Abdul Nasir Khalid

Abdul Nasir Khalid
University of the Punjab | PU · Department of Botany

Ph.D. Botany (Mycology)

About

592
Publications
254,436
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5,890
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Additional affiliations
January 1989 - present
University of the Punjab
Position
  • Professor

Publications

Publications (592)
Article
Full-text available
Zhuliangomyces pakistanicus is described and illustrated as a new species from Pakistan based on morphology and phylogeny. It is characterized by a depressed sticky creamy white pileus, an ixo-trichodermal pileipellis with lageniform terminal elements, a glutinous stipe, and obovoid smooth basidiospores. Phylogenetically, this is a distinct species...
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With only three published reports, the genus Chroogomphus (Boletales, Gomphidiaceae) is poorly studied in Pakistan. During recent sampling events in Khyber Pakhtunkhawa province, Pakistan, several collections of Chroogomphus were made, representing undescribed taxa. Based on morphological and molecular data, two new species are described: Chroogomp...
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Pseudosperma flavorimosum is a new species described from Khyber Pakhtunkhwa province, Pakistan. It is delimited based on morphological characters combined with a molecular phylogeny inferred from nuclear ribosomal DNA internal transcribed spacer (ITS) sequence analyses. The ITS-based phylogeny supports the independence of the new species, which is...
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Inocybe crenata sp. nov. (subsec. Geophyllinae, sect. Tardae) has been described from conifer dominated forests of Pakistan. Inocybe crenata differs from other related species by having a pale-yellow pileus with crenate margins and a bumpy or cracked center, with a fibrillose stipe. Phylogenetic analysis based on nuclear ribosomal large subunit (ls...
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A new species Pseudosperma parvisporum is described and illustrated here, based on morpho-anatomical and molecular phylogenetic characterization. The taxon is characterized by campanulate to becoming plano-convex to convex pileus at maturity, having 3.4-4.5 cm diam., with brown umbo, brownish golden to yellow fibrils spread on; up to 8 cm stipe, ho...
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During macrofungal surveys in 2019–2024, several specimens belonging to the family Psathyrellaceae were collected from the bed of the Indus River, Punjab, Pakistan. Phylogenetic analyses, based on ITS, LSU, and tef-1α sequences and morpho-anatomical study, confirmed the novelty and placement of three taxa in the genus Candolleomyces. They are descr...
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Agaricus is a genus of saprophytic fungi in the Basidiomycota. As part of ongoing studies on the genus Agaricus in Pakistan, we here describe and discuss two new species, A. pakistanicus sp. nov. and A. sparsisquamosus sp. nov. Morphological characters and phylogenetic analysis of ITS sequences were used to clarify their taxonomic affinities. Phylo...
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Gymnopilus dunensis is described as a new species from Punjab province Pakistan, based on internal transcribed spacer (ITS) sequence data and morphological characters. Presence of grey scales on the pileus, slightly radicating and fibrillose stipe that is white at the apex and orange-brown at the base, and its unique phylogenetic position confirm t...
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This article is the 17th in the Fungal Diversity Notes series which allows the researchers to publish fungal collections with updated reports of fungus-host and fungus-geography. Herein we report 97 taxa with four new genera distributed in three phyla (Ascomycota, Glomeromycota and Mucoromycota), 11 classes, 38 orders and 62 families collected fr...
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Morphological and molecular phylogenetic analyses were conducted to identify Hohenbuehelia species collected during 2020–2023 in Lahore, Margalla Hills, and Abbottabad in the Khyber Pakhtunkhwa region of Pakistan. The phylogenetic analysis was based on the internal transcribed spacer region (ITS) of nuclear ribosomal DNA. Among collected taxa, we i...
Article
The new species Verrucaria aptrootii sp. nov. is described from Darel Valley, Gilgit Baltistan, Pakistan. A comparative morpho-anatomical, chemical study and ITS-based molecular analyses confirmed the position of this new species within the genus Verrucaria. Verrucaria aptrootii sp. nov. is closely related to V. squamulosa and characterized by a no...
