Aditya Gupta

• PhD; NPDF
• PostDoc Position at United States Department of Agriculture

Morphologic development of unidentified Sarcocystis sp. schizonts associated with encephalitis in a 9-wk-old gray fox (Urocyon cinereoargenteus) from Texas is described. Sarcocystis schizonts were confined to lesions. They were sparsely distributed and their staining affinity for hematoxylin and eosin varied with developmental stages; immature schi...

Background Bison (Bison bison) and cattle (Bos taurus) are closely related (can interbreed) and they also share many parasites. Cattle are commonly infected with one or more of the eight named Sarcocystis species: Sarcocystis hirsuta, S. cruzi, S. hominis, S. bovifelis, S. heydorni, S. bovini, S. sigmoideus and S. rommeli. Among these, the full lif...

Background Parasites in the apicomplexan genus Sarcocystis infect cattle worldwide. Assessing the economic importance of each such parasite species requires proper diagnosis. Sarcocystiscruzi, a thin-walled species, infects virtually all cattle. The prevalence of the other thin-walled parasite, Sarcocystisheydorni, remains less well established. Th...

During a survey for Sarcocystis infections in Pennsylvania in wild canids, muscles from the tongue and limb were examined microscopically for sarcocysts. Between 9 February 2024 and 11 February 2024, muscle samples were collected from 76 coyotes, 46 gray foxes, and 21 red foxes from Pennsylvania hunter harvested animals. Around 5 g of muscle was ex...

As predators of a wide variety of livestock and wildlife species, wolves (Canis lupus) could serve as definitive hosts for species of Sarcocystis parasites infecting many intermediate hosts. Sarcocystis sporocysts have been observed in the feces of wolves fed infected beef before, but genetic tools have not yet definitively identified which species...

Hepatozoon sp. stages were detected in histological sections of the muscles of 11 of 25 bobcats (Lynx rufus). Parasites were detected from the myocardium of 11, tongues of 4, and limb muscles of 5 of these animals, which had been hunted in Mississippi in 2017. The intensity of infection was highest in the heart. Only asexual stages (meronts) were d...

Trichinella murrelli Pozio and La Rosa, 2000, is the primary sylvatic trichinellid encountered in temperate North America. During a survey for Sarcocystis in wild canids, a single worm matching the morphology of encapsulated Trichinella was observed in a muscle tissue squash from a gray fox male originating from Pennsylvania. The worm was photograp...

Sarcocystis infections were found for the first time in the muscles of 3 of 3 gray wolves (Canis lupus) from Minnesota. Two kinds (thin-walled and thick-walled) of sarcocysts were detected, based on the appearance of the sarcocyst wall. In wolf 1, sarcocysts were thin-walled (<0.5 μm), and without any visible protrusions. Ultrastructurally, the sar...

Muscles of 25 bobcats (Lynx rufus) from remote areas of Mississippi in 2017 were tested for parasites. Testing for Sarcocystis infections included microscopic examination of fresh unstained muscle squashes, pepsin digestion of hearts and tongues, and histological sections of paraffin-embedded tissues. Sarcocystis spp. infections were detected in th...

Rectal contents of 56 adult bobcats (Lynx rufus) in 2014 and 2017 from remote areas of Mississippi were examined microscopically for parasite stages after the sugar flotation method. Among the helminths, eggs/larvae found were: Paragonimus sp. in 12, Toxocara cati-like in 16, trichurid–capillaridlike in 3, hookworms in 27, and lungworms in 28. Amon...

Currently, 7 named Sarcocystis species infect cattle: Sarcocystis hirsuta , S. cruzi , S. hominis , S. bovifelis , S. heydorni , S. bovini and S. rommeli ; other, unnamed species also infect cattle. Of these parasites of cattle, a complete life cycle description is known only for S. cruzi , the most pathogenic species in cattle. The life cycle of S...

Here, we report the first known outbreak of clinical protozoal myeloencephalitis in naturally infected raccoons by the parasite Sarcocystis neurona. The North American opossum (Didelphis virginiana) and the South American opossum (Didelphis albiventris) are its known definitive hosts. Several other animal species are its intermediate or aberrant ho...

