Abinet Legesse

Abinet Legesse
  • Microbiology
  • Researcher at National Veterinary Institute, Ethiopia

camel brucellosis, Animals immunization, sera collection, serological tests, Nucleic acid extraction, molecular works.

About

20
Publications
6,223
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83
Citations
Introduction
Abinet Legesse holds an MSc degree from Addis Ababa University. He currently works at the National Veterinary Institute, Ethiopia, as a senior researcher on Vaccine research and development. He has ample experience in animal immunization, serological tests, and molecular works. His most recent publication is 'Isolation and Genetic Characterization of Genotype VII Velogenic Pathotype Newcastle Disease Virus from Commercial Chicken Farms in Cent
Current institution
National Veterinary Institute, Ethiopia
Current position
  • Researcher
Additional affiliations
March 2019 - September 2024
National Veterinary Institute, Ethiopia
Position
  • senior researcher
Description
  • Ovine mannheimosis, ruminant brucellosis, camel brucellosis, Animal immunization, serological tests, infectious Diseases, ISO 17025 signatory, Virology, nucleic acid extraction, PCR works, and gel-electrophoresis.
Education
July 2014 - July 2017
Addis Ababa University college of agriculture and veterinary medicine
Field of study
  • Microbiology

Publications

Publications (20)
Article
Full-text available
Infectious bursal disease is a highly contagious disease of young chickens caused by the infectious bursal disease virus. This disease poses an important threat to the commercial poultry industry globally. This study was designed to develop an In-House Indirect Enzyme-Linked Immune Sorbent Assay Kit for the serological detection of antibodies again...
Article
Full-text available
Background Avian infectious bronchitis (IB) is a highly contagious respiratory disease that affects the poultry industry globally. The disease is caused by avian infectious bronchitis virus (IBV), member of the genus Gammacoronavirus. In Ethiopia, IBV has been reported in both commercial and backyard chickens based on clinical observation. The obje...
Article
Full-text available
Background Avibacterium paragallinarum is a causative agent of infectious coryza (IC), a disease that affects the upper respiratory tracts and paranasal sinuses of chickens, resulting significant economic losses in the poultry industry. The objective of this study was to isolate and identify Av. paragallinarum using bacteriological and molecular me...
Article
Full-text available
Introduction Infectious laryngotracheitis (ILT) is a highly contagious upper respiratory tract disease of chickens caused by a Gallid herpesvirus 1 (GaHV-1). The current study was to establish molecular evidence of Infectious laryngotracheitis virus (ILTV) in the Amhara region, Ethiopia, and determine its seroprevalence in areas of high chicken pop...
Article
Full-text available
Background: Infectious diseases such as Peste des Petits Ruminants, Contagious Caprine Pleuropneumonia, Sheep and Goat Pox, and Pasteurellosis have considerable impacts on the optimal utilization of sheep and goat resources in Ethiopia. Immunization using multiple vaccines administered simultaneously has been suggested as a cost-effective and safe...
Article
Full-text available
Newcastle disease (ND) is caused by virulent strains of avian paramyxovirus type 1, also known as Newcastle disease virus (NDV). Despite vaccination, the frequency of reported outbreaks in Ethiopia has increased. From January to June 2022, an active outbreak investigation was conducted in six commercial chicken farms across areas of central Ethiopi...
Article
Full-text available
Coronaviruses are enveloped, large positive-sense, and single-stranded RNA viruses. Four coronavirus genera have been identified: alpha, beta, gamma, and delta. Non-structural proteins make up two-thirds of the coronavirus genome, and some of these (ORF1ab) are essential to create the replicase-transcriptase complex. The remaining coronavirus genom...
Article
Full-text available
Background Pasteurella multocida is a bacterial pathogen that causes a variety of infections across diverse animal species, with one of the most devastating associated diseases being hemorrhagic septicemia. Outbreaks of hemorrhagic septicemia in cattle and buffaloes are marked by rapid progression and high mortality. These infections have particula...
Article
Full-text available
Pasteurellaceae families are usually considered opportunistic pathogens which inhabit normal flora on the mucosal membranes of the upper respiratory and the lower genital tracts of mammals and birds. The majority of P. multocida, M. hemolytica, and B. trehalosi isolates are opportunistic animal pathogens and cause disease only under certain conditi...
Preprint
Full-text available
Infectious Bursal Disease Virus (IBDV) causes a highly contagious disease in young chickens. Due to its worldwide distribution, IBDV poses an important threat to the commercial poultry industry. Lymphoid cells in the bursa of Fabricius are the target cells of IBDV serotype-1 strains. The study was designed for the development of an In-House Indirec...
Preprint
Full-text available
BACKGROUND ANDMETHODS: Brucellosis is a dreadful zoonotic disease affecting humans and all domestic animals including camels worldwide. Serological evidence for Brucella infection in camels has been reported from all pastoralist and agro-pastoralist regions of Ethiopia. Investigations have shown that antibody concentrations are lower in camels than...
Preprint
Full-text available
BACKGROUND ANDMETHODS: Brucellosis is a dreadful zoonotic disease affecting humans and all domestic animals including camels worldwide. Serological evidence for Brucella infection in camels has been reported from all pastoralist and agro-pastoralist regions of Ethiopia. Investigations have shown that antibody concentrations are lower in camels than...
Article
Full-text available
Infectious bronchitis (IB) is a highly contagious disease of the respiratory and urogenital tract of chickens, caused by infectious bronchitis virus (IBV), a member of the family Coronaviridae. Due to the serious infectious and transmission features of the disease mostly in the reproductive and respiratory systems it causes potential economic loss....
Article
Full-text available
Background Brucellosis is an economically devastating animal disease and has public health concern. Serological methods such as Rose Bengal Plate Test (RBPT), Complement Fixation Test (CFT), and Indirect-Enzyme-Linked Immunosorbent Assay (I-ELISA) have been used to detect brucellosis. However, there is limited comparative evaluation studies and lac...
Article
Full-text available
Pasteurella multocida can infect a multitude of wild and domesticated animals, with infections in cattle resulting in hemorrhagic septicemia (HS) or contributing to bovine respiratory disease (BRD) complex. Current cattle vaccines against P. multocida consist of inactivated bacteria, which only offer limited and serogroup specific protection. Here,...
Preprint
Full-text available
Method: Brucella is an intracellular bacterium that affects both animals and humans. The aim of this study was to compare brucellosis diagnostic methods such as RBPT, I-ELISA, CFT and PCR detection of Brucella species. A total of (552) Sheep, (1345) Goats, and (420) Cattle sera samples were collected with no history of vaccination from brucellosis...
Preprint
Full-text available
Background Pasteurellaceae families are usually considered opportunistic pathogens which inhabit normal flora on the mucosal membranes of the upper respiratory and the lower genital tracts of mammals and birds. The majority of P. multocida, M. hemolytica, and B. trehalosi isolates are opportunistic animal pathogens and cause disease only under cert...
Article
Full-text available
Background Mannheimia haemolytica has been recognized as the principal cause of pneumonic pasteurellosis in sheep and goats. It is one of the important diseases of small ruminants in Ethiopia. While annual vaccination using a monovalent vaccine (inactivated Pasteurella multocida biotype A) is common, respiratory diseases are still reported in vario...