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A new taxon in the genus Immersaria was discovered during a lichen survey in Darel Valley and Killa Saifullah, Pakistan and it was determined to be I. pruinosa sp. nov. The main characteristics of I. pruinosa sp. nov. are flat to slightly concave, blackish brown, up to 1 mm apothecia, taller hymenium 80-140 µm, apically branched paraphyses, smaller...
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Circinaria terrenoides sp. nov. was collected in Balochistan and Gilgit Baltistan. Comparative morpho-anatomical research, chemical analysis , and ITS-based DNA analyses all support its placement within the genus Circinaria. Circinaria terrenoides sp. nov. is distinguished from the closely related species C. affinis in having a crustose, areolate t...
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A new species, Infundibulicybe swatica is described and illustrated from District Swat, Khyber Pakhtunkhwa, Pakistan. The new taxon is characterized by pale yellow to pale yellow brown to brown pileus with a dark brown center; a bicolorous stipe, white at the apex and pale yellow brown hues at the base; hyaline, lacrymoid to sub-lacrymoid, small, i...
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In this study, we describe Pseudomegasporoporia margallensis as a novel species and report Microporellus subadustus as a new record from Pakistan based on morphology and phylogeny. Both genera are firstly reported in the country. Pseudomegasporoporia margallensis sp. nov. is characterized by light brownish gray poroid hymenphore, grayish brown subi...
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With the simultaneous growth in interest from the mycological community to discover fungal species and classify them, there is also an important need to assemble all taxonomic information onto common platforms. Fungal classification is facing a rapidly evolving landscape and organizing genera into an appropriate taxonomic hierarchy is central to be...
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In a detailed investigation of lichen diversity in different regions of Pakistan, utilizing molecular and morphological techniques, we identified and reported three species – Dimelaena altissima, Lecanora chlarotera, and Usnea lapponica – as new records for Pakistan. Furthermore, nrITS sequences for Evernia prunastri, Lecanora horiza, L. hybocarpa,...
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A new species of Russula has been collected from Punjab and Khyber pakhtunkhwa provinces, Pakistan. Russula punjabensis sp. nov. is characterized by grayish purple to grayish red pileus with brittle central region, white lamellae with brown striations, becoming brown at maturity, a rhizoidal base, small (7.48 × 6.64 µm) subglobose to broadly ellips...
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Basidiomata belonging to the genus Candolleomyces were collected from plains of Punjab, Pakistan, and characterized based on morpho-anatomical and phylogenetic analyses. In phylograms nrITS & nrLSU regions were used to ascertain exact positions of our new taxa in this genus. Three novel taxa, Candolleomyces granulosis, C. pabbiensis and C. umbonatu...
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Phytochemical composition and antimicrobial, antibiofilm, and antioxidant effects of a newly described Himalayan lichen Placidium deosaiense Usman and Khalid growing in Pakistan were investigated. HPLC–DAD methods were used for identification of secondary metabolites in acetone and methanol extracts. The total phenolics content was measured using a...
Article
This study describes a new species of genus Rhodocybe (Fr.) Maire, R. pakistanica sp. nov., from Pakistan. The novel species can be recognized morphologically by convex to hemispherical or applanate to planoconvex and centrally depressed pileus which is dull reddish brown at the center and dull orange pale at margins having bright patches, light ye...
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The new species Gloeoheppia immersusia sp. nov. is described from Darel Valley, Gilgit Baltistan, Pakistan. A comparative morpho-anatomical and chemical study and ITS-based molecular analyse indicated a position of this new species within the genus Gloeoheppia Gyeln. Gloeoheppia immersusia sp. nov. is closely related to G. turgida (Ach.) Gyeln and...
Article
Taxonomic investigations on the family Entolomataceae were conducted in Pakistan from 2018 to 2022. Morphological characteristics and molecular phylogenetic analyses using ITS and LSU sequences revealed two new species, Entoloma pakistanicum and Rhodophana margallensis, and a new record, E. albotomentosum. Rhodophana margallensis is the first repor...
