Abd Al-Bar Ahmed Al-Farha

• PhD, MSc, BVM&S
• Professor (Associate) at Northern Technical University

Background Mycoplasma mastitis is increasingly posing significant impact on dairy industry. Although the effects of major conventional mastitis pathogens on milk components has been widely addressed in the literature, limited data on the effects of different Mycoplasma and Acholeplasma spp. on milk quality and quantity is available. The aim of this...

Abstract Objectives: This study aimed to provide a rapid, accurate and cost-effective diagnostic real time polymerase chain reaction-high resolution melting curve assay (PCR-HRM) to identify and distinguish between four different mycoplasmas and Acholeplasma laidlawii isolated at cow-level from a single commercial dairy farm in South Australia. One...

Abstract Objectives Currently, there is no consensus protocols regarding the combination of glycerol (GLY), gelatin or foetal bovine serum (FBS) with dimethyl sulphoxide (DMSO) as cryoprotectants for Mycoplasma bovis in bovine milk samples. This study aimed to compare different cryopreservation compounds and storage temperatures for M. bovis. Resu...

How to Cite This Article Al-Farha AAB, Hemmatzadeh F, Tearle R, Jozani R, Hoare A, PetrovskI K: Comparison of culture and PCR for detection of field isolates of bovine milk mollicutes. Kafkas Univ Vet Fak Derg, 26 (3): 337-342, 2020. Abstract Mycoplasma mastitis raises significant concerns in the dairy industry worldwide. The study objective was to...

The objective of this study was to detect Mycoplasma bovis specific antibodies using a recently reported MilA ELISA with the aim to detect M. bovis antibodies in milk. An indirect ELISA, based on a recombinant fragment of the Mycoplasma immunogenic lipase A (MilA) protein, was conducted on 291 milk samples for the detection of M. bovis antibodies....

Background: Moringa oleifera has garnered significant interest. This research evaluated the impact of different quantities of Moringa oleifera seed powder on certain productive and physiological characteristics in Awassi ewes. The research investigated the effects on lamb development and birth outcomes within a sheep herd managed by Rashidiyah Rese...

T HE objective of this study was to evaluate of nano-zinc oxide and zinc sulfate on thyroid hormones, histomorphometry and microbial in the small intestine. A total of 108 Rose 308 chicks (1d old) were randomly divided into six groups with three replicates per group, each having 18 birds. Treatments include: group one (control with basal diet),grou...

T HE present study was to estimate the influence of nano-zinc oxide and zinc sulfate on some hematological parameters, liver enzymes, glutathione and malondialdehyde. A total of 108 Rose 308 chicks (1d old) were randomly divided into six groups with three replicates per group (18 birds per group). Treatments included: group one (control with basal...

A study was conducted to elucidate the prescence of T.gondii antibodies in the serum of slaughtered cattle, sheep & goats in Mosul abattoir. The study was carried out during the period from October 2002 to March 2003. For serological tests, 300 serum samples were collected from (100) of each slaughtered cattle, sheep & goats. Serum samples collecte...

The objective of this review is to compile and assess the findings of several studies on the use of Rocket (Eruca sativa) as feed additives or alternatives to fish nutrition. Antioxidant glucosinolates, flavonoids, and vitamins A and C are present in this plant, which has been shown to improve growth metrics and blood characteristics, according to...

The current study was carried out from October 15, 2021, to June 15, 2022, in the area of animal production at the Agricultural Technical College, Northern Technical University, Mosul, Iraq. 24 Awassi ewes between the ages of 2-3, were employed in the study and split into two groups. In the first group, 12 ewes had winter lambs in December–January...

The current study was carried out from October 15, 2021, to June 15, 2022, in the area of animal production at the Agricultural Technical College, Northern Technical University, Mosul, Iraq. 24 Awassi ewes between the ages of 2-3, were employed in the study and split into two groups. In the first group, 12 ewes had winter lambs in December–January...

To examine the effects of adding seeds powder and rocket Eruca sativa leaves to the diets of common carp Cyprinus carpio L. fish with percentages of 1, 2, and 3% of the total diet (the first, second, and third treatments), as well as the addition of rocket leaf powder to the fourth, fifth, and sixth diets, respectively, While the control diet, was...

Blunt trauma account around 95% of injuries. Approximately 15% of the overall trauma injuries affect the abdomen and considered a leading cause of death in young adults. Although Laparotomy is considered as a standard procedure for managing traumatized cases, laparoscopic techniques increasingly used as an alternative procedure. The aim of the curr...

A better understanding of milk alteration during mastitis at genus-species level of causative pathogen is essential to guide prospective control strategies. The current study aimed to identify the correlation between individual genus or species of pathogens causing bovine mastitis and alteration of milk volume and composition. The study was conduct...

Aims: The aim of this study was assessing a phage displayed MilA protein of Mycoplasma bovis in an indirect ELISA for the detection of M. bovis antibodies in milk samples. Methods and results: The desired sequence of milA gene was synthesized and cloned into pCANTAB-F12 phagemid vector. The expression of the MilA on the phage surface was confirm...

