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Adolfo Toledano-Díaz

Adolfo Toledano-Díaz
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria CSIC

PhD

About

151
Publications
28,478
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1,891
Citations
Citations since 2017
62 Research Items
1198 Citations
2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250

Publications

Publications (151)
Article
Full-text available
Introduction and objective Cryopreservation of testicular tissues offers new possibilities to protect endangered species, genetically valuable individuals or even the fertility potential of prepubertal individuals who have died unexpectedly. However, the use of this technique still remains a challenge. In this study, slow freezing and vitrification...
Article
Full-text available
Since the original description of Alzheimer´s disease (AD), research into this condition has mainly focused on assessing the alterations to neurons associated with dementia, and those to the circuits in which they are involved. In most of the studies on human brains and in many models of AD, the glial cells accompanying these neurons undergo concom...
Article
In both captive wildlife and production animals is important to develop strategies for population control. Immunization against GnRH is an easy and inexpensive immunocastration method that reduces the concentration of testosterone and decreases sperm quality. However, its effectiveness depends on the species and repetition of the treatment. This st...
Article
Full-text available
Since Alois Alzheimer described the pathology of Alzheimer's disease in 1907, an increasing number of studies have attempted to discover its causes and possible ways to treat it. For decades, research has focused on neuronal degeneration and the disruption to the neural circuits that occurs during disease progression, undervaluing in some extent th...
Article
Transrectal ultrasonic‐guided massage of the accessory sex glands (TUMASG) is a technique that allows collecting semen requiring few electrical stimuli or even no pulse. A long‐acting analogue of oxytocin (carbetocine, 0.1 mg) was i.v. administered before TUMASG in 10 conscious bucks (Experiment 1) and 10 anesthetized Iberian ibexes (Experiment 2)...
Article
Sperm cryopreservation is the most common procedure used to establish germplasm banks for endangered species - but sometimes sperm cells cannot be obtained. In such cases, freezing testicular tissue may be the only option. The testes contains germ cells at different stages of differentiation, including spermatogonia, primary spermatocytes, secondar...
Article
Aim: This work examined the influence of induced changes in prolactin (PRL) secretion on sperm cryoresistance of ibex and the mouflon. Materials and Methods: PRL secretion was modified in a first experiment by the use of bromocriptine (BCR, dopamine agonist) during the non-breeding season, and in a second experiment by the use of sulpiride (SLP, do...
Article
The expression of aquaglyceroporin 3 (AQP-3) has been demonstrated in the spermatozoa of several mammalian species and its role has been associated with cryotolerance. Post-thaw sperm quality from individual dromedary males with different response to freezing-thawing process was evaluated through sperm head morphometry. In order to understand the c...
Article
Full-text available
The present work aimed to evaluate the chromatin compaction of rooster spermatozoa along the male reproductive tract, and to study the vas deferens lining cells, potentially involved in sperm maturation. Chromomycin A3 (CMA3) was used to determine the chromatin compaction of spermatozoa from testis (T), proximal (including epididymis, V1), intermed...
Article
Full-text available
Background: The Cantabrian capercaillie (Tetrao urogallus cantabricus) is critically endangered. This subspecies has the lowest genetic variability and it is in regression. It belongs to Phasianidae family; therefore, the domestic chicken (Gallus gallus domesticus) could be a good model for developing reproductive technologies for use in capercail...
Article
Full-text available
This work identified the presence of AQPs in frozen-thawed sperm of wild ruminants and assessed the influence of the interaction between photoperiod and thyroxine on AQP expression, and on testosterone secretion. Thyroxine and melatonin were administered to ibexes. In a second experiment, performed in mouflons, circulating thyroxine was reduced via...
Article
Black-footed penguins (Spheniscus demersus) are classified as endangered, and the populations of gentoo penguins (Pygoscelis papua) are rapidly decreasing. The optimization of semen cryopreservation in these species, for preserving their genetic diversity in genome resource banks, is essential for the success of captive breeding programs. This stud...
Article
Sperm vitrification is a simple, inexpensive method that allows the cryopreservation of sperm in the field and for endangered species is a useful alternative to conventional freezing. The study, therefore, is focused on the suitability of vitrification for cryopreserving Iberian wolf sperm and utilizing plasma testosterone concentration as a marker...
