Abdullah Arpaci

Mustafa Kemal University · biochemstry

Introduction: Autoverification (AV) is a postanalytical tool that uses algorithms to validate test results according to specified criteria. The Clinical and Laboratory Standard Institute (CLSI) document for AV of clinical laboratory test result (AUTO-10A) includes recommendations for laboratories needing guidance on implementation of AV algorithms...

The aim of this study was to compare the mucosal and fecal microbiota in a high fat diet-induced metabolic endotoxemia (ME) model and to identify potential species that represent dysbiosis and might mediate the inflammatory process. Fourteen male wistar albino rats were fed a standard diet (n = 7) and a high-fat diet (HFD) (n = 7). The standard die...

The purpose of this study is to determine the volumes of primary brain regions associated with smell and taste in Alzheimer's and Parkinson's patients and healthy controls using MR imaging and examine volumetric changes in comparison to smell/taste questionnaire and test results and endocannabinoid (EC) levels. The study included 15 AD patients wit...

INTRODUCTION: People mostly live in the nonfasting state during a normal 24-h cycle. This study aims to compare the levels of 18 biochemical parameters during different hours of the day. METHODS: A total of 18 biochemical tests of patients who visited outpatient clinics only once between January 1, 2010, and December 31, 2019, were evaluated at the...

Aim The aim of this study was to make a volumetric comparison of some medial temporal lobe structures and neuropeptides between the patients of Alzheimer’s disease (AD) and healthy individuals. Method The study comprised of a group of patients diagnosed with mild AD (n:15) and a Control group (n:15) (16 females, 14 males, mean age:72.90 ± 4.50). V...

Thalassemia is a common genetic disorder. We aimed to present thalassemia mutation data that covers a period of 7 years from the Mediterranean region of Turkey by comparing with hemoglobin indices and to contribute to prenatal diagnosis and genetic counseling studies which should be decided very quickly. In this study, in which a retrospective arch...

Recurrent miscarriage is defined as the loss of an embryo / fetus less than 400 g before the completion of the 20th week of gestational age (18 weeks after fertilization) or if the gestational age is not known. Thrombophilia is thought to affect the amount or function of some coagulation factors, impairing the coagulation system and causing thrombo...

Sickle cell disease is a blood disorder that affects erythrocytes one of the most common single gene disorders in the world and the incidence is high in Turkey. Mutation in the beta globin gene leads to the formation of sickle hemoglobin tetramers. These tetramers polymerize and damage the erythrocyte membrane. Erythrocyte damage; It creates a vici...

Purpose: Despite current hypolipidemic drugs, the search for a more effective hypolipidemic agent is ongoing. In this study, it was aimed to investigate the effect of Silibinin on hyperlipidemia in rats fed high cholesterol diet (HCD). Materials and Methods: Rats were made obese. Rats were given egg yolk for 60 days and then 50 mg/kg, 100 mg/kg Sil...

Familial Mediterranean Fever (FMF), which is an autosomal recessive disease characterized by recurrent self-limiting fever, peritonitis, pleuritis, arthritis and erysipelas-like erythemas, has been common among ethnic groups such as Turkish, Armenian, Arabic and Jewish. The clinical presentation is caused by mutations in the MEFV gene encoding the...

Introduction: Blood tests are usually requested for patients visiting polyclinics in the morning following fasting. However, as hospitals provide services for 24 hours, blood is collected from patients at every hour of the day, and fasting status cannot be completely ensured. Therefore, our study aimed to compare the fasting/non-fasting biochemical...

Background: Genetic predisposition is an important risk factor in coronary artery disease (CAD).This study was conducted to determine the polymorphism frequencies of the plasminogen activator inhibitor-1(PAI-1) gene 4G/5G, Angiotensin-converting enzyme (ACE) gene I/D,and Angiotensin II type 1 receptor (AT1) gene A1166C genotypes and to examine the...

