Adalberto Luiz Rosa

• Professor
• University of São Paulo

This study evaluated the impact of a single variation in the etching time of H2SO4/H2O2-treated titanium (Ti) surfaces on the adsorption of growth and differentiation factor-5 (GDF-5) and their effects on the acquisition of the osteogenic phenotype in vitro. Rat primary calvarial osteogenic cells were grown for up to 14 days on the following Ti sur...

Adipose tissue is an attractive source of mesenchymal stem cells (at‐MSCs), but their low osteogenic potential limits their use in bone regeneration. Adipose tissue plays a role in pro‐inflammatory diseases by releasing cytokines with a catabolic effect on bone, such as tumor necrosis factor‐alpha (TNF‐α). Thus, we hypothesized that endogenous TNF‐...

Type 1 (T1DM) and type 2 (T2DM) diabetes mellitus are characterized by changes in glucose metabolism and cause bone damage via a variety of mechanisms, including effects on osteoblasts. We aimed to evaluate the osteoblast differentiation of mesenchymal stem cells (MSCs) from rats with T1DM or T2DM and the effects of removing the hyperglycemic stimu...

Background: Cell therapy based on the use of mesenchymal stromal cells (MSCs) is a strategy of regenerative medicine to treat large bone defects and the study of proteins from these cells that can favor bone regeneration is essential in this context. The extracellular matrix protein agrin is involved in several biological processes and it has been...

Despite the crucial role of osteoclasts in the physiological process of bone repair, most bone tissue engineering strategies have focused on osteoblast-biomaterial interactions. Although Biosilicate® with two crystalline phases (BioS-2P) exhibits osteogenic properties and significant bone formation, its effects on osteoclasts are unknown. This stud...

Cell therapy is a valuable strategy for the replacement of bone grafts and repair bone defects, and mesenchymal stem cells (MSCs) are the most frequently used cells. This study was designed to genetically edit MSCs to overexpress bone morphogenetic protein 9 (BMP-9) using Clustered Regularly Interspaced Short Palindromic Repeats/associated nuclease...

Osteoporosis is characterized by decreased bone mass and adipocyte accumulation within the bone marrow that inhibits osteoblast maturation, leading to a high risk of fractures. Thus, we hypothesized that osteoblasts, besides being negatively affected by interacting with adipocytes, reduce the differentiation of neighboring osteoblasts through the s...

Aim: The aim of this study was to investigate the effect of local injection of osteoblasts differentiated from bone marrow (BM-OB) or adipose tissue (AT-OB) mesenchymal stromal cells on bone tissue formation. Materials & methods: Defects were created in rat calvaria and injected with BM-OB or AT-OB and phosphate-buffered saline without cells were i...

Among bone morphogenetic proteins (BMP), BMP-9 has been described as one of the highest osteogenic potential, also presenting angiogenic potential. The aim of this study was to evaluate the in vitro concentration of BMP-9 to favor angiogenic and osteogenic potentials of bone marrow- and adipose tissue-mesenchymal stem cells from (BM-MSC) (AT-MSC).

Treatment of large bone defects is a challenging clinical situation that may be benefited from cell therapies based on regenerative medicine. This study was conducted to evaluate the effect of local injection of bone marrow-derived mesenchymal stromal cells (BM-MSCs) or adipose tissue-derived MSCs (AT-MSCs) on the regeneration of rat calvarial defe...

Wnt/β-catenin signal transduction is involved in the homeostatic control of bone mass. It is well established that a titanium surface with nanotopography (Ti-Nano) favors osteoblast differentiation by modulating different signaling pathways. However, few studies have investigated the participation of the Wnt/β-catenin pathway in the osteogenic effe...

The major role of integrins is to mediate cell adhesion but some of them are involved in the osteoblasts‐titanium (Ti) interactions. In this study, we investigated the participation of integrins in osteoblast differentiation induced by Ti with nanotopography (Ti‐Nano) and with microtopography (Ti‐Micro). By using a PCR array, we observed that, comp...

A quantity of 3 mol% yttria‐partially stabilized zirconia (Y‐TZP) with 10.5 and 19.9 vol% 64S bioglass compacts was sintered at different temperatures up to 1500°C. The influence of 64S glass addition on the sintering kinetic, flexural strength, and osteoblast cell response of Y‐TZP ceramics was investigated. The addition of 64S glass increased the...

