Adil Hussain

Adil Hussain
Pakistan Council of Scientific and Industrial Research | PCSIR · FBRC

Ph.D Biotechnology

About

34
Publications
21,040
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
156
Citations
Citations since 2017
29 Research Items
155 Citations
20172018201920202021202220230204060
20172018201920202021202220230204060
20172018201920202021202220230204060
20172018201920202021202220230204060
Introduction
Dr. Hussain currently works at Pakistan Council of Scientific and Industrial Research (PCSIR). He does research in molecular biology, natural products chemistry with biological activities, biotechnology, microbiology, systematics and taxonomy using morphological, anatomical, palynological and molecular data with phylogenetics approach.
Additional affiliations
August 2015 - February 2016
International Islamic University, Islamabad
Position
  • Lecturer
January 2015 - June 2019
International Islamic University, Islamabad
Position
  • Researcher
September 2014 - February 2019
International Islamic University, Islamabad
Position
  • Professor (Assistant)
Education
September 2014 - August 2017
September 2012 - August 2014
July 2007 - March 2011
Karakoram International University
Field of study
  • Agriculture and Food Technology

Publications

Publications (34)
Article
Full-text available
Recently, most researches have focused on the biological activities of the extracts obtained from different Artemisia species due to the presence of essential compounds with strong activity against some gram-negative and gram-positive bacteria. In this study, five extracts of Artemisia rutifolia Stephan ex Spreng. from the northeastern Gilgit-Balti...
Article
Introduction Different classes of disease-causing viruses are widely distributed universally. Plant-based medicines are anticipated to be effective cures for viral diseases including the COVID-19, instigated by severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). This study displays the phylogenetic perspective of reported species of...
Article
Full-text available
Diversity in eleven Artemisia species from northern Pakistan was assessed based on as per suitability of their elemental contents with thermal conductivity detection and ICP-AES procedures. Results indicated the presence of 13 major elements in the Artemisia species with varied concentrations including Carbon (45.7%, 45,7000 ppm–49.8%, 49,8000 ppm)...
Article
Full-text available
Background-Gilgit-Baltistan, the Northeast region of Pakistan, is well known for its floristic diversity, including members of the genus Artemisia. Artemisia is a large, taxonomically complex genus including ~500 species of both herbs and shrubs. This study was conducted to determine the phylogenetic position of ten undescribed Artemisia taxa from...
Article
Full-text available
Background: Cannabis has a very extensive history of its uses as a medicinal plant that likely dates back more than two millennia. This review was envisioned to provide a brief summary on ethnobotany, phytochemistry, medicinal uses and some biological activities of Cannabis (hemp) with emphasis on its legalization and regulation in Pakistan. Metho...
Article
BACKGROUND: Sea buckthorn (SBT) (Hippophae rhamnoides L.), a shrub rich with a huge variety of vitamins, antioxidants and other biologically active constituents with pharmacological and cosmaceutical applications. OBJECTIVE: The current review aims to compile the recent reported data on the ethnobotany, phytochemistry, biological activities and cos...
Article
This study was carried out to have a closer investigation on taxonomic position of the northeastern Pakistani Ajania fru- ticulosa with 3 genera viz., Artemisia, Ajania and Chrysanthemum. To achieve this, the nrDNA-ITS (nuclear ribosomal internal transcribed spacer) region was newly sequenced for A. fruticulosa (strain PMNH-041623) and pooled with...
Preprint
Full-text available
Abstract Background: Non-synchronized pods shattering in the Brassicaceae family bring upon huge yield losses around the world. The shattering process was validated to be controlled by eight genes in Arabidopsis, including SHP1, SHP2, FUL, IND, ALC, NAC, RPL, and PG. We performed genome-wide identification, characterization, and expression analysis...
Article
Full-text available
Background Non-synchronized pods shattering in the Brassicaceae family bring upon huge yield losses around the world. The shattering process was validated to be controlled by eight genes in Arabidopsis , including SHP1 , SHP2 , FUL , IND , ALC , NAC , RPL , and PG. We performed genome-wide identification, characterization, and expression analysis o...