Background: Diabetes mellitus (DM) has become more and more common and has a high morbidity and mortality rate worldwide. It is a multifactorial chronic disease affected by both genetic and environmental factors. Objectives: To evaluate the association between antioxidant enzyme activities and their genetic variations and the level of malondiald...

Alzheimer’s Disease (AD) is the most common neurodegenerative disease and is hard to diagnose at the early stages. The pathogenesis of AD is associated with the loss of a sense of smell. Reduction in the volumes of the Olfactory Bulb (OB) and Entorhinal Cortex (EC) is positively correlated with the decline of the smelling function where OB projects...

Chronic kidney disease (CKD) is an important health problem across the world affecting the adult population with an enormous social and economic burden. Calcium regulation is also affected in patients with CKD, and related to several disorders including vascular calcifications, mineral bone disorders, and cardiovascular diseases (CVD). Upper zone o...

Evaluation of Biochemistry Parameters in Fasting / Nonfasting of H.M.K.U Medical Faculty Students

Bu çalışmada; kişiler, yerler, ailesel ve çevresel bilgilerin hatırlanmasında etkili olan bazı temporal lob bölümleri ile hippocampus gri cevherlerinin kortikal morfometrisinin volümetrik olarak sağlıklı kişiler ile karşılaştırılması ve nöropeptitler açısından ilgisinin incelenmesi amaçlanmıştır. Hafif dereceli Alzheimer Hastalığı (AH) tanılı hasta...

Introduction : Hypertension (HT) is characterized by high blood pressure. The reninangiotensin-aldosterone system (RAAS) plays a crucial role in blood pressure (BP) regulation by maintaining vascular tone and the water-sodium balance. We aimed to investigate whether there is any relation between AGT (M235T), ACE (I/D), and AGTR1 (A1166C) genetic po...

Apopitozis, hücrenin yaşam çemberi boyunca yapım-yıkım dengesinin sürdürülmesini sağlar. Her hücre, doğar, çoğalır (proliferasyon), farklılaşır (diferansiasyon) ve ölür (apopitozis). Doku homeostazisi yani yeniden yapım ve yıkımın bir düzen içinde oluşu, apopitozis/proliferasyon dengesinin sağlıklı bir şekilde sürdürülmesine bağlıdır. Apopitozis il...

Background: Antioxidant system enzyme activity and polymorphism were investigated in Chronic Obstructive Pulmonary Disease (COPD), which is increasingly prevalent both in the world and our country. Methods: Malondialdehyde (MDA) was measured to determine lipid peroxidation, while Catalase (CAT), Paraoxonase (PON) and Superoxide Dismutase (SOD) enz...

Background: Platelets are considered an important source of prothrombotic agents associated with inflammation in cancer related diseases. We aimed to compare the diagnostic accuracy of the platelet distribution width (PDW) and CA19-9 in resectable pancreas cancer. Method: A total of 83 stage-1 and 2 pancreatic adenocarcinoma (PAC) patients, and 85...

Background: High-mobility group box 1 (HMGB1), identified as an alarmin molecule, was shown to have a role in virus-triggered liver injury. We aimed to evaluate the association between serum levels of HMGB1 and liver fibrosis. Method: This cross-sectional case-control study included 189 chronic hepatitis B (CHB) patients and 51 healthy controls....

Aim: Oxidative stress has crucial effects over vascular pathophysiology of Sickle Cell Anemia (SCA), and contributes atherosclerosis at advanced stages of the disease. In contrast to be acknowledged the possible role of Fetuin-A in the development of atherosclerosis, its association with vascular changes in SCA appears to warrant further investigat...

Objective: Coronary artery disease (CAD) is a multifacto­rial disease that influenced by both genetic and environ­mental factors. Single nucleotide polymorphisms (SNPs) in the candidate genes produce susceptibility to such mul­tifactorial diseases. Therefore, investigations of SNPs, in the genes that may play role in etiopathogenesis of CAD, become...

