Adel Sharaf Al-Zubairi

Albaha University · Department of Laboratory Medicine

Background and Objective: Herbal medicinal plants are widely used in Al-Baha region, Saudi Arabia either in rural or urban areas for the treatment of several diseases. Here we investigated the phytochemical and biological effects of three herbal plant extracts, Aloe vera, Jatropha and Ricinus communis. Methodology: We have investigated the total ph...

Zerumbone (ZER) is a cytotoxic phytochemical component extracted from the tropical herbal plant, Zingiber Zerumbet smith. It has been identified as an active anticancer compound. This study was aimed to evaluate in vitro the anti-proliferative activity of this bioactive compound, zerumbone, on seven cancer cell lines namely: CEMss (T4-lymphoplastoi...

Objective: Several natural products are being increasingly used in the treatment of cancer to minimize the adverse side effects of cancer chemotherapy. Zerumbone (ZER), the sesquiterpene derived from Zingiber zerumbet Smith, has been reported to have an in vitro anticancer effects against various human tumour cells as well as in vivo against a numb...

Several natural products are being increasingly used in treatment of cancer to minimize the adverse side effects of cancer chemotherapy. Zerumbone (ZER), the sesquiterpene derived from Zingiber zerumbet smith, has been reported to have an in vitro anticancer effects against various human tumour cells as well as in vivo against a number of induced m...

Essential Oils (EOs) are used in many products to be intended for human utilization. Despite their pharmacological applications in the folk and traditional medicine, studies on EO anti-proliferative properties are still limited. The aim of this study was to investigate the anti-proliferative properties of hydrodistilled EO from 16 aromatic plants g...

Plant's natural extracts such as Essential Oil, are being increasingly used as preservatives as well as in many human used products. The antimicrobial and antioxidant properties are essential for the use of such products, and therefore, their comparison with synthesized preservatives is the basis to replace the latter and for better biological acti...

Due to its stimulating and pleasurable effects, chewing the leaves of khat (Catha edulis) is a widespread habit among the people of East Africa and Arabian Peninsula. The aim of this study was to assess the genotoxicity of the fresh khat leaves extract in CHO cell lines using two cytogenetic endpoints, Chromosomal aberrations (CAs) assay and Micron...

Objective: Frequency patterns of the lactase persistence (LP)-associated -13,915 G allele and archaeological records pointing to substantial role played by southern regions in the peopling and domestication processes that involved the Arabian Peninsula suggest that Southern Arabia plausibly represented the center of diffusion of such adaptive vari...

Background: Catha edulis (Khat) is an evergreen shrub or small tree, traditionally used by various peoples of the Arabian Peninsula and Africa as an integral component of the socioeconomic traditions. It is believed that the psychostimulant nature and toxic nature of khat is primarily due to the presence of cathinone and cathine respectively. Stud...

Background and Objective: Zerumbone (ZER) is a sesquiterpene isolated from the rhizomes of Zingiber zerumbet smith, family Zingiberaceae. Anti-cancer and apoptosis-inducing properties of zerumbone against various human tumour cells have been elucidated earlier. The aim of this study was to further evaluate in vitro, the cytogenetic and DNA damage e...

Palm date fruits have been used for nutritional and medicinal purposes in Middle Eastern countries. They are used in folk medicine for treatment of liver diseases and highly recommended to be consumed by pregnant women before and after delivery. Therefore, the present work aimed to determine the total phenolic content and total flavonoids in three...

Animals fed Catha edulis leaves develop an acute hepatitis and long-term feeding is associated with chronic active hepatitis and fibrotic liver disease. Repeated episodes of subclinical hepatitis with evolution to chronic liver disease has also been observed in patients chewing Catha edulis leaves. The aim of this study was to examine the effect of...

The present invention relates to a compound represented by the formula (I), method of preparation, pharmaceutical preparation containing said compound, and the use of said compound in the manufacture of a pharmaceutical preparation for the prevention and treatment of cancer by way of inhibiting the proliferation of carcinoma cells of the cervix and...

Zerumbone (ZER) is derived from Zingiber zerumbet smith from the Zingiberaceae family. It has been shown to have anti-cancer and apoptosis-inducing properties against various human tumour cells. The aim of our study was to assess the genotoxic effects of ZER in cultured human peripheral blood lymphocytes, Chinese Hamster Ovary (CHO) cells and rat b...