Introducción. Los adipocitos (ADs) inhibem la diferenciación osteoblástica por sintetizar y secretar la citocina (TNF𝛂)1. Objetivo. Investigar el efecto del medio condicionado (CM) por osteoblastos (OBs) cocultivados com ADs sobre la expresión de citocinas inflamatórias y marcadores oseos y adipogénicos por OBs cultivados en medio no condicionado...

Quantitative real-time polymerase chain reaction (qRT-PCR) is a powerful tool to evaluate gene expression, but its accuracy depends on the choice and stability of the reference genes used for normalization. In this study, we aimed to identify reference genes for studies on osteoblasts derived from rat bone marrow mesenchymal stem cells (bone marrow...

Among bone morphogenetic proteins (BMPs), BMP‐9 has been described as one with higher osteogenic potential. Here, we aimed at evaluating the effect of BMP‐9 on the osteoblast differentiation of cells grown on titanium (Ti) with nanotopography, a well‐known osseoinductive surface. MC3T3‐E1 cells were grown either in absence or presence of BMP‐9 (20...

Highly porous zirconia (ZrO2) scaffolds fabricated by the replication method were coated with fluorapatite (FA). The FA coating was obtained by dipping the ZrO2 scaffolds into stabilized aqueous FA slips having different viscosity values (≤ 5.0mPa.s). The influence of the FA slip viscosity and the immersion time on the reduction of the scaffold por...

One of the tissue engineering strategies to promote bone regeneration is the association of cells and biomaterials. In this context, the aim of this study was to evaluate if cell source, either from bone marrow or adipose tissue, affects bone repair induced by osteoblastic cells associated with a membrane of poly(vinylidene-trifluoroethylene)/bariu...

Osteoblasts and adipocytes coexist in the implantation site and affect the process of titanium (Ti) osseointegration. As extracellular signal-regulated kinases 1/2 (ERK1/2) are involved in osteogenesis and adipogenesis, the aim of our study was to investigate if the effects of Ti surface topography on osteoblast and adipocyte differentiation are mo...

Several studies have shown the positive effects of Ti either with nanotopography or coated with collagen on osteoblast differentiation. Thus, we hypothesized that the association of nanotopography with collagen may increase the in vitro osteogenesis on Ti surface. Ti discs with nanotopography with or without collagen coating were characterized by s...

Investigation on functional genome research may contribute to the knowledge of functional roles of different mRNAs and miRNAs in bone cells of osteoporotic animals. Currently, few studies indicate the changes in gene modulation that osteoporosis causes in osteoblastic cells from different sites. Thus, the purpose of this investigation was to evalua...

Osteoporosis is a chronic disease that impairs proper bone remodeling. Guided bone regeneration is a surgical technique that improves bone defect in a particular region through new bone formation, using barrier materials (e.g. membranes) to protect the space adjacent to the bone defect. The polytetrafluorethylene membrane is widely used in guided b...

Para o reparo de defeitos ósseos, o uso da engenharia de tecidos, combinando células e biomateriais, tem se mostrado uma alternativa promissora. Nesse estudo, o arcabouço de Biosilicato® com duas fases cristalinas (Bio 2P) foi avaliado in vitro para determinar seu potencial osteogênico e osteoindutor e in vivo foi associado ou não a células para in...

The ability of Biosilicate® with two crystalline phases (BioS-2P) to drive osteoblast differentiation encourages the investigation of the cellular mechanisms involved in this process. Then, the aim of our study was to analyze the large-scale gene expression of osteoblasts grown on BioS-2P compared with Bioglass®45S5 (45S5). Osteoblasts differentiat...

Aim: To evaluate the effect of a calcium aluminate-based cement (CAC+) on the development of the osteogenic phenotype in vitro. Methodology: Rat calvaria-derived cells were grown on Thermanox(®) coverslips for 24 h and then exposed either to samples (4-hour set) of CAC+ or mineral trioxide aggregate (MTA) placed on Transwell(®) inserts for perio...

Objective We investigated the osteoblast and adipocyte differentiation of mesenchymal stem cells (MSCs) from young and aged rats cultured on Ti. Material and Methods Bone marrow MSCs derived from 1-month and 21-month rats were cultured on Ti discs under osteogenic conditions for periods of up to 21 days and osteoblast and adipocyte markers were ev...

