Abraham Peele- Doctor of Philosophy
- Professor (Assistant) at Vignans foundation for science technology and research
Abraham Peele
- Doctor of Philosophy
- Professor (Assistant) at Vignans foundation for science technology and research
About
68
Publications
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Introduction
Abraham Peele currently works at the School of Biotechnology, Vignan University. Abraham does research in Microbiology.
Skills and Expertise
Current institution
Vignans foundation for science technology and research
Current position
- Professor (Assistant)
Publications
Publications (68)
Purpose
Alzheimer’s disease (AD), the most common cause of dementia, is a neurodegenerative disorder caused by the aggregation of amyloid-beta (Aβ) at outside of neuron cells and also due to tau aggregation inside the cell. Corosolic acid is aimed to be selected as a main active constituent of Lagerstroemia speciosa for the study.
Design/methodolo...
Pulmonary fibrosis is characterized by damage to the epithelial cells and alveolar-capillary basement membrane. The increased expression levels of transforming growth factor β (TGF-β) and TGF-β-receptor-1 induced differentiation of lung fibroblasts to myofibroblasts, an alarming sign and considered the hallmark event development of pulmonary fibros...
A biocompatible material for drug delivery remains challenging for decades. Exopolysaccharides (EPSs) produced by marine bacteria have profound impact in biomedical, biotechnological and pharmaceutical applications in nano-scale. EPSs are heterogeneous polymers that can be used as biocompatible materials to coat and stabilize nanoparticles for the...
Background
Extracellular polysaccharides (ECPs) produced by biofilm-producing marine bacterium have great applications in biotechnology, pharmaceutical, food engineering, bioremediation, and bio-hydrometallurgy industries. The ECP-producing strain was identified as Acinetobacter indicus M6 species by 16S rDNA analysis. The polymer produced by the i...
Bioprospecting of natural molecules for the development of medicines, nutraceuticals, and bioactive leads has gained attention in the industrial market. The bioactive compounds from microorganisms have huge commercial demand in various industries. The immense biodiversity of microorganisms has remained unexplored from extreme environments, which is...
The coronavirus disease (COVID-19) pandemic has caused havoc worldwide. The tests currently used to diagnose COVID-19 are based on real time reverse transcription polymerase chain reaction (RT-PCR), computed tomography medical imaging techniques and immunoassays. It takes 2 days to obtain results from the RT-PCR test and also shortage of test kits...
The current study presents chromium (VI) biosorption performance of Spongomorpha indica from synthetic medium. Spongomorpha indica, naturally available green marine alga was selected as an adsorbent. The influence of three process parameters i.e., initial pH, initial chromium (VI) ions concentration and Spongomorpha indica biomass dosage, on the pe...
Laccases are the green tools that can find potential applications in various industries. There are many reports available on laccases from plants and fungal sources but very few reports are available on bacterial laccases. Bacterial laccases show broad range of substrate specificity and it is easy to isolate and purify the bacterial extracellular l...
The discipline of nanotechnology has gained momentum in the agriculture field and emerged to modernize the research related to agricultural practices. The use of a lot of chemicals in the agriculture field led to environment pollution, climate change, diminishing natural resources, and other ecological impacts. In recent years, nanotechnology gaine...
The novel coronavirus (SARS-CoV-2) outbreak has started taking away the millions of lives worldwide. Identification of known and approved drugs against novel coronavirus disease (COVID-19) seems to be an urgent need for the repurposing of the existing drugs. So, here we examined a safe strategy of using approved drugs of SuperDRUG2 database against...
Hydroponics is a cultivation of plants without soil with formulated nutrient medium growing in a fabricated set of unit. The present study focused on the nine different medicinal and ornamental plants. They were grown in the hydroponics unit supplied with macro and micro nutrients of three different treatments ranges from 200ppm to 450ppm. Experime...
In the present study, one of the targets present on the envelopes of coronaviruses, membrane glycoprotein (M) was chosen for the design of a multi-epitope vaccine by Immunoinformatics approach. The B-cell and T-cell epitopes used for the construction of vaccine were antigenic, nonallergic and nontoxic. An adjuvant, β-defensin and PADRE sequence wer...
C RISPR technology originated from repetitive nucleic acid sequences discovered in bacterial DNA. Among these sequences viral genome particles were found. These particles are used by CRISPR associated proteins to target the invading viral genome. [1, 2] Cas proteins have the ability of cleaving viral nucleic acids when activated by complementary ba...
Coronavirus disease 2019 (COVID-19), a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has drastically changed the lifestyle of people around the globe. Due to the lack of specific and effective antiviral drugs, transmission of the disease increases exponentially and makes it more serious and harder...