Zerumbone (ZER) is a natural compound isolated from Zingiber zerumbet smith, family Zingiberaceae. It has been demonstrated to have an in vitro antiproliferative effects against various human tumour cells as well as in vivo against a number of induced malignancies in mice. The aim of our study was to assess the genotoxicity of ZER in CHO cells and...

Abstract The high prevalence of lactase persistence (LP) among the people of Saudi Arabia is associated with the -13915(*)G variant allele upstream of the lactase gene (LCT). We, therefore, examined the frequency of the commonly known LP associated SNPs among randomly collected samples from Omani and Yemeni adult populations and obtained further da...

Palm date fruits have been used extensively for the traditional cure of liver illnesses and malaria in the Arab Peninsula. Therefore, the aim of the present study was to evaluate for the first time the in vivo hepatoprotective and the antioxidative effects of different varieties of palm date fruits in relation to their total phenolic contents and t...

The present invention relates to a compound represented by the formula (I), method of preparation, pharmaceutical preparation containing said compound, and the use of said compound in the manufacture of a pharmaceutical preparation for the prevention and treatment of cancer by way of inhibiting the proliferation of carcinoma cells of the cervix and...

Zerumbone (ZER) is derived from Zingiber zerumbet smith from the Zingiberaceae family. It has been shown to have anti-cancer and apoptosis-inducing properties against various human tumour cells. The aim of our study was to assess the effect of ZER on cisplatin-induced clastogenesis in Sprague-Dawley rat bone marrow polychromatic erythrocytes (PCEs)...

The use of evidence-based complementary and alternative medicine is increasing rapidly. Eleucine indica (EI) is traditionally used in ailments associated with liver and kidneys. The therapeutic benefit of the medicinal plants is often attributed to their antioxidant properties. Therefore, the aim of this study was to screen the hexane, dicholoromet...

The plant Typhonium flagelliforme, commonly known as "rodent tuber" in Malaysia, is often used as a health supplement and traditional remedy for alternative cancer therapies, including leukemia. This study aimed to evaluate in vitro anti-leukemic activity of dichloromethane extract/fraction number 7 (DCM/F7) from T. flagelliforme tuber on human T4...

Nigella sativa or Black seed (N. sativa L.) is traditionally used for several ailments in many Middle Eastern countries. It is an annual herbaceous plant that belongs to the Ranuculacea family with many beneficial properties as antitumor, antidiabetic, antihypertensive, antioxidative and antibacterial. This work attempted to study the effect of N....

Typhonium flagelliforme (TF) is a tropical plant, traditionally used by the ethnic population of Malaysia for the cure of various cancers. This plant had shown to induce antiproliferative effect as well as apoptosis in cancer cells. However, there is no available information to address that TF affects murine leukemia cells in vitro and in vivo. Her...

The plant Typhonium flagelliforme (TF), commonly known as 'rodent tuber' in Malaysia, is often used as traditional remedy for cancer, including leukemia. We had previously identified morphologically that the linoleic acid rich fraction (DCM/F7) from the tubers of this plant induces selective anti-proliferative effects and apoptosis in CEMss cells....

Type 2 diabetes mellitus (T2DM) is a worldwide public health problem. The fact that the existing treatments have limitations either because of their side effects as hypoglycaemia and weight gain or their other complications necessitate the need for development of new therapies for glycemic control. The therapeutic approachs represented by the incre...

The present invention relates to a compound represented by the formula (I), method of preparation, pharmaceutical preparation containing said compound, and the use of said compound in the manufacture of a pharmaceutical preparation for the prevention and treatment of cancer by way of inhibiting the proliferation of carcinoma cells of the cervix and...

The chromosomal aberrations (CA) assay and micronucleus (MN) test were employed to investigate the effect in vitro of zerumbone (ZER) on human chromosomes. ZER is a sesquiterpene compound isolated from the rhizomes of wild ginger, Zingiber zerumbet Smith. The rhizomes of the plant are employed as a traditional medicine for some ailments and as cond...