Objective: Pseudocholinesterase (PChE) is an enzyme responsible for the hydrolysis of succinylcholine. In case of its deficiency, the effect of succinylcholine that is approximately 5-10 min is prolonged up to few hours. The use of succinylcholine has been declined recently. However, it is still actively used in some special conditions and in deve...

Background/aims: Contrast induced nephropathy (CIN) has multifactorial etiopatogenesis including oxidative stress and vasoconstriction. Nebivolol is an antioxidant and has vasodilatatory effect via NO release and may prevent CIN development. We have noticed that a few number of studies that have evaluated the effectiveness of nebivolol for the pre...

Background: Hepatologists have studied serologic markers of liver injury for decades. Annexins are a prominent group of such markers and annexin A2 (AnxA2) is one of the best characterized annexins. AnxA2 inhibits HBV polymerase among other functions. Its expression is up-regulated in regenerative hepatocytes. Objectives: To determine if serum A...

Background The aim of this study was to evaluate thyroid and parathyroid functions as a cause of sudden onset dizziness (SOD) in patients who were admitted to the Emergency Department (ED). Material/Methods This study was conducted prospectively in 100 patients with sudden onset dizziness (SOD) admitted to the ED. Neurologic, ear-nose-throat, deta...

ABSTRACT Aims: Dizziness is one of the most common complaints between patients referred to emergency department (ED). The spectrum of etiology is quite large and includes many unknowns, so clinicians confront huge challenges to approach in patients who for the first time refer to ED for dizziness. So far there have been inadequate investigating sys...

ABSTRACT Aims: Dizziness is one of the most common complaints between patients referred to emergency department (ED). The spectrum of etiology is quite large and includes many unknowns, so clinicians confront huge challenges to approach in patients who for the first time refer to ED for dizziness. So far there have been inadequate investigating sys...

Brucellosis is a chronic granulomatous infection and may present with various clinical manifestations. Brucellar spondylodiscitis symptoms are initially subtle and nonspecific. Annexin A2 (ANXA2) is involved in various biological functions, including osteoclast formation, bone resorption, and cell growth regulation. In this study, we aimed to deter...

Bone morphogenetic protein-7 (BMP-7) is a key protein in organogenesis and liver development. The protein has been studied in the context of liver fibrosis and regeneration. The aim of the present study was to explore any possible association between fibrosis levels (as revealed by liver biopsy) and serum BMP-7 levels. A total of 189 patients with...

Aim: To investigate the association between serum 25-hydroxyvitamin D (25(OH)D3) levels and transient tachypnea of the newborn (TTN). Methods: Calcium (Ca), phosphorus (P), alkaline phosphatase (ALP), parathormone (PTH) and serum 25(OH)D3 levels were measured in 51 infants diagnosed with TTN and 59 healthy control infants for comparison. Demogra...

Introduction and Aims: Despite modern heart failure (HF) therapy, the prognosis of patients with HF remains poor. During hospital stay acute kidney injury (AKI can complicate HF with ominous prognostic implications. Endothelial dysfunction is present in both HF and renal disease. Moreover, endothelial activation biomarker as vascular cell adhesion...

Objective: The purpose of this study is to determine the median values of the triple and double test screening parameters in Adiyaman region. Methods: Between January 2010-December 2 011, triple tests were performed to 5 909, double tests were performed to 5310 singleton pregnant women in Adiyaman Kadin-Dogum Hastanesi Biochemistry Laboratory. Seru...

Serum apoptotic cytokeratine 18 neoepitope M30 (CK-18 M30) and matrix metalloproteinase 2 (MMP-2) have been popular markers for detecting liver fibrosis in recent years. CK-18 is a major intermediate filament protein in liver cells and one of the most prominent substrates of caspases during hepatocyte apoptosis. MMP-2 plays an important role in tis...