The poly(vinylidene-trifluoroethylene)/barium titanate (PVDF) membrane enhances in vitro osteoblast differentiation and in vivo bone repair. Here, we hypothesized that this higher bone repair could be also due to bone resorption inhibition mediated by a miR/RANKL circuit. To test our hypothesis, the large-scale miR expression of bone tissue grown o...

This study evaluated the temporal expression of osteopontin (OPN) in co-cultures of human osteoblastic cells (SAOS-2) and oral squamous cell carcinoma (OSCC)-derived cells (SCC9) and examined the effects of osteoblast-derived OPN on the neoplastic cell phenotype. Additionally, the effects of these co-cultures on subsequent osteoclastic activity wer...

Participation of microRNA-34a/RANKL in the osteogenic potential of the Poly(vinylidene-trifluorethylene)/barium titanate membrane

Two commercial 3mol% yttria-partially stabilized zirconia powders, with 0.3wt% Al2O3 (YZA) and without Al2O3 (YZ), were used to produce alumina (Al2O3)-zirconia (ZrO2) slip cast composites. The influence of the ZrO2 content and the ZrO2 grain size on the ageing behavior of the different Al2O3-ZrO2 composites was investigated. In addition, the in vi...

Bioactive glass-ceramics have emerged as promising biomaterials to produce scaffolds for bone tissue engineering thanks to their physical properties and stimulatory effects on osteoblast differentiation. Among the biomaterials, bioactive glass-ceramics emerge as a promising scaffold due to their physical properties and the stimulatory effects on os...

Three dimensional, highly porous, ZrO2 scaffolds coated by glass-ceramic derived from 45S5 bioglass were fabricated. The surface reactivity of 45S5 in aqueous solution was investigated as a function of the immersion time. The influence of the solid loading on the rheological behavior of 45S5 aqueous slips with ammonium polyacrylate (NH4PA) was stud...

Objective In this context, the aim of this study was to compare the potential of bone repair of collagen sponge with fibrin glue in a rat calvarial defect model. Material and Methods Defects of 5 mm in diameter were created in rat calvariae and treated with either collagen sponge or fibrin glue; untreated defects were used as control. At 4 and 8 w...

Introduction. Bone tissue repair can be achieved through tissue engineering strategies where cells are carried to bone defects within a porous scaffold. In this study we evaluated the effect of combining mesenchymal stem cells (MSCs) or osteoblasts (OBs) with a bioactive glass scaffold (Bio-sca) on repair of bone defects created in rat calvarias. M...

The present study evaluated whether the changes in the labeling pattern of cytoskeletal proteins in osteogenic cells cultured on bioactive glass-based materials are due to altered mRNA and protein levels. Primary rat-derived osteogenic cells were plated on Bioglass® 45S5, Biosilicate®, and borosilicate (bioinert control). The following parameters w...

The aim of this study was to evaluate the in vitro and in vivo osteogenic potential of the bioactive glass-ceramic scaffold (Bio-Sca). Rat mesenchymal stem cells (MSCs) were cultured on either dense Bio-Sca discs and or polystyrene (control) under non-osteogenic conditions. At day 10, alkaline phosphatase (ALP) activity and gene expression of the b...

Clinical success of implant therapy is directly related to titanium (Ti) surface properties and the quality of bone tissue. The treatment of Ti implants with H2SO4/H2O2 is a feasible, reproducible and low-cost technique to create surface nanotopography (Ti-Nano). As this nanotopography induces osteoblast differentiation, we hypothesized that it may...

The effects of hydrothermal and phosphoric acid treatments on the tetragonal ZrO2 stability and surface microstructure were determined for 3 mol% yttria- partially stabilized zirconia (Y-TZP) and Al2O3 doped Y-TZP (Y-TZPA). Y-TZP slip cast compacts were sintered at 1300–1500 °C to achieve fully dense samples. The increase in the sintering temperatu...

Mesenchymal stem cells from bone marrow (BM-MSCs) and adipose tissue (AT-MSCs) are attractive tools for cell-based therapies to repair bone tissue. In this study, we investigated the osteogenic and adipogenic potential of BM-MSCs and AT-MSCs as well as the effect of crosstalk between osteoblasts and adipocytes on cell phenotype expression. Rat BM-M...

