Adriano Brandelli

Universidade Federal do Rio Grande do Sul | UFRGS · Departamento de Ciência dos Alimentos

This study aimed to purify and partially characterize a keratinolytic protease produced by Bacillus sp. P45 through bioconversion of feather meal. Crude protease extract was purified using a sequence of an aqueous two-phase system (ATPS) in large volume systems (10, 50, and 500 g) to increase obtaining purified enzyme, followed by a diafiltration (...

Jaboticaba peels are an important source of health-benefit and antimicrobial compounds. The present work aims to evaluate concentration of polyphenolics and the mode of action of aqueous extract from jaboticaba peels against Staphylococcus aureus and Escherichia coli. Jaboticaba peel extract showed minimum inhibitory concentration and minimum bacte...

Bacillus-based biocontrol agents have emerged as a strategy to eliminate or reduce the use of synthetic fungicides that are detrimental to health and the environment. In vineyards, a special concern arises from the control of Aspergillus carbonarius, a fungus known for its potential to produce ochratoxins. Ochratoxin A (OTA) is the most toxic form...

Marine pollution has been increasing over the years and can impact directly living organisms. The continued pollution of soil and fresh water by agriculture, industrial and urban activities frequently reaches the rivers and the ocean by run over polluting from these environments. Bioremediation is an eco-friendly technique that can immobilize, redu...

Liposomes are among the most studied nanostructures. They are effective carriers of active substances both in the clinical field, such as delivering genes and drugs, and in the food industry, such as promoting the controlled release of bioactive substances, including food preservatives. However, toxicological screenings must be performed to ensure...

Encapsulation can be an effective strategy to protect food additives and nutraceuticals against some harsh conditions encountered during food processing, and provide efficient incorporation of antimicrobials and beneficial microorganisms in food matrices. Liposomes are suitable systems for delivery and controlled release of natural antimicrobial su...

Enzymatic hydrolysis is the most prominent strategy to release bioactive peptides from different food proteins and protein-rich by-products. Unconventional microbial proteases (UMPs) have gaining increased attention for such purposes, particularly from the 2010s. In this review, we present and discuss aspects related to UMPs production, and their u...

Recycling of dairy by-products as sources of bioactive compounds is an interesting approach for improving sustainable food production. This study aimed to encapsulate a sheep whey hydrolysate (SWH) with antioxidant and antihypertensive properties, produced by a novel proteinase P7. SWH encapsulation was achieved by spray-drying and freeze-drying me...

A physical-mathematical model was used to evaluate the capability of an enzymatic pool of Bacillus sp. P7 (isolated from Piaractus mesopotamicus) to promote the bovine casein micelles coagulation. Experiments were designed to assess the effects of temperature, pH, and enzyme activity/mass of substrate ratio on the kinetic parameters of the coagulat...

Feather is the most abundant keratinous material in nature. This by-product of animal origin has about 80–90% of crude protein. This work aimed to maximize the enzymatic hydrolysis of feather meal using the proteolytic enzyme from Bacillus sp. P45 and evaluate the antioxidant capacity of different fractions of the hydrolysate. Enzymatic hydrolysis...

Several biological activities have been related to peptides derived from food proteins. Short peptides encrypted within the original protein sequence can be released by the action of proteolytic enzymes, exerting several biological activities that can be useful in food preservation and as nutraceutical compounds. In this study, bovine whey hydrolys...

Carvacrol is a natural antimicrobial with excellent antimicrobial properties against several foodborne pathogens. Encapsulation can increase carvacrol stability and solubility, and mask its pronounced odor. Mucilages have been studied as wall material for nanoparticles due to their high retention capacity of bioactive compounds and ease of chemical...

With the onset of Listeria monocytogenes resistance to the bacteriocin nisin, the search for alternative antimicrobial treatments is of fundamental importance. In this work, we set out to investigate proteins and lipids involved in the resistance mechanisms of L. monocytogenes against the antimicrobial peptides (AMPs) nisin and fengycin. The effect...

The cellular proteins of L. monocytogenes exposed to free and liposome-encapsulated nisin at sublethal concentration were hydrolyzed by trypsin and examined by tandem mass spectrometry (MS/MS) to obtain proteomic data. In the present study, we use the STRING v11.05 database analyze the interactions among the 78 upregulated proteins from L. monocyto...