In the present study, callus was successfully obtained from hypocotyl explants of Brassica nigra (L.) in vitro nes, flavonoids and tannins. Although there were some exceptions, it was generally observed that total phenolic, antioxidant activity and antibacterial activity were higher in calli obtained under light incubation conditions than calli obt...

Zerumbone (ZER) is a sesquiterpene derived from Zingiber zerumbet smith, family Zingiberaceae. It has been shown to possess anti-cancer and apoptosis-inducing properties against various human tumour cells as well as in vivo against a number of induced malignancies in mice. In this study a simple, specific and accurate high performance liquid chroma...

Zerumbone (ZER) is derived from Zingiber zerumbet smith from the Zingiberaceae family. It has been shown to have anti-cancer and apoptosis-inducing properties against various human tumour cells. The aim of our study was to assess the genotoxic effects of ZER in cultured human peripheral blood lymphocytes, Chinese Hamster Ovary (CHO) cells and rat b...

Antibacterial, antioxidant, anticancer properties and chemical compositions of Goniothalamus umbrosus (GU) hexane extract was investigated using disc diffusion method, DPPH assay, MTT cytotoxicity test (MCF-7 breast cancer cell line, HT-29 colon cancer cell line and CEMss leukemia cell line) and GC-MS, respectively. Anti-tumor effect of GU was furt...

The present invention relates to a compound represented by the formula (I), method of preparation, pharmaceutical preparation containing said compound, and the use of said compound in the manufacture of a pharmaceutical preparation for the prevention and treatment of cancer by way of inhibiting the proliferation of carcinoma cells of the cervix and...

Cervical cancer is the second most common cause of cancer death in women. We have demonstrated previously that zerumbone (ZER) has an anti-cancer effect towards human cervical cancer cells (HeLa). Anti-cancer properties of ZER were investigated using female Balb/c mice exposed prenatally to diethylstilboestrol. Female offspring have been treated wi...

Recent in vitro and in vivo studies have demonstrated that zerumbone (ZER) possesses anticancer properties. The main objective of this study was to examine the effectiveness of the combination of ZER and cisplatin (CIS) to treat cervical intraepithelial neoplasia (CIN) in vivo. Microculture tetrazolium assay and immunohistochemistry of proliferatin...

Nigella sativa ( N. sativa ) is popularly known as the black seed, a herb that has traditionally been used for centuries in many parts of the world. It has gained popularity due to its potential health benefits. However, more scientific data is needed to support the various health claims. This study was carried out to determine the fatty acid profi...

Nigella sativa (N. sativa) is popularly known as the black seed, a herb that has traditionally been used for centuries in many parts of the world. It has gained popularity due to its potential health benefits. However, more scientific data is needed to support the various health claims. This study was carried out to determine the fatty acid profile...

This study was carried out to investigate the nutrients composition of Nigella sativa seed and its minerals content, cultivated in three different regions in Yemen, namely Marib, Sadah and Taiz. Proximate analysis, Total Dietary Fiber (TDF), Insoluble Dietary Fiber (IDF) and Soluble Dietary Fiber (SDF) were determined using the Association of Offic...

The aim of the current study is to determine the effects of A. indica aqueous extract on Diethyl Nitrosamine (DEN) and 2-Acetylaminofluorene (AAF) induced-hepatocarcinogenesis on Spraque-Dawley rats. The plant, A. indica, extract was prepared into 5% w/v in distilled water. Spraque-Dawley male rats were divided into 3 groups of 7 rats each. The gro...

The aim of the present study, is to evaluate the antimicrobial, phenolic content and free radical scavenging properties of the dichloromethane extract of Goniothalamus umbrosus leaves. The antimicrobial activities were evaluated using 2 Gram-positive bacteria, Methicillin Resistant Staphylococcus aureus (MRSA) and Bacillus subtilis B 29 and 2 Gram-...

This current study, aims to investigate the ethyl acetate and methanol extracts of Goniothalamus umbrosus for their anticancer effects on several human cancer cells namely, the MCF-7 breast cancer, HT-29 colon cancer and CEM-ss leukemia cell lines using a 3 days MTT (3-(4 and 5-dimethylthiazol-2-yl)-2 and 5-diphenyltetrazolium bromide) assay. Morph...

