Abbye McEwen

Abbye McEwen
University of Washington Seattle | UW · Department of Pathology

About

21
Publications
1,011
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
372
Citations
Citations since 2016
7 Research Items
208 Citations
201620172018201920202021202201020304050
201620172018201920202021202201020304050
201620172018201920202021202201020304050
201620172018201920202021202201020304050
Additional affiliations
July 2016 - June 2020
University of Washington Seattle
Position
  • Medical Doctor
October 2010 - December 2014
Northwestern University
Position
  • PhD Student
June 2003 - January 2006
University of Texas at Dallas
Position
  • Master's Student

Publications

Publications (21)
Article
Radiation-associated sarcomas are an uncommon complication of therapeutic radiation. However, their prevalence has increased with the more widespread use of this treatment modality. The clinical, pathologic and genetic characteristics of radiation-associated sarcomas are not fully understood. In this study we describe the features of 94 radiation-a...
Preprint
Full-text available
A central problem in genomics is understanding the effect of individual DNA variants. Multiplexed Assays of Variant Effect (MAVEs) can help address this challenge by measuring all possible single nucleotide variant effects in a gene or regulatory sequence simultaneously. Here we describe MaveDB v2, which has become the database of record for MAVEs....
Article
Full-text available
Across 20 vaccine breakthrough cases detected at our institution, all 20 (100%) infections were due to variants of concern (VOC) and had a median Ct of 20.2 (IQR=17.1-23.3). When compared to 5174 contemporaneous samples sequenced in our laboratory, VOC were significantly enriched among breakthrough infections (p < .05).
Preprint
Full-text available
Across 20 vaccine breakthrough cases detected at our institution, all 20 (100%) infections were due to variants of concern (VOC) and had a median Ct of 20.2 (IQR=17.1-23.3). When compared to 5174 contemporaneous samples sequenced in our laboratory, VOC were significantly enriched among breakthrough infections (p < .05).
Article
Full-text available
Genetic data are rapidly becoming part of tumor classification and are integral to prognosis and predicting response to therapy. Current molecular tumor profiling relies heavily on tissue resection or biopsy. Tissue profiling has several disadvantages in tumors of the central nervous system, including the challenge associated with invasive biopsy,...
Article
Introduction Glioblastoma multiforme (GBM) is the most aggressive and most common primary brain tumor. Treatment of GBM and other high-grade gliomas consists of surgical resection followed by radiation and temozolomide (TMZ) chemotherapy. TMZ alkylates purine DNA bases, causing DNA damage and eventually cell death. Alkylation at O6-gauanine can be...
Article
Distinguishing neoplastic from reactive cutaneous lymphoid infiltrates remains a diagnostic challenge. While flow cytometry is broadly used in hematopathology, it is only now emerging as a method for determining the immunophenotype and clonality of cutaneous lymphocyte populations. Cutaneous marginal zone lymphomas are particularly challenging; the...
Article
Full-text available
E-cadherin is highly phosphorylated within its β-catenin binding region, and this phosphorylation increases its affinity for β-catenin in vitro. However, the identification of key serines responsible for most cadherin phosphorylation and the adhesive consequences of modification at such serines have remained unknown. In this study, we show that as...
Data
Expression analysis of GFP-ZFN2 linker variants.
Data
Inter-finger linker strategy to expand target half-sites from 9 to 10 bp.
Data
Amino acid sequence of GFP-ZFN2 DNA-binding domain.
Data
Nuclease activities of GFP-ZFN2 inter-finger linker variants.
Article
Full-text available
Recent studies have shown that zinc finger nucleases (ZFNs) are powerful reagents for making site-specific genomic modifications. The generic structure of these enzymes includes a ZF DNA-binding domain and nuclease domain (Fn) are separated by an amino acid "linker" and cut genomic DNA at sites that have a generic structure (site1)-(spacer)-(site2)...
Article
The cadherin/catenin complex organizes to form a structural Velcro that joins the cytoskeletal networks of adjacent cells. Functional loss of this complex arrests the development of normal tissue organization, and years of research have gone into teasing out how the physical structure of adhesions conveys information to the cell interior. Evidence...
Article
Full-text available
Agarose-gel electrophoresis has been used for more than thirty years to characterize the linking-number (Lk) distribution of closed-circular DNA molecules. Although the physical basis of this technique remains poorly understood, the gel-electrophoretic behavior of covalently closed DNAs has been used to determine the local unwinding of DNA by prote...
Data
Comparison of calculated enhanced repression as a function of operator spacing with the in-vivo data of Müller et al. [4] (0.27 MB DOC)
Data
Supporting information for “Analysis of In-Vivo LacR-Mediated Gene Repression Based on the Mechanics of DNA Looping” by Yongli Zhang, Abbye E. McEwen, Donald M. Crothers, and Stephen D. Levene. (0.31 MB DOC)
Data
Comparison of calculated enhanced repression as a function of operator spacing with the in-vivo data of Becker et al. [5] for wild-type (WT) and HU-deletion (ΔHU) E. coli strains. (0.27 MB DOC)
Article
The lack of a rigorous analytical theory for DNA looping has caused many DNA-loop-mediated phenomena to be interpreted using theories describing the related process of DNA cyclization. However, distinctions in the mechanics of DNA looping versus cyclization can have profound quantitative effects on the thermodynamics of loop closure. We have extend...
Article
Full-text available
Interactions of E. coli lac repressor (LacR) with a pair of operator sites on the same DNA molecule can lead to the formation of looped nucleoprotein complexes both in vitro and in vivo. As a major paradigm for loop-mediated gene regulation, parameters such as operator affinity and spacing, repressor concentration, and DNA bending induced by specif...

Network

Cited By