Beer production generates a large amount of waste, called brewers’ spent grain (BSG), which has a nutritional potential mainly as a protein source. Proteins can be modified by hydrolysis releasing peptides with different bioactivities, such as antioxidant activity. The aim of this study was to produce a protein hydrolysate from protein concentrate...

Gut microbiota exerts a fundamental role in human health and increased evidence supports the beneficial role of probiotic microorganisms in the maintenance of intestinal health. Enterococcus durans LAB18S was previously isolated from soft cheese and showed some desirable in vitro probiotic properties, for that reason its genome was sequenced and ev...

Nisin is an antimicrobial peptide broadly used as a preservative in the food industry. In thisstudy, oil-core polymeric nanocapsules encapsulating nisin were prepared by a nanoprecipitation technique using three different synthetic biodegradable polymers, namely poly(butylene adipate-coterephthalate) (PBAT), Eudragit RS-100® (EUD), and poly(ε-capro...

Probiotics are microorganisms capable to promote health benefits to the host. This chapter presents an introduction to the history of fermented foods and probiotics, as well as the probiotics concept and beneficial properties to human health, relationship with the human intestinal microbiome, global market, food safety and regulatory issues and mai...

Dietary ingredients can rapidly modify the composition of the gut microbiota and subsequently influence the host health. The dysbiosis of gut microbiome has been associated with several diseases and many studies indicate that probiotics and prebiotics can work on the prevention of food-related lifestyle illnesses. The ingestion of functional food i...

The development of high-throughput technologies permitted the transition of biology studies from laboratory-based hypothesis to holistic approaches. The so-called omics methodologies analyze and integrate information coming from different steps in the central dogma of molecular biology, such as genomics, transcriptomics, proteomics, metabolomics, a...

In the last years, the scientific community has recognized that specific microbial strains resident in the intestinal ecosystem play a key role in human health, participating in several functions beneficial to the host. Such microorganisms have been termed as next-generation probiotics and they are presently considered as food/nutraceutical supplem...

Feathers are naturally made up of non-digestible proteins. Under thermal processing, total tract digestibility can be partially improved. Furthermore, Bacillus subtilis (Bs) has shown a hydrolytic effect In vitro. Then, a Bs FTC01 was selected to hydrolyze enough feathers to produce a meal, and then test the quality and inclusion in the dog’s diet...

Aims: To investigate the potential of novel Bacillus velezensis P45 as an eco-friendly alternative for bioprocessing poultry by-products into valuable antimicrobial products. Methods and results: The complete genome of B. velezensis P45 was sequenced using the Illumina MiSeq platform, showing 4,455 protein and 98 RNA coding sequences according t...

Bacteria of the genus Bacillus sp. present the potential for inhibiting various pathogens, making them a promising starting point in the search for new antimicrobial substances. In this study, bacteria were isolated from sediment samples from humid areas of a Natural Conservation Unit in the state of Rio Grande do Sul, Brazil. The isolate Bacillus...

The aim of this study was to investigate the combined effect of carvacrol, thymol and nisin against Staphylococcus aureus and the combined effect of carvacrol and thymol against Salmonella Enteritidis. Minimum inhibitory concentrations (MIC) of carvacrol, thymol, and nisin for S. aureus were 200, 150 and 30 µg/mL, respectively. MIC of carvacrol and...

Purpose This study aimed to evaluate the antimicrobial activity of the aqueous extract from jaboticaba skin against important foodborne bacteria and fungi and its stability. Design/methodology/approach Jaboticaba skin aqueous extract (at ratio of 10 g L-1) was tested against Listeria monocytogenes , Staphylococcus aureus , Bacillus cereus and Esch...

Bacillus spp. have been used as a biocontrol strategy to eliminate/reduce toxic fungicides in viticulture. Furthermore, the presence of fungi that are resistant to commonly used products is frequent, highlighting the need for new biocontrol strains. Aspergillus carbonarius can produce ochratoxins, including ochratoxin A (OTA), which has a regulator...

The identification of antioxidant, antidiabetogenic and antimicrobial peptides obtained from sorghum spent grain (SSG) protein hydrolysate was performed. Antioxidant activity (AOA) was evaluated by ABTS⁺ radical cation scavenging, antidiabetogenic activity (ADA) by dipeptidyl peptidase IV (DPP-IV) inhibition, and antimicrobial activity (AMA) throug...

