Adam Kuzdraliński

Adam Kuzdraliński
University of Life Sciences in Lublin · Department of Biotechnology, Human Nutrition and Science of Food Commodities

DSc/Habilitation

About

36
Publications
9,783
Reads
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264
Citations
Citations since 2017
23 Research Items
225 Citations
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201720182019202020212022202301020304050
201720182019202020212022202301020304050
201720182019202020212022202301020304050
Additional affiliations
October 2011 - present
University of Life Sciences in Lublin
Position
  • Researcher

Publications

Publications (36)
Article
Full-text available
Fungal cereal pathogens, including Blumeria graminis f.sp. avenae, have the ability to adapt to specific conditions, which in turn leads to overcoming host resistance. An important aspect is the standardized way of characterizing the races and pathotypes of the pathogen. In the presented work, for the first time it was proposed to use a unified let...
Article
Full-text available
Zymoseptoria tritici is a fungal pathogen causing losses in wheat yields. Here, we present new primer sets for species-specific identification of this microorganism in wheat leaf samples using conventional PCR. Primer sets were validated in silico with the use of tools available in genetic databases. Furthermore, in vitro tests were also carried ou...
Article
Full-text available
Background: Quantitative PCR (qPCR) is one of the most common and accurate methods of gene expression analysis. However, the biggest challenge for this kind of examinations is normalization of the results, which requires the application of dependable internal controls. The selection of appropriate reference genes (RGs) is one of the most crucial p...
Article
Full-text available
Enterobacter aerogenes LU2 was isolated from cow rumen and recognized as a potential succinic acid producer in our previous study. Here, we present the first complete genome sequence of this new, wild strain and report its basic genetic features from a biotechnological perspective. The MinION singlemolecule nanopore sequencer supported by the Illum...
Preprint
Full-text available
Background Quantitative PCR (qPCR) is one of the most common and accurate methods of gene expression analysis. However, the biggest challenge for this kind of examinations is normalization of the results, which requires the application of dependable internal controls. The selection of appropriate reference genes (RGs) is one of the most crucial poi...
Preprint
Full-text available
Background Quantitative PCR (qPCR) is one of the most common and accurate methods of gene expression analysis. However, the biggest challenge for this kind of examinations is normalization of the results, which requires the application of dependable internal controls. The selection of appropriate reference genes (RGs) is one of the most crucial poi...
Article
Full-text available
We developed new PCR assays that target beta-tubulin (TUB2) and 14 alpha-demethylase (CYP51) genes and used them for the species-specific detection of Blumeria graminis f. sp. tritici (Bgt). Based on fungi DNA sequences available in the NCBI (National Center for Biotechnology Information) GenBank database we developed a simplex and duplex PCR assay...
Article
Full-text available
The ability of Rhizopus oryzae to produce fumaric acid in the presence of glycerol and/or various monosaccharides as carbon sources was examined for seventeen different strains of this fungi. These strains were tested in shake-flask cultures on media containing glycerol and seven different carbohydrates, including glucose, fructose, galactose, mann...
Article
Full-text available
The species Puccinia triticina (Pt) and Puccinia striiformis f. sp. tritici (Pst) are devastating cereal pathogens that cause leaf and stripe rust diseases. We developed PCR assays for the species-specific detection of Pt and Pst, 2 biological agents that cause wheat rust disease. For each pathogen, we validated 3 primer sets that target the second...
Article
Full-text available
Polyalcohol arabitol can be used in the food and pharmaceutical industries as a natural sweetener, a dental caries reducer, and texturing agent. Environmental samples were screened to isolate effective yeast producers of arabitol. The most promising isolate 27RL-4, obtained from raspberry leaves, was identified genetically and biochemically as Cand...
Conference Paper
Full-text available
Wheat powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of the most destructive and reemerging foliar diseases worldwide. Despite its significance, the ability to detect and identify this fungal pathogen at its early asymptomatic developmental stages is still limited. In this study, we developed new primer sets targeting beta-t...
Article
Full-text available
Arabitol is a polyalcohol which has about 70% of the sweetness of sucrose and an energy density of 0.2 kcal/g. Similarly to xylitol, it can be used in the food and pharmaceutical industries as a natural sweetener, a texturing agent, a dental caries reducer, and a humectant. Biotechnological production of arabitol from sugars represents an interesti...
Article
Full-text available
Infection of phyllosphere (stems, leaves, husks, and grains) by pathogenic fungi reduces the wheat yield and grain quality. Detection of the main wheat pathogenic fungi provides information about species composition and allows effective and targeted plant treatment. Since conventional procedures for the detection of these organisms are unreliable a...
Article
Full-text available
Fusarium populations were investigated on 53 samples of wheat grains and husks collected approximately three weeks before harvest in 53 wheat fields in south-eastern Poland. A limited area of sample collection was chosen intentionally to avoid the effect of climate and weather variability. The study was conducted to assess the occurrence of 13 Fusa...
Article
Fungal pathogens are a major problem in the production of cereals, including common wheat (Triticum aestivum L.). They cause physiological damages of plants as well as cereal quality loss by mycotoxin contamination. Economic losses in wheat production imply the identification of pathogenic fungi at the beginning of the growing season and subsequent...
Article
Full-text available
The causal agent of Septoria tritici blotch (STB) is the fungus Mycosphaerella graminicola. Polymerase chain reaction (PCR) was used for the early detection of M. graminicola genetic material in 160 samples of winter wheat leaves from Poland, collected in the spring and autumn of 2012. Difference was noted between the number of detections in autumn...
Article
Full-text available
Foot rot and crown rot are fungal diseases of wheat caused by a complex of Fusarium species. They have a huge economic impact mainly due to yield reduction. A survey was conducted to identify four Fusarium species, occurring on wheat stem bases, using speciesspecific PCR assays in samples collected during spring of 2012. The dominant species was F....
Article
In this study, we investigated the influence of organic and conventional farming systems on mycotoxin levels in oats from 2006 to 2008. The relationship between mycotoxin contents was also calculated. Data were analysed separately for each factor (year - three values and production systems - two values) and their combinations, e.g. analysis of samp...
Article
Full-text available
Mycotoxins have a proven toxic effect on the health of humans and animals. Nowadays, there is a focus on having a healthy lifestyle and consuming organic foods. High quality grain products, especially spelt products, which are an important element of a well-balanced diet have become more popular. The aim of this study was to determine the mycotoxin...
Article
Causal agents of Fusarium head blight (FHB) in organic spring barley protected with biopreparations and contamination of the grain with deoxynivalenol, T-2 toxin, zearalenone, aflatoxins and ochratoxin A were analysed. The content of mycotoxins was defined by ELISA. The main cause of spring barley FHB was Fusarium poae, and accompanying species wer...
Article
Full-text available
Cause of Fusarium head blight (FHB) in organic winter rye protected by biopreparations and contamination of the grain with the deoxynivalenol, T-2 toxin, zearalenone, aflatoxins and ochratoxin A were analysed. Biochicol 020 PC and biopre-paration made in laboratory on the ground chitosane and Fusarium culmorum were used in field experiment. Content...
Article
Full-text available
Cause of Fusarium head blight (FHB) in organic triticale protected by biopreparations and contamination of the grain with the deoxynivalenol, T-2 toxin, zearalenone, aflatoxins and ochratoxin A were analysed. Biochicol 020 PC and bioprepara-tion made in laboratory on the ground chitosane and Fusarium culmorum were used in field experiment. Content...
Article
Content of mycotoxins in grain of triticale cultivars grown in organic and con-ventional system of production was estimated. From among 13 examined cultivars of organic triticale deoxynivalenol was identified in all samples (up to 0.32 mg/kg) and T-2 toxin was detected in 11 samples (< 75 g/kg). From among 13 examined cultivars of conventional trit...