This study was designed to investigate the influence of mesenchymal stem cells (MSCs) on osteoblast (OB) differentiation. Rat bone marrow MSCs were cultured either in growth medium that maintained a MSC phenotype or in osteogenic medium that induced differentiation into OBs. Then, cells were grown in two different culture conditions: indirect co-cu...

The aim of this study was to investigate if chemically produced nanotopography on titanium (Ti) surface induces osteoblast differentiation of cultured human bone marrow mesenchymal stem cells (hMSCs) by regulating the expression of microRNAs (miRs). It was demonstrated that Ti with nanotopography induces osteoblast differentiation of hMSCs as evide...

Objective: Fresh-frozen bone allograft (FFBA) is an alternative to autogenous bone (AB) for reconstructing maxillary bone. Despite the promising clinical results, cell responses to FFBA and AB were not evaluated. Thus, our aim was to compare cells harvested from maxillary reconstructed sites with either AB or FFBA in terms of osteoblast differenti...

The bioactive glass 45S5 favours osteoblast activities resulting in enhanced osteogenesis both in vitro and in vivo1, but the relative poor mechanical properties restrict its use as bone substitute 2,3. In this context, novel full-crystallized bioactive glass-ceramics, Biosilicate® (Bio) and Biosilicate® for scaffold fabrication (Bio-sca), has been...

The increasing clinical demand for bone substitutes has driven significant progress in bone tissue engineering. Among the biomaterials that have been employed as scaffolds, bioactive glasses emerge as a novel alternative due to their high biocompatibility and ability to stimulate in vitro osteogenesis. Despite the advantages on bone healing, the us...

In this study we evaluated the effect of new plasma-nitrided Ti surfaces on the progression of osteoblast cultures, including cell adhesion, proliferation and differentiation. Ti surfaces were treated using two plasma nitriding protocols, hollow cathode for 3 h (HC 3h) and 1 h (HC 1h) and planar (Planar) for 1 h. Untreated Ti surfaces were used as...

This chapter provides a review of recent advances in understanding the importance of normal mesenchymal stem cell (MSC) differentiation and the key regulators that orchestrate their fate into several cell types. Human bone marrow and umbilical cord veins are sources that achieve enough quantities of MSCs, which differentiate in vitro into osteoblas...

Bone tissue engineering is highly dependent of osteoblastic differentiation that is influenced by the cell growing substrates. Here we compared the osteoblastic differentiation of cells grown on distinct glass-based substrates in terms of chemical composition and thermal treatments. MC3T3-E1 pre-osteoblastic cells were cultured under osteogenic con...

Bone tissue engineering is highly dependent of osteoblastic differentiation that is influenced by the cell growing substrates. Here we compared the osteoblastic differentiation of cells grown on distinct glass-based substrates in terms of chemical composition and thermal treatments. MC3T3-E1 pre-osteoblastic cells were cultured under osteogenic con...

Bone tissue engineering is highly dependent of osteoblastic differentiation that is influenced by the cell growing substrates. Here we compared the osteoblastic differentiation of cells grown on distinct glass-based substrates in terms of chemical composition and thermal treatments. MC3T3-E1 pre-osteoblastic cells were cultured under osteogenic con...

Bone tissue engineering is highly dependent of osteoblastic differentiation that is influenced by the cell growing substrates. Here we compared the osteoblastic differentiation of cells grown on distinct glass-based substrates in terms of chemical composition and thermal treatments. MC3T3-E1 pre-osteoblastic cells were cultured under osteogenic con...

In this study, we evaluated the effect of poly(vinylidene fluoride-trifluoroethylene)/barium titanate (P(VDF-TrFE)/BT) membrane on in vivo bone formation. Rat calvarial bone defects were implanted with P(VDF-TrFE)/BT and polytetrafluoroethylene (PTFE) membranes, and at 4 and 8 weeks, histomorphometric and gene expression analyses were performed. A...

Abstract Despite advances in bone reconstruction and rehabilitation techniques, treatment of partially edentulous patients with atrophic anterior maxilla remains a challenge. Alveolar osteogenic distraction and allografts have been used as an alternative to autografts, avoiding use of a donor area, while minimizing morbity of the procedure. The aim...