Article
Full-text available
This research study aimed to estimate the total aflatoxins in corn used for livestock feed intended for breeding cattle, beef, and mature poultry. Total 60 corn samples for livestock feed were collected from different markets and feed shops in Lahore. Total aflatoxin AFB1, AFB2, AFG1, and AFG2 were estimated using Thin Layer Chromatography (TLC) an...
Article
Full-text available
Artemisia chinensis L. referred as Crossostephium chinensis (L.) Makino in the Flora of China is a rare and conceivably threatened plant species with an unclear origin in Asia. The species has been acknowledged so far from some islands of Taiwan. However, as it is extensively cultivated for ornamental and medicinal purposes in Japan, China, and the...
Article
Full-text available
Citation: Akhtar, N.; Hafiz, I.A.; Hayat, M.Q.; Potter, D.; Abbasi, N.A.; Habib, U.; Hussain, A.; Hafeez, H.; Bashir, M.A.; Malik, S.I. ISSR-Based Genetic Diversity Assessment of Genus Jasminum L. (Oleaceae) from Pakistan. Plants 2021, 10, 1270.
Article
α-amylase is the key digestive enzyme that has been used widely in food, paper, detergent and textile industries for starch degradation. This study was conducted for the optimization and characterization of α-amylase production from Aspergillus niger SAIB-4. The study further assessed the effect of metal nanowires (NWs) on starch hydrolysis by α-am...
Article
Full-text available
Lipase is an important commercial enzyme with unique and versatile biotechnological applications. This study was conducted to biosynthesize and characterizes alkaliphilic lipase by Exiguobacterium sp. strain AMBL-20T isolated from the glacial water samples of the northeastern (Gilgit-Baltistan) region of Pakistan. The isolated bacterium was identif...
Article
Jasminum L. is the largest genus containing ~200 species found wild mostly in the tropical regions of the world. The comparative palynological study of nine Pakistani Jasminum species with SEM showed zonocolpus, trilobate, and tricolpus pollen types with simple endocolpus apertures which are plesiomorphic and conserved in the Jasminum species. The...
Article
Full-text available
The present study reports some important species of the genus Artemisia with their folk medicinal uses from the Northeastern (Gilgit-Baltistan) region of Pakistan. The localities where the awareness of traditional information was not reported previously were surveyed and the local traditional knowledge about novel species of Artemisia was reported....
Article
Full-text available
Artemisia is the largest genus in the tribe Anthemideae having ecologically, morphologically, and chemically diverse species. These species are found mostly in the Northern hemisphere, with the distribution of fewer taxa in the Southern hemisphere of the world. Species of this genus have great therapeutic potential and constitute a remarkable natur...
Article
Full-text available
Artemisia chamaemelifolia Vill. is a perennial herb from the genus Artemisia of the Asteraceae family. This plant is mostly distributed in Armenia, Russia, Georgia and the Eurasian mountains including Cantabrian chain, Spanish Sierra Nevada, the Alps (France and Italy), Azerbaijan, Pyrenees, the Balkan range (Bulgaria), northeast and east Anatolia...
Article
Full-text available
Background: There exist natural antioxidants in plants that scavenge harmful free radicals from the body. Free radicals are species of chemical origin with an unpaired electron and play a pivotal role in combating against health-related problems like lung damage, inflammation, and cardiovascular ailments etc. Antioxidants halt the development of th...
Article
Full-text available
Artemisia verlotiorum Lamotte (Asteraceae) is a species of Artemisia described from Europe but native to East Asia. It is an alien and/or invasive species that has become naturalized in many European regions, Australia, South America, New Zealand, North and South Africa, and Western Asia. In continuation of our work on Northeastern Pakistani Artemi...
Article
Full-text available
This study was accomplished to scrutinize the pollen morphology of 15 species of the genus Artemisia of the family Asteraceae from Gilgit-Baltistan region of Pakistan by means of scanning electron microscopy (SEM). Results revealed pollen grains of Artemisia species with tricolporate shape, and characterized by globular symmetry (ellipsoid ball sha...
Article
Full-text available
