Aditya PANDHARINATH SarnaikArizona State University | ASU · School for Sustainable Engineering and Built Environment
Aditya PANDHARINATH Sarnaik
Doctor of Philosophy
Looking for faculty positions in the USA and India in Bioengineering, Microbial Biotechnology and Biomanufacturing
About
23
Publications
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Introduction
Aditya PANDHARINATH Sarnaik currently works at Arizona State University, Poly campus. Aditya PS is actively working on large scale cyanobacterial cultivation, harvesting, bioproduction, separation and analysis; along with metabolic and protein engineering of these phototrophs.
Publications
Publications (23)
Alkanes are high‐energy hydrocarbons that are foreseen as next generation biofuels. Cyanobacteria are known to naturally synthesize C15–C19 alkanes; however, the titers are too low to make this a commercially viable process. Therefore, to leverage these photosynthetic platforms for improved alkane production, here we engineered three novel isolates...
Synechococcus elongatus PCC 11801 is a fast‐growing cyanobacterium, exhibiting high tolerance to environmental stresses. We have earlier characterized its genome and analysed its transcriptome and proteome. However, to deploy it as a potential cell factory, it is necessary to expand its synthetic biology toolbox, including promoter elements and rib...
Background
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the most abundant soluble protein in nature. Extensive studies have been conducted for improving its activity in photosynthesis through approaches like protein engineering. Concurrently, multiple biochemical and radiolabeling assays have been developed for determining its activ...
Cyanobacteria are explored as phototrophic cell factories for the conversion of CO2 to chemicals. Although several cyanobacterial platforms have been established, discovering novel cyanobacterial strains with different growth and metabolic traits would be advantageous. Here, we describe de novo genome assembly and pan-genome analysis of eight fast-...
Efficient co‐utilization of mixed sugar feedstocks remains a biomanufacturing challenge, thus motivating ongoing efforts to engineer microbes for improved conversion of glucose−xylose mixtures. This study focuses on enhancing phenylalanine production by engineering Escherichia coli to efficiently co‐utilize glucose and xylose. Flux balance analysis...
Microbial production of esters has recently garnered wide attention, but the current production metrics are low. Evidently, the ester precursors (organic acids and alcohols) can be accumulated at higher titers by microbes like Escherichia coli. Hence, we hypothesized that their ‘direct esterification’ using esterases will be efficient. We engineere...
With multiple applications in food, pharmaceutical, and chemical industries as antioxidant or nonmetabolizable sweetener; the bioproduction of d‐mannitol is gaining global attention, especially with photosynthetic organisms as hosts. Considering the sustainability prospects, the current work encompasses metabolic engineering of a widely used cyanob...
Engineered cyanobacteria are attractive hosts for the phototrophic conversion of CO2 to chemicals. Synechococcus elongatus PCC11801, a novel, fast-growing, and stress-tolerant cyanobacterium, has the potential to be a platform cell factory, and hence, it necessitates the development of a synthetic biology toolbox. Considering the widely followed cy...
In order to improve the potential of cyanobacterial cell factories, Synechococcus sp. PCC7002 was engineered as ‘one cell-two wells bio-refinery’, for ethylene (‘heterologous’ hydrocarbon) and carotenoids (‘natural’ metabolites) production, and demonstrating its outdoor performance. Although the cultures showed better production outdoor, they exper...
Lignocellulosic biomass is an inexpensive and abundant renewable carbon feedstock available for the sustainable production of fuels and chemicals. However, the process for obtaining pentose and hexose sugars from the hemicellulosic components of plant biomass requires the use of expensive purified enzymes. In this study, Bacillus subtilis strains w...
The rising demand for energy to meet the needs for the growing population has instigated the extensive use of fossil fuels. This increasing dependence on fossil fuel consumption is projected to affect the global climate stability, and hence it has become imperative to develop sustainable routes for the generation of clean energy from renewable feed...
Carbon loss in the form of CO2 is an intrinsic and persistent challenge faced during conventional and advanced biofuel production from biomass feedstocks. Current mechanisms for increasing carbon conservation typically require the provision of reduced co-substrates as additional reducing equivalents. This need can be circumvented, however, by explo...
Cyanobacterial research is impeded by the substantial discrepancies between laboratory studies and outdoor performances, despite successful demonstrations of genetically engineered strains for array of compounds. Therefore, evaluation of adaptive responses is necessary to achieve outdoor scale-up cultivation of cyanobacteria. Under current study, c...
Ultra-low temperature (ULT) storage of microbial biomass is routinely practiced in biological laboratories. However, there is very little insight regarding the effects of biomass storage at ULT and the structure of the cell envelope, on cell viability. Eventually, these aspects influence bacterial cell lysis which is one of the critical steps for b...
Microbial systems have been widely studied and exploited through genetic engineering to address industrial needs and societal challenges. However, owing to their complexity, singular approaches often do not yield desired or optimal results, pushing researchers to explore combinatorial strategies. With advances in synthetic biology, various methods...
Cyanobacteria are globally recognized as potential photosynthetic platform cell factories for production of value-added chemicals. Carotenoids are industrially important fine chemicals used in food, pharmaceutical and health-care products. Zeaxanthin has emerged as a high value xanthophyll carotenoid that exhibits superior antioxidant properties ov...
Synechococcus elongatus PCC 7942 has been widely explored as cyanobacterial cell factory through genetic modifications for production of various value-added compounds. However, successful industrial scale-ups have not been reported for the system predominantly due to its obligate photoautotrophic metabolism and use of artificial light in photobiore...
Questions
Question (1)
I am estimating the amount of ethylene in my standard, using headspace sampler coupled with GC-MS, where I am willing to plot a graph by varying ethylene concentration. I am saturating the first vial with std ethylene and then transferring 1ml from the vial into the headspace of the next vial, sort of serial dilution. In the same respect, I am getting highest hit in the NIST library for the first vial, while for the next vial, first hit is Nitrogen (90-95% probability) while succeeding (1-2% probability) hits are for ethylene. Secondly, AUC for both are almost the same. So although the ethylene is reducing in concentration due to serial dilution, AUC remains constant and I cannot use this AUC parameter for getting the standard plot. In such case, whether am I supposed to multiply the AUC by SOME factor, such that ONLY ethylene will be considered or there is some other way to calculate the same like retention index, match/reverse match scores in NIST library?