In the present study, we explored phytochemical constituents of Tinospora cordifolia in terms of its binding affinity targeting the active site pocket of the main protease (3CL pro) of SARS-CoV-2 using molecular docking study and assessed the stability of top docking complex of tinosponone and 3CL pro using molecular dynamics simulations with GROMA...
In the present study, biosorption of lead using green algae as biosorbent from aqueous solution was studied with one-factor-at-a-time (OFAT) method. The batch biosorption process is a function of agitation time, biomass loading, initial concentration of lead and solution pH. Further, face-centered central composite design under RSM application was...
Breast cancer is one of the serious health issues for the women and it has been characterized with
uncontrolled growth of cells in the breast tissue. An elevated level of expression of estrogen promotes
survival to breast cancerous cells. The present study is focused on phytochemicals, to screen the
inhibition of estrogen receptor activity. The sel...
The best therapeutic strategy to find an effective vaccine against SARS-CoV-2 is to explore the target structural protein. In the present study, a novel multi-epitope vaccine is designed using in silico tools that potentially trigger both CD4 and CD8 T-cell immune responses against the novel Coronavirus. The vaccine candidate was designed using B a...
The aim of this study was to develop an appropriate anti-viral drug against the SARS-CoV-2 virus. An immediately qualifying strategy would be to use existing powerful drugs from various virus treatments. The strategy in virtual screening of antiviral databases for possible therapeutic effect would be to identify promising drug molecules, as there i...
Biodiesel has intrinsic lubricating properties due to the presence of long chain fatty acids, which significantly help to reduce engine wear and tear. Poultry waste as an alternative energy source could aid in reducing greenhouse gases. Biodiesel acknowledges environmental issues as it is less toxic than diesel and is biodegradable too. It is much...
Mangroves contain a wide range of bioactive compounds with pharmacological activities. In the present study, we analysed the separation and detection of phytoconstituents with the methanol extract of Ipomoea tuba leaf using gas chromatography-mass spectrometry (GC-MS) and tested its in vitro cytotoxicity effect against MCF-7 and HeLa cells. Phytoch...
Saccharomyces boulardii is used for antimicrobial peptide production and so can be termed as
bacteriocin molecules with probiotic potential. Antimicrobial peptides are extracted from the culture broth
and followed the purification using Sephadex G-50 column. Further, the inhibition activity was determined
against selected microorganisms and inhi...
Background
ATP-binding cassette (ABC) transporters constitute one of the largest transporter protein families and play a role in diverse biological processes.
Results
In the present study, bacteriocin isolated from the Enterococcus casseliflavus MI001 strain was identified as an ABC transporter ATP-binding protein. The optimal conditions for the p...
In the present study, the microbial source for potent lactase producers was explored to supplement the lactase intolerant individuals. Dairy effluent was screened for lactase producing bacteria by conventional microbiological methods. Among the positive isolates, one isolate VUVD001 was found to be a strong producer of lactase enzyme and this strai...
In the present study, biosorption capacity of Tamarindus indica is investigated for removal of lead and zinc. The heavy metals, namely lead and zinc, are successfully removed from aqueous solution by batch biosorption using Tamarindus indica as biosorbent. The removal percentage of selected metals, namely lead and zinc, was improved through optimiz...
The bacteria residing in the gut environment do play a pivotal role in metabolic activities of the host. The metabolites produced by these bacteria affect the physiology and health of the host. The gut bacteria are exposed to environmental conditions where multiple factors such as lifestyle, stress, antibiotics, host genetics and infections have an...
In the present study, phytochemical and antimicrobial analysis of selected mangrove species: Suaeda nudiflora, Lumnitzera racemosa, Ipomoea tuba and Avicennia alba was performed. The phenol found in all selected mangroves and tannins found in the species: Suaeda nudiflora, Ipomoea tuba and Avicennia alba and terpenoids present in Lumnitzera racemos...
The aim of present study is to analyze and separate bioactive constitutes of methanol leaf extract from Lumnitzera racemosa by Gas chromatography–Mass spectroscopy (GC–MS) and further to test for in vitro anticancer activity on MCF 7 and HeLa cancer cells using MTT assay. GC–MS analysis of L. racemosa leaf extract showed the presence of different b...
The current study focused on GC-MS analysis for identification of bioactive compounds in leaf extract of mangrove plant, Avicennia alba and the leaf extract of Avicennia alba was tested for cytotoxicity against MCF 7 and HeLa cell lines. The spectral properties (GC-MS) of each separated compound were determined and found the different compounds nam...
Eco-friendly biopolymers, polyhydroxybutyrate, have been produced by many kinds of bacteria that possess most important applications in food packaging industries and also in medical field. In the present work, PHB production was economized through the statistical optimization of nutritional components. The preferable carbon and nitrogen sources cho...