The primary long-lasting objective of biomedical researcher’s is the expansion of some superior and fruitful conducts in order to make some targeted alterations to the living cells genome. In modern times, a novel and up-to-date tool have emerged which is CRISPR-associated protein-9 nuclease (Cas9) from a bacteria Streptococcus pyogenes. This tool...
Article
Full-text available
e study was performed to isolate cold active alkaline protease and alkaline active lipase producing psychrotrophic bacteria from water and soil samples collected from di erent glaciers of Karakorum Range of mountains, Pakistan. Serial dilution and plating approaches were exploited for bacterial isolation and the isolates were qualitatively screened...
Article
Full-text available
This study for the first time examined foliar epidermal anatomical characteristics (Epidermal cells, types of stomata and trichomes) of thirteen Artemisia species (Including rare ones) with Light microscopy (LM) and Scanning electron microscopy (SEM) from the Northeast (Gilgit-Baltistan) region of Pakistan. The epidermal cells varied from polygonal...
Article
Full-text available
Bacteria produced maximum thermolabile alkaline lipase at 25°C after 96 h incubation in alkaline conditions. We tested the effect of different salts on its activity and found that CaCl 2 stimulated activity by 3766 U, whereas, the ZnSO 4 and FeCl 3 strongly inhibited the activity. A novel organic solvent stable alkaliphilic lipase enzyme (GenBank:...
Article
Full-text available
Salt stress is the important abiotic stresses depressing gainful crop production. This study assessed rice morphologically with DREB1A gene under inducible and constitutive promoters against salinity. Non transgenic and transgenic rice plants with two transgenic events (35S-DREB1A and LIP-9 DREB1A) were assessed under salt stress and controlled con...
Article
Full-text available
A huge amount of scientific literature is available about plant extracts and their compounds having great pharmacological importance. Although, plant-based medicines have been used since antiquity, but knowledge about their effectiveness on human health is still unclear. Artemisia genus belongs to the plant family Asteraceae. This genus comprises o...
Article
Full-text available
This study was undertaken with the aim of analysing some morphological parameters, screening of important phytochemical compounds and the activity of Aloe vera leaf extracts against two fungal species viz; Aspergillus niger and Rhizopus. Morphological assessment of A. vera plant showed significant differences in leaf and root attributes including i...
Article
Full-text available
Solanum nigrum L. (black nightshade) is a wild vegetable with numerous ethnomedicinal potentials. In this study phytochemical analysis and antibacterial activity of fruit, leaf and stem extracts of Solanum nigrum L. prepared in four solvents i.e., ethanol, chloroform, petroleum ether and distilled water were inspected. Phytochemical examination sho...
Article
Full-text available
The necessities of storage are very crucial aspects for better quality and shelf life of fruit pulp both at industrial and household level. In this study a locally grown Apricot variety (Halman) was selected and its pulp was given with chemicals treatment (Sodium Benzoate and Potassium Metabisulphite) in comparison with control samples and their va...
Article
Full-text available
30 Samples of five poultry meat products including chicken nuggets (S1), chicken fillets (S2), chicken burgers (S3), chicken meatballs (S4) and chicken kababs (S5) were collected from various retailers from Hyderabad market to evaluate quality and safety parameters. All the samples were investigated for pH, water holding capacity (WHC), moisture, a...
Article
Full-text available
Apricot pulp is a perishable product and the pulp has a short shelf life (3-5 days) at ambient temperature, and hardly 2-4 weeks at cold storage. Hence, the preservation of the pulp for quality retention and to extend the storage shelf life is important consideration. In this work Storage stability of Apricot pulp (Ap) was determined at room temper...
Article
Full-text available
Background: There exist different methods to preserve the nutrition, color and taste of the fruit pulp for prolonged availability. Bacterial and fungal growth greatly affect the texture and taste of the pulp, if stored for longer period of time. Evaluation of different chemical preservatives to check efficacy and effects on microbial culture growth...

