Abdulwahed Fahad Alrefaei

Abdulwahed Fahad Alrefaei
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King Saud University | KKUH · Department of Zoology

PhD

About

238
Publications
34,238
Reads
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1,805
Citations
Introduction
Molecular ecology and epidemiology professional with years of experience in Saudi Arabia and the United Kingdom. My research combines several disciplines in Ecology: animal biology, genetic classification, diversity patterns, genomic studies, pollution, biodiversity &Wild Conservation, parasites/disease in the wild and domestic bird.
Additional affiliations
December 2017 - October 2022
King Saud University
Position
  • Assosate professor
Education
April 2013 - September 2017
University of East Anglia
Field of study
  • Molecular Ecology

Publications

Publications (238)
Article
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This study provides a comprehensive assessment of the population structure, floral diversity, habitat geography, phytosociology, threats, and conservation status of Aesculus indica in the western Himalayan region of Kashmir. This study was designed to investigate distribution patterns, community structure, floristic diversity, and phytosociological...
Article
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The development of resistance to traditional antifungal therapies has necessitated the exploration of alternative treatment strategies to effectively manage fungal infections, particularly those induced by Candida albicans (C. albicans). This research investigates the possibility of integrating silver nanoparticles (AgNPs) with Terbinafine to impro...
Article
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Background C-reactive protein (CRP) is one of the most commonly monitored inflammatory markers in patients with COVID-19 to gain insight into the inflammation level in the body and to adopt effective disease management and therapeutic strategies. COVID-19 is now less prevalent, and the study of CRP as a biomarker of inflammation still needs deeper...
Article
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Chemical fertilizers have contributed to improving crop production and coping with food safety challenges, but their excessive application in agriculture has resulted in soil and environmental problems. The present study was performed to assess the potential of biostimulants as an alternative to chemical fertilizers. A pot experiment was conducted...
Article
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Pakistan, like many other regions around the world, is experiencing the impacts of climate change, particularly in its northern region. These changes have adverse impacts on ecosystems and biodiversity. Herein, we have investigated future projections of temperature and precipitation trends for three periods historical (HT = 1975–2005), near-term (N...
Article
Green tea is one of the most widely drunk beverages globally; It is made from the processed leaves of the green shrub Camellia sinensis. The background study focused on the contained catechins and their hydrolysis by tannase-producing B.subtilis KMS2-2 strains were investigated. Tannin acyl-hydrolase is an inducible enzyme extensively used in the f...
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The biodiversity of invertebrate animals is largely affected by climatic changes. This study evaluates the seasonal abundance and diversity of non-insect arthropods in the King Abdulaziz Royal Reserve (KARR), Saudi Arabia, over four collection periods (summer, autumn, winter, and spring) during 2023. Sampling was conducted across multiple sites in...
Article
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Bioprospecting robust and oleaginous strain is crucial for the commercialization of microalgae-based biodiesel. In this study, a microalgal strain SCH18 was isolated from a solar saltern located in Mauritius. This isolate was identified as Dunaliella tertiolecta based on a polyphasic approach that combined molecular, physiological, and morphologica...
Article
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Cadmium (Cd) pollution in arable soils and its accumulation in rice plants have become a global concern because of their harmful effects on crop yield and human health. The in-situ stabilization method which involves the application of organic amendments such as vermicompost (VC), is frequently utilized for the remediation of Cd-contaminated soils....
Article
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The use of biological methods to control plant diseases has garnered attention due to their eco-friendly and economically viable nature. Trichoderma spp. are the most ubiquitous fungal sap-rophytes that can often grow as opportunistic symbionts, are eco-friendly, and are not reported to exert any environmental hazard. Soil-borne pathogens can signi...
Article
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This research looks at how well different ensembled and non-ensembled machine learning algorithms perform both before and after dimensionality reduction and manual feature engineering using random feature selection. LightGBM, Extra Trees (EXT), XGBoost, Gradient Boosting Machine (GBM), Random Forest (RF), Naive Bayes (NB), K-Nearest Neighbors (KNN)...
Preprint
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Environmental monitoring and predictive modeling of the Water Quality Index (WQI) through the assessment of the water quality.
Article
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Background/Objectives: The current research work aimed to evaluate the cryptic walnut genotypes of the Hazara region in Pakistan by using DNA barcoding and phylogenetic analysis. Methods: Based on morphological traits such as nut size, nut shape, and the number of leaflets, five genotypes were chosen and samples were collected for the current study...