The discovery of antimicrobial bacteriocin molecules with broad-spectrum activity against bacterial infections caused by spoilage microorganisms is vital for food safety. In this study, a potential bacteriocin-producing bacterium was isolated, screened and confirmed as Enterococcus casseliflavus MI001 by 16s rDNA sequencing. The bacteriocin peptide...
In the present investigation, a low-cost fermentation medium was designed to achieve the maximum productionof lactase from theBacillussp. VUVD101 strain through the screening of different nutritional and physicalvariables, using the Plackett-Burman design. Fourteen variables of the fermentation process were selected: in-cubation time, temperature,...
A novel approach of cellulase-based co-culture producing bioethanol using low-cost nutrient medium has been employed for the study, and docking strategies provided the information of cellobiose and cellotetraose inhibitors during cellulase production. The Acinetobacter species was isolated from termite gut and confirmed in 16s rDNA analysis. SDS-PA...
Background: Homeostasis of blood glucose concentration is a physiological phenomenon that is tightly regulated in humans and acts as a major cellular mechanism that decreases blood glucose during insulin-stimulated glucose transport. GLUT4 assumes a significant role in managing entire body glucose homeostasis. Recent research has found that overexp...
Biosurfactants are amphiphilic compounds produced by bacteria either extracellularly or as a part of the cell membrane. Biosurfactants have had a profound impact on medical and pharmaceutical biotechnology. In our previous work, we isolated a new biosurfactant produced by Acinetobacter indicus M6 which reduces the surface tension of water from 72.0...
CRISPR genome editing utilizes Cas9 nuclease and single guide RNA (sgRNA), which directs the nuclease to a specific site in the genome and makes a double-stranded break (DSB). Design of sgRNA for CRISPR-Cas targeting, and to promote CRISPR adaptation, uses a regulatory mechanism that ensures maximum CRISPR-Cas9 system functions when a bacterial pop...
The energy resources mainly petroleum and petroleum hydrocarbons are major pollutants of the environment. The oil and oil products contamination may cause severe harm and hence, the attention has been remunerated in the development of alternative technologies for elimination of these contaminants. Biosurfactants were used in the remediation of oil...
Acne vulgaris (acne) is a chronic inflammatory disease prevalent among adolescents and adults, with significant psychological effects. The aetiology of acne is multifactorial. Several pathophysiological associations have been identified in which Propionibacterium acnes plays a major role. This bacteria primarily affects areas containing oil glands...
Biosurfactants produced by biofilm-forming bacteria have great applications in biotechnology, pharmaceutical, food engineering, bioremediation, and biohydrometallurgy industries. This study aimed to find out the bacteria that produce novel exopolymers (EPSs) which can find potential role in oil biodegradation. A screening procedure was performed to...
Quorum sensing mechanisms open the lock of the unexplored bacterial world with its molecular key by cracking the codes of gene programs in bacteria. Bacteria uses quorum sensing AHL molecules to build an Exopolysaccharaide matrix coat which heals the cracks in concrete. Specimens were prepared at 105 cells/ml concentration for concrete grade of M30...
Bacteria produces many kinds of molecules that allow bacteria to communicate about population size, metabolic states or producing end products that initiate some activities such as bioluminescence. These signal molecules are generally called as autoinducers. Quorum sensing reveals the fact that bacteria have the capacity to assess the number of oth...
Biofuels are the major contributors of energy sources and an alternative fuels to fossil fuels. As the vegetable derived oils are
becoming scarce even to human needs, we definitely have the need to find the alternative routes. Chicken feather meal consists
of processed chicken feathers which has an approximate of 12% fat content, which could be use...
Poly hydroxyl butyrate (PHB) can be used as an effective thermoplastic, and has many characteristics similar to those of standard commercial plastics like polypropylene. PHB-based plastic substitutes are less flexible than traditional plastics, they are completely biodegradable and leave behind no residue. In this present study high PHB producing s...
Concrete is one of the widely used construction materials. It has a variety of disadvantages that should be considered before use. One main disadvantage of concrete is that all structures made from it will crack at some point. Concrete can also crack as a result of shrinkage, which happens when it dries out. These cracks develop within a few days o...
Plastics are typically polymers of high molecular weight and one of the chemical materials which cause severe environmental problems. Improper disposal of plastic bags blocks the porosity of the soil and causes problems for groundwater recharge. Due to its non-biodegradable nature, littering of plastics causes irreversible damage to the environment...
