resistance mechanisms of L. monocytogenes against the antimicrobial peptides (AMPs) nisin and fengycin. The effect...

The cellular proteins of L. monocytogenes exposed to free and liposome-encapsulated nisin at sublethal concentration were hydrolyzed by trypsin and examined by tandem mass spectrometry (MS/MS) to obtain proteomic data. In the present study, we use the STRING v11.05 database analyze the interactions among the 78 upregulated proteins from L. monocyto...

Beer production generates a large amount of waste, called brewers’ spent grain (BSG), which has a nutritional potential mainly as a protein source. Proteins can be modified by hydrolysis releasing peptides with different bioactivities, such as antioxidant activity. The aim of this study was to produce a protein hydrolysate from protein concentrate...

Gut microbiota exerts a fundamental role in human health and increased evidence supports the beneficial role of probiotic microorganisms in the maintenance of intestinal health. Enterococcus durans LAB18S was previously isolated from soft cheese and showed some desirable in vitro probiotic properties, for that reason its genome was sequenced and ev...

Nisin is an antimicrobial peptide broadly used as a preservative in the food industry. In thisstudy, oil-core polymeric nanocapsules encapsulating nisin were prepared by a nanoprecipitation technique using three different synthetic biodegradable polymers, namely poly(butylene adipate-coterephthalate) (PBAT), Eudragit RS-100® (EUD), and poly(ε-capro...

Probiotics are microorganisms capable to promote health benefits to the host. This chapter presents an introduction to the history of fermented foods and probiotics, as well as the probiotics concept and beneficial properties to human health, relationship with the human intestinal microbiome, global market, food safety and regulatory issues and mai...

Dietary ingredients can rapidly modify the composition of the gut microbiota and subsequently influence the host health. The dysbiosis of gut microbiome has been associated with several diseases and many studies indicate that probiotics and prebiotics can work on the prevention of food-related lifestyle illnesses. The ingestion of functional food i...

The development of high-throughput technologies permitted the transition of biology studies from laboratory-based hypothesis to holistic approaches. The so-called omics methodologies analyze and integrate information coming from different steps in the central dogma of molecular biology, such as genomics, transcriptomics, proteomics, metabolomics, a...

In the last years, the scientific community has recognized that specific microbial strains resident in the intestinal ecosystem play a key role in human health, participating in several functions beneficial to the host. Such microorganisms have been termed as next-generation probiotics and they are presently considered as food/nutraceutical supplem...

Feathers are naturally made up of non-digestible proteins. Under thermal processing, total tract digestibility can be partially improved. Furthermore, Bacillus subtilis (Bs) has shown a hydrolytic effect In vitro. Then, a Bs FTC01 was selected to hydrolyze enough feathers to produce a meal, and then test the quality and inclusion in the dog’s diet...

Aims: To investigate the potential of novel Bacillus velezensis P45 as an eco-friendly alternative for bioprocessing poultry by-products into valuable antimicrobial products. Methods and results: The complete genome of B. velezensis P45 was sequenced using the Illumina MiSeq platform, showing 4,455 protein and 98 RNA coding sequences according t...

The objective of this work was to evaluate the concentration of phenolic compounds, the antioxidant and anti-hypertensive capacity, and the physicochemical characteristics of brewer’s spent grain (BSG) from Pilsen, Red Ale and Stout beers. Results indicate that the studied byproducts present high concentration of total polyphenols, flavonols and ph...

Bacteria of the genus Bacillus sp. present the potential for inhibiting various pathogens, making them a promising starting point in the search for new antimicrobial substances. In this study, bacteria were isolated from sediment samples from humid areas of a Natural Conservation Unit in the state of Rio Grande do Sul, Brazil. The isolate Bacillus...

The aim of this study was to investigate the combined effect of carvacrol, thymol and nisin against Staphylococcus aureus and the combined effect of carvacrol and thymol against Salmonella Enteritidis. Minimum inhibitory concentrations (MIC) of carvacrol, thymol, and nisin for S. aureus were 200, 150 and 30 µg/mL, respectively. MIC of carvacrol and...

Purpose This study aimed to evaluate the antimicrobial activity of the aqueous extract from jaboticaba skin against important foodborne bacteria and fungi and its stability. Design/methodology/approach Jaboticaba skin aqueous extract (at ratio of 10 g L-1) was tested against Listeria monocytogenes , Staphylococcus aureus , Bacillus cereus and Esch...

Bacillus spp. have been used as a biocontrol strategy to eliminate/reduce toxic fungicides in viticulture. Furthermore, the presence of fungi that are resistant to commonly used products is frequent, highlighting the need for new biocontrol strains. Aspergillus carbonarius can produce ochratoxins, including ochratoxin A (OTA), which has a regulator...

The identification of antioxidant, antidiabetogenic and antimicrobial peptides obtained from sorghum spent grain (SSG) protein hydrolysate was performed. Antioxidant activity (AOA) was evaluated by ABTS⁺ radical cation scavenging, antidiabetogenic activity (ADA) by dipeptidyl peptidase IV (DPP-IV) inhibition, and antimicrobial activity (AMA) throug...

Resumo O objetivo deste trabalho foi desenvolver formulações de misturas para preparo de bolos de caneca, sem ovos e sem leite, com farinhas de cascas e sementes de uvas, além de avaliar características físico-químicas, tecnológicas e sensoriais, com enfoque nos efeitos dos ácidos ascórbico e cítrico sobre os bolos, com substituição parcial da fari...