Article
1. Birchen and Blue Leonesa are two endangered chicken breeds mainly raised in Curueño Valley in North Spain. The establishment of a germplasm bank to guarantee the preservation of these breeds is needed. However, cockerels from different breeder flocks can show variance in semen cryoresistance. 2. The following work focused on the sperm characteri...
Article
Full-text available
The domestication process has resulted in profound changes in the reproductive physiology of the animals that might have affected the sperm characteristics and thus their sensitivity to handling and cryopreservation procedures. This work assesses the response of the sperm of domestic and wild ungulates to a cooling storage at 15°C for 20 h followed...
Article
Full-text available
In this review, the most important neuropathological changes found in the cerebella of sheep affected by classical natural scrapie are discussed. This disease is the oldest known of a group of unconventional “infections” caused by toxic prions of different origins. Scrapie is currently considered a “transmissible spongiform encephalopathy” (due to...
Article
Full-text available
The roe deer is a monoestrous species with a very short rutting season. The present work reports the most suitable period for collecting epididymal sperm and describes the effect of two cooling rates on the post-thaw quality of sperm. Testes were collected 24–48 h after death. Samples of sperm flushed from the epididymis were subjected to either (1...
Article
Background Recent studies have noted that the circulating testosterone concentration may affect the ability of sperm to survive cryopreservation. However, few attempts to confirm such a relationship have been made. Wild ruminant species have very marked seasonal changes in their reproductive function and strong annual changes in their plasma testos...
Article
Seasonal endocrine changes may modify sperm cryoresistance in certain small ruminant species. The present work examines the effect of prolactin (PRL) on ram and buck sperm cryoresistance. A dopamine agonist (bromocriptine [BCR] 60 mg i.m. twice per week from May 15 to June 15, i.e., approaching the summer solstice) or antagonist (sulpiride [SLP] 10...
Article
Full-text available
The American flamingo is a useful model for the development of successful semen cryopreservation procedures to be applied to threatened related species from the family Phoenicopteridae, and to permit genetic material banking. Current study sought to develop effective sperm cryopreservation protocols through examining the influences of two permeatin...
Article
Full-text available
Aim of study: To examine the influence of administering cyproterone acetate (CPA), at the beginning of the mating season, on the testosterone concentration and morphometric and functional characteristics of ram and buck semen. Area of study: Madrid, Spain Material and methods: Five rams and five bucks were intramuscularly administered 200 mg of CPA...
Poster
Full-text available
INTRODUCCIÓN Uno de los factores que determinará el éxito de la inseminación artificial, como herramienta para difundir comportamientos que favorezcan la actividad cinegética y la conservación de la perdiz roja, es la preservación del semen por refrigeración o congelación. Durante los procesos de almacenamiento espermático se produce un incremento...
Poster
Full-text available
INTRODUCCIÓN Los procesos de maduración de los espermatozoides de gallo a lo largo del tracto reproductivo son poco conocidos. La cromomicina A3 (CMA3) es un fluorocromo que compite con las protaminas para unirse al ADN y se ha utilizado para analizar el empaquetamiento de la cromatina en los espermatozoides de mamíferos (Simes et al., 2009). El ob...
Poster
Full-text available
INTRODUCCIÓN El objetivo del presente trabajo fue evaluar cómo influye el tipo de alojamiento de los gallos (en gallineros con parques exteriores con tierra sin hierba, o con acceso a parques con la típica vegetación mediterránea) en la calidad del esperma, y la respuesta a la criopreservación. Además se estudió la posible correlación entre calidad...
Article
Full-text available
Recent reports showed a positive correlation between frozen-thawed rooster sperm DNA integrity and the concentrations of valine in seminal plasma. The present study evaluated the effect of supplementing valine to semen extender for freezing sperm of 2 endangered local Spanish chicken breeds with different sperm cryoresistance: Red Villafranquina (V...
Article
Full-text available
The domestic ferret (Mustela putorius furo) provides a good model for developing new reproductive technologies for use with threatened related species. Such technologies could also be used in the reproductive management of this pet species. The present work reports an improved freezing protocol for ferret sperm. Semen was collected by electroejacul...
Article
This article describes the urinogenital condition of three female Iberian ibexes (Capra pyrenaica—one infertile 3-yr-old adult and two prepubertal animals aged 1 (PP1) and 2 (PP2) yr, respectively, all raised in captivity. All showed constant urinal dribbling, leading to ulcerative dermatitis in the vulvar area. Housed in a stable with other female...
