Publications

Figure 1: Exponentially decreasing release of ovalbumin (OVA) modulates...

Figure 3: schematic of average change in RA scores of each paw and...

Exponentially decreasing exposure of antigen generates anti-inflammatory T-cell responses

Preprint

Full-text available

Sep 2023

Rheumatoid Arthritis (RA) is a chronic debilitating disease characterized by auto-immune reaction towards self-antigen such as collagen type II. In this study, we investigated the impact of exponentially decreasing levels of antigen exposure on pro-inflammatory T-cell responses in the collagen-induced arthritis (CIA) mouse model. Using a controlled...

Fructose-1,6-biphosphate (F16BP), a glycolytic metabolite, can be...

F16BP MPs rescue DC glycolysis and function
a–d DCs treated with F16BP...

F16BP(pTRP2+PolyIC) MPs promote a pro-inflammatory response against...

F16BP MPs rescue DCs in adoptive cellular transfer (ACT) therapy model,...

F16BP MPs-based vaccines are compatible with adoptively transferred DCs...

Rescue of dendritic cells from glycolysis inhibition improves cancer immunotherapy in mice

Article

Full-text available

Sep 2023

Inhibition of glycolysis in immune cells and cancer cells diminishes their activity, and thus combining immunotherapies with glycolytic inhibitors is challenging. Herein, a strategy is presented where glycolysis is inhibited in cancer cells using PFK15 (inhibitor of PFKFB3, rate-limiting step in glycolysis), while simultaneously glycolysis and func...

Orally delivered 2D covalent organic frameworks releasing kynurenine generate anti-inflammatory T cell responses in collagen induced arthritis mouse model

Article

Jun 2023

Covalent organic framework (COF) crystalline biomaterials have great potential for drug delivery since they can load large amounts of small molecules (e.g. metabolites) and release them in a controlled manner, as compared to their amorphous counterparts. Herein, we screened different metabolites for their ability to modulate T cell responses in vit...

Biomaterial mediated simultaneous delivery of spermine and alpha ketoglutarate modulate metabolism and innate immune cell phenotype in sepsis mouse models

Article

Dec 2022

Although different metabolic pathways have been associated with distinct macrophage phenotypes, the field of utilizing metabolites to modulate macrophage phenotype is in a nascent stage. In this report, we developed microparticles based on polymerization of alpha-ketoglutarate (a Krebs cycle metabolite), with or without encapsulation of spermine (a...

Questions

What is the criteria to select a threshold for any somatic mutation in tumor to observe a functional consequence?

Question

Oct 2019

We have the RNAseq data of oral cancer(OSCC) tumor samples without matched normal. The filtered reads were of high base quality and more than 95% of base matching was considered for mutation calling. We have found several mutations in these reads (with reference to hg38).

For a particular position, we have 2% of reads having mutations. For others, we have less than 2% of the reads showing mutations. On what basis do we validate the functionality of the mutation? Is there a specific range (number of reads having the mutation) which implies that the mutation has a consequence on the function.

Any reply will be appreciated.

Thanks.