Abdelhak EL Amrani

Université de Rennes 1 | UR1 · UMR CNRS 6553 - Ecosystème-Biodiversité-Evolution (ECOBIO)

Environmental contamination by xenobiotics represents a major threat for natural ecosystems and public health. In response, xenobiotic detoxification is a fundamental trait of organisms for developmental plasticity and stress tolerance, but the underlying molecular mechanisms remain poorly understood in plants. To decipher this process, we explored...

In this paper, the concentration of As, Cd, Cr, Cu, Pb, and Zn was investigated in soil and Ambrosia artemisiifolia L. sampling from polluted cite near the enterprises for the production and processing of batteries in the city of Dnipro in Ukraine. The obtained results of the study were provided to assess the plant species through bio-monitoring an...

Plants have developed strategies to adapt quickly to environmental changes. However, the regulation of these adaptive responses and coordination of signals network remains poorly understood for many environmental constraints. Indeed, signalling molecules play a central role in environmental stimuli and may coordinate plants development under enviro...

The importance of microorganisms in plant development, nutrition, and stress resistance is unquestioned and has led to a more holistic approach of plant–microbe interactions, under the holobiont concept. The structure of the plant microbiota is often described as host driven, especially in the rhizosphere, where microbial communities are shaped by...

Spartina spp. are widely distributed salt marsh plants that have a recent history of hybridization and polyploidization. These events have resulted in a heightened tolerance to hydrocarbon contaminants, but the effects of this phenomenon on the rhizosphere microbial communities is unknown. Here, we grew two parental Spartina species, their hybrid a...

In the present work, we used a double cell screening approach based on phenanthrene (phe) epifluorescence histochemical localization and oxygen radical detection to generate new data about how some specialized cells are involved in tolerance to organic xenobiotics. Thereby, we bring new insights about phe [a common Polycyclic Aromatic Hydrocarbon (...

Key Message Differential expression of mi-RNAs targeting developmental processes and progressive downregulation of repeat-associated siRNAs following genome merger and genome duplication in the context of allopolyploid speciation in Spartina. Abstract The role of small RNAs on gene expression regulation and genome stability is arousing increased i...

Xenobiotic detoxification is a common trait of all living organisms, necessary for developmental plasticity and stress tolerance. The gene set involved in this biological process is dubbed the xenome (i.e. involved in drug metabolism in mammals, degradation of allelochemicals and environmental pollutants by bacteria and plant communities). Recently...

Genome doubling or polyploidy is a widespread phenomenon in plants where it has important evolutionary consequences affecting the species distribution and ecology. PAHs are ubiquitous organic pollutants, which represent a major environmental concern. Recent data showed that tolerance to organic xenobiotics involve specific signaling pathways, and d...

Despite the severe impacts of the Deepwater Horizon oil spill, the foundation plant species Spartina alterniflora proved resilient to heavy oiling, providing an opportunity to identify mechanisms of response to the anthropogenic stress of crude oil exposure. We assessed plants from oil affected and unaffected populations using a custom DNA microarr...

A highly diverse plant community in a constructed wetland was used to investigate an ecological treatment system for human wastewater in an arid climate. The eight-year operation of the system has allowed the identification of a highly adapted and effective plant consortium that is convenient for plant-assisted metaremediation of wastewater. This c...

Background Higher plants have to cope with increasing concentrations of pollutants of both natural and anthropogenic origin. Given their capacity to concentrate and metabolize various compounds including pollutants, plants can be used to treat environmental problems - a process called phytoremediation. However, the molecular mechanisms underlying t...

It was shown that halophytes experience higher cross-tolerance to stresses than glycophytes, which was often associated with their more powerful antioxidant systems. Moreover, salinity was reported to enhance halophyte tolerance to several stresses. The aim of the present work was to investigate whether a moderate salinity enhances phenanthrene str...

Whole genome duplication (polyploidization) is an important process in the evolutionary history of plants. The “genomic shock” induced by the union of divergent genomes may induce structural and functional dynamics, which represents an important source of variability and new adaptive abilities. As polyploidization has various impacts on regulation...

