Adriana Szmidt-Jaworska

Adriana Szmidt-Jaworska
Nicolaus Copernicus University | umk · Chair of Plant Physiology and Biotechnology

PhD, DSc

About

58
Publications
6,376
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448
Citations
Citations since 2016
14 Research Items
296 Citations
2016201720182019202020212022020406080
2016201720182019202020212022020406080
2016201720182019202020212022020406080
2016201720182019202020212022020406080

Publications

Publications (58)
Article
Full-text available
Research interest into the exposure of plants to magnetic fields (MF), including electromagnetic fields (EMF), has increased recently but results often vary depending on factors such as plant species and treatment dose. In this study, we exposed young (one year) and old (four years) field bean (Vicia faba L. (partim)) seeds to EMF (50 Hz, 7 mT) and...
Article
Full-text available
Electromagnetic field (EMF) and its effect on crop plant growth and their quality parameters is increasingly gaining the interest of researchers in agronomic science. However, the exact mechanism of EMF action in plant cells is still unclear. Among the completely unexplored parameters is the relationship between the EMF effects and the seed size. T...
Article
Full-text available
Guanosine 3′,5′-cyclic monophosphate (cGMP) is an important signaling molecule in plants. cGMP and guanylyl cyclases (GCs), enzymes that catalyze the synthesis of cGMP from GTP, are involved in several physiological processes and responses to environmental factors, including pathogen infections. Using in vitro analysis, we demonstrated that recombi...
Article
Full-text available
The plant proteins called ERECTA family play important role in inflorescence architecture, stomatal patterning and phloem-xylem organization. ERECTA proteins belong to the moonlighting proteins family containing the guanylyl cyclase (GC) catalytic center embedded within the intracellular kinase domain. This characteristic architecture of ERECTA pro...
Article
Full-text available
In recent years, cyclic guanosine 3' ,5' -cyclic monophosphate (cGMP) and guanylyl cyclases (GCs), which catalyze the formation of cGMP, were implicated in a growing number of plant processes, including plant growth and development and the responses to various stresses. To identify novel GCs in plants, an amino acid sequence of a catalytic motif wi...
Article
Full-text available
A variety of plant cellular activities are regulated through mechanisms controlling the level of signal molecules, such as cyclic nucleotides (cNMPs, e.g., cyclic adenosine 3 :5-monophosphate, cAMP, and cyclic guanosine 3 :5-monophosphate, cGMP) and calcium ions (Ca2 +). The mechanism regulating cNMP levels affects their synthesis, degradation, eff...
Article
Full-text available
Background: This study describes a promising method for understanding how halophytes adapt to extreme saline conditions and to identify populations with greater resistance. Image and colour analyses have the ability to obtain many image parameters and to discriminate between different aspects in plants, which makes them a suitable tool in combinati...
Article
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RNA extraction involves several main stages, regardless of the method of extraction: homogenization, effective denaturation of proteins from RNA, inactivation of ribonuclease and removal of any DNA, protein, and some residual contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of polysacchari...
Article
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Under the field conditions crop plants interact with diverse microorganisms. These include beneficial (symbiotic) and phytopathogenic microorganisms, which jointly affect growth and productivity of the plants. In last decades, production of potato (Solanum tuberosum L.) suffers from increased incidence of potato virus Y (PVY), which is one of most...
Article
Proteins with a CYTH domain may play a central role at the interface between nucleotide and polyphosphate metabolism. One of the plant CYTH domain-containing proteins from Brachypodium distachyon, BdTTM3, is annotated in NCBI databases as an 'adenylyl cyclase' or a 'triphosphate tunnel metalloenzyme'. The divergent nomenclature and the search for p...
Article
Cell signaling is an evolutionarily conserved mechanism that responds and adapts to various internal and external factors. Generally, a signal is mediated by various signaling molecules and is transferred to a cascade of effector proteins. To date, there is significant evidence that cyclic nucleotides (cNMPs), e.g., adenosine 3',5'-cyclic monophosp...
