Hello, I have another question around this topic, Is it possible not to be able to amplify sequences carried by low-copy plasmids py classic PCR? Thanks in advance.
That depends on amount of the DNA and interfering compounds, how well your primers work and how many cycles of PCR you do.
In principle, you should be able o deec single copy of DNA. In practice, you do not have to get signal.
Aletheia Lima Souza
Universidade de Fortaleza
Malladi Sameer Kumar
Indian Institute of Science
University of Regina
University of California, San Diego
Erome Daniel Hankore
University of Nebraska at Lincoln
University of Groningen
Laura del Amo
Universidad Autónoma de Madrid
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