A. A. Soldatov

A. A. Soldatov
Russian Academy of Sciences - Institute of Marine Biological Reseach · Deparment - Animal Physiology and Biochemistry

Prof., Ph. D., D. Sci.

About

242
Publications
22,500
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
981
Citations
Additional affiliations
June 2009 - August 2015
Russian Academy of Sciences, Moscow, Russia · A.O. Kovalevsky Institute of Marine Biological Research Message
Position
  • Head of Department

Publications

Publications (242)
Article
Full-text available
The relationship between the contents of abnormal erythroid forms (dacryocytes, nuclear invagination, and erythrocyte ghosts) and the number of immature erythrocytes (pronormoblasts, as well as basophilic and polychromatophilic normoblasts) was studied in the circulating blood of round gobies. It was found that the proliferative activity of the ery...
Book
Full-text available
The monograph provides a detailed summary of the species diversity of the Black sea malacofauna. Special attention is paid to the state of some molecular systems and the dynamics of metabolic processes in the tissues of mass species of mollusks. A significant part of this monograph is devoted to antioxidant enzyme complex in tissues. For the first...
Article
Full-text available
The in vivo and in vitro effect of hypoxia (0.57–8.17 mg О2 l–1) on functional characteristics of nucleated erythrocytes of the benthic scorpion fish Scorpaena porcus L. was studied. The cellular systems in this species were established to be characterized by a balanced suppression of membrane and metabolic functions in conditions of external oxyge...
Article
Full-text available
Data are comprehended on the mechanisms of hypoxic states development in the primary-aquatic organisms. The ways of modification of actual classification of hypoxic states were proposed on the base of primary and secondary hypoxia conceptions. New types of exogenous, hemic, locomotor and diffusion hypoxia, character mainly for the aquatic organisms...
Article
The effect of hypoxia on the expression level of pyruvate kinase (PKM), octopine dehydrogenase (OcDH) and malate dehydrogenase (MDH) genes in the gill tissue of the Mediterranean mussel Mytilus galloprovincialis (L., 1819) was studied experimentally. The control group of mollusks was kept at 9–10°C and the oxygen level in the water was 8.5 mgO2 l–1...
Article
The content of the membrane-bound methemoglobin fraction (MtHb) in the nuclear erythrocytes of the sea ruff (Scorpaena porcus, Linnaeus, 1758) was studied in vitro. The spectral characteristics of whole hemolysate, hemolysate after stroma deposition (purified hemolysate), and resuspended stroma were studied. It was found that the proportion of MtHb...
Article
Anadara kagoshimensis (Tokunaga, 1906) is a bivalve alien species of the Black Sea and of the Azov Sea. The amino acid composition of hemolymph and protein hydrolysates of the mollusc soft tissues was studied. The content of 16 proteinogenic amino acids in the samples was determined by ion-exchange chromatography followed by ninhydrin detection. Hi...
Article
The content of membrane-bound methemoglobin (MtHb) in nucleated erythrocytes was studied in the black scorpionfish Scorpaena porcus (Linnaeus, 1758) in vitro. Spectral characteristics were determined for a whole hemolysate, a hemolysate obtained by stroma precipitation (a clarified hemolysate), and a resuspended stroma. The MtHb proportion in the e...
Article
Abstract—Anadara kagoshimensis (Tokunaga, 1906) is an invasive bivalve mollusk colonizing the BlackSea and Azov Sea. The content of 16 proteinogenic amino acids was determined in their hemolymph andsoft tissue protein hydrolysates via ion-exchange chromatography followed by ninhydrin detection. Highhistidine and proline levels were detected both in...
Article
The erythroid lineage was studied in the flounder-gloss (Platichthys flesus Linnaeus, 1758) during the annual cycle. The erythrocyte count in the blood was determined along with the contents of immature erythroid forms (basophilic and polychromatophilic normoblasts) in the head kidney (pronephros) and the blood. Cell proliferative activity was infe...
Article
The effect of moderate hypoxia on the processes of energy metabolism in the tissues (gills, hepatopancreas) of the Mediterranean mussel (Mytilus galloprovincialis, Lamarck, 1819) was studied experimentally. The control group of mollusks contained 6.8–6.9 mgO2 l–1, the experimental group at 1.9–2.0 mgO2 l–1. In both cases, the water temperature was...
Article
Anadara kagoshimensis (Tokunaga, 1906) is an invasive species that has successfully colonized the waters of the Black Sea and the sea of Azov, despite the significantly lower salinity level of these waters in comparison with the native region. The morphological and morphometric characteristics of bivalve mollusk A. kagoshimensis erythrocytes were a...