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Taxonomic investigations on the family Entolomataceae were conducted in Pakistan from 2018 to 2022. Morphological characteristics and molecular phylogenetic analyses using ITS and LSU sequences revealed two new species, Entoloma pakistanicum and Rhodophana margallensis, and a new record, E. albotomentosum. Rhodophana margallensis is the first repor...
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This study reports the first discovery of Rinodina moziana, a previously unrecorded lichen species in Pakistan, identified during a field survey in the Malakand division, Khyber Pakhtunkhwa. Detailed morphological, chemical and internal transcribed spacer (ITS)-based phylogenetic analyses confirmed the species’ identity, marking it as Rinodina mozi...
Preprint
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Lichenochemical composition and antimicrobial, antibiofilm and antioxidant effects of a newly described arctic lichen Placidium doesaiense growing in Pakistan were investigated. HPLC–DAD methods were used for identification of secondary metabolites in acetone and methanol extracts. The total polyphenol and the total flavonoid content was determined...
Article
The purpose of this study is to explore the genus Candolleomyces from Pakistan. Fungal surveys were conducted in different sites of the Punjab plains during the 2016-2022 period. Several specimens belonging to the family Psathyrellaceae were collected. Traditional morphology and multigene phylogenetic analyses confirmed the novelty and placement of...
Preprint
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During the field survey conducted in Malakand district, Khyber Pakhtunkhwa, Pakistan, Rinodina species was discovered. The taxonomic and chemical characters along with an ITS-based phylogenetic analysis and notes on ecology and distribution considered it as Rinodina moziana (lichenized Ascomycota). A comprehensive study of the available literature...
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The genus Agaricus includes more than 500 species mostly containing the edible and cultivated species worldwide. As part of the ongoing studies on the biodiversity of genus Agaricus in Pakistan, our objective was to focus on A. sect. Minores which is the largest section of the genus. In the first phylogenetic analyses based on the ITS region of the...
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A new record, Aspicilia fumosa, is being reported for the first time from Pakistan in an examination of Aspicilia species in Darel Valley. A description of the species is provided together with details on its distribution, habitat and phylogeny.
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A new species, Candolleomyces amygdaliformis, is described from Pakistan, based on morpho-anatomically, and phylogenetic analysis of combined ITS and LSU sequences. Morpho-anatomically, the new taxon is characterized by a light brownish gray pileus with a reddish brown center and a decurved margin, a light gray, shiny stipe, and amygdaliform to pha...
Article
Onnia pakistanica, a novel species, is described from Khyber Pakhtunkhwa, Pakistan. This species is distinguished from its closest kin, O. tibetica, by its unique rusty orange to clay-buff and fawn pileus, larger (5.0–10.1 × 2.5–9.0 μm) basidiospores, broader and fusoidal cystidioles (11.1–16 × 6.5–8.6 μm) and setae with more or less rounded at the...
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A new species, Lecanora darelensis sp. nov. is described here from Darel Valley, Gilgit Baltistan, Pakistan. A comparative morpho-anatomical, chemical study and ITS-based molecular analyses confirmed the position of new species within the genus Lecanora. The new taxon is phylogenetically closely related to L. argopholis. The former can be distingui...
Article
For the first-time, chemical composition and in vitro antitumor activity was investigated of a newly described lichen Anamylopsora pakistanica Usman & Khalid from the second highest plateau of the world (Deosai Plains, Pakistan). HPLC-UV method was used for identification of secondary metabolites and the acetone extract had higher values of TPC (41...
Article
Four taxa viz., Lecidea polypycnidophora, Peltigera ponojensis, Phaeophyscia cernohorskyi and Rinodina milvina belonging to three different families are being reported here as new to Pakistan. Detailed descriptions of each species, habitat, general distribution and diagnostic features are presented along with biochemical analysis. Keywords: folio...