Halophytes are plants able to tolerate high salt concentrations but no clear definition was retained for them. In literature, there are more studies that showed salt-enhanced tolerance to other abiotic stresses compared to investigations that found enhanced salt tolerance by other abiotic stresses in halophytes. The phenomenon by which a plant resi...

This review summarises recent knowledge of polycyclic aromatic hydrocarbons (PAHs) biotransformation by microorganisms and plants. Whereas most research has focused on PAH degradation either by plants or microorganisms separately, this review specifically addresses the interactions of plants with their rhizosphere microbial communities. Indeed, pla...

There is currently a major challenge to improve photosynthetic yield for increased sustainable crop production and meeting mankind's nutritional requirements. Among other factors, suboptimal carbon partitioning and suboptimal photoprotection may contribute to limitations of photosynthetic efficiency (1). Herbicide and oxidative stress tolerance can...

Herbicide and oxidative stress tolerance can involve mechanisms that are related to photosynthesis efficiency and carbon dynamics. In this context, an Arabidopsis thaliana T-DNA insertional mutant was identified and characterized for enhanced tolerance to the singlet-oxygen-generating herbicide atrazine in comparison to wild-type.This enhanced atra...

Abstract In a previous study, we showed that the halophyte plant model Thellungiella salsuginea was more tolerant to phenanthrene (Polycyclic Aromatic Hydrocarbon: PAH) than its relative glycophyte Arabidopsis thaliana. In the present work, we investigated the potential of another halophyte with higher biomass production, Cakile maritma, to reduce...

In a previous study, we showed that the halophyte plant model Thellungiella salsuginea was more tolerant to phenanthrene (Polycyclic Aromatic Hydrocarbon: PAH) than its relative glycophyte Arabidopsis thaliana. In the present work, we investigated the potential of another halophyte with higher biomass production, Cakile maritma, to reduce phenanthr...

Polycyclic aromatic hydrocarbons (PAHs) form a large family of persistent hydrophobic pollutants. They were recently ranked 9th on the “CERCLA priority list of hazardous substances”, which makes them of high priority for remediation efforts. PAHs remediation often consists of using chemical or physical treatments. recently, phytoremediation was eme...

A highly diverse plant community in a constructed wetland was used to investigate an ecologicaltreatment system for human wastewater in an arid climate. The eight-year operation of the system hasallowed the identification of a highly adapted and effective plant consortium that is convenient for plant-assisted metaremediation of wastewater. This con...

Environmental constraints challenge cell homeostasis and thus require a tight regulation of metabolic activity. We have previously reported that the γ-aminobutyric acid (GABA) metabolism is crucial for Arabidopsis salt tolerance as revealed by the NaCl hypersensitivity of the GABA transaminase (GABA-T, At3g22200) gaba-t/pop2-1 mutant. In this study...

A method is provided for phytoremediating a site polluted by at least one type of pollutant, comprising at least one step comprising growing, on this polluted soil, at least one phytoremediating plant capable of fixing at least a portion of this pollutant, and comprising a subsequent step comprising of harvesting or destroying this plant that has f...

Localization of T-DNA insertion in eat1 mutant line by Southern blot analysis. Five µg of genomic DNA from eat1 mutant were separately digested with HindIII and NdeI and resulting DNA fragments were separated by agarose gel electrophoresis and then blotted onto a nylon membrane. Hybridization was carried out with specific DIG-labelled probes corres...

Enhanced biomass phenotype of BH755830 Arabidopsis mutant line. Root and shoot fresh weights are given. Seeds of the BH755830 Arabidopsis mutant line were germinated on 1x MS-agar medium in the absence of atrazine, and plantlet development was carried out for 15 days. Values are the mean (± S.E.M.) of measurements on at least sixteen 15-day-old pla...

An Arabidopsis thaliana T-DNA insertional mutant was identified and characterized for enhanced tolerance to the singlet-oxygen-generating herbicide atrazine in comparison to wild-type. This enhanced atrazine tolerance mutant was shown to be affected in the promoter structure and in the regulation of expression of the APL4 isoform of ADP-glucose pyr...

