Achim E Gau

Achim E Gau
Leibniz Universität Hannover · Institute of Botany

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36
Publications
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694
Citations
Citations since 2016
0 Research Items
231 Citations
2016201720182019202020212022010203040
2016201720182019202020212022010203040
2016201720182019202020212022010203040
2016201720182019202020212022010203040

Publications

Publications (36)
Patent
Die vorliegende Anmeldung betrifft isolierte bifunktionale Polypeptide. Genauer betrifft die vorliegende Anmeldung Polypeptide mit einer Glucanaseaktivität und einer roteaseaktivität, wobei das Polypeptid ein Derivat eines natürlich vorkommenden Polypeptids mit Glucanase- und gleichzeitig einer Proteaseaktivität ist. In einem weiteren Aspekt werden...
Patent
Full-text available
Die vorliegende Anmeldung betrifft isolierte bifunktionale Polypeptide. Genauer betrifft die vorliegende Anmeldung Polypeptide mit einer Glucanaseaktivität und einer roteaseaktivität, wobei das Polypeptid ein Derivat eines natürlich vorkommenden Polypeptids mit Glucanase- und gleichzeitig einer Proteaseaktivität ist. In einem weiteren Aspekt werden...
Article
With 3 figures and 5 tables Grain legumes are regarded as highly valuable protein source for human and animal nutrition. Legume protein quality is mainly limited by the amino acids (AAs) tryptophan and sulphur AA. Organic farming in particular requires high seed protein quality for livestock feeding, as chemically produced AAs must not be feeded. B...
Article
Full-text available
The protein encoded by the FLOWERING LOCUS T (FT) gene from Arabidopsis thaliana seems to be the long-searched florigen, and over-expression of FT orthologues resulted in accelerated flower development in annual and perennial plants. In the present study, we isolated two allelic mRNA sequences of an FT-homologous gene from apple, which was designat...
Article
Full-text available
One of the major problems in feeding organic pigs and poultry with 100 % organic feedstuff is the insufficient methionine (Met) content in the protein of local European grain legumes. The aim of the presented work is to find practicable and cheap indicators to select breeding can- didates for growing legume cultivars with high seed-Met content unde...
Article
Full-text available
Transgenic apple plants (Malus x domestica cv. 'Holsteiner Cox') overexpressing the Leaf Colour (Lc) gene from maize (Zea mays) exhibit strongly increased production of anthocyanins and flavan-3-ols (catechins, proanthocyanidins). Greenhouse plants investigated in this study exhibit altered phenotypes with regard to growth habit and resistance trai...
Article
Full-text available
Meristems in apple have to pass the vegetative and the reproductive phase change before they can produce flowers. In the previous ten years several genes of apple were identified which are homologous/orthologous to flowering genes of Arabidopsis thaliana. In relation to results obtained in Arabidopsis, the apple genes MdCOL1, MdCOL2, MdFT, MdSOC1,...
Article
The present study investigated the inhibitory effect on the conidial germination of Venturia inaequalis by using living whole cells, extracellular protein fraction and individual proteins from the non-pathogenic antagonistic bacterium Pseudomonas fluorescens Bk3.The bacterial suspension of P. fluorescens Bk3 from growth in minimal medium showed up...
Article
A plant lectin was isolated from barley (Hordeum vulgare) coleoptiles using acidic extraction and different chromatographic methods. Sequencing of more than 50% of the protein sequence by Edman degradation confirmed a full-length cDNA clone. The subsequently identified open reading frame encodes for a 15 kDa protein which could be found in the solu...
Article
Full-text available
The two closely related fresh water cyanobacteria Synechococcus elongatus PCC 6301 and Synechococcus elongatus PCC 7942 have previously been shown to constitutively express a FAD-containing L-amino acid oxidase with high specificity for basic L-amino acids (L-arginine being the best substrate). In this paper we show that such an enzyme is also pres...
Article
Full-text available
Biological control of plant diseases by the application of antagonistic micro-organisms to the plant phyllosphere is only marginally understood. Suppression subtractive hybridization (SSH) was used for the identification of genes expressed after application of the non-pathogenic bacterium Pseudomonas fluorescens Bk3 to the phyllosphere of the apple...
Article
In order to compare transcription profiles in cultivars of Malus domestica that are differentially sensitive to apple scab (Venturia inaequalis), two cDNA libraries were constructed using the suppression subtractive hybridization (SSH) method. Subtraction hybridization was performed between cDNAs from uninfected young leaves of the resistant cultiv...
Article
Full-text available