Questions

Questions (7)
Question
I am working on PCR pathogen detection from different host tissues. Is pathogen DNA amplification influence or interfere with host DNA? If the answer is yes, how do we know my target DNA is free from host DNA during extraction and purification?
Thanks in advance
Question
How to determine cut off cq value -qPCR assay in non-predefined mothed? is it related to total number of cycles used? specially in low copy targets.
Question
What is main difference between Ct and Cq value? When do we use Ct value and when Cq In Real Time PCR? How to determine ct/cq value in a given qPCR assay?
Question
Is antibody produced against fowlpox virus vertically transferred via eggs and further inhibited viral propagation on non-SPF cells?
Question
I faced problem on vaccine identity test those which are formalin inactivated and Alum adjuvated bacterial vaccines. Such as fowl cholera and pasteurella vaccines. Which method of bacterial DNA extraction is choice? DNeasy blood and tissue extraction kit, chloroform or heat, boiling method?
someone who have experience on these DNA extraction methods for conventional PCR works, please help me.
Thank you in advance for any help you can offer me!
Question
How to solve failurity of identity test in formalin inactivated and alum adjuvated bacterial vaccines?
Question
Q1. I am working in molecular biology laboratory. Using conventional PCR , I have got fowl cholera vaccine ( bulk ) positive results but negative results after formalin inactivation and alum adjuvation. Help me how to solve this identity test problem.

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