Ice cream is a popular frozen milk products worldwide. In the same time, maple syrup is one of the few products in the Middle East, Asia and Iraq, that has been used as a natural local substance instead of sucrose. This food product as health and nutritional benefits compared to sucrose. That's why this research aims to assess the extent to which m...

Bovine ringworm is a serious public and veterinary health problem worldwide. Many antifungals and antibiotics have been evaluated in the past in an attempt to identify a suitable agent for the topical treatment of bovine ringworm. The objective of this study was to highlight the epidemiology of ringworm in cattle in North of Mosul; and to evaluate...

This study aims to identify the chemical composition of purslane and examine its antimicrobial activities against two types of pathogenic bacteria: Pseudomonas savastanoi, and Staphylococcus aureus. Ether petroleum extract and ethanol extract were extracted using a Soxhlet extractor. Saponification was undertaken to separate the fatty acids from th...

ABSTRACT Mycoplasma mastitis has been recognised as an emerging disease with a significant impact on the dairy industry. This study aimed to determine the genotypic distribution of some Mycoplasma and Acoleplasma spp isolated from two commercial dairy farms in South Australia based on 16S rRNA sequencing, and compare their evolutionary relationship...

Mycoplasma mastitis has been recognised as an emerging disease with a significant impact on the dairy industry. This study aimed to determine the genotypic distribution of some Mycoplasma and Acoleplasma spp isolated from two commercial dairy farms in South Australia based on 16S rRNA sequencing, and compare their evolutionary relationship to some...

Öz Mycoplasma mastitisleri dünya çapında süt endüstrisinde önemli sorunlara yol açmaktadır. Çalışmanın amacı, Mycoplasma ve Acholeplasma türlerinin saha izolatlarının tanımlanması için doğru ve hızlı bir tarama yöntemi geliştirmek ve inek sütünde Mycoplasma ve Acholeplasma türlerinin saptanması için PCR tabanlı yönteme karşı geleneksel mikrobiyal k...

Abstract. Thirty of weaned male rabbits of 4-5 weeks of age and average (534 ± 1.5g) in weight were divided into three treatments of 10 rabbits per treatment and used in 8 weeks feeding trial for production efficiency. The rabbits were fed one ration that contains crude protein (15.8%) and metabolizable energy (2865.5 Kcal/ Kg feed). The turmeric p...

Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translate...

ABSTRACT A total of 125 drought male horses in different ages were used, among these 100 animals were naturally suffering from colicky signs and 25 clinically normal horses served as control. Results indicated to the presence of different etiological factors exhibited the colic syndrome, such as dietetic causes concerned with quality and quantity o...

The objective of this study was to detect Mycoplasma bovis specific antibodies using a recently reported MilA ELISA with aim to detect M. bovis antibodies in milk. An indirect ELISA, based on a recombinant fragment of the Mycoplasma immunogenic lipase A (MilA) protein, was conducted on 291 milk samples for the detection of M. bovis antibodies. Samp...

The ultimate aims of this thesis were to improve the diagnostics of mollicute-associated mastitis and confirm their pathogenic role in bovine mastitis. Three types of experiments were carried out on improving diagnostics of mollicute associated mastitis (PCR, real time PCR-high resolution melting curve analysis (HRM), and Enzyme linked immunosor...

Objectives: Mycoplasma mastitis is increasingly generating considerable interest in bovine dairy industry. It is common knowledge that mycoplasmas can cause clinical or subclinical mastitis in cattle either as an individual or co-invader pathogen. Mycoplasma mastitis is usually be a missing component from routinely mastitis detection due to the spe...

Methicillin-resistant coagulase-negative staphylococci (MRCoNS) have recently emerged as a significant cause of bovine mastitis worldwide. Here we describe the isolation of MRCoNS from cases of bovine mastitis from a single dairy farm in Australia. Fourteen CoNS isolates were identified as MRCoNS on the basis of having an oxacillin MIC of ≥0.5 μg/m...

The study was conducted on 48 native male calves aged between 6-18 months; 33 calves were naturally infected with Theileria annulata and 15 calves were clinically normal which served as a control. Results indicated that the clinically infected calves showed clinical signs including fever, anorexia, loss of appetite, respiratory manifestation, enlar...

A total of 125 drought male horses in different ages were used, among these 100 animals were naturally suffering from colicky signs and 25 clinically normal horses served as control. Results indicated to the presence of different etiological factors exhibited the colic syndrome, such as dietetic causes concerned with quality and quantity of feeding...

The objective of this study was to investigate the coagulation disorders in drought horses suffering from colic. A total of (125) local drought male horse of different ages were investigated, among these animals (100) were suffering colicky sings and (25) clinically normal drought male horses served as control. Results indicated that colicky horses...

Abstract: The objective of this study was to investigate the coagulation disorders in drought horses suffering from colic. A total of (125) local drought male horse of different ages were investigated, among these animals (100) were suffering colicky sings and (25) clinically normal drought male horses served as control. Results indicated that coli...