The prevalences of hemoglobin S (HbS)and β-thalassemia (β-thal) are high in Mersin, Turkey. In this study, the results of a five-year premarital screening program in Mersin province are reported. A total of 79,000 persons including 31,498 couples were screened in this program. Hematological analyses and electrophoresis were done to identify carrier...

β-thalassemia and sickle cell anemia (SCA) are major health problems in Çukurova. Screening studies have been carried on in this region for many years. The government started premarital screening programme and prenatal diagnosis has been done in Çukurova University Medical Faculty since 1992. In spite of all these, the prevention programme has not...

Beta-thalassemia is the most common genetic abnormality causing health problems worldwide. Cukurova, in the southern part of Turkey, being on the Mediterranean, is in the thalassemic belt. Since there is no cure for the disease at present, the frequency of the mutation types of beta-thalassemia must first be identified to aid in clinical follow-up...

Purpose: This paper summarizes data obtained from the prenatal diagnosis for hemoglobinopathies of 249 fetuses. The mothers were from the southern part of Turkey where the prevalence of sickle cell anemia, β-thalassemia and α-thalassemia are 10.0, 3.7 and 3.3%, respectively, while Hb E and Hb D-Los Angeles are also not rare in this region. Methods:...

Purpose: Sickle cell anemia and thalassemia are seen most frequently in Hatay province of Turkey. These disorders are important health problems of this province. One of the important steps for preventing of these disorders is determining the carriers. Methods: In our province, a premarital screening program for hemoglobinopathy was started in 15.9....

Purpose: In prenatal diagnosis maternal DNA contamination of the CVS (chorionic villus sample) must be excluded for obtaining reliable results. The purpose of this was to study three VNTR (variable number of tandem repeats) loci by PCR for the elimination of the maternal DNA contamination in CVSs. Methods: The genomic DNA's of the couples and the C...

Serum and cervical tissue copper (Cu), zinc (Zn) and magnesium (Mg) levels were determined by atomic absorption spectrophotometry in 65 women with cervical carcinoma and compared with levels in 30 healthy women. The patients mean serum Cu level (184.8 +/- 12.3 mugr/dl) was significantly higher than the control group (p < 0.05). However, in relation...

The analytical, intra-individual, inter-individual variation and reference values were determined for red cell glucose-6-phosphate dehydrogenase (G6PD). Different procedures for the conditions for storage of red blood cells and the preparation of haemolysates were investigated. A total of 2170 samples of blood were taken from apparently healthy per...

The distributions of twelve beta-thalassemic mutations in samples (n = 139 chromosomal samples) from four regions of Turkey were determined. The frequencies of these mutations did not reveal a notable region specific heterogeneity. In particular, the four mutations, IVS.1/nt.110(G/A), IVS.1/nt.6(T/C), IVS.1/nt.1(G/A) and nonsense codon.39(C/T), wit...

Blood samples were taken from farmworkers (n=40) who had been long-term exposed with pesticides (16.52±6.92 years) in ‹çel and surrounding agricultural areas and measured erythrocyte superoxide dismutase (SOD) and catalase (CAT) activities were measured using spectrophotometric assignation. These antioxidant enzymes were also evaulated in people wh...

Blood samples were taken from agricultural workers who had been exposed to pesticides for long periods in ‹çel and the surrounding agricultural areas (n=150) and levels of AST, ALT, ALP, LDH, total protein, albumin and globulin in serum were determined. The same parameters were measured in 64 persons who are not exposed to pesticides in any way. St...

ZET Genetik hastalıklar içinde önemli bir yer tutan hemoglo-binopatiler, Çukurova Bölgesinde bazı yörelerde % 8-10'lara varan oranlarda görülmektedir. Otozomal resesif geçişli hu hastalığın kuşaklar boyu devam etmesi ailelere ve topluma maddi ve manevi yük olmaktadır. 1992 yılı Mayıs ayından başlayarak heterozigot Thalasse-mi veya Orak hücre anemis...