Article
The changes in the number of erythrocytes in the blood and the production of erythrocytes by the hematopoietic tissue of the golden grey mullet (Chelon auratus Risso, 1810) during the annual cycle were studied. Catching and delivery of fish to the aquarium was carried out monthly. The content of immature erythroid forms was determined: erythroblast...
Article
Bivalve mollusks as typical osmoconformers are unable to maintain a constant level of internal osmolarity in conditions of salinity stress. Adaptation to fluctuations of environmental salinity is achieved through cellular osmoregulatory responses, which are accompanied with a substantial shift in functional state of cells. In the present work we in...
Article
Full-text available
The activity and expression level of cytoplasmic malate dehydrogenase (MDH 1.1.1.37) gene and adenylate system state in tissues of the Mediterranean mussel Mytilus galloprovincialis Lamarck, 1819 were investigated. The highest values of adenylate energy charge (AEC) (0.66 ± 0.01) and adenylate content (ATP, ADP, and AMP) were observed in the mollus...
Article
In the present study, the structure of the fructose-1,6-bisphosphataldolase (FBA) gene in Mytilus galloprovincialis (Lamarck, 1819) was analyzed and its tissue specificity of expression level and activity was determined. A 1092 base pairs (bps) complete coding sequence of the FBA gene was assembled from M. galloprovincialis transcriptome. Only one...
Article
The in vitro effect of cold shock on erythrocytes of the benthic scorpionfish Scorpaena porcus L. was studied. The blood samples were exposed to 22°С (control group) and 4°С (experimental group) for 3 hours. Cold shock had no significant impact on erythrocyte scorpionfish vital function. The cells maintained the integrity of cytoplasmic membranes,...
Preprint
Full-text available
The changes in the number of erythrocytes in the blood and the production of erythrocytes by the hematopoietic tissue of the golden grey mullet ( Chelon auratus Risso, 1810) during the annual cycle were studied. Catching and delivery of fish to the aquarium was carried out monthly. The content of immature erythroid forms was determined: pronormobla...
Article
The effect of 30-day starvation on the state of the antioxidant complex and lipid peroxidation in the tissues of the Black Sea bivalve mollusks Anadara kagoshimensis (Tokunaga, 1906) was studied. Mollusks were collec-ted on a mussel and oyster farm near the village Katsiveli (the Southern coast of Crimea, the Black Sea) on April 2020. The mollusks...
Article
The natural mobility of the organisms affects the functional state and development of many organ systems and tissues. It influences, first of all, oxygen supply systems: respiratory, circulatory, blood. Of particular interest are molecular complexes whose functional characteristics are determined at the genetic level. These include respiratory pigm...
Article
This study describes the analysis of antioxidant enzymatic activities (catalase and superoxide dismutase) in gills and functional state of hemocytes (osmotic stability, mitochondrial membrane potential) of ark clams (Anadara kagoshimensis) from the Black Sea basin exposed to salinity stress. For this, the effects of 48 h periods of exposure to low...
Article
Full-text available
In the present work, we studied the effect of short-term acute hypoxia on the cellular composition of the blood and the head kidney of the black scorpionfish. Dissolved oxygen concentration was decreased from 8.5–8.7 mg O2 l⁻¹ (normoxia) to 3–5 mg O2 l⁻¹ (relative normoxia), 1–3 mg O2 l⁻¹ (moderate hypoxia), and 0–1 mg O2 l⁻¹ (acute hypoxia) within...
Article
Full-text available
The activity of oxidoreductases, malate dehydrogenase and lactate dehydrogenase (MDH, 1.1.1.37; LDH, 1.1.1.27), as well as parameters of adenylate system—[ATP], [ADP], [AMP], total adenylate pool (AP), and adenylate energy charge (AEC) in medulla oblongata (MB) and forebrain, midbrain, and diencephalon (FDMB)—were studied in the scorpionfish under...
Article
Full-text available
Костистые рыбы известны как экспериментальные модели для изучения физиологических и патофизиологических процессов, в частности связанных с работой сердца. Методы, позволяющие производить анализ частотных характеристик сердечного ритма в течение длительного периода времени, нуждаются в учёте особенностей поведенческих реакций рыб, способных повлиять...