Article
This study examines the influence of administering testosterone at the end of the mating season, on the responses (morphometric and functional) of ram and buck sperm to freezing-thawing. Five rams were administered 25 mg testosterone propionate (TP) subcutaneously in 2 mL of olive oil twice per week (Monday and Thursday) from October 1 to 31; 5 buc...
Article
Full-text available
Sperm cryopreservation by ultra-rapid cooling based on dropping small volumes of sperm suspension directly into liquid nitrogen, has been successful in some wild ruminant species, including the Iberian ibex (Capra pyrenaica). In ultra-rapid cooling, the contents of these droplets are expected to enter a stable, glass-like state, but to the best of...
Poster
Full-text available
Mito TrackerTM Green [MITO] is a probe capable to stain the mitochondrial membrane of different species; however, we have observed its ability to stain the acrosomal membrane of rooster sperm. The association of MITO and propidium iodide [PI] was used to simultaneously assay the mitochondrial, acrosomal and plasma membranes, of rooster sperm. MITO-...
Article
The Black Crested Mangabey (Lophocebus aterrimus) is an African monkey listed as Near Threatened by the IUCN and in captivity the population is limited to 34 males. The aim of this study was to evaluate two Black Crested Mangabey males, maintained in captivity in a zoological garden and suspected of infertility, with a complete examination of their...
Article
In small ruminants, testosterone and prolactin plasma concentrations show circannual fluctuations as an adaptation mechanism to their seasonal breeding behavior. Sperm resistance to the freezing-thawing process shows seasonal fluctuation throughout the year, with lower sperm freezability at the beginning of the breeding season when prolactin and te...
Article
The aim of this study was to examine ovine sperm cryoresistance during the rutting season (RS) and its association with sperm head area and seminiferous epithelium proliferation. Small ruminants show fluctuating testosterone levels throughout the year, which could interfere with spermatogenesis and sperm cryopreservation. Ejaculates, testicular bio...
Conference Paper
Full-text available
INTRODUCCION El uso de sistemas de alojamiento con acceso a pastos en la producción avícola esta creciendo debido a la demanda de los consumidores de productos más "naturales" (carne y huevos) y procedentes de sistemas respetuosos con el bienestar animal (Sossidou et al., 2011). Elegir correctamente el mejor genotipo para este tipo de sistemas al a...
Poster
Full-text available
La perdiz roja (Alectoris rufa) es una especie de gran importancia ecológica y económica. El deterioro de su hábitat, la intensificación agrícola, el uso de plaguicidas y la introgresión genética con la perdiz chukar (Alectoris chukar) amenazan la conservación de la perdiz roja. La hibridación ha supuesto cambios en el comportamiento y en la biolog...
Article
This study compares the effectiveness of the ultra-rapid and conventional freezing of sperm from captive bovids, giraffids, cervids, ursids, a cercopithecid, a delphinid and a phascolarctid. The relationship between sperm head dimensions and cryosurvival was also examined. Compared to conventional freezing, the ultra-rapid freezing of epididymal sp...
Article
Full-text available
The purpose of this study was to examine the anti-predator behavior in pure red-legged partridges (Alectoris rufa) and hybrids with chukar partridges (A. rufa × (A. rufa × A. chukar)). A total of 117 birds (66 pure and 51 hybrid partridges; 74 females and 43 males) maintained in outdoor cages were used at a range of 52 to 55 wk of age. Four active...
Article
Full-text available
Seminal plasma is a key biological fluid that modulates sperm function in the reproduction process. However, its role in sperm biotechnologies is scarce in poultry. The aims of the present study were to study the amino acids profile and total proteins of seminal plasma in 12 Spanish chicken breeds and to investigate the role of seminal plasma on cr...
Data
Protein concentrations in seminal plasma of 12 Spanish rooster breeds. (PDF)
Data
Motility variables of sperm of 12 Spanish rooster breeds (fresh, frozen with plasma and frozen without plasma). (PDF)
Data
Viability and concentration of fresh sperm. Sperm viability of frozen sperm with plasma and without plasma of 12 Spanish rooster breeds. (PDF)
Data
Amino acids profile in seminal plasma of 12 Spanish rooster breeds. (PDF)
Data
Tunel + of sperm of 12 Spanish rooster breeds (fresh, frozen without plasma and frozen with plasma). (PDF)
Article
The purpose of this study was to examine the anti-predator behavior in pure red-legged partridges (Alectoris rufa) and hybrids with chukar partridges (A. rufa × (A. rufa × A. chukar)). A total of 117 birds (66 pure and 51 hybrid partridges; 74 females and 43 males) maintained in outdoor cages were used at a range of 52 to 55 wk of age. Four active...