Article
Full-text available
Efficient integration of various external and internal signals is required to maintain adaptive cellular function. Numerous distinct signal transduction systems have evolved to allow cells to receive these inputs, to translate their codes and, subsequently, to expand and integrate their meanings. Two of these, cyclic AMP and cyclic GMP, together re...
Article
It is generally known that cyclic GMP widespread in prokaryotic and eukaryotic cells, is involved in essential cellular processes and stress signal transduction. However, in contrast to animals the knowledge about plant guanylyl cyclases (GCs) which catalyze the formation of cGMP from GTP is still quite obscure. Recent studies of plant GCs are focu...
Article
Calcium dependent protein kinases (CDPK) are well established plant sensor and effectors for calcium ions and participate in regulation of multiple abiotic and biotic stress responses in plant cells. Here we present the identification and characterization of a new CDPK kinase gene from bulbous plant Hippeastrum x hybr. and examine the role of this...
Article
Full-text available
Phytopathogenic fungi use a variety of strategies to infect hosts and have a diverse sensitivity to the natural defence mechanisms of the host plant, e.g. synthesis of phytoalexins. Many important pathogens of crops belong to the problematic genus Phoma, including species recently reallocated to Peyronellaea. The work presents: (1) molecular identi...
Article
CDPK kinases are a unique class of calcium sensor/responders that regulate many growth and developmental processes as well as stress responses of plants. PnCDPK1 kinase from Pharbitis nil is regulated by light and contributes to seed germination, seedling growth and flower formation. Following an earlier work in which we identified the PnCDPK1 codi...
Article
Cyclic 3',5'-guanosine monophosphate (cGMP) is involved in many physiological processes in plants. Concentration of this second messenger in plant cell is determined by guanylyl cyclases (GCs) responsible for cGMP synthesis and phosphodiesterases (PDEs) involved in cGMP inactivation. First discovered plant GCs were localized in cytosol, but few yea...
Article
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Potato plants can be colonized by various viruses and by symbiotic, saprophytic and pathogenic fungi. However, the significance of interactions of viral infection and fungal colonization is hardly known. This work presents a model experiment in which the influence of three different types of fungal associations on the growth and physiology of the p...
Article
Adenylyl cyclases (ACs) are enzymes that generate cyclic AMP, which is involved in different physiological and developmental processes in a number of organisms. Here, we report the cloning and characterization of a new plant adenylyl cyclases (AC) gene, designated HpAC1, from Hippeastrum x hybridum. This gene encodes a protein of 206 amino acids wi...
Article
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It has been previously suggested that 1-O-IAGluc growth stimulation occurs as the effect of its hydrolysis into a free IAA. In present experiments castanospermine, a known β-glucosidase inhibitor, was included. 1-O-IAGluc in the presence of castanospermine stimulated growth of corn coleoptiles segments even stronger then free IAA. So, it seems that...
Article
Full-text available
Mechanical damage to scales of Hippeastrum x hybr. bulbs leads to the formation of phytoalexin-like compounds which redden the wounded tissue. The reaction is accompanied by an increase in methyl jasmonate (JA-Me). Applying 2-(4-isobutylphenyl) propionic acid, a jasmonate biosynthesis inhibitor, decreases the level of endogenous jasmonates and decr...
Article
The mechanisms by which intra- and extracellular signals induce a specific biological response are important for the regulation of cell function, processes of growth and development and responses to environmental changes. In recent years, enormous progress has been made in studies of elements involved in the regulation of processes occurring in pla...
Article
Signaling pathways, and specifically the signaling pathway of calcium, have been widely implicated in the regulation of a variety of signals in plants. Calcium-dependent protein kinases (CDPKs) are essential sensor-transducers of calcium signaling pathways, the functional characterization of which is of great interest because they play important ro...