Article
In the present work we investigated the influence of three days anoxia on hematological parameters, morphological and functional characteristics of eryhroid cells of alien bivalve Anadara kagoshimensis . Oxygen concentration in seawater was decreased by bubbling with nitrogen gas for 5 h. Temperature was maintained at 20±1oC and photoperiod was 12h...
Article
Impact of low salinity on morphology and function of hemocytes in ark clam species Anadara kagoshimensis was investigated using light microscopy and flow cytometry. In control group the water salinity was adjusted to 19.6‰, and experimental group was maintained at 14.8‰ and 8.8‰. Two cell types, amebocytes and erythrocytes, were identified in contr...
Article
The activities of cytosolic oxidoreductases (malate and lactate dehydrogenases) and the level of ATP production in the hypoxic resistive tissues of Scorpaena porcus Linnaeus, 1758 were studied. It was found that “oxyphilic” tissues (structures of the brain, gills) are pre-adapted to hypoxia, since under normal conditions they had high MDH activity...
Article
Full-text available
Shellfish farms are usually located in coastal areas, where molluscs can be exposed to hypoxia. Cultivating at low oxygen levels causes general disruptions of growth rate, outbreaks of diseases, and mollusc mortality. Impact of short-term hypoxia on hemocyte functions of ark clam (Anadara kagoshimensis) was investigated by flow cytometry. A control...
Article
The effect of hypoxia on nucleated red blood cells of the black scorpionfish (Scorpaena porcus) was studied in vitro. Deep hypoxia (the oxygen concentration was less than 1 mg O2 L–1; the norm was 7–8 mg O2 L–1) led to the transition of a part of the hemoglobin molecules to the ferric state (methemoglobin). The maximum increase in the concentration...
Article
Full-text available
Рассматриваются теоретические аспекты адаптации морских организмов к зонам острой гипоксии. Показано, что гипоксические акватории оказывают комплексное действие на организм гидробионтов, которое проявляется в сочетании следующих факторов: острой гипоксии; гипотермии; гипербарии и гиподинамии. Ведущим среди них является острый дефицит кислорода, зна...
Article
The influence of hypothermia on erythrocyte profile of thermophile teleost species round goby, Neogobius melanostomus (Pallas, 1814), has been studied. Fish were acclimated to temperature 1-2оС, 15-16оС and 19-20оС (control group) and held at given conditions for 5 days. The number of red blood cell precursors (pronormoblasts, basophilic and polych...
Article
Full-text available
В условиях эксперимента изучали влияние краткосрочной гипоксии на активность оксиредуктаз (малат- и лактатдегидрогеназы: МДГ, ЛДГ) в структурах головного мозга и жабр (передняя дуга) морского ерша, определяющих процессы срочной адаптации организма к дефициту кислорода. Контрольная группа рыб содержалась при 4.5–6.7 мг O2·л-1 (нормоксия), опытные гр...
Article
Full-text available
Fish red blood cells (RBCs) exhibit an oxygen-dependent regulatory volume decrease (RVD) in hypoosmotic environment. In higher vertebrates, membrane-associated hemoglobin is involved in the regulation of osmotic ion movements across the cellular membrane. However, whether the hemoglobin conformational state plays a role in the regulation of osmotic...
Article
Circulating hemocytes of ark clam, Anadara kagoshimensis, were investigated using light microscopy and flow cytometry. Hemolymph contained 3 morphotype of cells, amebocytes, erythrocytes and intermediate type cells, which formed two distinct subpopulations on flow cytometric dot plots. Large cells (intermediate morphotype and erythrocytes) amounted...
Article
Full-text available
The influence of anoxia on the content and composition of carotinoids in the tissues of the bivalve invader Anadara kagoshimensis (Tokunaga, 1906)- Borodina A.V., Soldatov A.A. - Under the experimental conditions, the unfluence of experimental anoxia on the content and qualitative composition of carotenoids in the tissues of the bivalve invader Ana...
Article
Full-text available
The work examined the influence of experimental anoxia on the content and qualitative composition of carotenoids in the tissues of the bivalve invader Anadara kagoshimensis (Tokunaga, 1906) under experimental conditions. The oxygen content in the water was reduced by nitrogen sparging for five hours. The exposure lasted three days. Tissue samples (...
Article
Full-text available
l-Lactate/malate dehydrogenases (LDH/MDH) and type 2 l-lactate/malate dehydrogenases (LDH2/MDH2) belong to NADH/NADPH-dependent oxidoreductases (anaerobic dehydrogenases). They form a large protein superfamily with multiple enzyme homologs found in all branches of life: from bacteria and archaea to eukaryotes, and play an essential role in metaboli...