Article
In this study, there was an examination of the effect on the characteristics of cryopreserved black-footed (Spheniscus demersus) and gentoo (Pygoscelis papua) penguin semen, of thawing at 37 and 5 °C. For two consecutive years, semen was collected and frozen during the April-June period from six gentoo penguins, and during the October-November peri...
Poster
Full-text available
Birchen Leonesa and Blue Leonesa chicken breeds are highly esteemed for their unique feather, ideal for making flies for trout fishing; unfortunately, both breeds are among the endangered Spanish breeds. Ex-situ cryopreservation of sperm cells in gene-banks is an alternative for conservation of these breeds. However, besides the breed, multiple fac...
Article
Full-text available
Sperm cryopreservation protocols are not well defined in many wild species such as the mouflon. The aim was to study the effect of different concentrations of EY on mouflon epididymal sperm cryosurvival. Samples were collected by the flushing method and cryopreserved by the conventional freezing technique in straws using a TEST (TES, Tris, glucose)...
Conference Paper
Full-text available
Birchen Leonesa and Blue Leonesa chicken breeds are highly esteemed for their unique feather, ideal for making flies for trout fishing; unfortunately, both breeds are among the endangered Spanish breeds. Ex-situ cryopreservation of sperm cells in gene-banks is an alternative for conservation of these breeds. However, besides the breed, multiple fac...
Conference Paper
Full-text available
This study examines the effectiveness of transrectal, ultrasound-guided massage of the accessory sex glands (TUMASG) combined with electroejaculation for obtaining samples in a black-crested mangabey (Lophocebus aterrimus), an African monkey listed as Near Threatened by the IUCN. On February 7 th , this animal was anaes-thetised by a projectile dar...
Article
The aim of the present work was to examine the influence of access to pasture in an outdoor housing system on rooster sperm quality and response to cryopreservation and to examine the possible correlation between values for sperm quality variables and welfare indicators. Two groups of Black-barred An-daluza and Red-barred Vasca roosters were housed...
Article
Full-text available
This study examines the effectiveness of two methods for cryopreserving post-mortem epididymal sperm - conventional slow freezing employing a short equilibration time with glycerol, and ultra-rapid freezing - from the wild ruminant species Ovis musimon (mouflon) and Dama dama (fallow deer). A Tris-citric acid-glucose (TCG) + 12% egg yolk-based medi...
Article
The rate at which lethal intracellular ice forms during sperm cryopreservation is highly dependent on the cooling protocol. The present work compares two cooling protocols for use with Iberian ibex (Capra pyrenaica) sperm by assessing the effects on the motility, viability, and size of frozen-thawed sperm cells. Ejaculates, obtained from six adult...
Article
Full-text available
The fertilizing capacity of pure, fresh avian semen may disappear in just half an hour, hindering its successful use in artificial insemination (AI) projects. Longer storage requires the use of infra-physiological temperatures and of semen diluents that help preserve the spermatozoa but that do not interfere with their fertilizing capacity. This st...
Article
Seminal plasma removal is routine in goat sperm cryopreservation protocols. The classical washing procedure designed to accomplish this usually leaves the pellet resulting from use of this procedure contaminated with dead sperm, debris, and cells other than sperm. This contamination negatively affects viability of sperm after cryopreservation. The...
Article
Background: Recent evidence suggests that obesity, besides being a risk factor for cardiovascular events, also increases the risk of Alzheimer's disease. Insulin resistance is common in all cases of obesity and appears to be the linkage between both diseases. Obesity, often associated with excessive fat and sugar intake, represents a preclinical s...
Article
Full-text available
For many years, the control of reproduction has been considered a brin function, being the hypothalamus the CNS region directly involved. The main neurons described in recent years related to reproduction are the secretory neurons of GnRH, GnIH and Kisspeptine (gonadotropin-releasing hormone; the gonadotropin-inhibitory hormone and by the peptide K...