Questions

Questions (2)
Question
Commercial ELISA kits are dedicated to making assessment in samples from cereals and feed. My colleague wants to do an assessment in beers using this test. He told me that he will check only a kit's reproducibility - he will do few determinations in one sample and compare them - if the kit proves to be reproductible, it is fine. For me, it is not fine because if the kit was not used by the manufacturer to determine sth in beer, we really don't know how antibodies will work in new "environment". For me he should do, for example, HPLC determination of this metabolite in at least few samples to compare results with this ELISA kit (for me best way will be determination using both methods in samples with low, middle and high concentration and also check recovery i.e. after addition of known amount of standard before sample preparation). In my research I was avoiding using ELISA kits which were not made specifically for some product (i.e. wine). Am I right or am I being too detailed?
Question
I am working with NCBI BLAST to compare few aa sequences with database records. I can choose, after analysis, the option "Distance tree of results". I know that we can choose results for each sequence separately but is there an option to draw a tree for all sequences (on one tree). I know that I can copy similar sequences from databases to MEGA (for example) and make an analysis using this software but it will be very "painful" and time-consuming to do. Is there any possibility to do it with NCBI tools or other on line scripts?

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Projects

Project (1)
Archived project
The aim of the project is to develop new molecular diagnostic tests to enable the rapid identification of key fungal pathogens in common wheat. This innovative approach will enable the targeted use of pesticides. In addition, tests will be designed to be used in the process of evaluating the effectiveness of plant protection products (replacing visual assessment).