Article
Full-text available
The effect of short-term hypoxia on the black scorpionfish (Scorpaena porcus L.) red blood cells has been investigated. Fish were exposed to 8.5–8.7 mg O2·l-1 (100 % saturation, control), 2.6 mg O2·l-1 (30 % saturation) and 1.3 mg O2·l-1 (15 % saturation) for 90 minutes. At 30 % saturation all parameters were kept at the control values. At oxygen-s...
Preprint
Full-text available
L-lactate/malate dehydrogenases (LDH/MDH) and type 2 L-lactate/malate dehydrogenases (LDH2/MDH2) belong to NADH/NADPH-dependent oxidoreductases (anaerobic dehydrogenases). They form a large protein superfamily with multiple enzyme homologs found in all branches of life: from bacteria and archaea to eukaryotes, and play an essential role in metaboli...
Article
Full-text available
В условиях краткосрочного эксперимента исследовали влияние острой гипоксии на морфо-функциональное состояние эритроцитов морского ерша (Scorpaena porcus L. 1758). Рыб содержали при 8,5–8,7 мгO2•л-1 (100% насыщение, контроль), 2,6 мгO2•л-1 (30% насыщение) и 1,3 мгO2•л-1 (15%насыщение) в течение 90 минут. При 30% насыщении воды кислородом не наблюдал...
Book
Full-text available
The most dangerous invasive species of Russia (TOP-100) / Eds. Yu.Yu. Dgebuadze, V.G. Petrosyan, L.A. Khlyap. M.: KMK Scientific Press, 2018, 688 p. The book is devoted to biological invasions of alien species. It is the first presentation of the list and summarized information on the 100 worst invasive species (TOP-100) in Russia which can pose th...
Article
Full-text available
To identify cells and analyze proliferative activity of hematopoietic tissue, black scorpionfish head kidney and spleen cells were characterized by light microscopy and flow cytometry. Hematopoiesis of black scorpionfish head kidney was formed by the following series: erythropoietic, granulopoietic, lymphopoietic, and thrombopoietic. Flow cytometri...
Article
Full-text available
В условиях острого эксперимента исследовано влияние ранжированной гипоксии на напряжение кислорода в артериальной, венозной крови (PaO2, PvO2) и скелетных мышцах (PmO2) кефали-сингиля (Liza aurata, Risso, 1810). Одновременно по содержанию лактата и величинам рН в крови и мышечной ткани оценен уровень анаэробных процессов в скелетных мышцах рыб. Кон...
Article
Full-text available
The effect of the combined action of hypoxia (0.05 mg O2/L) and hydrogen sulfide contamination (4–8 mg S2–/L) on the morphometric characteristics of the bivalve mollusk Anadara kagoshimensis (Tokunaga, 1906) was studied under experimental conditions in vivo; the exposure lasted for 24 hours. A significant increase in the size of granular inclusions...
Article
Full-text available
NEW METHODOLOGICAL APPROACH TO EXPRESS ASSESSMENT OF ECOLOGICAL STATE (HEALTH) OF COASTAL SEA WATERS S.V. Kholodkevich, T.V.Kuznetsova, A.S.Kurakin, A.A. Soldatov, O.L. Gostukhina, I.V.Golovina, T.I. Andreenko, M.P.Kirin SUMMARY In the present paper we propose a new methodological approach for express assessment of ecological state of the coast...
Article
Full-text available
New methodological approach to express assessment of ecological state in the coastal areas of the Black Sea is proposed on the base of functional state evaluation for mussel Mytilus galloprovincialis. Non-invasive cardiac activity of mussels under a short-term functional loading (salinity change) was monitored in four differently polluted sites at...
Article
Full-text available
The variability of the nucleotide sequences of the fragment of the COI gene of the female genome was studied in four phenotypic groups of the Black Sea mollusk Mytilus galloprovincialis. It was shown that the parameters of genomic diversity (the number of haplotypes, haplotypic and nucleotide diversity, and the number of nucleotide substitutions, i...
Conference Paper
Full-text available
Антиоксидантная ферментная система гепатопанкреаса мидий проявила высокую чувствительность к естественным состояниям, а жабр — к морской среде. Количественные же изменения активностей ферментов были близкими. Рекомендуется для целей экодиагностики у гидробионтов выбирать жаберную ткань. / The antioxidant enzyme system of the mussel’s hepatopancrea...