Article
Aggressiveness is directly related to testosterone concentration, which varies with seasons and in response to female stimulation. The aim was to determine if the frequency and pattern of agonistic interactions between bucks varies seasonally and in response to oestrous female stimulation. In the first study we characterized the pattern of agonisti...
Article
Full-text available
Postcopulatory sexual selection through sperm competition may be an important evolutionary force affecting many reproductive traits, including sperm morphometrics. Environmental factors such as pollutants, pesticides, and climate change may affect different sperm traits, and thus reproduction, in sensitive bird species. Many sperm-handling processe...
Article
Transrectal ultrasonic-guided massage of the accessory sex glands (TUMASG) is an alternative technique for sperm collection which decreases negative animal welfare concerns of electroejaculation. However, quality of collected sperm with TUMASG is less than that collected by electroejaculation. Utilisation of TUMASG may not, however, induce strong m...
Article
The present study reports the effect of shortening the prefreezing equilibration time with glycerol on the quality of frozen-thawed ejaculated sperm from four Mediterranean mountain ungulates: Cantabrian chamois (_Rupicapra pyrenaica_), Iberian ibex (_Capra pyrenaica_), mouflon (_Ovis musimon_) and aoudad (_Ammotragus lervia_). Ejaculated sperm fro...
Article
This work examines the effectiveness of a TCG (Tris, citric acid, glucose, 6% egg yolk and 5% glycerol) and a TEST (TES, Tris, glucose, 6% egg yolk and 5% glycerol) sperm extender in the freezing of mouflon spermatozoa at slow cooling rates, using different pre-freezing equilibration times (2-3 hr). It also examines the tolerance of mouflon spermat...
Article
Full-text available
All members of the families of the neuroglia have the ability to adopt a reactive state ("gliosis" in general, "astrogliosis" "oligodendrogliosis" and "microgliosis" in its specific form) face to situations where importantly is modified homeostasis of the nervous tissue and / or normal functional dynamics of neurons and their accompanying neuroglia...
Article
Full-text available
Obesity is a recognized risk factor for cardiovascular diseases and the main responsible for the resistance to the action of insulin, a state that precedes the development of diabetes type 2. In the last years, researches have demonstrated that there are also common molecular mechanisms between obesity and Alzheimer's disease, to be the more likely...
Article
From birth to death, neurons are dynamically accompanied by neuroglial cells in a very close morphological and functional relationship. Three families have been classically considered within the CNS: astroglia, oligodendroglia and microglia. Many types/subtypes (including NGR2+ cells), with a wide variety of physiological and pathological effects o...
Article
This paper reports the first successful artificial insemination (AI) of red-legged partridges (Alectoris rufa), the fertility rate achieved, and the length of time sperm cells can survive inside the oviduct (i.e., the post-AI fertile period). Semen from 20 mature males was collected by massage and pooled for use in single intravaginal inseminations...
Article
Full-text available
This paper reports the first successful artificial insemination (AI) of red-legged partridges (Alectoris rufa), the fertility rate achieved, and the length of time sperm cells can survive inside the oviduct (i.e., the post-AI fertile period). Semen from 20 mature males was collected by massage and pooled for use in single intravaginal inseminations...
Article
The use of condoms could provide a means of collecting high-quality spermatozoa from different species under physiological ejaculation conditions. However, certain condom materials may affect sperm functionality. This study examined the spermiotoxicity of different commercial condom materials towards ram and goat spermatozoa. Sperm samples were dil...
Article
This work examines the effects of subsequent cycles of freezing-thawing on giant panda (Ailuropoda melanoleuca) sperm morphometry and function, and assesses whether density-gradient centrifugation (DGC) can increase the number of freezing-thawing cycles this sperm can withstand. A sperm sample was collected by electroejaculation from a mature giant...
Article
Computer-assisted systems for the assessment of sperm morphometry (ASMA systems) have been used successfully with several mammalian species. Unfortunately, they have so far been of little use for assessing bird semen, a consequence of the filiform shape of avian spermatozoa. This study compares two staining techniques (Hemacolor(®) and aniline blue...
Article
The objective was to characterize the stress response and the seminal parameters obtained with electroejaculation (EE) or transrectal ultrasound-guided massage of the accessory sex glands (TUMASG) in two captive but nondomestic ruminants, the mouflons and the Iberian ibex under general anesthesia. In mouflons, the physiological responses (heart and...