The fungus Venturia inaequalis clone No. 36 isolated from Malus domestica cv. Gloster excretes a melanoprotein of 36 kDa in relatively high amounts during growth in liquid culture. The protein was isolated from the culture medium and purified to homogeneity. It was shown to contain melanin. After raising an antiserum against the isolated protein, t...
Article
In order to understand better the survival and mutual interaction of epiphytic bacteria and fungi on apple plants, bacteria collected from these plants were cultivated on intact adaxial, stoma free cuticle membranes originally obtained from apple. The bacteria were labelled with luciferase genes from Vibrio harveyi in order to follow up their devel...
Article
Full-text available
The intercellular washing fluid (IWF) of Malus domestica cv. Holsteiner Cox before and after application of the non-pathogenic bacterium Pseudomonas fluorescens Bk3 to the leaves was investigated in a comparative manner. SDS-PAGE in combination with ESI Q-ToF mass spectrometry, and homology search in relevant data bases revealed the highly up-regul...
Article
Full-text available
Leaves of apple (Malus domestica cv. Elstar) were infected with a cloned isolate of the apple scab Venturia inaequalis. The intercellular washing fluid (IWF) of these plants was collected and the variation in the composition of proteins in the IWF was analysed by SDS-PAGE and two-dimensional gel electrophoresis during and after the infection with V...
Article
Epiphytic living Pseudomonas strains isolated from different Malus domestica cultivars were transformed with two reporter genes [green fluorescent protein (gfp) and luciferase (luxAB)]. The establishment and distribution of these bacteria on sterile, in vitro-propagated, and thus genetically identical, Malus domestica plants were continuously analy...
Article
We describe two related manganese-binding polypeptides with L-arginine metabolizing enzyme activity that can be detected as distinct components (designated PsbY-A1 and PsbY-A2, previously called L-AME) in membranes containing Photosystem II (PS II) from spinach. The polypeptides are bitopic and appear to exist in a heterodimeric form, but only in t...
Article
Full-text available
A low ʟ-arginine metabolizing enzyme (L-AME) activity leading to ornithine, urea and additional products not identified so far could be detected in photosystem II (PS II) membranes of spinach and of the chlorophyll deficient tobacco mutant Su/su. The detectable L-AME activity was very low in untreated PS II membranes, but increased significantly (a...
Article
Photosystem II complexes from the cyanobacterium Anacystis nidulans have been investigated by Western blots with antisera raised against four photosystem II peptides from plants and with an antiserum raised against the soluble L-amino acid oxidase protein from A. nidulans to achieve an identification of the polypeptides — especially of the L-amino...
Article
Full-text available
The absorption spectrum of the previously purified L-amino acid oxidase from the cyanobacterium Anacystis nidulans has shown considerable variation with each preparation and the spectrum in several preparations was quite different from the absorption spectrum of other simple flavoproteins (E. K. Pistorius and A. E. Gau, Biochim. Biophys. Acta 849,...
Article
O2-evolving photosystem II complexes, which were isolated from the cyanobacterium Anacystis nidulans as described previously [(1986) Biochim. Biophys. Acta 849, 203-210], were further purified on a Sepharose 6B column. These complexes retain the L-amino acid oxidase protein. Moreover, various types of photosystem II inhibitors were tested for their...
Article
In the presence of K02, the naturally occurring N-acylamines (I) are cleaved to give the amides (II) (conversions: 36-53%).
Article
A highly active O2-evolving Photosystem II complex which was greatly depleted of phycobiliproteins was isolated from the cyanobacterium Anacystis nidulans. This complex contained the flavoprotein with l-amino acid oxidase activity which we have previously shown to be present in thylakoid preparations of this cyanobacterium (Pistorius, E.K. and Voss...
Article
Full-text available
N-Acyl-glycine derivatives are cleaved by the action of potassium superoxide in ether or pyridine solution to give the corresponding carboxamides.
Article
Full-text available
The biological control of plant diseases by application of antagonistic microorganisms to the plant phyllosphere is an alternative strategy to prevent the frequent treatment of plants by pesticides. Microbiological antagonists can firstly interact directly against the pathogen by releasing antimicrobial compounds and/or secondly induce the plant re...
Article
Full-text available
Grain legumes possess a generally favour-able seed protein amino acid composition but low contents of sulfur amino acids de-crease their nutrient value. To enhance the methionine content of local grain legumes by plant breeding, phenotypical attributes for high methionine contents are required for the selection process. The efforts are of particula...

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