Myxozoans are highly specialized metazoan parasites with a very diverse host range that live on aquatic hosts. In both wild and farm-raised fish, myxozoans are the most frequent parasites. The aim of the current study was to evaluate any myxozoan parasite found in different organs of Schizothorax richardsonii. Live fish were transported with artifi...

Mullets (Mugilidae) are economically important fish in Israel. Two species of mugilids (i.e., the thinlip mullet Chelon ramada and the flathead grey mullet Mugil cephalus) have been stocked in the Sea of Galilee (Lake Kinneret) in order to increase fishermen’s income and lake water quality. These catadromous species do not reproduce in the lake, co...

I presented a poster describing two species infecting Chelon ramada from Sea of Galilee, Israel

During the present study, we describe a novel myxozoan that forms plasmodia in the gill filament of Schizothorax esocinus collected from River Jhelum of Kashmir Himalayan region (n = 26). Description of the new species is based on morphology of the myxospore and on partial 18S rDNA gene. The results showed that the plasmodia were large, visible wit...

Out of the total 2600 myxozoan species reported so far, Thelohanellus Kudo, 1933 is the second most prevalent genus comprising of about 150 species after Myxobolus Butschli, 1882 infecting both freshwater and marine fishes. In general, these are mostly histozoic (within the tissues) and sometimes coelozoic (in body cavities). The present paper deal...

Two novel myxosporeans have been identified during the present study namely, Myxobolus upmae sp. nov. infecting gills of Hypophthalmichthys nobilis and Myxobolus hardevi sp. nov. infecting gills of Labeo bata inhabiting Ranjit Sagar Wetland, Punjab (India). The identification was done on the basis of morphological and molecular attributes of the my...

Beekeeping is the maintenance of honey bee colonies, commonly in hives, by humans. It is the rearing of bees in captivity for obtaining honey, bee wax and for artificial pollination. Honey is a natural sweet substance produced by honey bees, from the nectars of plant flowers and honey dew (White, 1969). The present study aim to analysis the physiol...

The present study deals with the morphological and molecular characterization of an amphistomid trematode, Paramphistomum epiclitum Fischoeder (1904) collected from Capra hircus (goat) in Chandigarh using Gower’s carmine stain and investigating nucleotide sequences of 28S ribosomal RNA and internal transcribed spacer 2 (ITS2) gene markers. A total...

The present study deals with the morphological and molecular characterization of an anoplocephaline cestode Moniezia sp. collected from Capra hircus in Chandigarh using Gower’s carmine stain and investigating nucleotide sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) and internal transcribed spacer 1 (ITS1). Total of 10 gut collect...

A new myxobolid, Thelohanellus gabori (Myxosporea: Myxozoa) parasitizing gill filaments of Crossocheilus latius (Hamilton, 1822) has been described using light microscopy, histopathology, and molecular analyses. A total of 80 live specimens of C. latius were examined, revealing a prevalence of infection of 25.66% (21 of 80 fish sampled). The plasmo...

The sequences of 18S and 28S rDNA have been extensively used as molecular markers to resolve phylogenetic relationships among various myxosporeans infecting fishes. Two novel myxosporeans have been identified during the present study namely, Henneguya latiusii sp. nov. infecting gills of Crossocheilus latius and Myxobolus atkinsoni sp. nov. infecti...

Morphological and molecular characterization of the amphistomid trematode, Gastrothylax crumenifer (Creplin, 1847) Poirier 1883 collected from Capra hircus goat in Chandigarh was carried out using Gower's carmine stain and by investigations on the nucleotide sequences of 28S and internal transcribed spacer 2 (ITS2). A total of 10 guts of C. hircus...

A new myxobolid, Thelohanellus pathankotensis parasitizing gill lamellae and caudal fins of the Indian minor carp Labeo dero has been described using light microscopy, histopathology and molecular analyses. The fish host was collected from a cold water wetland, Ranjit Sagar Wetland, Punjab, India located 32 ̊ 26 ̍ 30 ̎ N Latitude and 75 ̊ 43 ̍ 30 ̎...