Article
During a survey of the lichen flora of the Darel Valley in Gilgit Baltistan and the Sharan Valley in Khyber Pakhtunkhwa many lichen specimens were collected. These specimens were analyzed using morpho-anatomical, molecular, and chemical test methods. Two species new to Pakistan, Lecidella flavosorediata and Pertusaria qilianensis, and one new to Gi...
Article
A new species, Entoloma khushabense, is described and illustrated here based on morphological and molecular evidence from Pakistan. The key features of the new species that make it distinct from closely related taxa include the presence of conspicuous cheilocystidia and caulocystidia, and hyphae having constrictions in the walls. Morphological data...
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The genus Trichoderma holds economic significance due to its widespread distribution and diverse applications, including biological control, enzyme production, and various biotechnological uses. The accurate identification of Trichoderma species is crucial given their close association with human activities. Despite previous efforts in classificati...
Article
A crustose species Circinaria semicontorta is described here as new to science. It is characterized by a whitish areolate to subverrucose thallus with a thinning, cracked margin, not forming true lobes, areoles with raised whitish pseudocyphellae containing black ostioles of conidiomata, and long pycnoconidia, 12–25 μm. The phylogenetic analysis ba...
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Leucopaxillus pakistanicus sp. nov. is described and illustrated from Upper Dir, Khyber Pakhtunkhwa, Pakistan, based on a comprehensive description of morphology and phylogeny. Leucopaxillus pakistanicus is characterized by brick red or red-brown, slightly thin fleshed, creamy short and decurrent thick lamellae, long, slender and fistulose stipe, g...
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Candelariella darelensis sp. nov. is described from Darel Valley, Gilgit Baltistan, Pakistan, an area with a cold semi-arid climate. Its placement within the genus Candelariella Müll.Arg. was validated by a comparative morpho-anatomical investigation and an analysis of ITS sequences. Candelariella darelensis sp. nov. is closely related to C. rosula...
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Chlorangium ahmadii sp. nov. and Circinaria darelensis sp. nov. are described as new species from Pakistan. A comparative morpho-anatomical, chemical study and ITS-based molecular analyses confirmed the positions of these species within the genera Chlorangium and Circinaria. Chlorangium ahmadii sp. nov. differs from its closely related species, C....
Article
The genus Mallocybe is poorly studied in Asia, with little known species diversity of the genus in Himalayan forests of Pakistan, a hotspot for biodiversity. Based on morphological characters and molecular data, two new species of Mallocybe from Himalayas of Asia are described. One species, Mallocybe himalayana has been collected from Himalayas of...
Article
The genus Mallocybe is poorly studied in Asia, with little known species diversity of the genus in Himalayan forests of Pakistan, a hotspot for biodiversity. Based on morphological characters and molecular data, two new species of Mallocybe from Himalayas of Asia are described. One species, Mallocybe himalayana has been collected from Himalayas of...
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During our studies on the genus Lepiota in Pakistan, we collected two putatively new species from Punjab Province, with distinct morphology, ITS, and 28S of nrDNA profile. L. aurantiopilea is featured by orange to yellow-orange pileus with a reddish-brown umbo, tiny granules on the surface that are concolorous to pileus, absence of annulus, subglob...
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Members of the lichen-forming fungal genus Oxneriaria are known to occur in cold polar and high altitudinal environments. Two new species, Oxneriaria crittendenii and O. deo-saiensis, are now described from the high altitude Deosai Plains, Pakistan, based on phe-notypic, multigene phylogenetic and chemical evidence. Phenotypically, O. crittendenii...
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Two new species under the names of Lepiota brunneopileata and L. pakistanensis are described herein based on four collections during mushroom surveys in 2021-2022 from scrubland in district Gujrat, Pakistan. Morpho-anatomy and phylogenetic placement, based on Internal Transcribed Spacer (nrITS) and Larger Subunit (nrLSU), clustered them into Lepiot...
