Ada Wróblewska

• PhD
• Professor (Associate) at University of Białystok

Background: The patterns of inbreeding coefficients (FIS) and fine spatial genetic structure (FSGS) were evaluated regarding the mating system and inbreeding depression of food-deceptive orchids, Dactylorhiza majalis, Dactylorhiza incarnata var. incarnata, and Dactylorhiza fuchsii, from NE Poland. Methods: We used 455 individuals, representing nine...

Background: The patterns of inbreeding coeficient (FIS) and fine-spatial genetic structure have been evaluated regarding mating system and inbreeding depression of food-deceptive orchid populations, Dactylorhiza majalis, Dactylorhiza incarnata var. incarnata, and Dactylorhiza fuchsii from NE Poland. Methods: We used 455 individuals representing nin...

A large suite of floral signals, and environmental and biotic characteristics influence the behavior of pollinators, affecting the female success of food‐deceptive orchids. In this study, we examined the many factors shaping the reproductive output of three orchid taxa: Dactylorhiza majalis , D. incarnata var. incarnata , and D. fuchsii . We applie...

Introduction Inbreeding depression (ID) in food-deceptive plants have been reported previously, however, it has not been often proven that selfed seeds germinate better than outbred ones or that selfing affects ID. To resolved these issues, food-deceptive related Dactylorhiza majalis, D. incarnata var. incarnata and D. fuchsii orchids were investig...

Carpinus betulus L., the hornbeam, is a component of lowland and highland forests in Europe. By examining the postglacial migratory history of thermophilic tree species, the study aimed to unravel their putative glacial microrefugia in the Carpathian region. The present study points to the two distinct genetic AFLP groups of C. betulus in the Carpa...

We are pleased to offer the book, which is the result of two-day virtual the 1st International Discussion Forum - Science in Practice: Wildlife Conservation in the Urban Environment. The forum was organized by the Faculty of Biology of the University of Bialystok, the Polish Society for the Protection of Birds and the City Hall in Bialystok and was...

A vast majority of angiosperms are pollinated by animals, and a decline in the number and diversity of insects often affects plant reproduction through pollen limitation. This phenomenon may be particularly severe in rare plant species, whose populations are shrinking. Here, we examined the variability in factors shaping reproductive success and po...

Opracowanie poświęcone jest problematyce kultury bezpieczeństwa oraz działającym w tym obszarze organizacjom pozarządowym. Mając na uwadze fakt, że źródłem sukcesu każdego przedsięwzięcia są ludzie i ich świadome zaangażowanie, uznano, że w procesie wdrażania zmian, takich jak kształtowanie odpowiednich postaw życiowych i moralnych, kluczowym dział...

Phylogenetic relations within Aconitum subgen. Aconitum (Ranunculaceae) in Europe are still unclear. To infer the phylogeny of the nuclear (ITS) region and chloroplast intergenic spacer trnL (UAG)-ndhF of the chloroplast DNA (cpDNA), we analyzed 64 accessions within this taxon, 58 from Europe and six from the Caucasus Mts. Nuclear ITS sequences wer...

Background and aims: The deception strategies of orchids remain poorly understood, especially in regard to the chemical compounds emitted from their flowers and their interaction with various taxonomic groups of pollinators. We investigated the phylogenetic relationships and compared the variation of floral chemical compounds between food-deceptiv...

Pollinator foraging behavior plays a key role in breeding and therefore affects the evolution of the orchid reproductive strategy. Food-deceptive orchids usually implement a generalized plant pollination strategy and a relatively diverse group of pollinators visit them. Dactylorhiza majalis is a food-deceptive, early-flowering orchid that relies on...

Deceptive plants represent a strategy that promotes outcrossing and protects against facilitated selfing. However, deception does not eliminate the possibility of autonomous self-pollination when pollinators are scarce or absent. Spontaneous autogamy is widespread in Orchidaceae, but the scale, mechanism, time of appearance and effectiveness remain...

Climate change is projected to influence the genetic resources of plant species. Recent research has examined genetic diversity patterns under current climate conditions, with little attention to the future genetic consequences for species. In this study, we combined ecological niche modeling and population genetic approaches to project future chan...

We carried out translocations of three rare plants that inhabit mineral islands in the marshy Biebrza Valley in order to create alternative populations and facilitate connectivity between existing subpopulations. The species chosen were Iris aphylla and two orchids: Cypripedium calceolus and Cephalanthera rubra. Thirty soil monoliths with vegetativ...

The deficiency of pollinators is indicated as the main factor limiting fruit set in orchids. Nectariferous species are more successful in setting fruits than nectar-less species. In the present study, we tested whether pollinator limitation lowers reproductive success in populations of Cypripedium calceolus on environmental islands in the Biebrza N...

Polemonium caeruleum (Polemoniaceae) represents a very interesting system of compatibility transition. Studies of its biological and ecological properties in the context of the breeding system of various populations may help to understand the evolutionary mechanism of this process. We investigated some aspects of the breeding system, diversity and...