Article
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During a survey of microfungi associated with forest plants, four specimens related to Kirschsteiniothelia were collected from decaying wood in Guizhou, Hainan and Yunnan Provinces, China. Kirschsteiniothelia species have sexual and asexual forms. They are commonly found as saprophytes on decaying wood and have been reported as disease-causing path...
Article
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Nanotechnology is a fast-growing field with large number of applications. Therefore, the current study, was designed to prepare Zinc Oxide nanoparticles (ZnO NPs) from A. modesta leaves extract through a cost-effective method. The prepared NPs were characterized through UV-Vis Spectroscopy (UV-Vis), Dynamic light scattering (DLS), Fourier transform...
Article
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Drought stress negatively impacts agricultural crop yields. By using mineral fertilizers and chemical regulators to encourage plant development and growth, its impact can be mitigated. The current study revealed that exogenous silicon (Si) (potassium silicate; K2Si2O5 at 1000 ppm) and molybdenum (Mo) (ammonium molybdate; (NH4)6Mo7O24•4H2O at 100 pp...
Article
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Industrial waste and sewage deposit heavy metals into the soil, where they can remain for long periods. Although there are several methods to manage heavy metals in agricultural soil, microorganisms present a promising and effective solution for their detoxification. We isolated a rhizofungus, Aspergillus terreus (GenBank Acc. No. KT310979.1), from...
Preprint
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This study investigates the phytochemical profile, cytotoxic effects, and lactogenic activity of Oxystelma esculentum, a plant renowned for its traditional medicinal uses. Preliminary phytochemical screening revealed a rich array of secondary metabolites, including alkaloids, flavonoids, glycosides, and tannins, predominantly in polar solvents such...
Article
Endophytic fungal molecules have the potential to be a cost-effective chemical source for developing eco-friendly disease-controlling pharmaceuticals that target mosquito-borne illnesses. The primary aims of the study were to identify the fungus Fusarium begoniae larvicidal ability against Aedes aegypti, Culex quinquefasciatus, and Anopheles stephe...
Article
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The black soldier fly larvae Hermetia illucens L. (Diptera: Stratiomyidae) are an ideal insect to produce compost and animal feed. The goal of this study was to use Kitchen waste 50 grams, Cow dung 50 grams, and Rotten fruits 50 grams attractants to gather wild H. illucens and their egg clutches from their natural microhabitat in dumping sites. The...
Article
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As a result of increasing industrialisation in various countries and the change in their populations' eating patterns, residents may be shifting from a low-calorie diet to one with a high energy density. People tend to consume fewer fruits and vegetables and instead, they opt for more refined carbs, animal protein, processed meat, and fast food. Th...
Article
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Enhanced phytoremediation offers a rapid and eco-friendly approach for cleaning agricultural soil contaminated with copper and cadmium which pose a direct threat to food scarcity and security. The current study aimed to compare the effectiveness of the two commonly used additives, IAA and EDTA, for the remediation of copper (Cu) and cadmium (Cd) co...
Article
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Yellow passion fruit (Passiflora edulis f. flavicarpa) is valuable edible specie with economic significance in China. Passion fruit faces yield losses due to viral diseases. This study used tissue culture of nodal shoot segments to propagate virus-free plants. Surface-sterilized nodal shoot segments were used and shoots were initiated on Murashige...
Article
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Introduction Silver nanoparticles (AgNPs) have gained significance due to their practical use in the medicinal field, especially in the treatment of tumors and cancer. The current article explores a green synthetic method for the preparation of AgNPs using leaf extract of Euphorbia royleanas. Methods The synthesis was conducted at different parame...
Article
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Introduction The COVID-19 infection as an inflammatory disease has posed significant challenges to global public health due to multi-factor risks associated with it leading to disease severity and mortality. Understanding the effect of age and comorbidities on overall disease progression is crucial to identify highly susceptible individuals and to...
Article
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Inflammation coupled with oxidative stress contribute to the pathogenicity of various clinicaldisorders. Oxidative stress arises from an imbalance between production of reactive oxygenspecies (ROS) and antioxidant defense system, leading to cellular damages. The study investigatedthe antioxidant and anti-inflammatory effects of polysaccharides isol...