GABA (γ-aminobutyric acid), a non-protein amino acid, is a signaling factor in many organisms. In plants, GABA is known to accumulate under a variety of stresses. However, the consequence of GABA accumulation, especially in vegetative tissues, remains poorly understood. Moreover, gene expression changes as a consequence of GABA accumulation in plan...

Application number: EP 8803646.2; Pub. n° US 2010/0323890 - Dec 23, 2010

GABA (gamma-aminobutyric acid) is a non protein amino acid that has been reported to accumulate in a number of plant species when subjected to high salinity and many other environmental constraints. However, no experimental data are to date available on the molecular function of GABA and the involvement of its metabolism in salt stress tolerance in...

Growth of pop2-1 mutant under low K+ conditions. Phenotype of 10-day-old plants grown on agar media containing 500, 50 or 5 μM potassium. Potassium was deleted from nutrient solution by replacing KNO3 and KH2PO4 with NH4NO3 and NH4H2PO4 respectively, potassium concentration was set by addition of KCl. Scale bar = 1 cm. Experiment was performed twic...

UPLC- and GC-MS-based metabolite profiling dataset. Absolute values of metabolites levels are given in this excel sheet.

pPOP2::GUS expression pattern in primary root apex. Histochemical analysis of POP2 promoter activity in primary root apex under control conditions.

Molecular and physiological characterization of POP2-overexpressing lines. (A) POP2 expression in 11-day-old plantlets WT and the three 2 × 35S::POP2 lines. Stars indicate a significant difference with WT according to non-parametric Mann-Whitney U-test (P < 0.05). (B) GABA content in 14 day-old plantlets of WT and two POP2 overexpressing lines trea...

Phenotype of siliques of POP2-overexpressing plants. Phenotype of siliques of 60-day-old plants alimented since their 14-day-old stage with standard nutrient solution supplemented, or not (Control), with 50 mM NaCl. Scale bar = 0.5 cm.

List of verified primer pairs used for qRT-PCR analysis. Sequence accessions used for primers design are indicated.

Response of pop2-1 mutant to various kinds of toxic cations. Phenotype of 10-day-old plants treated for 6 days with, or without (Control), 1 mM spermidine or 100 μg/ml kanamycin. Scale bar = 1 cm. Experiment was performed three times with same results.

Primary root growth response of POP2-overexpressing lines to NaCl and GABA. Primary root growth of POP2-overexpressing lines on agar plates supplemented, or not (Control), with 150 mM NaCl (NaCl), or 150 mM NaCl + 10 mM GABA (NaCl+GABA). Experimental procedures are the same as reported in figure 4. Different letters indicate a significant differenc...

BACKGROUND: GABA (gamma-aminobutyric acid) is a non protein amino acid that has been reported to accumulate in a number of plant species when subjected to high salinity and many other environmental constraints. However, no experimental data are to date available on the molecular function of GABA and the involvement of its metabolism in salt stress...

proprietaires : CNRS et Université de Rennes I

Physiological effects of atrazine and sucrose treatments. Arabidopsis plantlets were grown on Murashige and Skoog agar medium and transferred at the 1.02 development stage [20] to Murashige and Skoog agar medium supplemented with mannitol (80 mM), mannitol (80 mM) plus atrazine (10 μM), sucrose (80 mM) and sucrose (80 mM) plus atrazine (10 μM). Pic...

Effects of atrazine and sucrose treatments on chlorophyll content. Arabidopsis plantlets transferred to Murashige and Skoog agar medium supplemented with mannitol (80 mM)(A), sucrose (80 mM)(B), mannitol (80 mM) plus atrazine (10 μM)(C), and sucrose (80 mM) plus atrazine (10 μM)(D) were harvested after 0, 1, 4 and 8 days of treatment for pigment de...

Overview of the 810 highly-responsive genes. Additional file 6 lists genes whose log2(ratio) was greater than 1.585 or lesser than -1.585 (corresponding to 3-fold change) in at least one of the MA/M, SA/M or S/M comparisons in the CATMA array experiment. The full data were deposited in ArrayExpress [78] (E-MEXP-411).