This present study aims to investigate whether Goniothalamus umbrosus extract has anticancer effects on several human cancer cells namely, the MCF-7 breast cancer cell line, HT-29 colon cancer cell line and CEM-ss leukemia cell line. Morphological changes and probable mode of cancer cell death induced by these G. umbrosus extract was examined. The...

Zerumbone is a natural compound isolated from the fresh rhizomes of Zingiber zerumbet. This bioactive compound has shown a chemo-preventive, anti-inflammatory and free radical scavenging activities. This study examines, the effect of zerumbone on the extent of tissue damage in Cisplatin-induced hepatotoxicity in rats. The rats received a single dos...

Zerumbone (ZER), a potential anticancer compound, isolated from the fresh rhizomes of Zingiber zerumbet. In this investigation, the cytotoxic properties of ZER were evaluated, on cancer cells of human cervix (HeLa), breast and ovary, and normal cells of Chinese Hamster ovary, using MTT assay. Apoptogenic effects of ZER on HeLa were studied using fl...

The aim of this study is to screen the hexane, dicholoromethane, ethyl acetate and methanol extracts of Pereskia bleo (PB) for their antibacterial, anti-oxidant and anti-cancer properties using disc diffusion method, DPPH assay and MTT cytotoxicity test, respectively. It is found that hexane and methanol extracts showed highly and moderately, respe...

Antibacterial, antioxidant, anticancer properties and chemical compositions of Goniothalamus umbrosus (GU) hexane extract was investigated using disc diffusion method, DPPH assay, MTT cytotoxicity test (MCF-7 breast cancer cell line, HT-29 colon cancer cell line and CEMss leukemia cell line) and GC-MS, respectively. Anti-tumor effect of GU was furt...

The aim of the present study, is to evaluate the antimicrobial, phenolic content and free radical scavenging properties of the dichloromethane extract of Goniothalamus umbrosus leaves. The antimicrobial activities were evaluated using 2 Gram-positive bacteria, Methicillin Resistant Staphylococcus aureus (MRSA) and Bacillus subtilis B 29 and 2 Gram-...

yphonium flagelliforme (Lodd.) Blume, commonly known as rodent tuber in Malaysia, is one of the widely used alternative medicines in cancer therapy by South East Asian population. Intake of this plant is common among patients with malignancies especially Leukaemia, breast and cervical cancer; however no data available regarding the possible direct...

The present invention relates to a compound represented by the formula (I), method of preparation, pharmaceutical preparation containing said compound, and the use of said compound in the manufacture of a pharmaceutical preparation for the prevention and treatment of cancer by way of inhibiting the proliferation of carcinoma cells of the cervix and...

The aim of this study was to evaluate the genotoxic effects of a crude extract of khat (Catha edulis, Forsk) leaves in rats. Two groups were fed khat crude extract, 1000 and 2000mg/kg body weight, for 90 days and were compared with a control group. The alkaline (pH>13) version of comet assay was used in this study. However, no previous published wo...

The aim of this study is to evaluate some derivatives of Zerumbone for their anti-tumor effects towards human cervical cancer cell lines (HeLa). The MTT tetrazolium salt colorimetric assay was utilized to evaluate the cytotoxic effects of ZER, cisplatin and the derivatives were n-Butylbenzene (compound 5), 1,1`-(4-Chlorobutylidene) bis(4-fluorobenz...

The aim of this study is to investigate the pharmacological effects of the methanolic extract of Clerodendron capitatum, CC, (family verbenaceae). These investigations were carried out on isolated frog rectus abdominus muscle and rabbit aortic strips and jejunum and non-pregnant rat uterus pretreated with oestradiol. Results showed that CC did not...

Problem Statement: Multiple drug resistance in human pathogenic micro organisms has developed due to indiscriminate use of modern antimicrobial drugs generally used in the management of infectious diseases. This increases the importance of exploiting the natural sources instead modern drugs. Approach: The antibacterial and antioxidant activity of d...

Cervical cancer is the second most common cancer of female reproductive tracts. In developing countries, cervical carcinoma is the leading cause of cancer fatality in women. Despite attempts to lower the fatality rate, very few in vivo models are in place to investigate this cancer. We therefore are able to develop an in vivo animal model that is s...