Article
Red blood cells of vertebrates can restore their cellular volume after hyposmotic swelling. The process strictly depends on oxygen availability in the environment. However, the role of hemoglobin in regulation of cell volume recovery is not clear yet. Little is known about the osmotic reactions and regulatory volume decrease of amphibian red blood...
Article
Full-text available
Analysis of own and literature data shows that oxygen tension and mass transfer in skeletal muscles of higher and lower vertebrates (mammals, teleosts) are quite comparable. Oxygen consumption in fish muscles is 2—6 times lower and occurs at higher diffusion gradients of PO2 (blood muscles: 45—57 hPa). Weighted average values of PO2 in fish muscles...
Article
Analysis of own and literature data shows that oxygen tension and mass transfer in skeletal muscles of higher and lower vertebrates (mammals, teleosts) are quite comparable. Oxygen consumption in fish muscles is 2–6 times lower and occurs at higher diffusion gradients of PO2 (blood ↔ muscles: 45–57 hPa). Wei ghted mean values of PO2 in fish muscles...
Article
Full-text available
Обобщена информация о напряжении и диффузии кислорода в скелетных мышцах костистых рыб. Сопоставлены методы регистрации и расчета данных характеристик. Отмечено, что диффузия кислорода в скелетных мышцах костистых рыб протекает более сложно, чем у высших позвоночных. Допускается, что этот процесс ограничивается мембранными структурами клеток мышечн...
Article
Full-text available
В условиях эксперимента in vivo исследовали влияние сочетанного действия гипоксии (0.05 мгО2/л) и сероводородной нагрузки(4−8 мгS2-/л) на морфометрические характеристики эритроцитов двустворчатого моллюска Anadara kagoshimensis (Tokunaga, 1906) в течение 24 ч. Отмечено значительное увеличение размеров гранулярных включений в клетках. Число включени...
Article
Full-text available
The influence of hypoxia on the morphological characteristics of circulating erythrocytes of the scorpion fish Scorpaena porcus (Linnaeus, 1758) has been investigated in an in vivo experiment. Under a 4-h adaptation of the fish to the conditions of ranked hypoxia their erythrocytes demonstrated a number of consecutive reactions. The volume and the...
Article
Предлагается новый метод идентификации азовского и черноморского подвидов европейского анчоуса Engraulis encrasicolus в смешанных зимовальных скоплениях на основе дискриминантного анализа с использованием морфологического критерия - угла центральной борозды отолита. Этот угол достоверно различается у данных подвидов: у азовского анчоуса его значени...
Article
Full-text available
Investigated the genetic characteristics of the 4 color groups of the bivalve mollusk Mytilus galloprovincilais (black, transitional, dark and light brown groups) identified by a method of computer processing photos. Using RAPD analysis, it was shown that the black sea populations of mussels are characterized by high genetic diversity. The presence...
Article
Full-text available
The effect of hypothermia on the content of myoglobin and cytochromes in the skeletal muscle of golden mullet (Liza aurata Risso) were investigation under the experimental conditions. Control group fish were kept at 15±1oC. The water temperature in the tanks was lowered at a speed of 0.2oC h-1 to 10±1; 5±1 and 1–2°C and examined the process of adap...
Article
Full-text available
Cell suspensions of head kidney and spleen of black scorpionfish (Scorpaena porcus L.) have been studied using flow cytometry and light microscopy. On the basis of forward scatter (FS) and side scatter (SS) distribution and light microscopy two main types of cells in the hemopoietic organs were identified: "small cells" (5,1-8,9 µm) and "large cell...
Article
Tissue specificity of the state of glutathione complex - reduced glutathione (GSH), activity of glutathione reductase (GR) and glutathione peroxidase (GP) - was investigated in the bivalve mollusk Ana-dara kagoshimensis (Tokunaga, 1906). Using high performance liquid chromatography, UV-VIS-spectra and mass spectra, 7 types of carotenoids (trans- an...
Article
Full-text available
The correlation of the state of glutathione complex composed of reduced glutathione (GSH), glutathione reductase (GR) activity and glutathione peroxidase (GP) and the qualitative composition of carotenoids was investigated in the bivalve mollusk Anadara kagoshimensis (Tokunaga, 1906). Using high-performance liquid chromatography, UV-Vis and mass sp...
Article
The investigation of the mechanisms of red blood cell steadiness to the oxygen lack in tolerant teleosts is of current scientific interest. Black scorpionfish, Scorpaena porcus L., is a widespread benthal species in the Black Sea and is highly resistant to hypoxic influence. The morphological state of black scorpionfish red blood cells under acute...