Article
Full-text available
The spatial organisation of the splicing system in plant cells containing either reticular (Allium cepa) or chromocentric (Lupinus luteus) nuclei was studied by immunolabelling of SR proteins, snRNA, and the PANA antigen, known markers for interchromatin granule clusters in mammalian cells. Electron microscope results allowed us to determine the di...
Article
Little work was done so far with phytochrome from Pharbitis nil. Purification of phyA from this plant has been exceptionally difficult. Labile phytochrome was presented in too small amount to obtain either absorption spectra or enough protein to produce antibodies. Monoclonal antibodies mAP5, MAC 50, 52, 198 recognized Pharbitis nil labile phytochr...
Article
Full-text available
In this study using biochemical approaches we identified two calcium-dependent protein kinases (CDPKs) named PnCDPK52 and PnCDPK56 in soluble protein extracts from seedlings of Pharbitis nil. Both enzymes phosphorylated the specific substrate histone III-S in the presence of Ca2+ and cross-reacted with antibodies against the CDPK. PnCDPKs exhibited...
Article
Calcium is an ubiquitous, crucial second messenger, that plays an essential and versatile role in cellular signaling. It has been shown to act as an intracellular regulator in many aspects of plant growth, development and stress responses. Many distinct signals induce spatial and temporal Ca(2+) spikes as well as the frequency and amplitude of Ca(2...
Article
Calcium-dependent protein kinases (CDPKs) are central calcium signal decoders. Here we report the isolation and characterization of the Japanese Morning Glory’s (Pharbitis nil) CDPK gene, termed PnCDPK1. The full-length cDNA of 1943bp contains an open reading frame for PnCDPK1 consisting of 514 amino acid residues and a calculated molecular mass of...
Article
It is known that environmental stresses can have devastating effects on plants and represent the most limiting factors for productivity. Apart from biotic stress caused by plant pathogens, there are a number of abiotic stresses such as extremes in temperature, drought, salinity, heavy metals, radiation and mechanical wounding which have detrimental...
Article
Cyclic nucleotides (cAMP and cGMP) play an essential role in many important cellular processes in prokaryotic and eukaryotic organisms. They are produced by purine nucleotide cyclases: adenylyl and guanylyl cyclases. They are classified as one of two distinct forms: soluble and bound to membranes. Beside the differences in enzyme localization, the...
Article
Guanylyl cyclases (GCs) are enzymes involved in the biosynthesis of cyclic guanosine monophosphate (cGMP). Here we report the cloning and characterization of a new guanylyl cyclase, designated PnGC-1, from Pharbitis nil. This gene encodes a protein of 286 amino acids, with a calculated molecular mass of 32kDa. The predicted amino acid sequence cont...
Article
Nitric oxide (NO) is a small gaseous radical molecule previously studied primarily as an air pollutant and metabolic product of certain bacteria. NO's uptake into leaves.. as well as its metabolism and phytotoxicity are well documented. It was subsequently demonstrated that plants not only respond to atmospheric NO but also produce substantial amou...
Article
Cyclic GMP acts as a chemical switch in plant cells to modulate cellular reactions. However, its metabolism has not been extensively explored and is still poorly understood. Previous experiments suggest that an endogenous cGMP system could participate in the mechanism of phytochrome controlled photoperiodic flower induction in Pharbitis nil. In ord...
Article
Full-text available
Light is one of the most important environmental factors influencing the induction of flowering in plants. Light is absorbed by specific photoreceptors--the phytochromes and cryptochromes system--which fulfil a sensory and a regulatory function in the process. The absorption of light by phytochromes initiates a cascade of related biochemical events...
Article
It is known that the level of cGMP is modulated in plant cells in response to a number of stimuli but intracellular events dependent on cGMP metabolism are not clear. Guanylyl cyclases (GCs) are enzymes which are responsible for synthesis of cGMP in eukaryotic and prokaryotic cells. To collect evidence for the participation of cGMP in light signal...