Objectives: The aims of this research were to evaluate the efficacy of a bioactive glass- ceramic (Biosilicate s ) and a bioactive glass (Biogran s ) placed in dental sockets in the maintenance of alveolar ridge and in the osseointegration of Ti implants. Material and methods: Six dogs had their low premolars extracted and the sockets were implante...

Cell culture system has been used to evaluate alloy cytotoxicity under different environments, testing the extracts, but the effect of temperature variation on the cytotoxicity of dental alloys has not been analyzed. The aim of the present study was to investigate if temperature variation could affect dental alloy cytotoxicity, testing alloy extrac...

Objectives: Autografts from mandibular symphysis and ramus are often used for bone reconstruction. Based on this, we hypothesized that these sites could be useful cell sources for bone tissue engineering approaches. Thus, our study aimed at evaluating the proliferation and osteoblast phenotype development of cells derived from mandibular symphysis...

Objectives: This study aimed to comparatively evaluate the in vitro osteogenic potential of cells obtained from the mandibular ramus (MR, autogenous bone donor site) and from the maxillary sinus (MS) bone grafted with a mixture of anorganic bovine bone (ABB) and MR prior to titanium implant placement (MS, grafted implant site). Material and metho...

A myriad of titanium (Ti) surface modifications has been proposed to hasten the osseointegration. In this context, the aim of this study was to perform histomorphometric, cellular, and molecular analyses of the bone tissue grown in close contact with Ti implants treated by anodic spark deposition (ASD-AK). Acid-etched (AE) Ti implants either untrea...

PURPOSE: Cyanoacrylate has been used as a commercial tissue adhesive. Recently, ethyl 2-cyanoacrylate has been suggested for the fixation of onlay autogenous bone graft. However, ethyl 2-cyanoacrylate must be biocompatible with bone tissue. This study evaluated the cytotoxicity of cyanoacrylate adhesives using a direct contact assay on human oral o...

Background: The functionalization of metallic surfaces aims at promoting the cellular response at the biomaterial-tissue interface. This study investigates the effects of the functionalization of titanium (Ti) microtopography with a calcium phosphate (CaP) coating with and without peptide 15 (P-15), a synthetic peptide analog of the cell-binding d...

Objectives: Vascular endothelial growth factor (VEGF) is a glycoprotein that induces physiological and pathological angiogenesis and increases permeability of blood vessels, essential for tissue repair. In dental pulp, VEGF expression is related to regulation of angiogenesis, revascularization and dentinogenesis. Low-level laser therapy (LLLT) is r...

Objective: The aim of the present study was to evaluate the progression of osteogenic cell cultures exposed to a novel calcium aluminate cement (CAC+). Methods: Osteogenic cells were enzimatically isolated from newborn rat calvarial bone and plated on Thermanox coverslips at 20,000 cells/well. CAC+ and mineral trioxide aggregate (MTA) specimens (4...

The investigation of titanium (Ti) surface modifications aiming to increase implant osseointegration is one of the most active research areas in dental implantology. This study was carried out to evaluate the benefits of coating Ti with type I collagen on the osseointegration of dental implants. Acid etched Ti implants (AETi), either untreated or c...

The objective of this study was to compare the bone repair along a mandibular body osteotomy stabilized with 2.0mm absorbable and metallic systems. 12 male, adult mongrel dogs were divided into two groups (metallic and absorbable) and subjected to unilateral osteotomy between the mandibular third and fourth premolars, which was stabilized by applyi...

The aim of this study was to characterize the physicochemical properties of bacterial cellulose (BC) membranes functionalized with osteogenic growth peptide (OGP) and its C-terminal pentapeptide OGP[10-14], and to evaluate in vitro osteoinductive potential in early osteogenesis, besides, to evaluate cytotoxic, genotoxic and/or mutagenic effects. Pe...

This study investigated the effect of pore size on osteoblastic phenotype development in cultures grown on porous titanium (Ti). Porous Ti discs with three different pore sizes, 312 μm (Ti 312), 130 μm (Ti 130) and 62 μm (Ti 62) were fabricated using a powder metallurgy process. Osteoblastic cells obtained from human alveolar bone were cultured on...