In a study conducted from July, 2014 to August, 2015 on the myxosporean infections in freshwater fishes of Ranjit Sagar Wetland (Punjab), many species belonging to the genus Myxobolus were recorded. In this paper, new host, new site of infection and new locality of two myxobolid species viz., Myxobolus saugati Kaur and Singh, 2011 and M. stomum Ali...

In a study conducted from July, 2014 to August, 2015 on the myxosporean infections in freshwater fishes of Ranjit Sagar Wetland (Punjab), many species belonging to the genus Myxobolus were recorded. In this paper, new host, new site of infection and new locality of two myxobolid species viz., Myxobolus saugati Kaur and Singh, 2011 and M. stomum Ali...

The Indian major carp, Labeo rohita Hamilton, 1822 is a tropical freshwater cyprinid fish native to inland waters in Asia. Herein, we describe a novel myxozoan forming plasmodia in the gill lamellae of L. rohita from Ranjit Sagar Wetland in Punjab, India. Myxospores were consistent with the genus Myxobolus, round to elliptical in valvular view, lem...

The myxosporean genus Thelohanellus Kudo, 1933 (Myxozoa: Myxosporea: Bivalvulida) comprises a total of 108 nominal species and is the second largest genus after Myxobolus. They are characterized by the presence of single polar capsule in the myxospore body. The species such as T. filli, T. neocyprini sp. nov, T. theinensis sp. nov., T. dykovae sp....

A myxozoan parasite belonging to the genus Thelohanellus Kudo, 1933 (Cnidaria, Myxosporea, Bivalvulida) was isolated from the fins of Labeo dero inhabiting Ranjit Sagar Wetland, Punjab, India. The plasmodium was 0.5-0.7 mm in diameter each containing 80-100 number of myxospores. Myxospores were egg shaped to ovoidal in valvular view having bluntly...

In the present study, a new species, Thelohanellus muscularis n. sp. infecting head muscles of Labeo rohita (Family Cyprinidae) from Ranjit Sagar Wetland, Punjab, India has been described on the basis of its morphology, histopathology and molecular analyses. The plasmodia were visible with the naked eye in the head muscles (opercular region) as rou...

In the present study, a new species, Thelohanellus muscularis n. sp. infecting head muscles of Labeo rohita (Family Cyprinidae) from Ranjit Sagar Wetland, Punjab, India has been described on the basis of its morphology, histopathology and molecular analyses. The plasmodia were visible with the naked eye in the head muscles (opercular region) as rou...

The pool barb, Puntius sophore Hamilton, 1822, is a tropical freshwater cyprinid fish native to inland waters in Asia. Herein, a novel myxozoan from plasmodia on the caudal fin of P. sophore from Ranjit Sagar Wetland in Punjab, India is described. Myxospores were consistent with the genus Myxobolus, ellipsoidal in frontal view, lemon-shaped in late...

The present study deals with a noval myxosporean described on molecular and histopathological basis from a cold water wetland in Punjab by studying 18S and 28S rRNA gene

A myxozoan parasite belonging to the genus Thelohanellus Kudo, 1933 (Myxosporea, Bivalvulida) was isolated from the gill lamellae of Labeo bata (Hamilton, 1822) inhabiting Ranjit Sagar Wetland in Northern parts of Punjab, India. Histopathology of the infected gills indicated lamellar distortion due to the presence of plasmodia. The intensity of inf...

A synopsis of 52 nominal species of the genus Thelohanellus Kudo, 1933 reported to infect freshwater fishes in India is presented. These species infect various organs such as gills, fins, scales, skin, muscles, gall bladder, kidney etc. The diagnostic features of the genus include tear-shaped myxospores with smooth shell valves and single polar cap...

The present study was aimed to isolate myxozoan parasites infecting the freshwater fish Labeo rohita from Ranjit Sagar Wetland, Punjab from September, 2014 to August, 2015. Out of 50 fish specimens examined, 10 were found to be infected. The identification upto species level was done on the basis of morphological and morphometric attributes of the...

Myxozoans are an economically important group of microscopic metazoan parasites of fish. The myxozoan species Thelohanellus filliinfects commercially important freshwater fish both in wild and in cultured habitat. This parasite causes gill hemorrhagic disease and significant damage to the respiratory surface of the infected fish. In the present stu...