Question

# How to calculate concentration of 100uM ACETOSYRINGONE?

I need to prepare 100uM solution of acetosyringone for my co cultivation experimentsbut I'm confused in calculation of concentration, can someone please verify my calculations for 100uM acetosyringone? what amount of AS should be used to make 100ml of 100uM AS solution?

7th Jul, 2020
Alisha Alisha
I have just started working with Acetosyringone. I would suggest you prepare 100mM stock solution by adding 1.920gm in 100ml of DMSO. Then you can add say like 10ul in 100ml Media to make your final concentration of AS to be 100uM.
1 Recommendation

20th Mar, 2019
Yuan-Yeu Yau
1. Prepared 0.1M Actosyringone solution: (see link below)
2. To prepare 100 mL of 100 uM AS solution:
(1) 0.1 M stock = 100 mM = 100, 000 uM
(2) (100,000 uM) x (Y/(100 mL))= 100 uM
(3) Therefore, Y =0.1 mL = 100 uL.
(4) So, add 100 uL of stock solution to your 100 mL solution and you will get around 100 uM AS
3 Recommendations

20th Mar, 2019
Yuan-Yeu Yau
1. Prepared 0.1M Actosyringone solution: (see link below)
2. To prepare 100 mL of 100 uM AS solution:
(1) 0.1 M stock = 100 mM = 100, 000 uM
(2) (100,000 uM) x (Y/(100 mL))= 100 uM
(3) Therefore, Y =0.1 mL = 100 uL.
(4) So, add 100 uL of stock solution to your 100 mL solution and you will get around 100 uM AS
3 Recommendations
20th Mar, 2019
Mabrur Mabrur
Universitas Lambung Mangkurat
I'm suggest you following the isntruction by Yuan-Yew Yau. Preparing 100 mL of 100 uM AS Solution is very hard. You need to weigh 1.96202 mg of AS powder on analytical balances. That's why you need to make 0.1 M stock solution.
20th Mar, 2019
İbrahim Ertan Erkan
Isparta University of Applied Sciences
100uM =0,0001 M
M=m/ma*v 0,0001=m/196,19*0,1 so it makes 0,0019619 gr
congr. Dont forget forget to solve it with DMSO.
20th Mar, 2019
Naseeha Qureshi
National University of Sciences and Technology
Yuan-Yeu Yau, Mabrur Mabrur and İbrahim Ertan Erkan thankyou very much for your explict answers.
7th Jul, 2020
Alisha Alisha
I have just started working with Acetosyringone. I would suggest you prepare 100mM stock solution by adding 1.920gm in 100ml of DMSO. Then you can add say like 10ul in 100ml Media to make your final concentration of AS to be 100uM.
1 Recommendation

## Related Publications

Book
Full-text available
K.C. Samal., S.S. Beura, A. Mishra, U. Pradhan and A. Pradhan. 2013. Plant tissue culture technique and improved method of banana cultivation. Odisha University of Agriculture and Technology, Bhubaneswar
Chapter
Cabbages are a widely grown and economically important vegetable that were domesticated during the first millennium B.C. They belong to the highly diversified Brassicaceae family comprising about 3000 species. In North America and Europe, B. oleracea white cabbage (B. oleracea var. capitata) is the predominant agronomic type; whereas in Asia, Chine...
Got a technical question?