Statistical significance of gene repression and induction for the different functional categories. The statistical analysis using a χ2 test was realized on each functional category of genes in order to compare the significance of induction and repression between the different comparisons (MA/M, S/M, SA/M).

Repression by atrazine of genes in DNA and protein dynamics. Additional file 9 lists several genes involved in DNA and protein dynamics and repressed by atrazine treatment in the present study.

Effects of atrazine and sucrose treatments on carotenoid content. Arabidopsis plantlets transferred to Murashige and Skoog agar medium supplemented with mannitol (80 mM)(A), sucrose (80 mM)(B), mannitol (80 mM) plus atrazine (10 μM)(C), and sucrose (80 mM) plus atrazine (10 μM)(D) were harvested after 0, 1, 4 and 8 days of treatment for pigment det...

Effects of atrazine and sucrose treatments on photosystem II efficiency (Fv/Fm). Arabidopsis plantlets were transferred to Murashige and Skoog agar medium supplemented with mannitol (80 mM)(A), sucrose (80 mM)(B), mannitol (80 mM) plus atrazine (10 μM)(C), and sucrose (80 mM) plus atrazine (10 μM)(D); chlorophyll fluorescence and maximum PSII effic...

Sucrose treatment in Arabidopsis thaliana: comparison of its transcriptomic effects with previous studies. Additional file 10 lists several genes corresponding to typical markers of carbohydrate responses according to previous studies and found responsive to sucrose treatment in the present study.

Validation of microarray results using real-time PCR. The log2(relative quantity to the control sample mannitol) of the qRT-PCR (open) obtained for 8 selected genes was compared with the log2(intensity ratio) of the CATMA array analysis (striped) using RNA from plantlets transferred during 24 h to mannitol-atrazine (blue), sucrose (red) or sucrose-...

Genes selected for qRT-PCR analysis and primer sequences. Additional file 12 lists genes selected for qRT-PCR analysis and primer sequences.

Schematic representation of experimental procedure for end-point CATMA array analysis. Six comparisons of mannitol-, sucrose-, mannitol-atrazine- and sucrose-atrazine-treated plantlets were performed at the end of a 24 h treatment. Double arrows indicate dye-swap hybridisation.

Conserved cis-acting elements and their percentage of occurrence in the promoter region of genes presenting high induction by sucrose-atrazine combination. Additional file 11 lists cis-acting regulatory elements and their occurrence in promoters of genes presenting high induction by the sucrose-atrazine combination and corresponding transcription f...

Helitrons are a class of prolific transposable elements in the Arabidopsis thaliana genome. Although 37 families were identified after the recent discovery of Helitrons, no systematic classification is available because of the high variability of helitronic sequences. Since transposition proteins are assumed to interact with Helitron termini, a Hel...

Supplementary Material 3. Comparison of sequences detected by RepeatMasker and STAN. Sequences detected by both methods are in purple, those detected only by RepeatMasker are in blue, and those by STAN in red. The right insert details the number of common sequ... Sébastien Tempel, Jacques Nicolas, Abdelhak El Amrani, Ivan Couée Model-based identifi...

Supplementary Material 2. Comparing detection of Helitrons by RepeatMasker and STAN. X corresponds to " absence of results ". The second column corresponds to the number of occurrences of each family described by Kapitonov and Jurka (2001). The third column corr... Sébastien Tempel, Jacques Nicolas, Abdelhak El Amrani, Ivan Couée Model-based identi...

Soluble sugars can induce tolerance to otherwise lethal concentrations of the herbicide atrazine in Arabidopsis thaliana seedlings. This sugar-induced tolerance involves modifications of gene expression which are likely to be related to sugar and xenobiotic signal transduction. Since it has been suggested that ethylene- and sugar-signalling pathway...

Growth in the presence of sucrose was shown to confer to Arabidopsis thaliana (thale cress or mustard weed) seedlings, under conditions of in vitro culture, a high level of tolerance to the herbicide atrazine and to other photosynthesis inhibitors. This tolerance was associated with root-to-shoot transfer and accumulation of atrazine in shoots, whi...