Achariya Sailasuta

Achariya Sailasuta
Chulalongkorn University · Department of Pathology

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15
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Publications

Publications (15)
Article
Full-text available
Canine cutaneous mast cell tumors (MCT) are the lethal skin tumors. The biological behavior of the MCT cells is quite varied and unpredictable. Almost MCT dogs usually require a rapid diagnosis and therapy. However, MCT diagnosis and prognosis are still dependent on histopathology which is rather inconvenient, time-consuming, painful, and harmful f...
Article
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The pathological alterations in skin keratinocyte proliferation and differentiation in canine atopic dermatitis (CAD) were investigated in 20 small breed dogs with CAD (10 of which displayed lesional CAD skin and 10 nonlesional CAD skin) and 11 healthy control animals. Biopsy of lesional CAD skin showed orthokeratotic hyperkeratosis. Immunohistoche...
Article
Canine atopic dermatitis (CAD) is a common allergic skin disease in dogs, associated with a defective epidermal barrier. In this study we investigated the alterations in skin keratinocyte proliferation and differentiation in CAD by quantitative reverse transcription-polymerase chain reaction. Gene expression of keratin (KRT) markers of proliferativ...
Article
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The purpose of this study was to measure the thickness of canine epidermis at various anatomical sites according to localization of cornified envelopes (involucrin and filaggrin), keratins (keratin 10, 5), and their mRNA expression. This was done in the skin of five breeds of dogs including seven poodles, six golden retrievers, six Shih Tzus, four...
Article
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As a cornified envelope protein, filaggrin (FLG) is involved in the formation and support of the skin barrier. FLG single nucleotide polymorphism (SNP) at the position 64,297,022 (rs22588227) on canine chromosome 17 has initially been described as being associated with canine atopic dermatitis (CAD) in Labrador Retrievers in UK. In this study, we h...
Article
Yellow-headed temple turtles (YHT), Hieremys annandalii, native to Thailand, are protected from exploitation under the Wild Animal Reservation and Protection Act, also listed under Appendix II of the Convention on International Trade of Endangered Species and the International Union for the Conservation of Nature red list. The objectives of this st...
Article
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One hundred and forty adult rice field frogs, Hoplobatrachus rugulosus (Wiegmann, 1834), were collected in Srakaew province, Thailand. For blood parasite examination, thin blood smears were made and routinely stained with Giemsa. The results showed that 70% of the frogs (98/140) were infected with 5 species of blood parasites, including a Trypanoso...
Article
The objective of this work was to explore the potential and safety of trimethyl chitosan (TMC) and PEGylated TMC for improved absorption of insulin after nasal administration. The nasal absorption of insulin nanocomplexes of TMC or PEGylated TMC was evaluated in anaesthetized rats. Concomitantly, the histopathological effects of these nanocomplexes...
Article
Full-text available
Helicobacter pylori infection has been known to associate with gastric and peptic ulcer, mucosa-associated lymphoid tissue, lymphoma and gastric carcinoma. The hyperimmune serum prepared from six New Zealand White rabbits, by immunizing four times, biweekly with Helicobacter pylori bacterial extracts were used in this assay. The antisera were colle...
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Detection of antibodies against Helicobacter pylori by indirect immunofluorescent assay (IFA) in rabbits has been developed as a non-invasive screening method. This study aimed to detect the antibody response by IFA after immunization with Helicobacter pylori in rabbits. Six healthy New Zealand white female rabbits were immunized subcutaneously wit...
Article
Full-text available
INTRODUCTION Over the last decades, intranasal delivery has been widely investigated as an alternative route for peptide and protein drugs such as insulin. Amongst several strategies used for enhancement of the insulin absorption, the use of colloidal polymeric particulate delivery systems represents a promising concept. Due to amphoteric nature of...
Article
Objective: To evaluate the safety and efficacy of chitosan (CS) as nasal absorption enhances of peptides. Methods: Two types of chitosans, i.e. CS J (free amine chitosan) and CD G (water-soluble glutamate salt), were evaluate for their masal absorption enhancing effectd on L-Tyr-D-Arg([D-Arg[square]]-Kyotorphic), an enzymatically stable dipeptide,...