The objective of the present work was to evaluate polyphenols, trace elements and compounds with antioxidant, antimicrobial and anti-hypertensive activity in wild strawberries cultivated in Southern Brazil. Fruits present high fiber, iron, potassium and sodium concentrations. Polyphenolic content was 242.12 mg of gallic acid equivalent per 100g and...

Wetlands are ecosystems rich in biodiversity and their ecological importance is recognized worldwide. Sediment samples were subjected to physical-chemical analysis and organic carbon content varied from 3.0% to 4.8%, the clay between 32 and 40%, silt with 41% and 43%, sand coarse varied between 6 and 11% and fine sand between 7 and 16%. The nitroge...

Nisin was encapsulated in silica through sol-gel process by acid-catalyzed routes. The silica xerogels were characterized through nitrogen adsorption isotherms, small-angle X-ray scattering (SAXS), zeta potential, X-ray diffraction (XRD), scanning electron microscopy (SEM), diffuse reflectance spectroscopy (DRS), and Fourier transform infrared spec...

Encapsulation can be a suitable strategy to protect natural antimicrobial substances against some harsh conditions of processing and storage and to provide efficient formulations for antimicrobial delivery. Lipid-based nanostructures, including liposomes, solid lipid nanoparticles (SLNs), and nanostructured lipid nanocarriers (NLCs), are valuable s...

The objective of the present study was to obtain and evaluate the conditions of acid hydrolysis for cellulose nanocrystals (CNC) production, as well as to characterize its chemical and morphological structure and finally to realize and evaluate its application potential on paper as a structural reinforcing agent. From bleached cellulose, CNC is obt...

Background The current relevance of functional foods in human health and well-being has led to an increased interest in developing new types of oral administration systems for probiotics and nutraceuticals. Encapsulation may provide protection and sustained delivery in food formulations. Scope and approach The recent discussions about probiotics e...

Objectives The co-encapsulation of bioactive peptides obtained from degradation of chicken feathers and flexirubin-type pigment produced by Chryseobacterium sp. kr6 into phosphatidylcholine liposomes was investigated. Results Control empty liposomes showed mean diameter of 168.5 nm, varying to 185.4, 102.0 and 98.5 nm after the encapsulation of pe...

The ability of Listeria monocytogenes grow on ready-to-eat food is a major concern in food safety. Natural antimicrobials, such as nisin, can be used to control this pathogen, but the increasing reports of nisin tolerance and resistance make necessary novel approaches to increase its effectiveness, such as encapsulation. The goal of this study was...

In this work, the effect of antimicrobial lipopeptide P34 on Listeria monocytogenes was evaluated for the first time through a proteomics approach. Bacteria were treated with sublethal doses of peptide P34 (F-P34) and P34 encapsulated into nanoliposomes (N-P34), while empty nanoliposomes (NE) and fresh buffer were used as controls. The proteomic an...

A great diversity of biological activities has been attributed to peptides. Despite food proteins are essentially considered as sources of dietary amino acids, bioactive peptides can be released during gastrointestinal transit, food fermentation, food maturation, and through enzymatic bioprocessing in vitro. Specific bioactive peptide databases int...

Recently, renewable and biocompatible polymers have gained increased relevance for food packaging applications. The use of nanostructures for the development of active and intelligent bio-nanocomposite packaging can be beneficial for food safety and quality. Natural polymers, such as chitosan, starch, cellulose and its derivatives, poly(lactic acid...

The antimicrobial peptide produced by Bacillus velesensis P34 has a broad activity against Gram-positive bacteria, showing potential as natural food preservative. In this work, nanocapsules (NCs) containing the peptide P34 were produced using the polymers poly-ε-caprolactone (PCL) or Eudragit RS-100 (EUD), and their antimicrobial activities were as...

Amino acids (AAs) and proteins are major macronutrients in feedstuffs and fish. Due to a paucity of information about tissue-specific AA nutrition in aquatic animals, this review highlights the important energetic roles and functions of AAs (particularly glutamate, glutamine, and aspartate) in some tissues (particularly skeletal muscle, liver, kidn...

The interactions between liposomes and fish myofibrillar protein (surimi ground salted protein, SURP) were evaluated. Liposomes prepared with ultrapure phosphatidylcholine (UPC) or partially purified phosphatidylcholine (PPC) were dispersed at different weight ratio on SURP. Changes in protein stability and structure were evaluated using FTIR, intr...

The antimicrobial activities of Baccharis dracunculifolia DC essential oil (EO) and hydroalcoholic extract (HE) were evaluated. The EO showed broad antimicrobial activity and its synergistic combination with nisin was tested. Major components of EO were nerolidol, beta-pinene and D-limonene, while artepillin C, rutin and cafeic acid were major phen...

Liposomes modified with biomolecules can present better stability and specific structural characteristics, representing a profitable alternative to conventional liposomes. In this study, liposomes formulations containing pectin (PT) or polygalacturonic acid (PA) were prepared by the thin-film hydration method and reverse phase evaporation method fo...

The world demands new solutions and products to be used as dyes for industrial applications. Microbial pigments represent an eco-friendly alternative as they can be produced in large amounts through biotechnological processes and do not present environmental risks, as they are easily decomposable. Moreover, some of these metabolites are recognized...

The elimination of microbial surface contaminants is one of the most important steps in Good Manufacturing Practices in order to maintain food safety. This is usually achieved by detergents and chemical sanitizers, although an increased demand exists for the use of natural p