Resumo O objetivo deste trabalho foi desenvolver formulações de misturas para preparo de bolos de caneca, sem ovos e sem leite, com farinhas de cascas e sementes de uvas, além de avaliar características físico-químicas, tecnológicas e sensoriais, com enfoque nos efeitos dos ácidos ascórbico e cítrico sobre os bolos, com substituição parcial da fari...

The objective of the present work was to evaluate polyphenols, trace elements and compounds with antioxidant, antimicrobial and anti-hypertensive activity in wild strawberries cultivated in Southern Brazil. Fruits present high fiber, iron, potassium and sodium concentrations. Polyphenolic content was 242.12 mg of gallic acid equivalent per 100g and...

Wetlands are ecosystems rich in biodiversity and their ecological importance is recognized worldwide. Sediment samples were subjected to physical-chemical analysis and organic carbon content varied from 3.0% to 4.8%, the clay between 32 and 40%, silt with 41% and 43%, sand coarse varied between 6 and 11% and fine sand between 7 and 16%. The nitroge...

Nisin was encapsulated in silica through sol-gel process by acid-catalyzed routes. The silica xerogels were characterized through nitrogen adsorption isotherms, small-angle X-ray scattering (SAXS), zeta potential, X-ray diffraction (XRD), scanning electron microscopy (SEM), diffuse reflectance spectroscopy (DRS), and Fourier transform infrared spec...

Encapsulation can be a suitable strategy to protect natural antimicrobial substances against some harsh conditions of processing and storage and to provide efficient formulations for antimicrobial delivery. Lipid-based nanostructures, including liposomes, solid lipid nanoparticles (SLNs), and nanostructured lipid nanocarriers (NLCs), are valuable s...

The objective of the present study was to obtain and evaluate the conditions of acid hydrolysis for cellulose nanocrystals (CNC) production, as well as to characterize its chemical and morphological structure and finally to realize and evaluate its application potential on paper as a structural reinforcing agent. From bleached cellulose, CNC is obt...

Background The current relevance of functional foods in human health and well-being has led to an increased interest in developing new types of oral administration systems for probiotics and nutraceuticals. Encapsulation may provide protection and sustained delivery in food formulations. Scope and approach The recent discussions about probiotics e...

Objectives The co-encapsulation of bioactive peptides obtained from degradation of chicken feathers and flexirubin-type pigment produced by Chryseobacterium sp. kr6 into phosphatidylcholine liposomes was investigated. Results Control empty liposomes showed mean diameter of 168.5 nm, varying to 185.4, 102.0 and 98.5 nm after the encapsulation of pe...

The ability of Listeria monocytogenes grow on ready-to-eat food is a major concern in food safety. Natural antimicrobials, such as nisin, can be used to control this pathogen, but the increasing reports of nisin tolerance and resistance make necessary novel approaches to increase its effectiveness, such as encapsulation. The goal of this study was...

In this work, the effect of antimicrobial lipopeptide P34 on Listeria monocytogenes was evaluated for the first time through a proteomics approach. Bacteria were treated with sublethal doses of peptide P34 (F-P34) and P34 encapsulated into nanoliposomes (N-P34), while empty nanoliposomes (NE) and fresh buffer were used as controls. The proteomic an...

A great diversity of biological activities has been attributed to peptides. Despite food proteins are essentially considered as sources of dietary amino acids, bioactive peptides can be released during gastrointestinal transit, food fermentation, food maturation, and through enzymatic bioprocessing in vitro. Specific bioactive peptide databases int...

Recently, renewable and biocompatible polymers have gained increased relevance for food packaging applications. The use of nanostructures for the development of active and intelligent bio-nanocomposite packaging can be beneficial for food safety and quality. Natural polymers, such as chitosan, starch, cellulose and its derivatives, poly(lactic acid...

The antimicrobial peptide produced by Bacillus velesensis P34 has a broad activity against Gram-positive bacteria, showing potential as natural food preservative. In this work, nanocapsules (NCs) containing the peptide P34 were produced using the polymers poly-ε-caprolactone (PCL) or Eudragit RS-100 (EUD), and their antimicrobial activities were as...

Amino acids (AAs) and proteins are major macronutrients in feedstuffs and fish. Due to a paucity of information about tissue-specific AA nutrition in aquatic animals, this review highlights the important energetic roles and functions of AAs (particularly glutamate, glutamine, and aspartate) in some tissues (particularly skeletal muscle, liver, kidn...