Article
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Citation: Khan, I.U.; Aqsa, A.; Jamil, Y.; Khan, N.; Iqbal, A.; Ali, S.; Hamayun, M.; Alrefaei, A.F.; Faraj, T.K.; Lee, B.; et al. Anti-Oxidative and Anti-Apoptotic Oligosaccharides from Pichia pastoris-Fermented Cress Polysaccharides Ameliorate Chromium-Induced Liver Toxicity. Pharmaceuticals 2024, 17, 958. Abstract: Oxidative stress impairs the s...
Article
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Among the several threats to humanity by anthropogenic activities, contamination of the environment by heavy metals is of great concern. Upon entry into the food chain, these metals cause serious hazards to plants and other organisms including humans. Use of microbes for bioremediation of the soil and stress mitigation in plants are among the prefe...
Article
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Globally, breast cancer is a primary contributor to cancer-related fatalities and illnesses among women. Consequently, there is a pressing need for safe and effective treatments for breast cancer. Bioactive compounds from endophytic fungi that live in symbiosis with medicinal plants have garnered significant interest in pharmaceutical research due...
Article
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This study explored the neuroprotective potential of fermented pomegranate (PG-F) against hydrogen peroxide (H2O2)-induced neurotoxicity in human neuroblastoma SH-SY5Y cells and elucidated the underlying molecular mechanisms. The fermentation process, involving probiotics, transforms the hydrolyzable tannins in pomegranate juice into ellagic acid (...
Article
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Animal industry seeks cost-effective solutions to enhance performance and health of domestic animals. This study investigated the effects of supplementing Bacillus spp. probiotics and xylanase on 2000 one-day-old Japanese quails, randomly assigned to four treatment groups (10 replicates). The control group received no supplementation, while the oth...
Article
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The study investigated the effects of grape seed extract (GSE) supplementation on laying hens aged 30–35 weeks. Hens were assigned to four groups: a control group with only basal diet and three experimental groups with basal diet supplemented with 250 (GSE 250), 500 (GSE 500) and 750 (GSE 750) mg/kg GSE. GSE-supplemented groups showed significantly...
Article
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The camels have attracted scientific attention due to their unique agricultural characteristics and are used for a variety of purposes, including milk and meat production. To exploit their full potential, it is essential to improve our understanding of the genetic makeup of these animals. Advances in molecular breeding have expanded our knowledge o...
Article
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Introduction Arsenate, a metalloid, acting as an analog to phosphate, has a tendency to accumulate more readily in plant species, leading to adverse effects. Methods In the current study, sunflower seedlings were exposed to 25, 50 and 100 ppm of the arsenic. Results Likewise, a notable reduction (p<0.05) was observed in the relative growth rate (...
Article
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Wolfiporia cocos, a versatile fungus acclaimed for its nutritional and therapeutic benefits in Traditional Chinese Medicine (TCM), holds immense potential for pharmaceutical and industrial applications. In this study, we aimed to optimize liquid fermentation techniques and culture medium composition to maximize mycelial biomass (MB) yield, pachymic...
Article
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The present investigation aimed to assess the impact of substituting soybean meal (SBM) with Musca domestica larvae meal (HMM) in the diet of broilers (Ross 308, n=1000) during the initial 1–28 d of their growth period. Four isocaloric and isonitrogenous broiler rations were formulated, including a control group (Mag-0) with 100 % SBM and 0 % HMM,...
Article
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Contamination of agricultural lands by the heavy metal is a serious biological and environmental issue. Such a hazard can be minimized and confronted by enhancing the growth promoting and chromium alleviating competences of fungal endophytes. Current attempt was made to find the effect of ultra violet (UV) irradiation on plant growth promoting and...
Article
Wolfiporia cocos, a versatile fungus acclaimed for its nutritional and therapeutic benefits in Traditional Chinese Medicine (TCM), holds immense potential for pharmaceutical and industrial applications. In this study, we aimed to optimize liquid fermentation techniques and culture medium composition to maximize mycelial biomass (MB) yield, pachymic...
Article
Full-text available
In this study, the freshwater microalgae Selenastrum sp. was assessed for the effective degradation of pyrene and simultaneous production of biodiesel from pyrene-tolerant biomass. The growth of algae was determined based on the cell dry weight, cell density, chlorophyll content, and biomass productivity under different pyrene concentrations. Furth...