Objective: The aim of this study was to evaluate the effect of a novel calcium aluminate cement (CAC+) on gene expression of key osteoblastic markers in primary osteogenic cell cultures. Method: Osteogenic cells were enzimatically isolated from newborn rat calvarial bone, plated on Thermanox coverslips at 20,000 cells/well, and cultured for up to...

The purpose of this study was to analyze the influence of two different irradiation times with 85 mW/cm(2) 830 nm laser on the behavior of mouse odontoblast-like cells. The use of low-level laser therapy (LLLT) to stimulate pulp tissue is a reality, but few reports relate odontoblastic responses to irradiation in in vitro models. Odontoblast-like c...

A common subject in bone tissue engineering is the need for porous scaffolds to support cell and tissue interactions aiming at repairing bone tissue. As poly(lactide-co-glycolide)-calcium phosphate (PLGA-CaP) scaffolds can be manufactured with different pore sizes, the aim of this study was to evaluate the effect of pore diameter on osteoblastic ce...

Several biological events are controlled by Hedgehog (Hh) signaling, including osteoblast phenotype development. This study aimed at evaluating the gene expression profile of human mesenchymal stem cells (hMSCs) treated with the Hh agonist, purmorphamine, focusing on Hh signaling and osteoblast differentiation. hMSCs from bone marrow were cultured...

We tested the hypothesis that the osteoblast differentiation status of bone marrow stem cells (BMSCs) combined with a three-dimensional (3D) structure modulates bone formation when autogenously implanted. Rat BMSCs were aspirated, expanded, and seeded into a 3D composite of poly(lactide-co-glycolide) and calcium phosphate (PLGA/CaP) to produce a hy...

The aim of this study was to investigate the osteoblastic activity of cells derived from the midpalatal suture upon treatment with low-level laser therapy (LLLT) after rapid maxillary expansion (RME). A total of 30 rats were divided into two groups: experimental I (15 rats with RME without LLLT) and experimental II (15 rats with RME + LLLT). The ra...

Extracellular matrix proteins are key factors that influence the regenerative capacity of tissues. The objective of the present study was to evaluate the effects of enamel matrix derivative (EMD), TGF-β1, and the combination of both factors (EMD+TGF-β1) on human osteoblastic cell cultures. Cells were obtained from alveolar bone of three adult patie...

A promising new treatment in dentistry involves the photodynamic process, which utilizes a combination of two therapeutic agents, namely a photosensitizer drug and a low dose of visible light. We investigated the in vitro effect of low intensity laser irradiation (visible light irradiation at 670 nm) using doses ranging between 0.5 and 3 J/cm(2), c...

This study investigated the response of human alveolar bone-derived cells to a novel poly(vinylidene fluoride-trifluoroethylene)/barium titanate (P(VDF-TrFE)/BT) membrane. Osteoblastic cells were cultured in osteogenic conditions either on P(VDF-TrFE)/BT or polytetrafluoroethylene (PTFE) for up to 14 days. At 7 and 14 days, the mRNA expression of R...

Objectives: The aim of this study was to investigate the effects of a novel calcium aluminate cement (CAC+), which has been developed to overcome the negative features of the MTA, on the early events of the progression of the osteogenic phenotype in vitro. Methods: CAC+ and MTA samples (4 mm in diameter and 2 mm height) were fixed in the center of...

Aim: This study aimed at determining the effect of different thickness of porous titanium (Ti) coating, 0.5, 1.0 and 1.5 mm thick (PC-0.5, PC-1.0 and PC-1.5), on osteoblast phenotype expression. Materials and methods: Dense Ti discs coated with 0.5, 1.0 and 1.5 mm of porous Ti (PC-0.5, PC-1.0 and PC-1.5, respectively) were fabricated by powder meta...

The present study evaluated the progression of osteogenic cell cultures exposed to a novel calcium aluminate cement (CAC+) in comparison with the gold standard mineral trioxide aggregate (MTA). Cells were enzimatically isolated from newborn rat calvarial bone, plated on glass coverslips containing either CAC+ or a control MTA samples in the center,...

The bone-biomaterial interface has been characterized by layers of afibrillar extracellular matrix (ECM) enriched in non collagenous proteins, including osteopontin (OPN), a multifunctional protein that in bone controls cell adhesion and ECM mineralization. Physical and chemical aspects of biomaterial surfaces have been demonstrated to affect cell-...