The interactions between liposomes and fish myofibrillar protein (surimi ground salted protein, SURP) were evaluated. Liposomes prepared with ultrapure phosphatidylcholine (UPC) or partially purified phosphatidylcholine (PPC) were dispersed at different weight ratio on SURP. Changes in protein stability and structure were evaluated using FTIR, intr...

The antimicrobial activities of Baccharis dracunculifolia DC essential oil (EO) and hydroalcoholic extract (HE) were evaluated. The EO showed broad antimicrobial activity and its synergistic combination with nisin was tested. Major components of EO were nerolidol, beta-pinene and D-limonene, while artepillin C, rutin and cafeic acid were major phen...

Liposomes modified with biomolecules can present better stability and specific structural characteristics, representing a profitable alternative to conventional liposomes. In this study, liposomes formulations containing pectin (PT) or polygalacturonic acid (PA) were prepared by the thin-film hydration method and reverse phase evaporation method fo...

The world demands new solutions and products to be used as dyes for industrial applications. Microbial pigments represent an eco-friendly alternative as they can be produced in large amounts through biotechnological processes and do not present environmental risks, as they are easily decomposable. Moreover, some of these metabolites are recognized...

The elimination of microbial surface contaminants is one of the most important steps in Good Manufacturing Practices in order to maintain food safety. This is usually achieved by detergents and chemical sanitizers, although an increased demand exists for the use of natural products for disinfection purposes. Several natural substances present antib...

Pectinases and other carbohydrate-active enzymes are important for the food industry, mainly for juice processing. In addition, the use of peels to produce enzymes can aggregate value to these agro-industrial residues and at the end of the process enhance qualitatively and quantitatively the juice production. In this work, three different extracts...

This study evaluates the influence of prebiotic carbohydrates, namely fructooligosaccharides (FOS) and galactooligosaccharides (GOS), on the protein expression of Enterococcus durans LAB18S. The strain was cultivated in 10 g L-1 FOS, GOS or glucose (control) and cellular proteins were extracted for mass spectrometry analysis. A total of 771 protein...

Nanostructured zinc oxide (ZnO) prepared by combustion in solution was used to obtain nanocomposites. The ZnO particles were characterized by Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD), and scanning electron microscopy (SEM), showing crystallite size of 32 nm and a superficial area of 32.6 m2 g-1. Nanocomposites with 1, 3, and 5 wt.% of...

The present study aimed to investigate the bioprocessing of wheat middlings with different lactic acid bacteria (LAB) in order to improve biological activities of this by‐product of wheat flour production. The concentration of lactic acid, reducing sugars, and total phenolics, as well as antioxidant, antibrowning, antibacterial and prebiotic activi...

Eudragit RS100 nanoparticles containing Baccharis dracunculifolia DC essential oil (N‐EO) was developed by the nanoprecipitation method. N‐EO presented an average diameter of 151.6 nm, zeta potential (ζ) of +51.7 mV, and encapsulation efficiency of 99.4%. The antibacterial activity of N‐EO and free essential oil (EO) was evaluated in BHI broth agai...

In this work, a comparative analysis of the peripheral cell component (PCC) proteins of Listeria monocytogenes was carried out. The study was conducted on two set of samples consisting of bacteria treated with sub-lethal concentration of nisin and untreated bacteria as control. PCC proteins were extracted by Tris-Urea-EDTA treatment and then subjec...

This study aimed to develop and characterize poly-ε-caprolactone (PCL) nanofibers containing the natural antifungal natamycin by electrospinning technique using four different formulations. Morphological, structural and thermal properties of PCL nanofibers, with or without natamycin, were investigated by scanning electron microscopy, Fourier-transf...

Cyclic lipopeptides (CLPs) from Bacillus strains have demonstrated a wide range of bioactivities making them interesting candidates for different applications in the pharmaceutical, food and biotechnological industries. Genome sequencing, together with phylogenetic analysis of the Bacillus sp. P34, isolated from a freshwater fish gut, showed that t...

Liposomes have been broadly studied as delivery systems for bioactive compounds, although its relatively low stability remains a limitation for commercial application. In this study, phosphatidylcholine (PC) liposomes were prepared entrapping a mixture of garlic extract (GE) and nisin (Nis) using cholesterol (CHO), oleic acid (OA), or octadecylamin...

Halloysite and montmorillonite nanoclays have a wide range of industrial and medical applications, including food packaging. To ensure safe incorporation of these clays into nanocomposites, toxicological screening must be performed. In this study, the acute toxicity of three nanoclays, namely halloysite, bentonite and montmorillonite modified with...