Article
It was revealed that cGMP is involved in the control of photoperiodic flower induction. Further insight into the signalling function of cGMP is likely to be obtained by analysis of its effectors. Therefore, in the present study, we used various agents that cause changes in cGMP-dependent kinase (PKG) activity and examined their effects on the activ...
Article
Cyclic adenosine diphosphate ribose (cADPR) is a potent endogenous calcium-mobilizing agent synthesized from NAD+ by ADP-ribosyl cyclases described for several animal cells. Pharmacological studies suggest that cADPR is an endogenous modulator of Ca2+-induced Ca2+ release channels. There is also information about the sub-micromolar concentration of...
Article
Calcium signals play an important role in many aspects of plant growth and development, including plant response to biotic and abiotic stress. The stimulus characteristic intracellular Ca2+ signals are generated in plant cells by a variety of stimuli, including changes in environmental conditions, interaction with microbes and growth and developmen...
Article
The involvement of cGMP in the regulation of the flowering of Pharbitis nil was investigated through exogenous applications of cGMP and chemicals that are able to change the cGMP level and analyses of endogenous cGMP level. Exogenous applications of cGMP and 8-pCPT-cGMP (a cyclic GMP non hydrolyzed analog) to P. nil plants, which were exposed to a...
Article
Full-text available
A protein, identifiable as calmodulin (CaM), has been isolated from the seedling tissue of Pharbitis nil. The method has been developed to isolate a high quality protein from plant tissue containing the high content of polyphenols. This protein was relatively heat-stable and bound to hydrophobic resin in calcium-dependent manner. It was recognized...
Article
It is known that the level of cGMP is modulated in response to a number of stimuli in plant cells but intracellular events distal to cGMP metabolism are not clear. Cyclic GMP-dependent protein kinase (Pk-G) is a major effector of cGMP action in animals and yeasts. We wanted to determine whether such kinase is present in plant cells. A soluble prote...
Article
Full-text available
A soluble Ca(2+)-dependent protein kinase (CDPK) was isolated from seedlings of the short-day plant Pharbitis nil and purified to homogeneity. Activity of Pharbitis nil CDPK (PnCDPK) was strictly dependent on the presence of Ca(2+) (K(0,5)=4,9 microM). The enzyme was autophosphorylated on serine and threonine residues and phosphorylated a wide dive...
Article
Full-text available
The aim of this work was to determine if there is any relationship between an endogenous phyA level and photoperiodic flower induction. The level of phyA was characterised with polyclonal antibodies directed to phyA from pea. At first it was detected that phyA level is predominant in cotyledons, whereas in roots and stems the concentration of labil...
Article
Full-text available
The exposure of dark‐grown Pharbitis nil seedlings to continuous R induces a rapid decrease in PHYA mRNA abundance with a half‐life of about 2 h. A 5 min R pulse also induces this decline, and the effect is partially reversible by subsequent FR irradiation, confirming that the regulation of expression is mediated via the Pfr form of a phytochrome....
Article
The exposure of dark‐grown Pharbitis nil seedlings to continuous R induces a rapid decrease in PHYA mRNA abundance with a half‐life of about 2 h. A 5 min R pulse also induces this decline, and the effect is partially reversible by subsequent FR irradiation, confirming that the regulation of expression is mediated via the Pfr form of a phytochrome....
Article
A new enzymatic activity, which catalyses formation in vitro of di-O-(indole-3-acetyl)-D-glucose from 1-O-(indole-3-acetyl)-beta-D-glucose has been found in extracts of Zea mays seedlings. The structure of di-O-(indole-3-acetyl)-D-glucose, not as yet described, has been assigned by GC-MS, 1H NMR and ammonolysis.

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Project (1)
Project
This research has as main objective the physiological and molecular control of the induced effect of the magnetic field (MF) in the seeds of a wheat variety of Poland. The promoter effect will be analyzed in the early stages of growth and development of plants, observing how the seeds will react after treatment with MF and hydropriming.