Network

Cited By

Projects

Projects (2)
Archived project
The emergence and spread of antibiotic resistance, as well as the evolution of new strains of disease causing bacterial and fungal agents, are of great concern to the global health community. Artemisia species are important due to having antimalarial properties, extensive pharmacological and ethno botanical applications and the significance of their bioactive compounds like Artemisinin. Commonly used medicinal plants of our community especially Artemisia plants could be an excellent source of drugs to fight off this problem. Moreover, high concentration of elements present in plants can have adverse effect on plant growth and human beings. The elevated level of these elements exceeding the normal concentration levels as recommended by the International safety standards for all medicinal plants is a serious threat to human and animals. The specific goals of this project are: Screening of organic solvent extracts of selected Northeastern (Gilgit-Baltistan) Pakistani Artemisia extracts for the potential antimicrobial activity against phyto-pathogens and clinically important standard reference bacterial and fungal strains Assessment of elemental content in selected Northeastern (Gilgit-Baltistan) Pakistani Artemisia species due to their extensive pharmacological and ethno botanical practices and the significance of their bioactive compounds.
Archived project
In Pakistan, currently 38 species of the genus Artemisia (Asteraceae) have been identified so far. These Artemisia species were found in different phytogeographical regions including the Gilgit-Baltistan region of Pakistan. In this doctoral thesis, emphasis have been given to the ethnobotany, floral morphology, foliar epidermal anatomy (Epidermal cells/stomata types and diversity in trichomes), pollen morphology, phytogeography and molecular phylogeny of Artemisia species from Gilgit-Baltistan region of Pakistan. This study explored some rare species of the genus Artemisia and their folk medicinal uses from Gilgit-Baltistan region of Pakistan. The areas with deprived documentation of traditional information were covered and the native traditional medicinal awareness of the Artemisia species was acknowledged. For the first time in this investigation, 15 Artemisia species were explored and presented in the form of plates. These ethnobotanicaly important species were, Artemisia absinthium L., Artemisia annua L., Artemisia austriaca Jacq., Artemisia biennis Willd., Artemisia campestris L., Artemisia chamaemelifolia Vill., Artemisia herba-alba Asso., Artemisia indica Willd., Artemisia maritima L. Ex Hook. F., Artemisia rutifolia sub sp., Artemisia rutifolia var., Artemisia scoparia Waldst. & Kit., Artemisia sieversiana Ehrhl. Ex Willd., Artemisia verlotiorum Lamotte., and Artemisia vulgaris L. The native people of different regions of Gilgi-Baltistan uses different parts of these Artemisia species as food, ornaments, fuel and for medicinal purposes and were employed against more than 30 different kinds of ailments. The morphology of this genus is very complex and difficult to address. This is because the same species shows different forms under certain ecological conditions. Therefore, it was a dire need to revise the morphology of Artemisia species from Gilgit-Baltistan region of Pakistan. In this study, 66 morphological characters of 20 Artemisia species were nominated for the cladistics analysis of genus Artemisia. The consequential cladogram divided genus Artemisia into five major clades. Subgenus Artemisia and subgenus Absinthium were dispersed between the clades of other sections and appeared as a polyphyletic. All the remaining sections were found to be monophyletic. Subgenus Seriphidium was grouped with Artemisia clade which authenticates its recombination with the genus Artemisia. The micro-morphological attributes of foliar epidermal cells of 19 Artemisia species and stomata types of 17 Artemisia species using SEM and LM were evaluated. Primary objective was to assess the diversity in epidermal cells of these important species for taxonomists to ensure their identification and delimitation. This study disclosed taxonomically essential epidermal attributes of some rare Artemisia species from Gilgit-Baltistan region of Pakistan. Epidermal cells investigated were varied from polygonal to irregular and elongate in shape, while wavy to smooth in margins. Investigation of stomata