The variation in the bioavailability of oxygen constitutes the environmental conditions found by bacteria in their passage through the host gastro-intestinal tract. Given the importance of oxygen in the defense mechanism of bacteria, it is important to understand how bacteria respond to this stress at a metabolic level. The probiotic strain Enteroc...

Aqueous biphasic systems (ABS) are an interesting alternative for separating industrial enzymes due to easy to scale‐up and low operational cost. The proteases of Pseudomonas sp. M211 were purified through ABS platforms formed by polyethylene glycol (PEG) and citrate buffer salt. Two experimental designs 23 + 4 were performed to evaluate the follow...

Microbial proteases are widely used as commercial enzymes, which have an active role in several industrial processes. The aim of this study was to investigate the production and properties of extracellular proteases from Barrientosiimonas sp. strain V9. The cultivation conditions for protease production were studied using different carbon and nitro...

Carvacrol is a natural antimicrobial capable of inhibiting several microorganisms. The encapsulation of this compound may increase its stability, water solubility and provide controlled release. In this study, carvacrol encapsulated into nanoliposomes (NLC) and polymeric Eudragit® nanocapsules (NCC) was tested against Staphylococcus aureus, Listeri...

The marine environment encompasses a huge biological diversity and can be considered as an underexplored location for prospecting bioactive molecules. In this review, the current state of art about antimicrobial molecules from marine bacteria has been summarized considering the main phylum and sources evolved in a marine environment. Considering th...

The search for yeast with proteolytic activity that can be explored in technology and product innovation was the objective of this study to isolate and identify strains present in liquid whey and evaluate the proteolytic activity of isolates. From the isolated strains were selected for molecular identification, those with proteolytic activity. Veri...

Nanomaterials have been found increasing applications in the food sector. Nanostructured antimicrobials can be incorporated either to food matrix of food packaging or to provide extended safety and quality. However, the interactions and effects of nanomaterials with biological systems are still poorly understood. Nanoparticles can enter the organis...

Phylogenetic analysis and CLPs production in Bacillus P34. Antimicrobial stimulation was effectuated by adding thermally inactivated bacterial cells and fungi spore.

Prebiotic compounds are substrates selectively metabolized by beneficial gut microbiota causing a health-promoting effect. Despite some prebiotic carbohydrates have been largely studied, xylooligosaccharides (XOS) are important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals. This study aimed to investigate the pro...

Streptomycin was encapsulated by sol-gel through acid-catalysed (SA) or base-catalysed (SB) gelation processes and the samples were evaluated for their antimicrobial activity against a spectrum of Gram-positive and Gram-negative bacteria. The xerogels were characterized using a set of techniques including N2 adsorption isotherms, SEM, DLS, XRD, FTI...

The livestock production and subsequent processing of meat results in huge quantities of solid waste such as viscera, bones, skin and keratin-rich materials, including feathers, hair, wool, claws and hooves. In particular, the continuous growth of poultry industry generates massive amounts of feathers as major waste material. The conversion of such...

An in silico genome analysis of the probiotic Bacillus strain FTC01 was performed. The draft genome comprises 3.9 Mb, with a G + C content of 46.6% and a total of 3941 coding sequences. The species of strain FTC01 was defined as B. velezensis during GenBank genome annotation, following the current nomenclature. Eight gene clusters involved in the s...

Salmonella enterica serovar Enteritidis strain SE86 has been associated with several foodborne diseases occurring in Southern Brazil, becoming and important causative agent of human salmonellosis. In this work, the complete genome of the bacterium Salmonella Enteritidis SE86 was sequenced using the Illumina MiSeq platform. An in silico analysis of...

Silver nanoparticles (AgNPs)were synthesized via three routes where silver was reduced by either citric acid (R1), glucose (R2)or glycerol (R3), and were stabilized with (3-aminopropyl)triethoxysilane (APTES). All nanoparticles were encapsulated in silica by the sol-gel process (SiAgE). Structural, textural, morphological and antibacterial properti...

Liposomes have gained great interest in the food and pharmaceutical industry as colloidal carriers of bioactive compounds. In this work, liposomes of phosphatidylcholine (PC) and oleic acid (OA) encapsulating garlic extract (GE) were developed to determine its aptitude as antifungal agent in wheat bread. The influence of GE on the properties of lip...