Malaxis monophyllos is a rare orchid with a fragmented borealmontane distribution in Europe where it is associated with both natural swampy and anthropogenic habitats. We employed extensive sampling of M. monophyllos populations from different habitat types, over its whole European geographic range, to examine its genetic diversity patterns and phy...

The deficiency of pollinators is indicated as the main factor limiting fruit set in orchids. Nectariferous species are more successful in setting fruits than nectar-less species. In the present study, we tested whether pollinator limitation lowers reproductive success in populations of Cypripedium calceolus on environmental islands in the Biebrza N...

Malaxis monophyllos is a rare orchid with a fragmented boreal-montane distribution in Europe where it is associated with both natural swampy and anthropogenic habitats. We employed extensive sampling of M. monophyllos populations from different habitat types, over its whole European geographic range, to examine its genetic diversity patterns and ph...

Gene flow in plant populations is heavily affected by species sexual systems. In order to study the effect of sexual systems on genetic structure, we examined plastid and nuclear DNA of 12 dioecious (males and females) and 18 trioecious (males, females and hermaphrodites) populations of Salix myrsinifolia—a boreal shrub with slow range expansion. P...

Aconitum in Europe is represented by ca. 10% of the total number of species and the Carpathian Mts. are the center of the genus variability in the subcontinent. We studied the chloroplast DNA intergenic spacer trnL(UAG)-rpl32- ndhF (cpDNA) variability of the Aconitum subgen. Aconitum in the Carpathians: diploids (2n=16, sect. Cammarum), tetraploids...

The genetic diversity of two Aconitum species endemic to the Carpathian Mountains and Sudetes was studied. A reticulate evolution between them was earlier postulated as an effect of secondary contact. The genetic diversity at the individual and taxonomic levels was examined across the entire geographical ranges of the taxa in 11 populations based o...

The genetic diversity of two Aconitum species endemic to the Carpathian Mountains and Sudetes was studied. A reticulate evolution between them was earlier postulated as an effect of secondary contact. The genetic diversity at the individual and taxonomic levels was examined across the entire geographical ranges of the taxa in 11 populations based o...

We investigated the phylogeographical structure of the boreal-montane orchid Malaxis monophyllos in its Eurasian geographical range. We analysed four sequences of plastid DNA (trnL, trnL–trnF, rps16 and accD-psaI), resulting in 19 haplotypes and revealing a high level of intraspecific diversity (HD = 0.702 and π = 0.196 × 10−2), but showing a lack...

The aim of the paper was to test the hypotheses on the genetic structure and origins of forest herbaceous plant species, Galium schultesii and Stellaria holostea, in the subalpine zone of the Bieszczady Mts. The species are generally the lowland elements occurred in fertile oak-hornbeam forests. Their extrazonal occurrence in the mountain subalpine...

GC–MS was used to investigate the chemical diversity of bud exudates obtained from two species of white birch in Poland, Betula pendula Roth and Betula pubescens Ehrh. The ether extracts of the bud exudates contained more than 210 organic compounds including: terpenoids, phenylpropenoids of sesquiterpene alcohols and flavonoids. Headspace solid-pha...

Type of reproduction has an important effect on the maintenance of particular populations and species persistence in time and space. This trait significantly influences the ecological and genetic structure of populations, and in consequence the evolution of species. The primary objectives of this study were: to estimate genetic diversity within and...

A previous phylogeography and genetic diversity study of Chamaedaphne calyculata (Ericaceae) showed that populations over its geographic range were strongly separated into two groups: a Eurasian/NW North American group and a NE North American one corresponding with the disjunct distribution of Sphagnum-dominated peatlands in north-western and centr...

Isolated and island-like populations at the periphery of a geographic range of a given species are usually predicted to have low genetic diversity due to founder effect, habitat fragmentation, and bottleneck and/or inbreeding. As for parasitic plants, they may be more vulnerable to environmental and demographic stochasticities, habitat degradation,...

Surveys of genetic diversity patterns of self‐incompatible clonal polyploid plant species are still scarcer than those of diploid plant species. Therefore, I studied the phylogeographical history of L innaea borealis subsp. borealis to shed light on the colonization history of this clonal self‐incompatible polyploid plant in Eurasia using selected...

We examined the genetic diversity of two orchid species, the nectar‐rewarding Platanthera chlorantha and the nectarless Cephalanthera rubra, in north‐eastern Poland. We found lower differences in genetic diversity between the species than we expected. The level of genetic variation at species level was lower in C. rubra (P POL = 14%, A = 1.14 and H...

Using 32 allozyme loci, we examined the genetic diversity of ten populations of Neottia ovata differing in size and located in different regions of northeast Poland. Within-population genetic variation was low (Ppl = 9.4–31.2%, A = 1.09–1.34 and HE = 0.044–0.128) relative to taxa with similar life histories. In the majority of N. ovata populations...

In the last decade a number of studies has illustrated quite different phylogeographical patterns amongst plants with a northern present-day geographical distribution, spanning the entire circumboreal region and/or circumarctic region and southern mountains. These works, employing several marker systems, have brought to light the complex evolutiona...