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During exploratory surveys of the fungal diversity in Margalla Hills National Park, Islamabad, we collected a new species of the genus Hymenopellis R.H.Petersen. This is the second report of any species of this genus from Pakistan. Hymenopellis areolata F.Razzaq & Khalid sp. nov. is characterized by an areolate pileus, small basidiospores, and tran...
Article
Based on morphological and molecular data, Bonomyces pakistanensis is described as a new species found in coniferous forests of Pakistan. Several collections of Bonomyces were made during mycological field trips conducted in different coniferous forests in Khyber Pakhtunkhwa, Pakistan. Detailed macro‐ and micro‐morphological descriptions and phylog...
Article
Two new species of the lichen genus Lecaimmeria are described and illustrated from Pakistan, namely, Lecaimmeria asiatica and L. crispula. The former taxon is characterized by pale brown to dark brown thallus, up to 2-mm large areoles, flat to strongly convex apothecial disc, taller hymenium 120–150 µm, apically branched, not anastomosing paraphyse...
Article
The new species Diploschistes iqbalii sp. nov. is described from Darel Valley, Gilgit Baltistan, Pakistan. A comparative morpho-anatomical, chemical study and ITS-based molecular analyses confirmed the position of this new species within the genus Diploschistes Norman. Diploschistes iqbalii sp. nov. is closely related to D. muscorum (Scop.) R. San...
Preprint
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A new species in genus Inocybe is described and illustrated from Himalayan Moist Temperate forests of Pakistan. It is characterized by fibrillose, conical to convex, umbonate, brown to dark brown pileus, non-pruinose, fibrillose stipe with whitish tomentum at base, basidiospores smooth and comparatively larger (9 × 5.2 µm) and thicker caulocystidia...
Preprint
Pisolithus albus, collected from two different provinces is reported as a new record from Pakistan based on morphological and molecular analyses. This taxon is identified by off-white basidiomata with round, echinulate basidiospores. Phylogenetically this taxon belongs to P. albus complex. Morphological description, illustrations and phylogenetic p...
Article
Several basidiomata of the genus Moniliophthora were found on plant debris, from the bed of the Indus River, Kot addu, and an irrigated plantation in Chichawatni, Sahiwal, Pakistan. the morpho-anatomical examination along with phylogenetic analyses support the independent position of Moniliophthora purpurensis and M. capitata within the genus Monil...
Article
We analyzed smut fungi (Ustilaginales) parasitizing nine poaceous hosts from three districts (Shangla, Mansehra, and Dir Upper) of Khyber Pakhtunkhwa Province, Pakistan. Eight taxa were identified in two genera using both morphological and molecular techniques by amplifying ITS and LSU regions. We describe Ustilago pseudosyntherismae sp. nov. on Di...
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Circinaria russus sp. nov. is described based on morpho-anatomical and phylogenetic analysis. The novel species is characterized by its reddish brown thallus, absence of marginal lobes, taller hymenium 175-190 μm, and larger ascospores 22-32 × 15-20 than the similar taxon Circinaria tortuosa. A phylogenetic analysis, based on ITS nrDNA sequencing a...
Article
As part of comprehensive study of lichen diversity of northern areas of Pakistan using molecular and morphological approaches, we found three species of family Lecanoraceae belonging to genus Omphalodina and Rhizoplaca. The analyses revealed one new record in the lichen biota of Pakistan namely Rhizoplaca parilis while the other two Omphalodina chr...
Article
A new powdery mildew species, Erysiphe neelamensis , was found on Celtis eriocarpa in Azad Jammu and Kashmir, Pakistan. The identification was based upon morpho‐anatomical features and results of a molecular sequence analysis. This species is morphologically distinguished from phylogenetically allied Erysiphe species by having chasmothecia with few...
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How the multiple facets of soil fungal diversity vary worldwide remains virtually unknown, hindering the management of this essential species-rich group. By sequencing high-resolution DNA markers in over 4000 topsoil samples from natural and human-altered ecosystems across all continents, we illustrate the distributions and drivers of different lev...