Herbal dugs could be a new source for inhibitors of acetyl cholinesterase (anti-AChE), the key enzyme in the breakdown of acetylcholine and a new talented approach for the cure of elderly neurologically associated disorders such as Alzheimer’s disease (AD). Zerumbone (ZER) is sesquiterpene from the edible plant, Zingiber zerumbet which is known to...

A natural compound, zerumbone was extracted, isolated and purified from the rhizomes of edible plant Zingiber zerumbet using methanol extraction and Column Chromatography (CC) method. The isolated and purified zerumbone crystals were subjected to High Performance Liquid Chromatography (HPLC), Liquid Chromatography Mass Spectrometry (LCMS) and 13C N...

The antioxidant and antibacterial activity of different extracts from of Typhonium flagelliforme (L.) Blume leaves (family: Araceae) commonly called �Rodent Tuber� was assessed towards different antioxidant models as well as in selected bacteria. None of the extracts showed significant activity against the selected strains. The only exception is he...

The aim of this study is to evaluate the anticancer and antimicrobial activities of zerumbone (ZER) from the rhizomes of Zingiber zerumbut. ZER is a crystalline sesquiterpene from the wild ginger, Z. zerumbet. This bioactive component has its unique structure, with a cross-conjugated ketone in an 11-membered ring, as well as remarkable biological a...

The leaves of khat (Catha edulis) are reported to have stimulating and pleasurable effects and are chewed habitually by people of East Africa and Arabian Peninsula. Due to various effects of khat the present study was undertaken to evaluate the short-term repeated dose effects of freeze dried khat leaves crude extract administration to male Sprague...

The antioxidant and antibacterial activity of different extracts from of Typhonium flagelliforme (L.) Blume leaves (family: Araceae) commonly called ‘Rodent Tuber’ was assessed towards different antioxidant models as well as in selected bacteria. None of the extracts showed significant activity against the selected strains. The only exception is he...

The aim of the current study is to determine the effects of A. indica aqueous extract on Diethyl Nitrosamine (DEN) and 2-Acetylaminofluorene (AAF) induced-hepatocarcinogenesis on Spraque-Dawley rats. The plant, A. indica, extract was prepared into 5% w/v in distilled water. Spraque-Dawley male rats were divided into 3 groups of 7 rats each. The gro...

Introduction: A natural compound, zerumbone was extracted, isolated and purified from the rhizomes of edible plant Zingiber zerumbet using methanol extraction and column chromatography method. The purified zerumbone has been investigated for its anti-cancer properties on human cervical cancer cell line (HeLa) and compared to cisplatin, the current...

Zingiber zerumbut is local plants that found to anti-inflammatory, antiviral and chemo-preventive properties. The compound Zerumbone was isolated from this plant and it was tested in the laboratories to investigate it is anti-cancer potentiality in vivo and in vitro cervical cancer models. The compound was found to have a regressive effects on the...

The leaves of khat (Catha edulis) are found to have stimulating and pleasurable effects and are chewed habitually by people of East Africa and Arabian Peninsula. Due to the habitual widespread use of khat in many African countries and the Yemen and in order to investigate the effects of khat on various biochemical parameters, the present study was...

The effect of regular khat (Catha edulis) chewing on plasma lipid peroxidation as a biomarker of oxidative stress and free radical activity (measured as plasma malondialdehyde, MDA), as well as on the lipid profile were investigated in male and female Yemeni khat chewers. Plasma levels of MDA were observed to be sex related, being significantly low...

Khat chewing is a widespread habit that has a deep-rooted sociocultural tradition in Africa and the Middle East. The leaves of khat (Catha edulis) are found to have stimulating and pleasurable effect and are chewed habitually by people of East Africa and Arabian Peninsula. Due to various toxic and psychostimulative effect of khat the present study...

The effect of regular khat (Catha edulis) chewing and the combination of khat chewing and smoking on plasma lipid peroxidation as a biomarker of oxidative stress and free radical activity (measured as plasma malondialdehyde, MDA), as well as on the lipid profiles were investigated. The fasting plasma levels of MDA were non-significantly higher in b...