Bacteriocins are proteinaceous compounds, which are ribosomally synthesized antimicrobial peptides produced by both Gram-positive and Gram-negative bacteria. They are phenomenologically analogous to yeast and paramecium killing factors, and are structurally, functionally, and ecologically diverse. Bacteriocins differ from the traditional antibiotic...
Antimicrobial agents have been used for the treatment of different microbial infections on human health. Biofilm producing bacteria exist in biofilm and with stand harsh environmental conditions and antimicrobial agents. Most microbial infections caused by biofilm producing bacteria are resistance to conventional therapy. Researchers have been prom...
Environment friendly biodegradable plastics are gaining popularity in the recent years because of the burning needs that facing the society. Natural polymers that use polysaccharides such as starch and microbial Exopolysaccharaides blends with accelerated degradation properties. Biodegradable plastic offers promise to solve the problem of the dispo...
Microbial biofilms cause a variety of microbial infections on human health, such as urinary tract infections, catheter infections, middle-ear infections, formation of dental plaques, gingivitis, coating contact lenses. In the present study, the high yielding biofilm strains have been isolated from the soil and characterized. Microtitre plate assay...
Questions
Questions (15)
We have screened the phytochemicals of Tinospora cardifolia and curcuma longa downloaded from Imppat database and Dr. Dukes database and targeted TGFbeta-R1 crystal structure using docking study and found one best compound from each quercetin from c.longa and tembetarine from T.cardifolia , Inorder ro test the compound to test invitro & invivo, we wish to purchase from chemical vendors but we found the compounds source from other plant species (Apis mellifera) but structure remains same for both cases. Is it a valid study if we conduct a study based on this
link : Quercetin ≥95% (HPLC), solid | 117-39-5 (sigmaaldrich.com)
We have screened the phytochemicals of Tinospora cardifolia and curcuma longa downloaded from Imppat database and Dr. Dukes database and targeted TGFbeta-R1 crystal structure using docking study and found one best compound from each quercetin from c.longa and tembetarine from T.cardifolia , Inorder ro test the compound to test invitro & invivo, we wish to purchase from chemical vendors but we found the compounds source from other plant species (Apis mellifera) but structure remains same for both cases. Is it a valid study if we conduct a study based on this
link : Quercetin ≥95% (HPLC), solid | 117-39-5 (sigmaaldrich.com)
Dear Respected Sir/madam
i want to find the conserved sequences of spike protein gene or rbd domain gene to design CRISPR single guide RNA and the design of sequence should be unique to identify the PAM sequences in CHOP CHOP tool... could you please suggest me the work flow
From the MSA alignment of covid-19 genes (spike or rbd) how much length of conserved sequences can be identified.
These stretches of conserved sequences can be blasted against other viral genomes and sequences unique to covid-19 can be identified ??
which tools i have to use ??
Dear Respected Sir/madam
i want to find the conserved sequences of spike protein gene or rbd domain gene to design CRISPR single guide RNA and the design of sequence should be unique to identify the PAM sequences in CHOP CHOP tool... could you please suggest me the work flow
From the MSA alignment of covid-19 genes (spike or rbd) how much length of conserved sequences can be identified.
These stretches of conserved sequences can be blasted against other viral genomes and sequences unique to covid-19 can be identified ??
which tools i have to use ??
If i take transfused blood from some donor .. do my cells contain two different types of DNA, As White blood cells from the donor contains DNA of his own, After Blood transfusion, do i contain two different persons DNA in my blood??
I would like to produce lactic acid in my laboratory. Can anyone please tell me the procedure, aerobic or anaerobic conditions to maintain. After growing culture in defined media. How to extract the lactic acid? And how to quantitatively estimate the lactic acid produced?
How many grams of diphenylcarbazide should be taken to dissolve in 95% ethyl alcohol?
We isolated a pure strain of an unknown bacteria we would like to check what enzymes its been producing
i hv grown lactobacillus acidophilus in MRS broth amd after 24 hrs i extracted the supernatant by centrifugation at 11000 rpm for 10 min ... with out checking any ph i checked bacteriocin activity of crude supernatant on test strains like e.coli . strepto . staphylo , kliebsella ... it is not showing any inhibition zones ... could any one suggest any modificationsss
i hv grown lactobacillus acidophilus in MRS broth amd after 24 hrs i extracted the supernatant by centrifugation at 11000 rpm for 10 min ... with out checking any ph i checked bacteriocin activity of crude supernatant on test strains like e.coli . strepto . staphylo , kliebsella ... it is not showing any inhibition zones ... could any one suggest any modificationsss
Some review papers give tannin broth has containing yeast extract, sucrose, nacl and tannin. My problem is I have tannic acid instead of tannin in my lab. When I use tannic acid, is is solified like agar. What should I do?