In this work we assessed the genetic diversity of 32 C.calceolus populations from Poland. Mean genetic diversity was moderate (P=36.4%, A=1.58, H O=0.143, F IS=0.059), and seven geographic regions did not differ significantly in their levels of polymorphism (p>0.05), although allele frequencies varied greatly. Only four unique alleles were found, a...

The polyploid origin and colonization history of Iris aphylla in central and southeastern Europe were investigated by means of karyological and AFLP analyses. The study indicated two chromosome races within I. aphylla based on chromosomal numbers 2n = 24 and 2n = 48. The overall chromosome counts and determined ploidy suggest tetraploidy as the mos...

For evolutionary and ecological analyses, genetic diversity at different scales needs to be studied in terms of biological properties, habitat, population size and population history. We surveyed Platanthera bifolia populations from six regions in northeastern Poland to determine the impact of the mating system and population history on genetic div...

The patterns of genetic diversity in 14 Cypripedium calceolus populations were inves-tigated in two distant regions, located inside its continuous range (Biebrza valley, NE Poland) and close to the periphery of its range (Alps, SE France). The genetic variation at the species level was found to be relatively high (P% = 37.5%, H O = 0.145) as com-pa...

Kostrakiewicz, K. & Wróblewska, A. 2008: Low genetic variation in subpopulations of an endan-gered clonal plant Iris sibirica in southern Poland. — Ann. Bot. Fennici 45: 186–194. The spatial genetic structure in three subpopulations of the endangered clonal plant Iris sibirica from southern Poland was investigated. The subpopulations occurred in di...

The steppe plant Iris aphylla was chosen to describe the genetic diversity patterns and postglacial expansion over the whole geographical range. By studying 29 populations, both in the centre and at the periphery of the geographical range, a moderate level of genetic diversity (P % = 32.5%; H = 0.105), a low level of linkage disequilibria and a low...

Four Epipactis atrorubens (Hoffm.) Besser populations from Biebrza and Wigry National Parks were analyzed by starch gel electrophoresis to measure genetic and genotypic diversity. A total of 22 loci were analyzed, of which only two were polymorphic for the species. All populations had low levels of genetic variation (mean proportion of polymorphic...

Genetic diversity of Iris aphylla was examined in seven Polish populations located at the northern limit of the geographical range of the species. Using RAPD analysis, 491 DNA fragments were amplified, all of which were polymorphic. The peripheral populations were characterized by a low number of unique bands (one to three per population) and moder...

Enzyme electrophoreses were used to estimate genetic variation in five populations of Epipactis helleborine from two National Parks (Biebrza and Wigry) in northeast Poland. It has been proved that populations from these two regions differed in genetic structure, with the populations belonging to the Biebrza group having a higher level of genetic va...

Three populations of the orchid species Cephalanthera rubra localized on neighbouring mineral islands in the Biebrza National Park (north-east Poland) were examined using 16 allozyme loci. The percentage of polymorphic loci in these C. rubra populations ranged from 25 to 31.3% and the mean number of alleles per polymorphic locus from 1.25 to 1.31....

We used RAPDs (Random Amplified Polymorphic DNAs) to investigate genetic diversity and its partition within and between three populations of Iris aphylla in Poland. Analysis of Molecular Variance (AMOVA) of 84 distinct RAPD multiband genotypes revealed higher variation within populations (77.2%) than genetic differentiation between them (22.8%, P <...

Three populations of the rare and endangered plant species Cypripedium calceolus were included in a study of genetic diversity and spatial genetic structure in the Biebrza National Park, northeast Poland. Analysis of 11 allozyme loci indicate that the populations of this species contained high genetic variability (P = 45.5%, A= 1.73). On the other...

Two populations of the orchid species Listera ovata localised on mineral islands in the Biebrza National Park (NE Poland) were genetically examined with 32 allozyme loci. Genetic diversity was low (P = 9.4%; A = 1.09; Ho = 0.058). A moderate but significant genetic differentiation among the two populations was found (FST = 0.101). The observed low...

In the years 1997-2001 marginal population of Iris aphylla L. in the Biebrza National Park was investigated. The aims of study were to characterize population size, morphological traits, architecture, age structure of rhizomes and type of reproduction. During four years of observations fluctuations in numbers of rosettes (about 5-30%), significant...

The population genetic structure in the rare and endangered perennial plant, Cypripedium calceolus, from north-east Poland was investigated. A total of 11 loci were analysed, of which five were polymorphic for the species and in every population. The 11 loci resolved gave a total of 19 alleles with the mean number of 1.73 alleles per locus. The pro...

Популяции шести видов орхидных (Cypripedium calceolus, Listera ovata, Platanthera bifolia, Cephalanthera rubra, Epipactis helleborine и E. atrorubens) были изучены с использованием метода электрофореза. Генетической вариабельность была выявлена как между различными популяциями одного вида, так и между разными видами. Установлено, что C. calceolus и...