Article
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The present study was carried out at the Plant Pathology Hafizabad Research Station, the University of Layyah, during the crop seasons 2021-2022 and 2022-2023 to evaluate the response of various wheat genotypes against leaf rust severity (%), environmental conditions favourable for disease development and grain yield. Except for minimum temperature...
Article
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Soil can function as a reservoir and a source of greenhouse gases (GHGs), contingent on its management. This study assesses the potential of a modified reduced tillage (MRT) approach involving the use of cover or green manure crops as a substitute for crop residues to mitigate GHG emissions from soil within smallholder rainfed farming systems. A tw...
Article
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The present study was carried out at the Plant Pathology Hafizabad Research Station, the University of Layyah, during the crop seasons 2021–2022 and 2022–2023 to evaluate the response of various wheat genotypes against leaf rust severity (%), environmental conditions favourable for disease development and grain yield. Except for minimum temperature...
Article
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Mango anthracnose, caused by Colletotrichum gloeosporioides, is a devastating disease that affects mango orchards in Pakistan. Although cultural practices, chemicals, and biological control are commonly used to manage the disease, the potential role of micronutrient deficiencies has been largely overlooked by farmers. In this study, four mango orch...
Article
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Diabetes mellitus is a heterogeneous metabolic disorder that poses significant health and economic challenges across the globe. Polysaccharides, found abundantly in edible plants, hold promise for managing diabetes by reducing blood glucose levels (BGL) and insulin resistance. However , most of these polysaccharides cannot be digested or absorbed d...
Article
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Classifying land use and land cover (LULC) is essential for various environmental monitoring and geospatial analysis applications. This research focuses on land classification in District Sukkur, Pakistan, employing the comparison between machine and deep learning models. Three satellite indices, namely, NDVI, MNDWI, and NDBI, were derived from Lan...
Article
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Background Guava is a fruit prone to rapid spoilage following harvest, attributed to continuous and swift physicochemical transformations, leading to substantial postharvest losses. This study explored the efficacy of xanthan gum (XG) coatings applied at various concentrations (0.25, 0.5, and 0.75%) on guava fruits (Gola cultivar) over a 15-day sto...
Article
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The trial investigated the effects of anise seed powder and protease, alone and combined, in Japanese quail diets. 540 seven-day-old quails were divided into four groups: Control (basal diet), Anise Seed Group (1000 mg/kg anise seed powder), Protease Enzyme Group (30,000 IU/kg Bacillus subtilis-derived protease enzyme), and a combination of anise p...
Article
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Background: Fusarium species, a group of economically destructive phytopathogens, are poorly studied in Mauritius where agriculture holds much significance. Furthermore, the increasing popularity of organic farming has prompted interest in alternatives to chemical fungicides. Methods: After gaining an overview of Fusarium prevalence in Mauritius fi...
Article
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In today's world, the most serious environmental and biological problem is the contamination of agricultural lands by the heavy metals. Copper is an essential element for the normal growth of plants but its concentration can build up in soil to a level that can adversely affect plant growth. Endophytic fungi associate with plants and improve their...
Article
Full-text available
The prolonged drought resulting from global warming is considered an important factor affecting West Asia’s socioeconomic growth, with a significant impact on the dynamic forecasting of water supply and forest ecosystems. In such a scenario, understanding historical long-term drought changes is crucial for accurately forecasting regional drought sh...
Article
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We hypothesized that both performance and bone mineralization would show improvements when cholecalciferol supplementation was increased above the recommended NRC (1994) [Nutrient requirements of poultry, 9th rev. ed. Washington, DC: National Academy Press] level. In this study, male Ross 308 chickens (n = 540) were supplemented with diets containi...
Article
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Background Surgical site infections are one of the major clinical problems in surgical departments that cost hundreds of millions of dollars to healthcare systems around the world. Background Nosocomial infections, including surgical site infections (SSIs) and other soft tissue infections, are a significant global health issue, particularly among...
Article
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This study aimed to identify efficient Trichoderma isolate(s) for the management of Fusarium wilt in peas. Four different pea germplasms (Sarsabz, Pea-09, Meteor and Supreme) were evaluated for resistance against Fusarium oxysporum in pot assay. Resistant germplasm exhibits a varying range of disease severity (23%) and percent disease index (21%),...