revealed five different types namely, anomocytic, diacytic, anomotetracytic, anisocytic, and paracytic, which were unequally distributed on both the abaxial and adaxial sides of studied Artemisia species. The diversity in foliar trichomes of 17 Artemisia species obtained from Gilgit-Baltistan area of Pakistan were assessed and presented in the form of plates. A total of 10 main types of trichomes (Glandular and non-glandular) were noticed using light and scanning electron microscopic observations. The 4 glandular trichomes observed were peltate, pluricellular, capitate and thin necked trichomes, while the 6 non-glandular trichomes noticed were aduncate, unicellular calavate, conical type, stinging hair type, unicellular tector and unicellurar filiform. The pollen morphology of 22 species of genus Artemisia from Gilgit-Baltistan region of Pakistan was assessed by means of scanning electron microscopy (SEM). This inquiry revealed pollen grains of Artemisia with tricolporate shape, characterized by globular symmetry (ellipsoid ball shaped from equatorial side and three lobed rounds from polar view) with few exceptions. Additionally, the pollens were marked with reduced spinules on their surfaces which are unique investigative character for the genus Artemisia of Asteraceae family. In this study, seven micromorphological characters (Pollen type, pollen shape, arrangement of spinules, exine sculpture, spinules base, equatorial width and polar length) of pollen grains of different Artemisia species were documented. By using light microscopy and scanning electron microscopy, these 7 characters of Artemisia pollen were nominated for the cladistics and cluster analysis using PHYLIP and MVSP softwares. A data matrix was generated using these micro morphological traits of pollen for cladistics and cluster analysis. The pollen traits are quite indicative for studies concerning the evolutionary kinships of species. The cladistic analysis based on the micromorphological characters of pollen confirmed the reunion of subgenus Seriphidium with Artemisia. In the subsequent cluster analysis, 5 groups within the genus Artemisia have been documented. The investigations based on phytogeography of Artemisia as per collection data of this study confirmed the occurrence of majority of (44 %) Artemisia species in Gilgit district as compared to other districts of the region. This study substantiated that A. maritima and A. sieversiana are the most commonly found species in all parts of the studied districts. Moreover, A. maritima and A. herba-alba are most dominant in the hills and mountains of different districts of Gilgit-Baltistan region of Pakistan. For the molecular phylogeny, the internal transcribed spacer (ITS), external transcribed spacer (ETS) of nuclear ribosomal DNA (nrDNA) and intergenic spacer (psbA-trnH) of choloroplast DNA (cpDNA) sequences of 28 Artemisia species collected from Gilgit-Baltistan region of Pakistan were utilized. The analyses have been done with maximum-likelihood (ML), maximum-parsimony (MP), neighbor joining (NJ) and Mr. Bayes algorithms. The results of this study confirmed the polyphyletic nature of subgenus Artemisia and Absinthium. Other subgenera like Seriphidium, Tridentata, Pacifica and Dracunculus were found to be monophyletic. In this study, 10 undescribed taxa of Artemisia have also been observed. On the basis of phylogenetic results, these taxa were categorized in to different groups (Group I, II & III). The undescribed taxa with in group I was represented by one taxon (A. sp.-AD-H), whereas 4 undescribed taxa (A. sp.-A, A. sp.-B, A. sp.-C and A. sp.-E) were included in group II. Five undescribed taxa (A. sp.-D, A. sp.-F, A. sp.-G, A. sp.-H and A. sp.-I) were placed in group III. In all of them, 1 undescribed taxon within group I was placed in the subgenus Dracunculus with Artemisia japonica and Artemisia desertorum. Four undescribed taxa within group II were placed in the subgenus Absinthium with Artemisia rutifolia and the clade was designated as A. rutifolia complex. Five undescribed taxa within group III were also placed in the subgenus Absinthium with Artemisia sieversiana. Based on the current data and all available in literature, it is concluded that the morphological studies coupled with modern molecular techniques may lead to the clear infrageneric classification of the genus Artemisia. It will also clarify and characterize the undescribed taxa reported in this study.